Journal of Applied Laboratory Medicine最新文献_第9页

Revisiting Chromosomal Translocations: Insights From Healthy Individuals and Lymphoma. 重新审视染色体易位：来自健康个体和淋巴瘤的见解。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-27 DOI: 10.1093/jalm/jfaf031

Sulaf Farhat Maghrabi, Zeina N Al-Mahayri, Fawza Monem

引用次数: 0

Use of Reference Change Values When Only 2 Analytical Results Are Known. 当只有两个分析结果已知时，参考变化值的使用。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-27 DOI: 10.1093/jalm/jfaf033

Arne Åsberg, Gustav Mikkelsen

引用次数: 0

Volumetric Absorptive Microsampling Combined with Mass Spectrometry to Support Pharmacokinetically-Guided Precision Dosing of Mycophenolate Mofetil in Pediatric Lupus Nephritis Patients. 体积吸收显微取样结合质谱法支持药代动力学指导下霉酚酸酯在小儿狼疮肾炎患者中的精确给药。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-26 DOI: 10.1093/jalm/jfaf022

Junfang Zhao, Xueheng Zhao, Tomoyuki Mizuno, Kei Irie, Prasad Devarajan, Hermine I Brunner, Kenneth D R Setchell

{"title":"Volumetric Absorptive Microsampling Combined with Mass Spectrometry to Support Pharmacokinetically-Guided Precision Dosing of Mycophenolate Mofetil in Pediatric Lupus Nephritis Patients.","authors":"Junfang Zhao, Xueheng Zhao, Tomoyuki Mizuno, Kei Irie, Prasad Devarajan, Hermine I Brunner, Kenneth D R Setchell","doi":"10.1093/jalm/jfaf022","DOIUrl":"https://doi.org/10.1093/jalm/jfaf022","url":null,"abstract":"Background: The immunosuppressant mycophenolate mofetil (MMF) is used off-label in patients with systemic lupus erythematosus (SLE) although the optimum dosing regimen is not well established. This study evaluated the use of volumetric absorptive microsampling (VAMS) for capillary whole blood collected by finger-prick, combined with tandem mass spectrometry and limited timepoint sampling to determine concentrations of mycophenolic acid (MPA) and its glucuronide conjugate, MPA-7-O-glucuronide (MPAG) in SLE patients. This approach permitted pharmacokinetically guided optimized dosing of MPA.Methods: Blood was collected by finger-prick and venipuncture from patients (n = 10) at trough, 30, and 120 min postdosing with MMF. MPA/MPAG concentrations were assayed from dried VAMS devices by stable-isotope dilution LC-MS-MS and compared to MPA/MPAG concentrations measured in plasma by high performance liquid chromatography after adjusting for hematocrit.Results: There was no significant difference between MPA concentrations from VAMS-collected dried capillary blood hematocrit-adjusted and those for plasma. The area under the concentration-time curve (AUC) estimated from plasma equivalent concentrations converted from capillary VAMS results correlated well with the AUC estimated from plasma concentrations (R2 = 0.97).Conclusions: The plasma pharmacokinetics of MMF metabolites can be reliably estimated from concentrations in capillary blood using VAMS devices and only 3 timed collections. Sampling whole blood by finger-prick, a less invasive approach for patients, coupled with the specificity of LC-MS/MS can be accurately used as an alternative to plasma sampling to establish the optimal dosing regimen of MMF for patients with SLE based on dried blood samples.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on Can Hemoglobin A1c Be Greater Than 100%? A Case of Homozygous Hemoglobin J-Iran. 糖化血红蛋白可以大于100%吗？纯合血红蛋白j -伊朗1例。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-25 DOI: 10.1093/jalm/jfaf020

Sharon M Geaghan

引用次数: 0

The 2024 PhD Board-Certified Clinical Chemist Compensation Survey. 2024 年博士学位委员会认证临床化学家薪酬调查。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-24 DOI: 10.1093/jalm/jfaf017

Jaime H Noguez, Anna E Merrill, Adam J McShane, Yachana Kataria

{"title":"The 2024 PhD Board-Certified Clinical Chemist Compensation Survey.","authors":"Jaime H Noguez, Anna E Merrill, Adam J McShane, Yachana Kataria","doi":"10.1093/jalm/jfaf017","DOIUrl":"https://doi.org/10.1093/jalm/jfaf017","url":null,"abstract":"Background: Board-certified clinical chemists with doctor of philosophy (PhD) degrees are essential to laboratory medicine and healthcare, yet comprehensive data on their total compensation is limited. The Association for Diagnostics & Laboratory Medicine (ADLM, formerly AACC) Society for Young Clinical Laboratorians Core Committee conducted its fifth compensation survey in April 2024, providing updated data on salary, benefits, and job satisfaction among board-certified clinical chemists.Methods: The 2024 compensation survey was distributed to all doctoral-level ADLM members based in the United States and Canada (n = 1576). Confidential, self-reported data were collected from respondents on various demographic and professional characteristics, including academic degree, board certification, years of experience, employment sector, total compensation (defined as base salary plus bonus), geographic location, race/ethnicity, and sex. The study included a total of 291 respondents for analysis.Results: Ninety-two percent (n = 267) of the respondents resided in the United States and 90% held a PhD degree. More than half of the respondents were employed in academic, hospital, or healthcare system settings. Among those with board certification, 86% were certified by the American Board of Clinical Chemistry (ABCC). The median total compensation for ABCC board-certified PhD clinical chemists working in the United States (n = 158) was $220 000 to $229 000.Conclusions: The 2024 survey highlights a continued upward trend in compensation for board-certified PhD clinical chemists, reflecting their expertise and contributions to healthcare. This report serves as a critical resource for professionals to advocate for equitable and competitive compensation as well as benefits that support career advancement and development.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can Hb A1c Be Greater Than 100%? A Case of Homozygous Hemoglobin J-Iran. 糖化血红蛋白可以大于100%吗？纯合血红蛋白j -伊朗1例。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-19 DOI: 10.1093/jalm/jfaf014

Maximo J Marin, Shruti S Vaghasia, Molly W Mandernach, Neil S Harris

引用次数: 0

AI-Augmented Kidney Stone Composition Analysis with Auto-Release Improves Quality, Efficiency, Cost-Effectiveness, and Staff Satisfaction. 具有自动释放功能的人工智能增强肾结石成分分析提高了质量、效率、成本效益和员工满意度。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-03 DOI: 10.1093/jalm/jfae146

Patrick L Day, Denise Rokke, Laura Schneider, Jillian Abbott, Brenda Holmen, Patrick Johnson, Mikolaj A Wieczorek, Katie L Kunze, Rickey E Carter, Joshua Bornhorst, Paul J Jannetto

{"title":"AI-Augmented Kidney Stone Composition Analysis with Auto-Release Improves Quality, Efficiency, Cost-Effectiveness, and Staff Satisfaction.","authors":"Patrick L Day, Denise Rokke, Laura Schneider, Jillian Abbott, Brenda Holmen, Patrick Johnson, Mikolaj A Wieczorek, Katie L Kunze, Rickey E Carter, Joshua Bornhorst, Paul J Jannetto","doi":"10.1093/jalm/jfae146","DOIUrl":"10.1093/jalm/jfae146","url":null,"abstract":"Background: We sought to evaluate key performance indicators related to an internally developed and deployed artificial intelligence (AI)-augmented kidney stone composition test system for potential improvements in test quality, efficiency, cost-effectiveness, and staff satisfaction.Methods: We compared quality, efficiency, staff satisfaction, and financial data from the 6 months after the AI-augmented laboratory test system was deployed (test period) with data from the same 6-month period in the previous year (control period) to determine if AI-augmentation improved key performance indicators of this laboratory test.Results: In the 6 months following the deployment (test period) of the AI-augmented kidney stone composition test system, 44 830 kidney stones were analyzed. Of these, 92% of kidney stones were eligible for AI-assisted interpretation. Out of these AI-eligible stones, 45% were able to be auto-released by the AI-augmented test system without human secondary review. Furthermore, the new AI-augmented kidney stone test system resulted in an apparent 40% reduction in incorrect laboratory results. Additionally, the new AI-augmented test system improved laboratory efficiency by 20%, improved staff satisfaction, and reduced the average analysis cost per kidney stone by $0.23.Conclusions: The AI-augmented test system improved test quality, efficiency, cost-effectiveness and staff satisfaction related to this kidney stone composition test.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"305-314"},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presumptive Identification of Clinically Significant Hemoglobin Variants Hb E, Hb S, Hb D in Hb A1c Capillary Electrophoresis. 在 Hb A1c 毛细管电泳中推定识别具有临床意义的血红蛋白变异体 Hb E、Hb S 和 Hb D。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-03 DOI: 10.1093/jalm/jfae102

Ankitha K Puthiyaveettil, Glen S Vaz, Sujay R Prasad, Deepalakshmi D Putchen

{"title":"Presumptive Identification of Clinically Significant Hemoglobin Variants Hb E, Hb S, Hb D in Hb A1c Capillary Electrophoresis.","authors":"Ankitha K Puthiyaveettil, Glen S Vaz, Sujay R Prasad, Deepalakshmi D Putchen","doi":"10.1093/jalm/jfae102","DOIUrl":"10.1093/jalm/jfae102","url":null,"abstract":"Background: The quantitation of glycated hemoglobin (Hb A1c) represents an average blood glucose level for a period of 2 to 3 months for diagnosing, monitoring, and managing diabetes mellitus. Unreliable results are reported when hemoglobin (Hb) variants are present in the sample. Patients are advised to use an alternate method due to the presence of the variant Hb and a reflex test to Hb electrophoresis to obtain precise information about the Hb variant. The present study utilizes x axis values from Hb A1c capillary electrophoresis (CE) to identify clinically significant Hb variants Hb E, S, and D.Methods: Patient samples (n = 60) that showed a variant peak in the Hb A1c test with an x axis value of 190 to 240 were selected for the study. The migration position of the Hb variant (x axis value) and variant percent of the Hb A1c test were compared with the x axis value and variant percent in the Hb electrophoresis test to presumptively identify the variants. The identity of the variants was confirmed using mass spectrometry (MS).Results: Out of 60 samples, 20 samples were identified as Hb E (x axis 225-227), 20 samples were identified as Hb S (x axis 210-214), and 18 samples were identified as Hb D-Punjab (x axis 200-201). Two variants with an x axis value of 194 were identified as an α variant Hb Q India using MS. There is an overall negative shift of the x axis (-1 to -13 units) and a lower variant percent (-0.2% to -8.7%) in Hb A1c CE when compared with Hb electrophoresis.Conclusions: The present study highlights the significance of the x axis value and variant percent to identify clinically significant Hb variants in the Hb A1c CE test.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"406-415"},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algorithm for the Identification of Hemoglobin Wayne Interference on Hb A1c Measurement Using Intact Hemoglobin Protein Mass Spectrometry Analysis. 使用完整血红蛋白蛋白质谱分析法识别血红蛋白韦恩干扰 Hb A1c 测量的算法。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-03 DOI: 10.1093/jalm/jfae109

Yu Zi Zheng, Adam J McShane, Sihe Wang, Sarah Ondrejka, Jessica M Colón-Franco

引用次数: 0

Preanalytical Sedimentation Effects on Hematological Tests. 分析前沉降对血液学试验的影响。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-03-03 DOI: 10.1093/jalm/jfae148

Kenneth Gard, Ian L Gunsolus

{"title":"Preanalytical Sedimentation Effects on Hematological Tests.","authors":"Kenneth Gard, Ian L Gunsolus","doi":"10.1093/jalm/jfae148","DOIUrl":"10.1093/jalm/jfae148","url":null,"abstract":"Introduction: Specimens suspected of errors related to low hemoglobin or changes in hemoglobin beyond that of clinically explained variations during hospital stays are frequently redrawn under the auspices that they are contaminated. When lack of an indwelling IV eliminates contamination as a possibility, evaluation of the specimen between the time of collection and testing should occur.Methods: As part of a quality improvement project, we investigated the impact of sedimentation on collected blood specimens not immediately transferred to their respective tubes from a syringe. Each syringe of blood was allowed to stand in a vertical position on the transfer device. After 10 min, each syringe was divided into a bottom half and top half into fresh blood tubes, with half of the samples inverted prior to division. Samples were then analyzed for complete blood count.Results: These results indicate that implementing an inversion of collected specimens prior to transferring will effectively eliminate variations related to sedimentation of blood.Conclusions: Results highlight the importance of specimen handling after collection, including appropriate mixing to avoid erroneous results caused by erythrocyte sedimentation. Mixing syringes before transferring blood to collection tubes ensures a uniform sample and accurate result.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"455-458"},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0