Journal of Applied Laboratory Medicine最新文献_第8页

Analytical Interference in Alanine Aminotransferase Determination in Patients with Monoclonal Gammopathy. 单克隆γ病患者丙氨酸转氨酶测定的分析干扰。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-26 DOI: 10.1093/jalm/jfaf115

Sara Martínez-Rodríguez, Alberto Izquierdo-Martínez, Juan Miguel Guerrero Montávez, Ricardo Rubio-Sánchez

{"title":"Analytical Interference in Alanine Aminotransferase Determination in Patients with Monoclonal Gammopathy.","authors":"Sara Martínez-Rodríguez, Alberto Izquierdo-Martínez, Juan Miguel Guerrero Montávez, Ricardo Rubio-Sánchez","doi":"10.1093/jalm/jfaf115","DOIUrl":"https://doi.org/10.1093/jalm/jfaf115","url":null,"abstract":"Background: Analytical interferences are very common in clinical laboratories, so professionals must develop strategies for their detection, avoiding incorrect results that can lead to inappropriate diagnoses and treatments.Methods: An isolated 1040 error (absorbance-related) in the Alanine Aminotransferase2 (ALT2) assay performed on the Abbott Alinity c that occurred in 158 samples over 7 months was investigated. Highly lipemic or hemolyzed samples were excluded, and an error due to an increased concentration of total proteins was ruled out, all of which are documented analytical interferences.Results: We isolated immunoglobulin (Ig) with an increased concentration (monoclonal components: 149 IgM, 7 IgG, and 2 IgA) from all analyzed samples, so the presence of this error solely in the alanine aminotransferase (ALT) assay had a 100% positive predictive value for monoclonal gammopathy. Serum viscosity was elevated in all cases, which is the reason for the detected interference. Treatment of IgM samples with dithiothreitol confirmed that dissociation of the pentamers eliminates the error in ALT determination.Conclusions: The detection of this interference in samples from patients without recent immunoglobulin determinations indicates the presence of a significant and isolated increase in the concentration of one of them, potentially leading to the diagnosis of a previously unknown monoclonal gammopathy.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on Persistent Low Vancomycin Concentration: An Endogenous Interference. 持续低万古霉素浓度：一种内源性干扰。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-26 DOI: 10.1093/jalm/jfaf117

Imir G Metushi

引用次数: 0

Persistent Low Vancomycin Concentration: An Endogenous Interference. 持续低万古霉素浓度：一种内源性干扰。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-26 DOI: 10.1093/jalm/jfaf107

Tomas Gajdos, Anissa Meskal, Marian Piqueur

引用次数: 0

ortho/meta/para-Methylacetylfentanyl-Induced Unique Interferences in Fentanyl Urine Drug Testing with Immunoassay and LC-MS/MS. 邻位/间位/对甲基乙酰芬太尼诱导的芬太尼尿药物免疫检测和LC-MS/MS的独特干扰

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-21 DOI: 10.1093/jalm/jfaf116

David A Barajas, Heather C Noda Carter, Michael R Tomedi, Gregory D Reynolds, Hieu T Dinh, Marisol S Castaneto, Pucheng Ke

引用次数: 0

Total Prostate-Specific Antigen Testing in Capillary Samples: Back to the Future. 总前列腺特异性抗原检测在毛细管样品：回到未来。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-21 DOI: 10.1093/jalm/jfaf113

Miyo K Chatanaka, Maria Pascual Lorén, Eleftherios P Diamandis

引用次数: 0

In Reply to Total Prostate-Specific Antigen Testing in Capillary Samples: Back to the Future. 对毛细管样品中总前列腺特异性抗原检测的回应：回到未来。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-21 DOI: 10.1093/jalm/jfaf114

Ravinder Sodi

引用次数: 0

First Laboratory Evaluation of FUS-3000 Plus: A New-Generation Urine Analyzer. 新一代尿液分析仪FUS-3000 Plus的首次实验室评估。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-20 DOI: 10.1093/jalm/jfaf111

Yasmine Nezzar, Elena Lazarova, Monia Chemais

{"title":"First Laboratory Evaluation of FUS-3000 Plus: A New-Generation Urine Analyzer.","authors":"Yasmine Nezzar, Elena Lazarova, Monia Chemais","doi":"10.1093/jalm/jfaf111","DOIUrl":"https://doi.org/10.1093/jalm/jfaf111","url":null,"abstract":"Background: Urine sediment analysis is a cornerstone of diagnostic testing. This study evaluates FUS-3000 Plus, an automated urine sediment analyzer using advanced imaging and artificial intelligence, to assess its technical performance and diagnostic accuracy for routine clinical use.Methods: The study analyzed 98 urine samples for chemical parameters (pH, protein, blood, leukocyte esterase, and nitrite) and 76 samples for particle analysis (red blood cells [RBCs], white blood cells, epithelial cells, crystals, bacteria) by both FUS-3000 Plus and sediMAX™, the current laboratory analyzer in use. Additionally, 139 samples were tested for glucosuria and proteinuria, with results compared to the Cobas C702. Carry-over, precision, and linearity were assessed by internal quality controls in accordance with Clinical and Laboratory Standards Institute protocols. Accuracy was further evaluated using external quality controls.Results: FUS-3000 Plus demonstrated strong agreement with sediMAX for nitrites, protein, and leukocyte esterase (kappa values >0.5) and correlated well with the Cobas C702 for glucosuria and proteinuria. However, discrepancies were observed in glucosuria detection, with some samples yielding inaccurate results even during external quality control assessments. A carry-over effect for RBCs required a rinse step after highly concentrated samples.Precision was acceptable (CV: 3%-11%), and Bland-Altman plots showed strong agreement for formed elements (correlation >0.95). However, the analyzer had reduced accuracy in bacteriuria detection.Conclusion: FUS-3000 Plus is a reliable tool for routine urinalysis, excelling in particle classification. However, improvements are needed in bacteriuria detection and minimizing carry-over effects. Future research should explore its ability to identify additional cellular elements and its diagnostic utility in diverse clinical populations.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating Updated Fentanyl Immunoassays for Loperamide Interference. 评价更新的芬太尼免疫分析法对洛哌丁胺干扰的影响。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-20 DOI: 10.1093/jalm/jfaf110

Michael E Walsh, Grace R Williams, Paul J Jannetto, K Aaron Geno

{"title":"Evaluating Updated Fentanyl Immunoassays for Loperamide Interference.","authors":"Michael E Walsh, Grace R Williams, Paul J Jannetto, K Aaron Geno","doi":"10.1093/jalm/jfaf110","DOIUrl":"https://doi.org/10.1093/jalm/jfaf110","url":null,"abstract":"Background: Loperamide is a µ-opioid receptor agonist that reduces intestinal peristalsis and is used to treat diarrhea. We previously described significant cross-reactivity of loperamide with 2 fentanyl immunoassays. Since then, new fentanyl immunoassays, including a CLIA-waived point-of-care device, have been approved for clinical use.Methods: We evaluated new fentanyl immunoassays for cross-reactivity to loperamide and its major metabolites, N-desmethyl loperamide (dLop) and N-didesmethyl loperamide (ddLop). Previously characterized assays were tested for cross-reactivity to ddLop, which recently became commercially available. Loperamide, dLop, and ddLop were spiked in drug-free urine for analysis by 5 enzyme immunoassays run on automated chemistry analyzers and one lateral flow assay for the detection of fentanyl.Results: Loperamide and its metabolites produced positive results in 3 fentanyl immunoassays. The Immunalysis HEIA was previously determined to be reactive to both loperamide and dLop, but it was not reactive to ddLop. The Immunalysis SEFRIA was reactive to loperamide, dLop, and ddLop at minimum concentrations of 14.7 mg/L, 13.1 mg/L, and 17.0 mg/L. The Thermo Fisher DRI was previously determined to be reactive to loperamide and dLop, and it was reactive to ddLop at a minimum concentration of 33.1 mg/L. The Abbott iCassette, ARK Fentanyl II, and Lin-Zhi LZI II fentanyl assays showed no cross-reactivity to loperamide or its metabolites.Conclusions: The cross-reactivity of loperamide, dLop, and ddLop in several fentanyl immunoassays has the potential to cause false-positive results during urine drug screening.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of a Digital Automated Cell Morphology Analyzer Results for Determining Differential White Blood Cell Counts in a Turkish Pediatric Population. 利用数字自动细胞形态学分析仪的结果来确定在土耳其儿科人群中白细胞计数的差异。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-20 DOI: 10.1093/jalm/jfaf108

Elif Güler Kazancı, Yasemin Üstündağ, Meryem Rümeysa Yeşil, Hatice Azra Çağlak, Kağan Huysal, Deniz Güven, Gökalp Rüstem Aksoy, Sena Kazancı

{"title":"Utilization of a Digital Automated Cell Morphology Analyzer Results for Determining Differential White Blood Cell Counts in a Turkish Pediatric Population.","authors":"Elif Güler Kazancı, Yasemin Üstündağ, Meryem Rümeysa Yeşil, Hatice Azra Çağlak, Kağan Huysal, Deniz Güven, Gökalp Rüstem Aksoy, Sena Kazancı","doi":"10.1093/jalm/jfaf108","DOIUrl":"https://doi.org/10.1093/jalm/jfaf108","url":null,"abstract":"Background: Manual morphological analysis of peripheral blood smears (PBS) with light microscopy is an essential diagnostic and monitoring tool. Recently, automated morphology analyzers have been developed that can preclassify cells using artificial intelligence algorithms. This study aims to evaluate the preliminary leukocyte classification capabilities of the MC-80 digital morphology analyzer, a novel system, in pediatric patients and compare its performance with that of manual microscopy, the current gold standard.Methods: This retrospective study was conducted at SBU Bursa Yüksek İhtisas Training and Research Hospital between September 5 and 29, 2022. Blood samples from 153 consecutive pediatric patients (age range: 0-18 years; median age: 3 years) undergoing simultaneous hemograms and PBS analyses were assessed using both the MC-80 digital morphology analyzer and manual microscopy.Results: Spearman rank correlation coefficients indicated a high correlation for neutrophils (rho = 0.742; 95% CI: 0.661-0.807) and lymphocytes (rho = 0.745; 95% CI: 0.666-0.810) while the correlation for blast cells was significantly lower (rho = 0.079; 95% CI: -0.099-0.238). Concordance between the MC-80 and manual microscopy was minimal for monocytes (κ = 0.21; 95% CI: 0.11-0.29) and negligible for blast cells (κ = 0.08; 95% CI: 0.00-0.17).Conclusions: The MC-80 digital morphology analyzer shows acceptable preliminary classification for neutrophils and lymphocytes; further development is required before it can be routinely implemented in laboratory workflows.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Total Prostate-Specific Antigen (PSA) Testing in Capillary Samples: Proof-of-Principle and Feasibility Study for Home Self-Testing for Prostate Cancer. 修正：总前列腺特异性抗原（PSA）检测毛细管样品：原理证明和可行性研究的家庭自我检测前列腺癌。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-13 DOI: 10.1093/jalm/jfaf083

引用次数: 0