Journal of Applied Laboratory Medicine最新文献_第7页

Use of Reference Change Values When Only 2 Analytical Results Are Known. 当只有两个分析结果已知时，参考变化值的使用。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf033

Arne Åsberg, Gustav Mikkelsen

引用次数: 0

Unmeasurable Total Bilirubin in an 80-Year-Old Man. 80岁男性总胆红素不可测。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf079

Najmul Aufa Mohamad Zulkipli, Yin Ye Lai, Subashini C Thambiah, Intan Nureslyna Samsudin, Muhamad Syahmi Nazli

引用次数: 0

Commentary on Iatrogenic Maternal and Neonatal Hyperthyroidism. 医源性孕产妇和新生儿甲状腺功能亢进症综述。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf068

Zahra Shajani-Yi

引用次数: 0

Plasma Cell Enrichment and New Genomic Approaches in Multiple Myeloma: A Scoping Review. 浆细胞富集和新的基因组方法在多发性骨髓瘤：范围综述。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf044

Juan Javier López Rivera, Natalia Gomez-Lopera, Diana Jennifer Moreno-Garcia, Rocío Orduz-Rodriguez, Juan F Combariza-Vallejo, Mario Isaza-Ruget

{"title":"Plasma Cell Enrichment and New Genomic Approaches in Multiple Myeloma: A Scoping Review.","authors":"Juan Javier López Rivera, Natalia Gomez-Lopera, Diana Jennifer Moreno-Garcia, Rocío Orduz-Rodriguez, Juan F Combariza-Vallejo, Mario Isaza-Ruget","doi":"10.1093/jalm/jfaf044","DOIUrl":"10.1093/jalm/jfaf044","url":null,"abstract":"Background: Multiple myeloma (MM) is a genetically heterogeneous disease where specific genetic abnormalities have a significant impact on a patient's prognosis. Diagnostic and prognostic tools like fluorescence in situ hybridization (FISH), PCR, microarrays, and next-generation sequencing (NGS) have transformed MM management. However, the effectiveness of these techniques is often limited by the low concentration of plasma cells in bone marrow samples, which makes enrichment methods necessary. This review aims to clarify how these techniques enhance the detection of genetic abnormalities, reduce false-negative results, and facilitate more precise risk stratification for MM patients.Content: Following Preferred Reporting Items for Systematic reviews and Meta-Analyses Extension for Scoping Review (PRISMA-ScR) guidelines, the literature on plasma cell separation methods used in genetic studies of MM was systematically identified and mapped. Searches were conducted in the Medline and Embase databases using a structured strategy, supplemented by manual searches on Google Scholar. Of 399 publications evaluated, 69 met the inclusion criteria; 37% utilized FISH and 19% demonstrated an increasing use of NGS. Plasma cell enrichment significantly improved diagnostic accuracy, increasing the detection rates of genetic abnormalities from 61% in non-enriched samples to 95.5% in enriched samples. While FISH remains the gold standard, emerging technologies such as NGS offer superior sensitivity and the ability to identify critical genetic alterations to refine molecular subtypes.Summary: Clinically significant genetic alterations are detected more frequently with plasma cell enrichment techniques, contributing to improved prognosis and treatment strategies for MM patients.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"1292-1310"},"PeriodicalIF":1.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iatrogenic Maternal and Neonatal Hyperthyroidism. 医源性产妇和新生儿甲状腺功能亢进。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf029

Maiar Elghobashy, Harit Buch, Rousseau Gama

引用次数: 0

An Unusual Presentation of Falsely Elevated Free T4 Levels in a Young Woman. 一位年轻女性游离T4水平异常升高。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf056

Álvaro Cabrera-Argany, Paula Rodríguez-Sánchez-Pérez, Adexe J Fulgencio-González, Mauro Boronat

引用次数: 0

Lipid Ratio Plot: A Simple Graphical Approach for Investigating the Accuracy of LDL Cholesterol Equations or Direct Assays. 脂质比率图：用于调查低密度脂蛋白胆固醇方程或直接测定的准确性的简单图形方法。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf093

Thando Gcingca, Maureen Sampson, Rafael Zubirán, Anna Wolska, Jeffrey Meeusen, Leslie Donato, Allan Jaffe, Alan Remaley

{"title":"Lipid Ratio Plot: A Simple Graphical Approach for Investigating the Accuracy of LDL Cholesterol Equations or Direct Assays.","authors":"Thando Gcingca, Maureen Sampson, Rafael Zubirán, Anna Wolska, Jeffrey Meeusen, Leslie Donato, Allan Jaffe, Alan Remaley","doi":"10.1093/jalm/jfaf093","DOIUrl":"10.1093/jalm/jfaf093","url":null,"abstract":"Background: β-Quantification (BQ) is the reference method for low-density lipoprotein cholesterol (LDL-C) determination. It is not widely available, making it challenging for laboratories to assess the accuracy of LDL-C methods. Our goal was to develop an indirect graphical approach for comparing LDL-C test results to the BQ reference method.Methods: BQ results from Mayo Medical Laboratories (n = 39 969) and the National Institutes of Health (n = 17 825) were used to investigate the interrelationships between lipid panel tests. A plot of LDL-C/non high-density lipoprotein cholesterol (nonHDL-C) vs (triglyceride (TG)/nonHDL-C)0.5 (lipid ratio plot) resulted in a negative linear regression line (y = -34.2x + 115). Based on simulation analysis, the minimum sample size for calculating its slope and intercept with a coefficient of variation of about 7.5% was 80.Results: The regression lines for the lipid ratio plot of LDL-C calculated by the Sampson-NIH (y = -37.6x + 118) and enhanced Sampson-NIH (y = -33.1x + 116) equations closely overlapped with the BQ method. In contrast, the Martin-Hopkins equation showed a positive bias with an increasing TG/nonHDL-C ratio and exceeded the recommended bias limit of 4% on hypertriglyceridemic samples (y = -25.5x + 107). The Friedewald equation showed an even larger negative bias with hypertriglyceridemia (y = -47.5x + 126). Lipid ratio plots of the Roche direct assay revealed a fixed positive bias of approximately 4% (y = -33.9x + 120), whereas a much larger proportional positive bias was observed with increasing triglycerides for the Beckman direct assay (y = -14.6x + 97.8).Conclusions: The lipid ratio plot is a simple graphical approach that can be readily performed by clinical laboratories for investigating the accuracy of LDL-C assays by calculation methods or direct assays.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"1154-1167"},"PeriodicalIF":1.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144691977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood Storage in a Vaccine Carrier Eliminates the Need for Onsite Analysis of Major Hematological and Nutritional Biomarkers. 血液储存在疫苗载体中消除了现场分析主要血液学和营养生物标志物的需要。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-03 DOI: 10.1093/jalm/jfaf103

Layal S Jaafar, Razan S ZeinEddine, Zaher Chakhachiro, Omar A Obeid

{"title":"Blood Storage in a Vaccine Carrier Eliminates the Need for Onsite Analysis of Major Hematological and Nutritional Biomarkers.","authors":"Layal S Jaafar, Razan S ZeinEddine, Zaher Chakhachiro, Omar A Obeid","doi":"10.1093/jalm/jfaf103","DOIUrl":"10.1093/jalm/jfaf103","url":null,"abstract":"Background: Reliable hemoglobin (Hb) measurement is crucial for accurate estimation of anemia prevalence in population surveys. While portable meters like HemoCue devices are commonly used, studies show discrepancies in Hb readings. In fact, Hb assessment using automated hematology analyzers remains the most accurate method. However, their use typically requires transporting blood samples to centralized laboratories, which raises concerns about sample stability and result accuracy. Vaccine carriers are frequently utilized in micronutrient surveys for sample storage. Accordingly, this study aims to mimic field settings by evaluating the stability of various hematological and nutritional biomarkers in blood samples stored in vaccine carriers.Methods: Around 30 mL of venous blood was collected from fasting subjects and subsequently distributed into potassium 2-ethylene-diamine-tetraacetic acid microtubes for complete blood count measurement, and serum separator tubes for serum biomarker analysis. Samples were stored in vaccine carriers for 48 h and analyzed at 6 time points (0, 6, 12, 24, 36, and 48 h). The mean % deviation of parameters from baseline was compared to the total change limit (TCL), and values within the TCL were considered clinically stable.Results: Most hematological parameters and nutritional biomarkers remained stable when stored in vaccine carriers for up to 24 h. Only white blood cell counts and ferritin levels showed clinically significant changes.Conclusion: Our findings suggest that using vaccine carriers to store blood samples is an adequate alternative to refrigerators, especially in field settings.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"1188-1199"},"PeriodicalIF":1.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving Pleural Fluid pH Accuracy: A Quality Improvement Initiative. 提高胸膜液pH值准确性：一项质量改进倡议。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-02 DOI: 10.1093/jalm/jfaf118

Sydney M Bowmaster, Anna E Merrill, Nicole L Manning, Jeffrey S Wilson, Thomas J Gross

{"title":"Improving Pleural Fluid pH Accuracy: A Quality Improvement Initiative.","authors":"Sydney M Bowmaster, Anna E Merrill, Nicole L Manning, Jeffrey S Wilson, Thomas J Gross","doi":"10.1093/jalm/jfaf118","DOIUrl":"https://doi.org/10.1093/jalm/jfaf118","url":null,"abstract":"Background: Fluid pH remains a standard test when evaluating pleural effusions. Studies recommend using a blood gas analyzer to measure fluid pH immediately after sample collection. Centralization of laboratory services at our institution led to migration of blood gas analysis to a single core laboratory with uncertain impacts on pH results.Methods: Pleural fluid samples were obtained from 30 patients during routine clinical care, excluding those with frank purulence or blood. Fluid pH and pCO2 were measured on a blood gas analyzer. We compared samples collected in plastic screw-top vials from commercially available thoracentesis kits vs paired samples collected in blood gas syringes. We also examined how differences in processing time, fluid pCO2, and heparin anticoagulant correlated with measured pH.Results: Of the 30 paired samples obtained, 25 (83.3%) were exudates and 5 (16.7%) were transudates. All samples collected in blood gas syringes had lower mean pH and higher mean pCO2 than samples collected in plastic screw-top vials (mean pH difference ± SD, 0.16 ± 0.06; P < 0.001) (mean pCO2 difference ± SD, 14.09 ± 6.92; P < 0.001). Differences in fluid pH were independent of total nucleated cell count (range 65-28 380 cells/mm3). Neither the presence of heparin in the syringe nor time delays up to 2 h between collection and analysis meaningfully impacted the pH result (P = 0.51 and P = 0.75, respectively).Conclusion: To accurately measure pleural fluid pH, specimens should be collected anaerobically in blood gas syringes rather than the screw-top vials provided in thoracentesis kits.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Reference Values of Ceruloplasmin across the Adult Age Range in a Large Italian Healthy Population. 更正：在意大利健康人群的成年年龄范围内铜蓝蛋白的参考值。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-08-29 DOI: 10.1093/jalm/jfaf122

引用次数: 0