Journal of Applied Laboratory Medicine最新文献

The Preanalytical Process Matters: Impact of Blood Collection Tubes on the Measurement of Vitamin D Using Liquid Chromatography Tandem Mass Spectrometry. 前分析过程事项：血液采集管对使用液相色谱串联质谱法测量维生素D的影响。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-10-07 DOI: 10.1093/jalm/jfaf141

Sae Ochi, Akihiro Kunisawa, Tomokazu Matsuura, Hiroyasu Miyamoto, Yuichi Ikeda, Masaki Miyasaka, Ayasa Tajima, Reiko Kisugi, Daisuke Kawakami, Tsuyoshi Nakanishi, Nobuhiro Hanafusa, Yutaka Furutani

{"title":"The Preanalytical Process Matters: Impact of Blood Collection Tubes on the Measurement of Vitamin D Using Liquid Chromatography Tandem Mass Spectrometry.","authors":"Sae Ochi, Akihiro Kunisawa, Tomokazu Matsuura, Hiroyasu Miyamoto, Yuichi Ikeda, Masaki Miyasaka, Ayasa Tajima, Reiko Kisugi, Daisuke Kawakami, Tsuyoshi Nakanishi, Nobuhiro Hanafusa, Yutaka Furutani","doi":"10.1093/jalm/jfaf141","DOIUrl":"https://doi.org/10.1093/jalm/jfaf141","url":null,"abstract":"Background: Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a highly sensitive analytical tool that has become the gold standard for measuring the concentrations of some steroidal hormones and therapeutic drugs in blood. However, the application of this technology in different clinical laboratories faces challenges related to the preanalytical process. In this study, we investigated how the materials used in blood collection tubes affect the measurement of serum 25-hydroxyvitamin D [25(OH)D] by LC-MS/MS.Methods: Serum samples were collected in 20 different blood collection tubes made by various manufacturers. The samples were processed under controlled conditions to simulate clinical practice, followed by measurement using an LC-MS/MS system. The data were compared with measurements obtained from a chemiluminescent enzyme immunoassay.Results: Significant variability in measurement outcomes was observed depending on the type of blood collection tube used. Specific blood collection tubes from a manufacturer that employed specific separator gels exhibited interference, as indicated by broad peaks in chromatograms. This interference complicated the quantification of 25(OH)D. The interference was consistent across multiple samples, indicating a systemic problem related to the materials used in the blood collection tubes.Conclusions: These findings highlight the need to evaluate blood collection tubes from various manufacturers prior to the clinical implementation of LC-MS/MS measurements. Because LC-MS/MS has unique potential error sources, our results indicate the importance in utilizing LC-MS/MS international standards to ensure accurate and reliable results in clinical laboratories.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reevaluating Ferritin Reference Intervals: Defining Limits Based on Iron-Sufficient Population Studies, Clinical Relevance, and Healthcare Efficiency. 重新评估铁蛋白参考区间：基于铁充足人群研究、临床相关性和医疗保健效率确定限度。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-10-06 DOI: 10.1093/jalm/jfaf151

Jillian Kodger, Joe El-Khoury, Nalan Yurtsever

引用次数: 0

Evolving Insights Regarding Biomarkers to Better Manage Older Adults With Cardiovascular Disease. 关于生物标志物的不断发展的见解，以更好地管理老年人心血管疾病。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-10-03 DOI: 10.1093/jalm/jfaf156

Mohamad B Moumneh, Daniel E Forman, Abdulla A Damluji, Christopher R deFilippi

{"title":"Evolving Insights Regarding Biomarkers to Better Manage Older Adults With Cardiovascular Disease.","authors":"Mohamad B Moumneh, Daniel E Forman, Abdulla A Damluji, Christopher R deFilippi","doi":"10.1093/jalm/jfaf156","DOIUrl":"https://doi.org/10.1093/jalm/jfaf156","url":null,"abstract":"Background: Aging is associated with subclinical changes in cardiac structure and function as well as an increase in prevalent cardiovascular disease and geriatric syndromes such as frailty and sarcopenia. This can result in levels of cardiac-specific and non-cardiac-specific circulating biomarkers that are frequently above normal range concentrations established in healthy middle-age general population cohorts in the absence of acute disease. Without this recognition, clinicians may be challenged to interpret biomarker results in older adults in the setting of diagnosing an acute illness or for longer term prognostication.Content: In this review, we provide anticipated findings, their suggested underlying mechanism, as well as interpretation of concentrations for the common cardiovascular biomarkers including cardiac troponins and natriuretic peptides in the acute care and ambulatory settings. Specifically, information to interpret biomarkers in the setting of possible acute myocardial infarction and heart failure is presented. We also present data for interpreting results in older adults with other well-known prognostic biomarkers, as well as biomarkers with application to geriatric syndromes.Summary: Circulating biomarkers, despite challenges in interpretation in older adults relative to younger adults, play a critical role in the diagnosis and prognosis of cardiovascular disease and have additional roles in geriatric syndromes and assessing risk of harm from specific treatments.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Analytical Evaluation of an Immunoassay for Long Forms of Cardiac Troponin T. 长型心肌肌钙蛋白T免疫测定法的设计与分析评价。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-30 DOI: 10.1093/jalm/jfaf143

Rami Aalto, Akseli Lahtinen, K E Juhani Airaksinen, Tuija Vasankari, Tapio Hellman, Laura Koskimäki, Saara Wittfooth

{"title":"Design and Analytical Evaluation of an Immunoassay for Long Forms of Cardiac Troponin T.","authors":"Rami Aalto, Akseli Lahtinen, K E Juhani Airaksinen, Tuija Vasankari, Tapio Hellman, Laura Koskimäki, Saara Wittfooth","doi":"10.1093/jalm/jfaf143","DOIUrl":"https://doi.org/10.1093/jalm/jfaf143","url":null,"abstract":"Background: Cardiac troponins (cTn) are used as diagnostic biomarkers of acute myocardial infarction (MI), but elevated levels of cTn can also be observed in other conditions. We report here the design and analytical evaluation of an immunoassay that targets intact and mildly fragmented forms of cardiac troponin T (referred to as long cTnT), which has been shown to better differentiate between MI and end-stage renal disease than the current Roche Elecsys® high sensitivity cTnT assay.Methods: The long cTnT assay was evaluated for analytical characteristics. Serum and heparin plasma sample matrices were compared and the analyte stability was studied by storing endogenous long cTnT from samples of ST-segment elevation MI patients in heparin plasma or buffer at different temperatures and subjecting samples to freeze-thaw cycles. The correlation of long cTnT levels and time after MI symptom onset was also studied.Results: The long cTnT assay achieved a limit of detection of 10.8 ng/L and a lower limit of quantitation (10% CV) of 30.8 ng/L. The response was linear from 5 to 5000 ng/L. Serum produced significantly lower results than heparin plasma. Endogenous long cTnT tolerated freeze-thaw cycles, but stability was compromised when stored at higher temperatures. The fraction of circulating long cTnT was highest during early hours of MI.Conclusion: The long cTnT assay presented good analytical performance. Our results support using heparin plasma as the sample material and avoiding prolonged sample storing at room temperatures. Long cTnT fraction decreases in time after the onset of type 1 MI. ClinicalTrials.gov registration: NCT04465591.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145193411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Protein Glycosylation on the Measurement of Soluble Urokinase Plasminogen Activator Receptor. 蛋白糖基化对可溶性尿激酶纤溶酶原激活物受体测定的影响。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-29 DOI: 10.1093/jalm/jfaf147

Jaya Montecillo, Richard W Troughton, A Mark Richards, Christopher J Pemberton, Janice Chew-Harris

{"title":"Influence of Protein Glycosylation on the Measurement of Soluble Urokinase Plasminogen Activator Receptor.","authors":"Jaya Montecillo, Richard W Troughton, A Mark Richards, Christopher J Pemberton, Janice Chew-Harris","doi":"10.1093/jalm/jfaf147","DOIUrl":"https://doi.org/10.1093/jalm/jfaf147","url":null,"abstract":"Background: Elevated concentrations of the glycoprotein soluble urokinase plasminogen activator receptor (suPAR) predict worse cardiovascular disease (CVD) outcomes. However, glycosylation effects on its measurement are unknown.Methods: Plasma samples were obtained from healthy volunteers (n = 70), patients with and without myocardial infarction (MI) from an acute chest pain cohort (n = 65), and patients with and without acute decompensated heart failure (ADHF) from an acute breathlessness cohort (n = 103). After the addition of either deglycosylation enzymes or a diluent to paired samples from each patient, subsequent measurements for suPAR were undertaken with the suPARnostic assay. Percentage change in concentrations between enzyme-treated and matched nontreated samples was calculated. MI and ADHF discrimination by receiver operating characteristic area under the curve was used to compare performances of nontreated suPAR vs deglycosylated suPAR.Results: Following deglycosylation, measured suPAR concentrations are increased by a median of 52.8% (from 1.9 to 3.0 ng/mL, P < 0.0001) in healthy subjects. Similar increases for deglycosylated suPAR were observed in MI (53.6%) and non-MI acute chest pain patients (53.3%). Although acutely breathless patients obtained smaller increases in deglycosylated suPAR values than healthy individuals (P < 0.0001), the percentage increase was higher in ADHF (38.6%) compared to non-HF (24.4%, P = 0.002) patients. ADHF discrimination was superior for deglycosylated suPAR than non-treated suPAR (0.850 vs 0.765, P = 0.003), but no differences were observed for MI discrimination (0.485 vs 0.501, P = 0.508).Conclusions: Glycosylation underestimates suPAR measurement using the suPARnostic assay. Although glycosylation effects varied across patient groups, removal of suPAR glycosylation appears to enhance heart failure discrimination.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blister and Bite Red Blood Cells as Diagnostic Beacons in Acute Hemolytic Anemia. 水泡和咬伤红细胞作为急性溶血性贫血的诊断指标。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-29 DOI: 10.1093/jalm/jfaf140

Roopa Kodimyala, Samir B Kahwash, Jingcai Wang

引用次数: 0

Commentary on Blister and Bite Red Blood Cells as Diagnostic Beacons in Acute Hemolytic Anemia. 水疱和咬伤红细胞作为急性溶血性贫血诊断指标的研究进展。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-29 DOI: 10.1093/jalm/jfaf148

Kristie White

引用次数: 0

Blood Utilization and Waste Following Implementation of Thromboelastography. 血栓弹性成像后的血液利用和浪费。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-26 DOI: 10.1093/jalm/jfaf139

Kaitlyn M Shelton, LeeAnn P Walker, Carol A Carman, Daniel González, Sarah Burnett-Greenup

{"title":"Blood Utilization and Waste Following Implementation of Thromboelastography.","authors":"Kaitlyn M Shelton, LeeAnn P Walker, Carol A Carman, Daniel González, Sarah Burnett-Greenup","doi":"10.1093/jalm/jfaf139","DOIUrl":"https://doi.org/10.1093/jalm/jfaf139","url":null,"abstract":"Background: Thromboelastography (TEG) is a viscoelastic testing platform that monitors real-time coagulation. This study evaluated changes in blood use and waste following TEG implementation, as well as changes in massive transfusion protocol (MTP) blood utilization and returns following TEG implementation.Methods: The study is a retrospective analysis of blood component transfusion in 3 services (cardiothoracic surgery, emergency department, and trauma surgery) and overall blood waste at a level II trauma center for 104 weeks before and after TEG implementation. Blood utilization and waste were compared by a paired t-test. Additionally, MTP blood utilization and returns were compared for 80 cases pre-TEG and 80 cases post-TEG implementation by an independent t-test.Results: Changes in weekly mean utilization after implementation of TEG differed among component types. Red blood cell (RBC) transfusions increased by 15% (P < 0.01), while plasma transfusions decreased by 26% (P < 0.05). The mean RBC units wasted decreased (P < 0.01), but the mean plasma units wasted increased significantly between the pre- and post-TEG groups (P < 0.001). The weekly mean platelet and cryoprecipitate waste and utilization showed no significant difference between the pre- and post-TEG groups. MTP utilization and returns showed no significant difference between pre- and post-TEG implementation.Conclusions: In line with previous literature, plasma utilization decreased post-TEG implementation. Plasma waste unexpectedly increased, which may be due to an increase of orders for plasma that were not transfused. RBC utilization and waste showed significant changes, although the impact of blood availability during the COVID pandemic should be considered a confounding variable in this study.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Suspicion of IVIG Interference to Diagnosis of Rare Syndrome. 从怀疑IVIG干扰到罕见综合征的诊断。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-18 DOI: 10.1093/jalm/jfaf134

Sercan Erol, Evin Kocatürk, Özben Ö Işıklar, Hava Ü Teke

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Commentary on From Suspicion of Intravenous Immunoglobin Interference to Diagnosis of Rare Syndrome. 从怀疑静脉注射免疫球蛋白干扰到诊断罕见综合征述评。

IF 1.9

Journal of Applied Laboratory Medicine Pub Date : 2025-09-18 DOI: 10.1093/jalm/jfaf142

Mary Kathryn Bohn

引用次数: 0