Journal of Applied Laboratory Medicine最新文献_第2页

Commentary on Two Different Pigments in the Peritoneal Fluid of a Child: What Is the Clinical Significance of Each? 儿童腹膜液中两种不同色素的临床意义？

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-14 DOI: 10.1093/jalm/jfaf043

Sharon Markham Geaghan

引用次数: 0

Two Different Pigments in the Peritoneal Fluid of a Child: What is the Clinical Significance of Each? 儿童腹膜液中两种不同的色素：每种色素的临床意义是什么？

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-14 DOI: 10.1093/jalm/jfaf036

Jingcai Wang, Dustin R Bunch, Samir Kahwash

引用次数: 0

Clinical and Molecular Characteristics of a Patient with 11-Beta-Hydroxylase Deficiency. 11- β -羟化酶缺乏症患者的临床和分子特征。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-09 DOI: 10.1093/jalm/jfaf038

Malgorzata Monika Brzozowska, Jeremy Allen Knott, Laura Buckton, Andrea Rita Horvath, David J Handelsman

引用次数: 0

Commentary on Clinical and Molecular Characteristics of Patient with 11-Beta-Hydroxylase Deficiency. 11- β -羟化酶缺乏症患者的临床和分子特征评述。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-09 DOI: 10.1093/jalm/jfaf049

Sharon Markham Geaghan

引用次数: 0

Reference Intervals for Placental Biomarkers in Early Pregnancy. 妊娠早期胎盘生物标志物的参考区间。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-09 DOI: 10.1093/jalm/jfaf064

Maja Hjelm Lundgaard, Niels Henrik Bruun, Aase Handberg, Stig Andersen, Stine Linding Andersen

{"title":"Reference Intervals for Placental Biomarkers in Early Pregnancy.","authors":"Maja Hjelm Lundgaard, Niels Henrik Bruun, Aase Handberg, Stig Andersen, Stine Linding Andersen","doi":"10.1093/jalm/jfaf064","DOIUrl":"https://doi.org/10.1093/jalm/jfaf064","url":null,"abstract":"Background: Placental dysfunction is important to recognize, and more evidence is needed on the dynamics of the placental biomarkers, soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF), in early pregnancy. This study aimed to establish reference intervals for placental biomarkers in early pregnancy.Methods: This was a retrospective cohort study of pregnant women (n = 702) in the North Denmark Region, 2013, who had blood samples drawn in early pregnancy including measurements of sFlt-1 and PlGF (Kryptor Compact, Thermofisher Scientific). Reference intervals were established, and the association between sFlt-1 and PlGF and maternal characteristics (age, body mass index [BMI], country of birth, and smoking in pregnancy) was evaluated using linear regression analyses and reported as adjusted beta coefficient (aβ) with 95% confidence intervals (CIs).Results: The placental biomarkers showed a dynamic trend with higher levels for increasing week of pregnancy; however, sFlt-1 reached a plateau around week 10 of pregnancy. Higher maternal age associated with higher sFlt-1 and PlGF (sFlt-1: aβ 1.02 [95% CI, 1.01-1.03], PlGF: aβ 1.02 [95% CI, 1.01-1.03]), and higher maternal BMI associated with lower sFlt-1 (aβ 0.97 [95% CI, 0.96-0.98]). Furthermore, the level of PlGF was higher among women born outside of Denmark (aβ 1.17 [95% CI, 1.03-1.34]) compared to women born in Denmark and among smokers (aβ 1.56 [95% CI, 1.38-1.78]) compared to nonsmokers.Conclusions: In a large cohort of pregnant women in Denmark, levels of sFlt-1 and PlGF increased during early pregnancy and were influenced by a series of maternal characteristics.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference Range Determination for the sFlt-1/PlGF Ratio in a Diverse Cohort of Pregnant Women in the United States. 美国不同孕妇队列中sFlt-1/PlGF比值的测定

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-07 DOI: 10.1093/jalm/jfaf063

Antonio F Saad, David M Haas, Eugene Chang, Scott A Shainker, Miriah Denbo, Michelle Silasi, George Saade, Sarosh Rana, Tetsuya Kawakita, Luis D Pacheco, Jennifer Powers Carson, Angela Dietl, Deirdre Allegranza, Ge Guo, David Stamilio

{"title":"Reference Range Determination for the sFlt-1/PlGF Ratio in a Diverse Cohort of Pregnant Women in the United States.","authors":"Antonio F Saad, David M Haas, Eugene Chang, Scott A Shainker, Miriah Denbo, Michelle Silasi, George Saade, Sarosh Rana, Tetsuya Kawakita, Luis D Pacheco, Jennifer Powers Carson, Angela Dietl, Deirdre Allegranza, Ge Guo, David Stamilio","doi":"10.1093/jalm/jfaf063","DOIUrl":"https://doi.org/10.1093/jalm/jfaf063","url":null,"abstract":"Background: The serum soluble fms-like tyrosine kinase-1 (sFlt-1) to placental growth factor (PlGF) ratio is an established tool for predicting preeclampsia in many countries but is not widely adopted in the United States. This study aimed to determine reference ranges for the Elecsys® sFlt-1/PlGF ratio and the sFlt-1 and PlGF immunoassays (Roche Diagnostics International Ltd) in a diverse US population of healthy pregnant women.Methods: This was a prospective, noninterventional sample and data collection study performed between November 2022 and November 2023 at 9 collection sites and one measurement site in the United States. Evaluable women were ≥18 years old, apparently healthy, between 23 + 0 to 40 + 6 gestational weeks with a singleton pregnancy and had one serum sample collected. Reference ranges for 23 + 0 to 40 + 6 gestational weeks and 5 gestational windows within this range were determined using a nonparametric method.Results: Of 818 recruited women, 561 were evaluable for the analysis and were representative of the US population in terms of self-reported race, ethnicity, and maternal age. Reference ranges for the sFlt-1/PlGF ratio, as determined by the 2.5th and 97.5th percentiles (90% CIs), were 0.9 (0.7, 1.0) to 13.5 (11.6, 14.9) for a US intended use population at 23 + 0 to 34 + 6 gestational weeks (n = 380) and 0.9 (0.7, 1.1) to 40.8 (29.0, 47.2) for the entire evaluable population at 23 + 0 to 40 + 6 gestational weeks.Conclusions: Reference ranges were determined for sFlt-1/PlGF and the sFlt-1 and PlGF immunoassays in a diverse, healthy US population of pregnant women at 23 + 0 to 40 + 6 gestational weeks.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Prevalence of Xylazine in Patient Urine Samples That Were Positive for Fentanyl in Western New York. 纽约西部芬太尼阳性患者尿样中氯拉嗪的流行。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-02 DOI: 10.1093/jalm/jfae158

Brandy L Young, Putuma P Gqamana, Yinghua Qiu, Nicholas E Nacca, Y Victoria Zhang

{"title":"The Prevalence of Xylazine in Patient Urine Samples That Were Positive for Fentanyl in Western New York.","authors":"Brandy L Young, Putuma P Gqamana, Yinghua Qiu, Nicholas E Nacca, Y Victoria Zhang","doi":"10.1093/jalm/jfae158","DOIUrl":"10.1093/jalm/jfae158","url":null,"abstract":"Background: Xylazine, a veterinary sedative, is increasingly being discovered in the illegal drug supply across the United States and is associated with overdose fatalities. Not approved for human use, xylazine poses life-threatening risks, particularly when used in conjunction with opioids such as fentanyl. This study evaluates the prevalence of xylazine in urine samples that screened positive for fentanyl. The evaluation involved measuring the parent drug xylazine and its metabolites, xylazine glucuronide and hydroxy-xylazine, as well as finding their correlation with fentanyl and norfentanyl concentrations in patient samples.Methods: Samples were collected over a one-month period. Urine samples were analyzed for fentanyl, norfentanyl, and 3 xylazine-specific analytes (i.e., xylazine, xylazine glucuronide, and hydroxy-xylazine). Briefly, reverse-phase chromatographic separation was performed on a Bonshell Phenyl-Hexyl column utilizing a binary mobile phase gradient. The eluents were detected through positive electrospray ionization and multiple reaction monitoring analysis on a triple quadrupole mass spectrometer.Results: Out of a total of 230 urine samples, 184 were confirmed positive for fentanyl. Xylazine was detected in 56 out of the 184 fentanyl-positive samples, accounting for 30% of the fentanyl-positive confirmatory test. Xylazine (or its metabolites) was not observed in fentanyl-negative samples. Furthermore, the parent xylazine drug was detected in all 56 samples, whereas the metabolite glucuronide and hydroxy forms were only detected in 28 and 6 samples, respectively.Conclusions: This study estimated a xylazine prevalence of 30% in fentanyl-positive urine samples within our local Western New York population. This study represents the first report within our clinical population.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"572-582"},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Crystals in Gram-Stained Synovial Fluid. 革兰氏染色滑液中晶体的检测。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-02 DOI: 10.1093/jalm/jfae162

Steven J Szlembarski, William A Agger, Sarah R Pendley, Stephen J Bloechl, Christopher H Cogbill, Arick P Sabin, Cathy Mikkelson Fischer, Douglas W White

{"title":"Detection of Crystals in Gram-Stained Synovial Fluid.","authors":"Steven J Szlembarski, William A Agger, Sarah R Pendley, Stephen J Bloechl, Christopher H Cogbill, Arick P Sabin, Cathy Mikkelson Fischer, Douglas W White","doi":"10.1093/jalm/jfae162","DOIUrl":"10.1093/jalm/jfae162","url":null,"abstract":"Background: Gout and calcium pyrophosphate deposition (CPPD) disease are common crystalline arthropathies. Identification of crystals in synovial fluid using polarized light microscopy (PLM) aids diagnosis. In routine clinical practice, the volume of synovial fluid available for PLM is frequently insufficient, especially when fluid is also needed for other analyses, such as Gram stain and culture. Few studies have investigated the reliability of microscopic examination for crystals, with or without polarization, using Gram-stained synovial fluid specimens. This study estimated the sensitivity and specificity of microscopy with and without polarization on Gram-stained specimens for identification of crystals compared with standard PLM on unstained samples.Methods: In total, 105 unstained synovial and bursal fluid samples were processed and examined by laboratory personnel using routine PLM procedures. Gram-stained samples of the same fluid were independently interpreted by microbiologists trained in examining fluid for crystals using standard light microscopy, followed by at least 2 pathologists using PLM.Results: Compared with standard PLM on unstained samples, the sensitivity and specificity of crystal examination by microbiologists on Gram-stained samples were 16% and 98%, respectively. Pathologists, using PLM on these Gram-stained samples, achieved slightly better sensitivity (24.5% and 96%, respectively). Concordance regarding crystal type was 100% and unaffected by Gram stain.Conclusions: Crystal examination of Gram-stained synovial and bursal fluid by trained personnel has low sensitivity but high specificity compared with standard evaluation of unstained samples. Examination of Gram-stained samples with PLM may be helpful when sample volumes are limited and, when positive, has sufficient specificity to support crystalline arthropathy diagnoses.","PeriodicalId":46361,"journal":{"name":"Journal of Applied Laboratory Medicine","volume":" ","pages":"653-658"},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Unexpected Cause of Rhabdomyolysis. 横纹肌溶解的意外原因。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-02 DOI: 10.1093/jalm/jfae168

Manon Robert, Justine Blin, Alizée Violin, Kalyane Bach-Ngohou, Damien Masson

引用次数: 0

Fostering Research Collaborations as an Early Career Clinical Laboratory Director. 作为早期职业临床实验室主任促进研究合作。

IF 1.8

Journal of Applied Laboratory Medicine Pub Date : 2025-05-02 DOI: 10.1093/jalm/jfaf008

Erica M Fatica, Christopher D Koch

引用次数: 0