APL Bioengineering最新文献_第5页

Electrodynamic interaction between tumor treating fields and microtubule electrophysiological activities. 肿瘤治疗场与微管电生理活动之间的电动相互作用

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-03 eCollection Date: 2024-06-01 DOI: 10.1063/5.0197900

Xing Li, Kaida Liu, Haohan Fang, Zirong Liu, Yuchen Tang, Ping Dai

{"title":"Electrodynamic interaction between tumor treating fields and microtubule electrophysiological activities.","authors":"Xing Li, Kaida Liu, Haohan Fang, Zirong Liu, Yuchen Tang, Ping Dai","doi":"10.1063/5.0197900","DOIUrl":"10.1063/5.0197900","url":null,"abstract":"Tumor treating fields (TTFields) are a type of sinusoidal alternating current electric field that has proven effective in inhibiting the reproduction of dividing tumor cells. Despite their recognized impact, the precise biophysical mechanisms underlying the unique effects of TTFields remain unknown. Many of the previous studies predominantly attribute the inhibitory effects of TTFields to mitotic disruption, with intracellular microtubules identified as crucial targets. However, this conceptual framework lacks substantiation at the mesoscopic level. This study addresses the existing gap by constructing force models for tubulin and other key subcellular structures involved in microtubule electrophysiological activities under TTFields exposure. The primary objective is to explore whether the electric force or torque exerted by TTFields significantly influences the normal structure and activities of microtubules. Initially, we examine the potential effect on the dynamic stability of microtubule structures by calculating the electric field torque on the tubulin dimer orientation. Furthermore, given the importance of electrostatics in microtubule-associated activities, such as chromosome segregation and substance transport of kinesin during mitosis, we investigate the interaction between TTFields and these electrostatic processes. Our data show that the electrodynamic effects of TTFields are most likely too weak to disrupt normal microtubule electrophysiological activities significantly. Consequently, we posit that the observed cytoskeleton destruction in mitosis is more likely attributable to non-mechanical mechanisms.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"026118"},"PeriodicalIF":6.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11151432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burst sine wave electroporation (B-SWE) for expansive blood-brain barrier disruption and controlled non-thermal tissue ablation for neurological disease. 正弦波脉冲电穿孔（B-SWE）用于扩张性血脑屏障破坏和神经系统疾病的可控非热组织消融。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-05-30 eCollection Date: 2024-06-01 DOI: 10.1063/5.0198382

Sabrina N Campelo, Zaid S Salameh, Julio P Arroyo, James L May, Sara O Altreuter, Jonathan Hinckley, Rafael V Davalos, John H Rossmeisl

{"title":"Burst sine wave electroporation (B-SWE) for expansive blood-brain barrier disruption and controlled non-thermal tissue ablation for neurological disease.","authors":"Sabrina N Campelo, Zaid S Salameh, Julio P Arroyo, James L May, Sara O Altreuter, Jonathan Hinckley, Rafael V Davalos, John H Rossmeisl","doi":"10.1063/5.0198382","DOIUrl":"10.1063/5.0198382","url":null,"abstract":"The blood-brain barrier (BBB) limits the efficacy of treatments for malignant brain tumors, necessitating innovative approaches to breach the barrier. This study introduces burst sine wave electroporation (B-SWE) as a strategic modality for controlled BBB disruption without extensive tissue ablation and compares it against conventional pulsed square wave electroporation-based technologies such as high-frequency irreversible electroporation (H-FIRE). Using an in vivo rodent model, B-SWE and H-FIRE effects on BBB disruption, tissue ablation, and neuromuscular contractions are compared. Equivalent waveforms were designed for direct comparison between the two pulsing schemes, revealing that B-SWE induces larger BBB disruption volumes while minimizing tissue ablation. While B-SWE exhibited heightened neuromuscular contractions when compared to equivalent H-FIRE waveforms, an additional low-dose B-SWE group demonstrated that a reduced potential can achieve similar levels of BBB disruption while minimizing neuromuscular contractions. Repair kinetics indicated faster closure post B-SWE-induced BBB disruption when compared to equivalent H-FIRE protocols, emphasizing B-SWE's transient and controllable nature. Additionally, finite element modeling illustrated the potential for extensive BBB disruption while reducing ablation using B-SWE. B-SWE presents a promising avenue for tailored BBB disruption with minimal tissue ablation, offering a nuanced approach for glioblastoma treatment and beyond.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"026117"},"PeriodicalIF":6.0,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11149061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk fibroin promotes H3K9me3 expression and chromatin reorganization to regulate endothelial cell proliferation. 蚕丝纤维素促进 H3K9me3 表达和染色质重组，从而调节内皮细胞增殖。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-05-29 eCollection Date: 2024-06-01 DOI: 10.1063/5.0203858

Kaixiang Gao, Yafan Xie, Fangning Xu, Qin Peng, Li Fu, Guixue Wang, Juhui Qiu

{"title":"Silk fibroin promotes H3K9me3 expression and chromatin reorganization to regulate endothelial cell proliferation.","authors":"Kaixiang Gao, Yafan Xie, Fangning Xu, Qin Peng, Li Fu, Guixue Wang, Juhui Qiu","doi":"10.1063/5.0203858","DOIUrl":"10.1063/5.0203858","url":null,"abstract":"Silk fibroin (SF), which is extensively utilized in tissue engineering and vascular grafts for enhancing vascular regeneration, has not been thoroughly investigated for its epigenetic effects on endothelial cells (EC). This study employed RNA sequencing analysis to evaluate the activation of histone modification regulatory genes in EC treated with SF. Subsequent investigations revealed elevated H3K9me3 levels in SF-treated EC, as evidenced by immunofluorescence and western blot analysis. The study utilized H2B-eGFP endothelial cells to demonstrate that SF treatment results in the accumulation of H2B-marked chromatin in the nuclear inner cavities of EC. Inhibition of H3K9me3 levels by a histone deacetylase inhibitor TSA decreased cell proliferation. Furthermore, the activation of the MAPK signaling pathway using chromium picolinate decreased the proliferative activity and H3K9me3 level in SF-treated EC. SF also appeared to enhance cell growth and proliferation by modulating the H3K9me3 level and reorganizing chromatin, particularly after oxidative stress induced by H2O2 treatment. In summary, these findings indicate that SF promotes EC proliferation by increasing the H3K9me3 level even under stress conditions.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"026115"},"PeriodicalIF":6.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testing mixing rules for structural and dynamical quantities in multi-component crowded protein solutions. 测试多组分拥挤蛋白质溶液中结构和动态量的混合规则。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-05-29 eCollection Date: 2024-06-01 DOI: 10.1063/5.0204201

Alessandro Gulotta, Saskia Bucciarelli, Felix Roosen-Runge, Olaf Holderer, Peter Schurtenberger, Anna Stradner

{"title":"Testing mixing rules for structural and dynamical quantities in multi-component crowded protein solutions.","authors":"Alessandro Gulotta, Saskia Bucciarelli, Felix Roosen-Runge, Olaf Holderer, Peter Schurtenberger, Anna Stradner","doi":"10.1063/5.0204201","DOIUrl":"10.1063/5.0204201","url":null,"abstract":"Crowding effects significantly influence the phase behavior and the structural and dynamic properties of the concentrated protein mixtures present in the cytoplasm of cells or in the blood serum. This poses enormous difficulties for our theoretical understanding and our ability to predict the behavior of these systems. While the use of course grained colloid-inspired models allows us to reproduce the key physical solution properties of concentrated monodisperse solutions of individual proteins, we lack corresponding theories for complex polydisperse mixtures. Here, we test the applicability of simple mixing rules in order to predict solution properties of protein mixtures. We use binary mixtures of the well-characterized bovine eye lens proteins α and γB crystallin as model systems. Combining microrheology with static and dynamic scattering techniques and observations of the phase diagram for liquid-liquid phase separation, we show that reasonably accurate descriptions are possible for macroscopic and mesoscopic signatures, while information on the length scale of the individual protein size requires more information on cross-component interaction.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"026116"},"PeriodicalIF":6.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guest Editorial: Implantable bioelectronics. 特约编辑：植入式生物电子学。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-05-28 eCollection Date: 2024-06-01 DOI: 10.1063/5.0209537

Yael Hanein, Josef Goding

{"title":"Guest Editorial: Implantable bioelectronics.","authors":"Yael Hanein, Josef Goding","doi":"10.1063/5.0209537","DOIUrl":"10.1063/5.0209537","url":null,"abstract":"The realm of implantable bioelectronics represents a frontier in medical science, merging technology, biology, and medicine to innovate treatments that enhance, restore, or monitor physiological functions. This field has yielded devices like cochlear implants, cardiac pacemakers, deep brain stimulators, and vagus nerve stimulators, each designed to address a specific health condition, ranging from sensorineural hearing loss to chronic pain, neurological disorders, and heart rhythm irregularities. Such devices underscore the potential of bioelectronics to significantly improve patient outcomes and quality of life. Recent technological breakthroughs in materials science, nanotechnology, and microfabrication have enabled the development of more sophisticated, smaller, and biocompatible bioelectronic devices. However, the field also encounters challenges, particularly in extending the capabilities of devices such as retinal prostheses, which aim to restore vision but currently offer limited visual acuity. Research in implantable bioelectronics is highly timely, driven by an aging global population with a growing prevalence of chronic diseases that could benefit from these technologies. The convergence of societal health needs, advancing technological capabilities, and a supportive ecosystem for innovation marks this era as pivotal for bioelectronic research.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"020401"},"PeriodicalIF":6.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11136517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A patient-specific echogenic soft robotic left ventricle embedded into a closed-loop cardiovascular simulator for advanced device testing. 嵌入闭环心血管模拟器的患者特异性回声软机器人左心室，用于先进设备测试。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-05-28 eCollection Date: 2024-06-01 DOI: 10.1063/5.0203653

Maria Rocchi, Konstantina Papangelopoulou, Marcus Ingram, Youri Bekhuis, Guido Claessen, Piet Claus, Jan D'hooge, Dirk W Donker, Bart Meyns, Libera Fresiello

{"title":"A patient-specific echogenic soft robotic left ventricle embedded into a closed-loop cardiovascular simulator for advanced device testing.","authors":"Maria Rocchi, Konstantina Papangelopoulou, Marcus Ingram, Youri Bekhuis, Guido Claessen, Piet Claus, Jan D'hooge, Dirk W Donker, Bart Meyns, Libera Fresiello","doi":"10.1063/5.0203653","DOIUrl":"10.1063/5.0203653","url":null,"abstract":"Cardiovascular medical devices undergo a large number of pre- and post-market tests before their approval for clinical practice use. Sophisticated cardiovascular simulators can significantly expedite the evaluation process by providing a safe and controlled environment and representing clinically relevant case scenarios. The complex nature of the cardiovascular system affected by severe pathologies and the inherently intricate patient-device interaction creates a need for high-fidelity test benches able to reproduce intra- and inter-patient variability of disease states. Therefore, we propose an innovative cardiovascular simulator that combines in silico and in vitro modeling techniques with a soft robotic left ventricle. The simulator leverages patient-specific and echogenic soft robotic phantoms used to recreate the intracardiac pressure and volume waveforms, combined with an in silico lumped parameter model of the remaining cardiovascular system. Three different patient-specific profiles were recreated, to assess the capability of the simulator to represent a variety of working conditions and mechanical properties of the left ventricle. The simulator is shown to provide a realistic physiological and anatomical representation thanks to the use of soft robotics combined with in silico modeling. This tool proves valuable for optimizing and validating medical devices and delineating specific indications and boundary conditions.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"026114"},"PeriodicalIF":6.6,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11136518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141176352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Air-liquid intestinal cell culture allows in situ rheological characterization of intestinal mucus. 通过气液肠细胞培养，可以对肠粘液进行原位流变学表征。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-05-07 eCollection Date: 2024-06-01 DOI: 10.1063/5.0187974

Pamela C Cai, Margaret Braunreuther, Audrey Shih, Andrew J Spakowitz, Gerald G Fuller, Sarah C Heilshorn

{"title":"Air-liquid intestinal cell culture allows in situ rheological characterization of intestinal mucus.","authors":"Pamela C Cai, Margaret Braunreuther, Audrey Shih, Andrew J Spakowitz, Gerald G Fuller, Sarah C Heilshorn","doi":"10.1063/5.0187974","DOIUrl":"10.1063/5.0187974","url":null,"abstract":"Intestinal health heavily depends on establishing a mucus layer within the gut with physical properties that strike a balance between being sufficiently elastic to keep out harmful pathogens yet viscous enough to flow and turnover the contents being digested. Studies investigating dysfunction of the mucus layer in the intestines are largely confined to animal models, which require invasive procedures to collect the mucus fluid. In this work, we develop a nondestructive method to study intestinal mucus. We use an air-liquid interface culture of primary human intestinal epithelial cells that exposes their apical surface to allow in situ analysis of the mucus layer. Mucus collection is not only invasive but also disrupts the mucus microstructure, which plays a crucial role in the interaction between mucus and the gut microbiome. Therefore, we leverage a noninvasive rheology technique that probes the mechanical properties of the mucus without removal from the culture. Finally, to demonstrate biomedical uses for this cell culture system, we characterize the biochemical and biophysical properties of intestinal mucus due to addition of the cytokine IL-13 to recapitulate the gut environment of Nippostrongylus brasiliensis infection.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 2","pages":"026112"},"PeriodicalIF":6.6,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nondestructive, quantitative viability analysis of 3D tissue cultures using machine learning image segmentation. 利用机器学习图像分割技术对三维组织培养物进行无损、定量的活力分析。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-03-28 eCollection Date: 2024-03-01 DOI: 10.1063/5.0189222

Kylie J Trettner, Jeremy Hsieh, Weikun Xiao, Jerry S H Lee, Andrea M Armani

{"title":"Nondestructive, quantitative viability analysis of 3D tissue cultures using machine learning image segmentation.","authors":"Kylie J Trettner, Jeremy Hsieh, Weikun Xiao, Jerry S H Lee, Andrea M Armani","doi":"10.1063/5.0189222","DOIUrl":"10.1063/5.0189222","url":null,"abstract":"Ascertaining the collective viability of cells in different cell culture conditions has typically relied on averaging colorimetric indicators and is often reported out in simple binary readouts. Recent research has combined viability assessment techniques with image-based deep-learning models to automate the characterization of cellular properties. However, further development of viability measurements to assess the continuity of possible cellular states and responses to perturbation across cell culture conditions is needed. In this work, we demonstrate an image processing algorithm for quantifying features associated with cellular viability in 3D cultures without the need for assay-based indicators. We show that our algorithm performs similarly to a pair of human experts in whole-well images over a range of days and culture matrix compositions. To demonstrate potential utility, we perform a longitudinal study investigating the impact of a known therapeutic on pancreatic cancer spheroids. Using images taken with a high content imaging system, the algorithm successfully tracks viability at the individual spheroid and whole-well level. The method we propose reduces analysis time by 97% in comparison with the experts. Because the method is independent of the microscope or imaging system used, this approach lays the foundation for accelerating progress in and for improving the robustness and reproducibility of 3D culture analysis across biological and clinical research.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 1","pages":"016121"},"PeriodicalIF":6.6,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10985731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Publisher's Note: "Magneto-responsive hyaluronan hydrogel for hyperthermia and bioprinting: Magnetic, rheological properties and biocompatibility" [APL Bioeng. 7, 036113 (2023)]. 勘误：出版者注："用于热疗和生物打印的磁响应透明质酸水凝胶：7, 036113 (2023)].

IF 6 3区医学

APL Bioengineering Pub Date : 2024-03-19 eCollection Date: 2024-03-01 DOI: 10.1063/5.0207818

L Vítková, N Kazantseva, L Musilová, P Smolka, K Valášková, K Kocourková, M Humeník, A Minařík, P Humpolíček, A Mráček, I Smolková

引用次数: 0

Differential roles of normal and lung cancer-associated fibroblasts in microvascular network formation. 正常和肺癌相关成纤维细胞在微血管网络形成中的不同作用

IF 6 3区医学

APL Bioengineering Pub Date : 2024-03-19 eCollection Date: 2024-03-01 DOI: 10.1063/5.0188238

Naveen R Natesh, Pankaj Mogha, Alan Chen, Scott J Antonia, Shyni Varghese

{"title":"Differential roles of normal and lung cancer-associated fibroblasts in microvascular network formation.","authors":"Naveen R Natesh, Pankaj Mogha, Alan Chen, Scott J Antonia, Shyni Varghese","doi":"10.1063/5.0188238","DOIUrl":"10.1063/5.0188238","url":null,"abstract":"Perfusable microvascular networks offer promising three-dimensional in vitro models to study normal and compromised vascular tissues as well as phenomena such as cancer cell metastasis. Engineering of these microvascular networks generally involves the use of endothelial cells stabilized by fibroblasts to generate robust and stable vasculature. However, fibroblasts are highly heterogenous and may contribute variably to the microvascular structure. Here, we study the effect of normal and cancer-associated lung fibroblasts on the formation and function of perfusable microvascular networks. We examine the influence of cancer-associated fibroblasts on microvascular networks when cultured in direct (juxtacrine) and indirect (paracrine) contacts with endothelial cells, discovering a generative inhibition of microvasculature in juxtacrine co-cultures and a functional inhibition in paracrine co-cultures. Furthermore, we probed the secreted factors differential between cancer-associated fibroblasts and normal human lung fibroblasts, identifying several cytokines putatively influencing the resulting microvasculature morphology and functionality. These findings suggest the potential contribution of cancer-associated fibroblasts in aberrant microvasculature associated with tumors and the plausible application of such in vitro platforms in identifying new therapeutic targets and/or agents that can prevent formation of aberrant vascular structures.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"8 1","pages":"016120"},"PeriodicalIF":6.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10959556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0