AIMS Microbiology最新文献

{"title":"Exploring fermentation with lactic acid bacteria as a pretreatment for enhancing antioxidant potential in broccoli stem powders.","authors":"M Alegría Serna-Barrera, Claudia Bas-Bellver, Lucía Seguí, Noelia Betoret, Cristina Barrera","doi":"10.3934/microbiol.2024013","DOIUrl":"10.3934/microbiol.2024013","url":null,"abstract":"<p><p>Fruit and vegetable industries face a major environmental challenge with food loss and waste. Broccoli stems, comprising 38% of the plant's total weight, are usually discarded by the industry producing fourth-range and ready-to-use products, despite being rich in antioxidants, vitamins, fiber, carotenoids, phenolic compounds, and glucosinolates. Addressing the challenge of reducing waste in this sector includes the production of stable and nutrient-concentrated powders, which can be consumed directly or used as ingredients in functional food formulation. This study investigated fermentation with lactic acid bacteria (<i>Limosilactobacillus reuteri</i>, <i>Lactiplantibacillus plantarum</i>, and <i>Lactobacillus salivarius</i>) as a pretreatment for enhancing antioxidant and probiotic potential in broccoli stem powders. Results showed maximum counts 24 h after inoculation, and no effect of the previous disruption intensity on microbial growth was observed. Fermenting broccoli stems for 24 h with the three microbial strains led to a significant increase in total phenols and flavonoids but to a general reduction in the samples' capacity to scavenge DPPH and ABTS free radicals. Overall, ground broccoli stems exhibited the most favorable antioxidant properties following the 24 h fermentation step. The subsequent freeze-drying and final grinding had minimal impact on the microbial population but significantly enhanced the extractability of the antioxidant compounds. This study offers a valuable reference for researchers and stakeholders exploring the development of new products and innovations from vegetable waste.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 2","pages":"255-272"},"PeriodicalIF":2.7,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141451878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review: Marine aquaculture impacts marine microbial communities.","authors":"Xiao Zhang, Jia Hua, Zule Song, Kejun Li","doi":"10.3934/microbiol.2024012","DOIUrl":"10.3934/microbiol.2024012","url":null,"abstract":"<p><p>Marine aquaculture is key for protein production but disrupts marine ecosystems by releasing excess feed and pharmaceuticals, thus affecting marine microbes. Though vital, its environmental impact often remains overlooked. This article delves into mariculture's effects on marine microbes, including bacteria, fungi, viruses, and antibiotic-resistance genes in seawater and sediments. It highlights how different mariculture practices-open, pond, and cage culture-affect these microbial communities. Mariculture's release of nutrients, antibiotics, and heavy metals alters the microbial composition, diversity, and functions. Integrated multi-trophic aquaculture, a promising sustainable approach, is still developing and needs refinement. A deep understanding of mariculture's impact on microbial ecosystems is crucial to minimize pollution and foster sustainable practices, paving the way for the industry's sustainable advancement.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 2","pages":"239-254"},"PeriodicalIF":2.7,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141451864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on utilization of <i>Bacillus</i> species as plant probiotics for different crops in adverse conditions.","authors":"Shubhra Singh, Douglas J H Shyu","doi":"10.3934/microbiol.2024011","DOIUrl":"10.3934/microbiol.2024011","url":null,"abstract":"<p><p>Plant probiotic bacteria are a versatile group of bacteria isolated from different environmental sources to improve plant productivity and immunity. The potential of plant probiotic-based formulations is successfully seen as growth enhancement in economically important plants. For instance, endophytic <i>Bacillus</i> species acted as plant growth-promoting bacteria, influenced crops such as cowpea and lady's finger, and increased phytochemicals in crops such as high antioxidant content in tomato fruits. The present review aims to summarize the studies of <i>Bacillus</i> species retaining probiotic properties and compare them with the conventional fertilizers on the market. Plant probiotics aim to take over the world since it is the time to rejuvenate and restore the soil and achieve sustainable development goals for the future. Comprehensive coverage of all the <i>Bacillus</i> species used to maintain plant health, promote plant growth, and fight against pathogens is crucial for establishing sustainable agriculture to face global change. Additionally, it will give the latest insight into this multifunctional agent with a detailed biocontrol mechanism and explore the antagonistic effects of <i>Bacillus</i> species in different crops.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"220-238"},"PeriodicalIF":4.8,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140208844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nocardiopsis synnemataformans</i> NBRM9, an extremophilic actinomycete producing extremozyme cellulase, using lignocellulosic agro-wastes and its biotechnological applications.","authors":"Mohamed H El-Sayed, Doaa A Elsayed, Abd El-Rahman F Gomaa","doi":"10.3934/microbiol.2024010","DOIUrl":"10.3934/microbiol.2024010","url":null,"abstract":"<p><p>Actinomycetes are an attractive source of lignocellulose-degrading enzymes. The search for actinomycetes producing extremozyme cellulase using cheap lignocellulosic waste remains a priority goal of enzyme research. In this context, the extremophilic actinomycete NBRM9 showed promising cellulolytic activity in solid and liquid assays. This actinomycete was identified as <i>Nocardiopsis synnemataformans</i> based on its phenotypic characteristics alongside phylogenetic analyses of 16S rRNA gene sequencing (OQ380604.1). Using bean straw as the best agro-waste, the production of cellulase from this strain was statistically optimized using a response surface methodology, with the maximum activity (13.20 U/mL) achieved at an incubation temperature of 40 °C, a pH of 9, an incubation time of 7 days, and a 2% substrate concentration. The partially purified cellulase (PPC) showed promising activity and stability over a wide range of temperatures (20-90 °C), pH values (3-11), and NaCl concentrations (1-19%), with optimal activity at 50 °C, pH 9.0, and 10% salinity. Under these conditions, the enzyme retained >95% of its activity, thus indicating its extremozyme nature. The kinetics of cellulase showed that it has a V_max of 20.19 ± 1.88 U/mL and a Km of 0.25 ± 0.07 mM. The immobilized PPC had a relative activity of 69.58 ± 0.13%. In the in vitro microtiter assay, the PPC was found to have a concentration-dependent anti-biofilm activity (up to 85.15 ± 1.60%). Additionally, the fermentative conversion of the hydrolyzed bean straw by <i>Saccharomyces cerevisiae</i> (KM504287.1) amounted to 65.80 ± 0.52% of the theoretical ethanol yield. Overall, for the first time, the present work reports the production of extremozymatic (thermo, alkali-, and halo-stable) cellulase from <i>N. synnemataformans</i> NBRM9. Therefore, this strain is recommended for use as a biotool in many lignocellulosic-based applications operating under harsh conditions.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"187-219"},"PeriodicalIF":4.8,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of <i>Arthrobacter</i> strains isolated from Great Gobi A Strictly Protected Area, Mongolia.","authors":"Alberto Bernacchi, Giulia Semenzato, Manuel di Mascolo, Sara Amata, Angela Bechini, Fabiola Berti, Carmela Calonico, Valentina Catania, Giovanni Emiliani, Antonia Esposito, Claudia Greco, Stefano Mocali, Nadia Mucci, Anna Padula, Antonio Palumbo Piccionello, Battogtokh Nasanbat, Gantulga Davaakhuu, Munkhtsetseg Bazarragchaa, Francesco Riga, Claudio Augugliaro, Anna Maria Puglia, Marco Zaccaroni, Fani Renato","doi":"10.3934/microbiol.2024009","DOIUrl":"10.3934/microbiol.2024009","url":null,"abstract":"<p><p>Desert soil hosts many microorganisms, whose activities are essential from an ecological viewpoint. Moreover, they are of great anthropic interest. The knowledge of extreme environments microbiomes may be beneficial for agriculture, technology, and human health. In this study, 11 <i>Arthrobacter</i> strains from topsoil samples collected from the Great Gobi A Strictly Protected Area in the Gobi Desert, were characterized by a combination of different techniques. The phylogenetic analysis, performed using their 16S rDNA sequences and the most similar <i>Arthrobacter</i> sequences found in databases, revealed that most of them were close to <i>A. crystallopoietes</i>, while others joined a sister group to the clade formed by <i>A. humicola, A. pascens</i>, and <i>A. oryzae</i>. The resistance of each strain to different antibiotics, heavy-metals, and NaCl was also tested as well as the inhibitory potential against human pathogens (i.e., <i>Burkholderia</i> ssp., <i>Klebsiella pneumoniae, Pseudomonas aeruginosa</i>, and <i>Staphylococcus</i> ssp.) via cross-streaking, to check the production of metabolites with antimicrobial activity. Data obtained revealed that all strains were resistant to heavy metals and were able to strongly interfere with the growth of many of the human pathogens tested. The volatile organic compounds (VOCs) profile of the 11 <i>Arthrobacter</i> strains was also analyzed. A total of 16 different metabolites were found, some of which were already known for having an inhibitory action against different Gram-positive and Gram-negative bacteria. Isolate MS-3A13, producing the highest quantity of VOCs, is the most efficient against <i>Burkholderia cepacia</i> complex (Bcc), <i>K. pneumoniae</i>, and coagulase-negative Staphylococci (CoNS) strains. This work highlights the importance of understanding microbial populations' phenotypical characteristics and dynamics in extreme environments to uncover the antimicrobial potential of new species and strains.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"161-186"},"PeriodicalIF":4.8,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic analysis of fecal samples in colorectal cancer Egyptians patients post colectomy: A pilot study.","authors":"Rana H Abo-Hammam, Mohammed Salah, Sarah Shabayek, Amro Hanora, Samira Zakeer, Randa H Khattab","doi":"10.3934/microbiol.2024008","DOIUrl":"10.3934/microbiol.2024008","url":null,"abstract":"<p><p>One of the most prevalent malignancies that significantly affects world health is colorectal cancer (CRC). While genetics are involved in a portion of CRC patients, most cases are sporadic. The microbiome composition could be a new source of tumor initiation and progression. This research was conducted to investigate the microbiota composition of CRC patients post colectomy at taxonomic and functional levels. Using a next-generation sequencing approach, using an Illumina Novaseq 6000, the fecal samples of 13 patients were analyzed and the obtained data was subjected to a bioinformatics analysis. The bacterial abundance and uniqueness varied in CRC patients alongside differences in bacterial counts between patients. <i>Bacteroides fragilis</i>, <i>Bacteroides vulgatus</i>, <i>Escherichia coli</i>, and <i>Fusobacterium nucleatum</i> were among the pro-cancerous microorganisms found. Concurrently, bacteria linked to CRC progression were detected that have been previously linked to metastasis and recurrence. At the same time, probiotic bacteria such as <i>Bifidobacterium dentium</i>, <i>Bifidobacterium bifidum</i>, and <i>Akkermansia muciniphila</i> increased in abundance after colectomies. Additionally, numerous pathways were deferentially enriched in CRC, which emerged from functional pathways based on bacterial shotgun data. CRC-specific microbiome signatures include an altered bacterial composition. Our research showed that microbial biomarkers could be more usefully employed to explore the link between gut microbiota and CRC using metagenomic techniques in the diagnosis, prognosis, and remission of CRC, thereby opening new avenues for CRC treatment.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"148-160"},"PeriodicalIF":4.8,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What we need to know about the germ-free animal models.","authors":"Fatemeh Aghighi, Mahmoud Salami","doi":"10.3934/microbiol.2024007","DOIUrl":"10.3934/microbiol.2024007","url":null,"abstract":"<p><p>The gut microbiota (GM), as a forgotten organ, refers to the microbial community that resides in the gastrointestinal tract and plays a critical role in a variety of physiological activities in different body organs. The GM affects its targets through neurological, metabolic, immune, and endocrine pathways. The GM is a dynamic system for which exogenous and endogenous factors have negative or positive effects on its density and composition. Since the mid-twentieth century, laboratory animals are known as the major tools for preclinical research; however, each model has its own limitations. So far, two main models have been used to explore the effects of the GM under normal and abnormal conditions: the isolated germ-free and antibiotic-treated models. Both methods have strengths and weaknesses. In many fields of host-microbe interactions, research on these animal models are known as appropriate experimental subjects that enable investigators to directly assess the role of the microbiota on all features of physiology. These animal models present biological model systems to either study outcomes of the absence of microbes, or to verify the effects of colonization with specific and known microbial species. This paper reviews these current approaches and gives advantages and disadvantages of both models.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"107-147"},"PeriodicalIF":4.8,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27.","authors":"Ibtissem Chaib, Scheherazed Dakhmouche-Djekrif, Leila Bennamoun, Tahar Nouadri","doi":"10.3934/microbiol.2024006","DOIUrl":"10.3934/microbiol.2024006","url":null,"abstract":"<p><p>Enzymes are biocatalysts mainly used for their industrial potential in various applications. The present study aims to understand the enzyme production for biotechnological interest from a local yeast strain. From 100 isolates obtained from various biotopes, 78 strains were selected for their enzymatic heritage. Screening of α-amylase, lipase/esterase, and cellulase activities by rapid plate detection methods was carried out and the PO27 yeast was selected for its high capacity to produce α-amylase. In addition, this yeast strain exhibited good lipolytic and esterolytic activities, as well as low cellulase activity. A sequence analysis of the D1/D2 region of the 26S ribosomal RNA (26S rRNA) and a study of morphological characteristics identified the PO27 strain as <i>Geotrichum candidum</i>. The production of α-amylase has been studied in solid medium fermentation using various natural substrates without any supplementation such as olive pomace, potato peels, leftover bread, and mastic cake. <i>G. candidum</i> PO27 showed an improved production of α-amylase with olive pomace, thus reaching approximately 180.71 U/g. To evaluate the ability of this isolate to produce α-amylase in submerged fermentation, multiple concentrations of olive pomace substrate were tested. The best activity of submerged fermentation was statistically compared to the solid-state fermentation result in order to select the appropriate fermentation type. A high significant difference was found to rank the 6% olive pomace medium as the best substrate concentration with 34.395 U/mL of α-amylase activity. This work showed that the new isolate <i>Geotrichum candidum</i> PO27 has a better potential to produce α-amylase at a low cost in solid-state fermentation compared to submerged fermentation. Optimization conditions for PO27 α-amylase production through solid-state fermentation were achieved ‎ using a one factor at a time (OFAT) approach. The findings revealed that a high temperature (60 °C), an acidic pH, malt extract, and soluble starch were the highly significant medium components for enhancing α-‎amylase production. The use of olive pomace waste by <i>Geotrichum candidum</i> PO27 is expected to be effective in producing an industrially useful α-amylase.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"83-106"},"PeriodicalIF":4.8,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annual Report 2023.","authors":"Xu Guo","doi":"10.3934/microbiol.2024004","DOIUrl":"https://doi.org/10.3934/microbiol.2024004","url":null,"abstract":"","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"62-67"},"PeriodicalIF":4.8,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and antimicrobial activity of the tropical ant-derived actinomycetes isolated from Thailand.","authors":"Tuangrat Tunvongvinis, Weeyawat Jaitrong, Yudthana Samung, Somboon Tanasupawat, Wongsakorn Phongsopitanun","doi":"10.3934/microbiol.2024005","DOIUrl":"10.3934/microbiol.2024005","url":null,"abstract":"<p><p>Antibiotic resistance is one of the most important global healthcare challenges and is responsible for the mortality of millions of people worldwide every year. It is a crisis attributed to misuse of antibiotics and a lack of new drug development. Actinomycetes constitute a group of Gram-positive bacteria known for their distinctive high guanine-cytosine (G+C) content in their genomic DNA. These microorganisms are widely recognized for their capability to generate a wide range of secondary metabolites with diverse biological activities. These versatile microorganisms are ubiquitous in diverse ecosystems, including soil, freshwater, marine sediments, and within the bodies of insects. A recent study has demonstrated that social insects, such as ants, host a diverse array of these bacteria. In this study, we involved the isolation and characterization of a total of 72 actinomycete strains obtained from 18 distinct ant species collected from various regions across Thailand. Utilizing 16S rRNA gene analysis, these isolated actinomycetes were classified into four distinct genera: <i>Amycolatopsis</i> (2 isolates), <i>Micromonospora</i> (1 isolate), <i>Nocardia</i> (8 isolates), and <i>Streptomyces</i> (61 isolates). Among the <i>Streptomyces</i> strains, 23 isolates exhibited antimicrobial activity against a panel of Gram-positive bacteria, including <i>Bacillus subtilis</i> ATCC 6633, <i>Staphylococcus epidermidis</i> ATCC 12228, <i>Staphylococcus aureus</i> ATCC 25923, <i>Kocuria rhizophila</i> ATCC 9341, and Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) DMST 20646. Additionally, two isolates displayed antifungal activity against <i>Candida albicans</i> TISTR 5554. Based on 16S rRNA gene sequence similarity studies, these two isolates, ODS25 and ODS28, were demonstrated to be closely related to <i>Streptomyces lusitanus</i> NBRC 13464^T (98.07%) and <i>Streptomyces haliclonae</i> DSM 41970^T (97.28%), respectively. The level of 16S rRNA gene sequence similarity below 98.65% cutoff indicates its potential as a novel actinomycete species. These findings underscore the potential of actinomycetes sourced from ants as a valuable reservoir of novel antimicrobials.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"10 1","pages":"68-82"},"PeriodicalIF":4.8,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}