Unveiling the probiotic potential of L. rhamnosus strain 044AE by genomic and phenotypic characterization.

IF 5.4 Q3 MICROBIOLOGY

AIMS Microbiology Pub Date : 2026-04-08 eCollection Date: 2026-01-01 DOI:10.3934/microbiol.2026007

Aruna Inamdar, Vikash Kumar, Akanksha Chauhan, Yogini Dixit, Namrata Bhingardeve, Kunali Ambavale, Dina Saroj

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Abstract

Probiotics are living microorganisms that improve overall human health by modulating the gut microbiota and enhancing absorption of nutrients and host immunity. Due to the growing demand for probiotics, efforts are being made to search for and characterize new probiotics. Whole genome sequencing has played a significant role in the identification and characterization of many probiotic candidates. In the present study, hybrid assembly (Illumina and Nanopore-based), genomic analyses, and phenotypic evaluations of Lactobacillus rhamnosus 044AE isolated from a dairy sample were carried out to investigate its probiotic potential. The assembled genome of L. rhamnosus 044AE showed maximum homology with L. rhamnosus BIO5326. Downstream analysis of the genome revealed safety, stability, and gut survival features. In the phenotypic assays, L. rhamnosus 044AE exhibited favorable adhesion, aggregation, gut stability (88.96% viability under fasting conditions; 92%-96% viability under fed conditions), antimicrobial, antioxidant, and enzyme activities. Therefore, L. rhamnosus 044AE appears as a potential probiotic candidate for applications in the food, pharmaceutical, and nutraceutical industries.

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通过基因组和表型分析揭示鼠李糖L. rhamnosus菌株044AE的益生菌潜力。

益生菌是一种活的微生物，通过调节肠道菌群和增强营养物质的吸收和宿主免疫力来改善整体人体健康。由于对益生菌的需求不断增长，人们正在努力寻找和表征新的益生菌。全基因组测序在许多候选益生菌的鉴定和表征中发挥了重要作用。在本研究中，通过杂交组装（Illumina和nanopore为基础）、基因组分析和表型评估，对从乳制品样品中分离的鼠李糖乳杆菌044AE进行了研究，以探讨其益生菌潜力。L. rhamnosus 044AE与L. rhamnosus BIO5326的同源性最高。基因组的下游分析揭示了安全性、稳定性和肠道存活特征。在表型分析中，L. rhamnosus 044AE表现出良好的粘附、聚集、肠道稳定性（禁食条件下活力为88.96%，饲喂条件下活力为92%-96%）、抗菌、抗氧化和酶活性。因此，鼠李糖L. rhamnosus 044AE是一种潜在的益生菌候选菌，可用于食品、制药和营养保健行业。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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