AIMS Microbiology最新文献

{"title":"Biofilm production by the multidrug-resistant fungus <i>Candida haemulonii</i> is affected by aspartic peptidase inhibitor.","authors":"Joice Cavalcanti Lima, Lívia de Souza Ramos, Pedro Fernandes Barbosa, Iuri Casemiro Barcellos, Marta Helena Branquinha, André Luis Souza Dos Santos","doi":"10.3934/microbiol.2025012","DOIUrl":"10.3934/microbiol.2025012","url":null,"abstract":"<p><p><i>Candida haemulonii</i> is an emerging, opportunistic, and multidrug-resistant fungal pathogen. Recently, our group reported the ability of <i>C. haemulonii</i> to form biofilm and secrete aspartic-type peptidases (Saps). Herein, we investigated the correlation between Saps production and biofilm formation along <i>C. haemulonii</i> growth in yeast carbon base medium supplemented with albumin (a Sap-inducing condition) and in the presence of the classical Sap inhibitor pepstatin A. Under these conditions, the biofilm biomass increased on a polystyrene surface, reaching its maximum at 96 h, while maximum biofilm viability was detected at 48 h. The release of Saps during biofilm formation showed an inverse trend, with the highest enzymatic activity measured after 24 h. In the presence of pepstatin A, a significant reduction in biofilm parameters (biomass and viability), as well as in albumin consumption by biofilm-forming cells was detected. These findings underscore the importance of Saps during the biofilm development in <i>C. haemulonii</i>.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"228-241"},"PeriodicalIF":2.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid state fermentation: A strategy for wheat bran supplemented corn stover valorization with <i>Pleurotus</i> species.","authors":"Michael Wuaku, Omoanghe S Isikhuemhen, Peter A Dele, Chika C Anotaenwere, Ahmed E Kholif, Oludotun O Adelusi, Joel O Alabi, Deborah O Okedoyin, DeAndrea Gray, Kelechi A Ike, Olatunde A Oderinwale, Jorge A Villarreal-González, Nkese S Udombang, Judith O Enemudo, Nathan Holt, Brandon G Essick, Felicia N Anike, Lauren Mayo, Uchenna Y Anele","doi":"10.3934/microbiol.2025011","DOIUrl":"10.3934/microbiol.2025011","url":null,"abstract":"<p><p>This study compared the potential of three white-rot fungi (<i>Pleurotus</i> spp.) to enhance the nutritional value of corn stover as a feed resource for ruminants. A mixture of shredded corn stover and wheat bran (ratio 9:1) was moisturized (65%), loaded into polypropylene bags, and sterilized at 121 °C for 1 h. Four replicate bags were each inoculated with <i>P. ostreatus</i> (isolates P1 and P3) and <i>P. pulmonarius</i> (isolate P2) and incubated at 25 °C for 0, 2, 4, 6, and 8 weeks. After inoculation and incubation of the corn stover, the resultant substrates and rumen fluid obtained from three ruminally cannulated beef cows were investigated using an <i>in vitro</i> batch culture study, designed as a 3 × 5 factorial with six replicates. Results revealed a significant (p < 0.001) effect on dry matter digestibility (DMD), with the highest DMD observed at 8 weeks for all <i>Pleurotus</i> isolates tested. The best (p < 0.001) performance was seen in corn stover treated with P2 at weeks 6 and 8. Additionally, P1 at 0 and 6 weeks had the lowest ash and highest (p < 0.001) organic matter (OM) concentrations, respectively, compared to P2 at 8 weeks, which had the highest ash and the lowest OM concentrations. The highest (p = 0.011) crude protein (CP) content was recorded in P1 at week 8, while P1 at week 0 had the lowest CP content. Compared to untreated corn stover, higher (p < 0.001) acid detergent fiber digestibility was recorded in corn stover treated with P2 at 8 weeks, while higher (p < 0.001) neutral detergent fiber digestibility was observed in P3 at 2 weeks. <i>Pleurotus</i> strains and incubation periods affected microbial mass production (p < 0.001), with minimal effects on total and individual volatile fatty acids. However, P3 at 2 weeks increased (p = 0.035) acetate and decreased (p = 0.001) propionate proportions. The results indicate that different isolates affected corn stover differently, but in general, all isolates improved the nutritional value of corn stover. <i>P. pulmonarius</i> had the highest DMD and lowest fiber content among the isolates tested and improved energy and nutrient utilization.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"206-227"},"PeriodicalIF":2.7,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome profiling and Actinomycetes isolation from tropical marine sponges.","authors":"Trinset Weeraphan, Chollabuppha Chou, Naphatson Chanthathamrongsiri, Thanchanok Sirirak, Sumaitt Putchakarn, Supakarn Chamni, Wongsakorn Phongsopitanun","doi":"10.3934/microbiol.2025010","DOIUrl":"10.3934/microbiol.2025010","url":null,"abstract":"<p><p>Marine sponges are well-known for their production of bioactive compounds, many of which are synthesized by their associated symbiotic microorganisms. Among these, Actinomycetes are of particular interest due to their ability to produce secondary metabolites with antimicrobial and antitumor activities. We aimed to investigate the bacterial microbiome of tropical marine sponges, with an emphasis on the diversity and distribution of Actinomycetes, employing both culture-dependent and culture-independent approaches. Five sponge samples (PF01-PF05) were collected from Sichang Island, Chonburi Province, Thailand. The bacterial communities were analyzed using 16S rRNA gene sequencing and bioinformatics tools, revealing a significant microbial diversity dominated by Cyanobacteria, Actinomycetota, and Chloroflexi. Notably, PF01 (<i>Penares nux</i>) exhibited the highest microbial diversity, while PF05 (<i>Cacospongia</i> sp.) had the lowest. Actinomycetes, particularly the genus <i>Micromonospora</i>, were successfully isolated from all samples, with PF03 (<i>Ircinia mutans</i>) yielding the highest number of strains. Culture-independent analysis identified a greater proportion of unculturable Actinomycetes compared to those isolated through traditional methods, underscoring the limitations of culture-dependent techniques. This study enhances our understanding of sponge-associated microbial diversity and highlights the potential for isolating Actinomycetes from these sponges for novel drug discovery and other bioprospective applications.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"182-205"},"PeriodicalIF":2.7,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Report of an outbreak of enterovirus disease in a neonatal intensive care unit and a systematic review of the literature.","authors":"Nathan L'Etoile, Lindsay Brim, Susan Coffin, Ericka Hayes","doi":"10.3934/microbiol.2025009","DOIUrl":"10.3934/microbiol.2025009","url":null,"abstract":"<p><p>Neonatal enterovirus infections have the potential to cause devastating illness and death in this vulnerable age group. Existing evidence suggests that the incidence of enteroviral infections in the post-natal period may be higher than previously thought. Because neonates infected with enterovirus are at risk of severe sequelae, and healthcare-associated outbreaks in neonatal settings can occur, enteroviral infection in hospitalized neonates is a serious concern. Thus, it is essential to conduct surveillance for these infections and to deploy robust infection control measures once the virus has been detected in a neonatal care setting. Here, we report an outbreak of enterovirus in a neonatal intensive care unit (NICU) that was rapidly identified and contained, resulting in relatively few cases but requiring temporary closure of the unit. Additionally, we present our review of the literature describing the characteristics of enteroviral outbreaks in NICU and nursery settings to compare published outcomes of outbreaks to those of our outcome.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"167-181"},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brazilian Amazonian microorganisms: A sustainable alternative for plant development.","authors":"Monyck Jeane Dos Santos Lopes, Aline Figueiredo Cardoso, Moacyr Bernardino Dias-Filho, Ely Simone Cajueiro Gurgel, Gisele Barata da Silva","doi":"10.3934/microbiol.2025008","DOIUrl":"10.3934/microbiol.2025008","url":null,"abstract":"<p><p>Plant growth-promoting microorganisms (PGPM) are a sustainable and promising alternative to enhance agricultural production. The Brazilian Amazon, and its mostly unexplored biodiversity, have great potential for identifying and isolating beneficial microorganisms to develop sustainable protocols for plant production with less environmental damage and to meet the increasing demand for food production. Thus, this study aimed to synthesize the findings over the last decade on microorganisms from the Amazon biome that promote plant growth while also addressing the challenges and prospects of this biotechnology. Rhizobacteria (PGPR) and fungi native to the Amazon have been shown to enhance the development of various crops, spanning agriculture and forestry, including palm cultivation and forage crops. The potential of PGPM in the Brazilian Amazon discussed throughout this review highlights the importance of further research in this region. Amazon PGPM is promising for use in the inoculant industry, which would contribute to the agricultural production of diverse crops, reduce costs, minimize the use of chemical inputs, mitigate adverse environmental impacts, and support the conservation of the Amazon biome. Furthermore, the advancement of knowledge in this region holds a great potential, thus offering access to different strains for the formulation of new inoculants that can, in a more sustainable manner, enhance the productivity of various crops, thereby promoting global food security.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"150-166"},"PeriodicalIF":2.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposing of paroxetine and fluoxetine for their antibacterial effects against clinical <i>Pseudomonas aeruginosa</i> isolates in Egypt.","authors":"Kholoud Baraka, Rania Abozahra, Eman Khalaf, Mahmoud Elsayed Bennaya, Sarah M Abdelhamid","doi":"10.3934/microbiol.2025007","DOIUrl":"10.3934/microbiol.2025007","url":null,"abstract":"<p><strong>Background: </strong>Drug repositioning has emerged as a promising strategy for assessing its antimicrobial efficacy in treating infectious diseases.</p><p><strong>Methods: </strong>Seventy-five samples were collected and investigated for the presence of <i>Pseudomonas aeruginosa</i>. Antibiotic resistance, hemolytic activity, twitching motility, and biofilm formation were assessed. <i>lasI</i> and <i>lasR</i> genes were detected using conventional PCR. Minimum inhibitory concentrations of paroxetine, fluoxetine, and levofloxacin were determined by broth micro-dilution. The fractional inhibitory concentration index was calculated to assess the interaction between fluoxetine/levofloxacin and paroxetine/levofloxacin combinations. Half the MIC values of the drugs were selected for inhibitory effect assessment for virulence factors. Antibacterial and healing effects of fluoxetine were investigated on 30 male albino rats using a digital camera, bacterial count, and histological examination.</p><p><strong>Results: </strong>Our 25 <i>P. aeruginosa</i> isolates were highly drug-resistant. 80%, 92%, and 80% of isolates were positive for twitching motility, hemolysis, and biofilm formation, respectively. 92% of isolates were positive for <i>lasI</i> gene and 96% for <i>lasR</i> gene. MICs of fluoxetine and paroxetine ranged from 32 to 512 µg/mL and MICs of levofloxacin ranged from 1 to 256 µg/mL. A synergistic outcome was observed in both combinations. Biofilm formation, twitching motility, and hemolysis were inhibited by paroxetine and fluoxetine in the majority of isolates. Fluoxetine/levofloxacin and paroxetine/levofloxacin combinations inhibited twitching motility, hemolysis, and biofilm formation in all isolates. Enhanced wound healing was observed in rats treated with fluoxetine and levofloxacin, with the fluoxetine/levofloxacin combination group demonstrating the most significant wound-healing effect. Bacterial count decreased in rats treated with levofloxacin, fluoxetine, and the levofloxacin/fluoxetine combination. Histological examination revealed higher wound healing in the levofloxacin-treated group than the fluoxetine group, and the combination treatment group displayed the fastest rate of wound healing.</p><p><strong>Conclusions: </strong>Paroxetine and fluoxetine showed considerable antibacterial inhibitory effects against multi-drug resistant <i>P. aeruginosa</i> isolates. Fluoxetine showed significant improvement in anti-inflammatory effects and wound healing. To the best of our knowledge, this is the first Egyptian study to investigate the repurposing of paroxetine and fluoxetine as antibacterial agents. Further studies are needed to investigate their applicability as antibacterial agents as single agents or in combination with other antibiotics.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"126-149"},"PeriodicalIF":2.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root colonization dynamics of alginate encapsulated rhizobacteria: implications for <i>Arabidopsis thaliana</i> root growth and durum wheat performance.","authors":"Amel Balla, Allaoua Silini, Hafsa Cherif-Silini, Francesca Mapelli, Sara Borin","doi":"10.3934/microbiol.2025006","DOIUrl":"10.3934/microbiol.2025006","url":null,"abstract":"<p><p>Bioencapsulation in alginate capsules offers an interesting opportunity for the efficient delivery of microbial inoculants for agricultural purposes. The present study evaluated the ionic gelation technique to prepare beads loaded with two plant growth-promoting bacteria (PGPB), <i>Bacillus thuringiensis</i> strain B25 and <i>Pantoea agglomerans</i> strain Pa in 1% alginate supplemented with 5mM proline as an osmoprotectant. Capsule morphology, survival rate, encapsulation efficiency, and viability during 24 months of storage as well as the stability of PGP activities were studied. Our results indicate that more than 99% of bacteria were effectively trapped in the alginate beads, which successfully released live bacteria after 60 days of storage at room temperature. A considerable survival of <i>B. thuringiensis</i> B25 throughout the storage period was detected, while the inoculated concentration of 8.72 × 10⁹ (±0.04 ×10⁹) CFU/mL was reduced to 99.9% for <i>P. agglomerans</i> Pa after 24 months of storage. Notably, a higher survival of individually encapsulated bacteria was observed compared to their co-inoculation. The colonization capacity of model plant <i>Arabidopsis thaliana</i> roots by free and encapsulated bacteria was detected by the triphenyltetrazolium chloride test. Moreover, both strains effectively colonized the rhizosphere, rhizoplane, and endosphere of durum wheat plants and exerted a remarkable improvement in plant growth, estimated as a significant increase in the quantities of total proteins, sugars, and chlorophyll pigments, besides roots and shoots length. This study demonstrated that alginate-encapsulated <i>B. thuringiensis</i> B25 and <i>P. agglomerans</i> Pa could be used as inoculants in agriculture, as their encapsulation ensures robust protection, maintenance of viability and PGP activity, and controlled bacterial biostimulant release into the rhizosphere.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"87-125"},"PeriodicalIF":2.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm associated with pigmented areas on a waterproofing coating surface.","authors":"Clotilde Maestri, Ronan L Hébert, Patrick Di Martino","doi":"10.3934/microbiol.2025005","DOIUrl":"10.3934/microbiol.2025005","url":null,"abstract":"<p><p>Waterproofing coatings are composite materials made of different layers with complementary functionalities. They may suffer damage that can modify their aesthetic appearance and/or their functionality. In this study, dark stains appearing on a waterproofing coating of a public swimming pool were mapped and characterized at a macroscopic scale through visual observation and by colorimetric analysis, as well as at a microscopic scale with a digital microscope, a confocal laser scanning microscope, and a scanning electron microscope. Five stains were differentiated macroscopically and characterized using colorimetry and principal component analysis. Microscopic observations showed the presence of microorganisms of varied morphology, some filamentous but mostly unicellular. Biofilms consisting of ovoid fluorescent cells with the morphology of Chlorophyta and unicellular cyanobacteria were particularly abundant. The pigmented stains were located at top coat disorders where microbial colonization and biofilm development were observed. The microscopic observations suggested that physical degradation of the surface of the material would have constituted a prerequisite for colonization by pigmented microorganisms which would have led to the development of macroscopically visible pigmented areas. In this case study, the damage remained superficial and did not alter the watertightness of the material so far.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"74-86"},"PeriodicalIF":2.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-application of biochar and phosphorus increases soil microbial biomass, mycorrhizal colonization, growth, and nutrition of subterranean clover.","authors":"Zakaria M Solaiman, Paul Blackwell, Muhammad Izhar Shafi, Nariman D Salman, Paul Storer, Emre Babur","doi":"10.3934/microbiol.2025004","DOIUrl":"10.3934/microbiol.2025004","url":null,"abstract":"<p><p>Phosphorus (P) plays important roles in the arbuscular mycorrhizal (AM) colonization and rhizobium nodulation processes. Additionally, biochar's positive roles in mycorrhizal colonization and nodulation are articulated. However, the effect of the co-application of biochar and P on AM colonization and rhizobium nodulation was poorly studied. This study investigated the effect of the co-application of wheat straw biochar and P using soil columns on mycorrhizal colonization, nodulation, and the growth of subterranean clover. The soil was amended with wheat straw biochar at 0, 5, and 10 t ha^-1 with different levels of P fertilizer at 0, 5, and 10 kg P ha^-1. These studies showed that adding biochar at 5 t ha^-1 along with mineral P fertilizer increased plant growth, mycorrhizal root colonization and nodulation, and P concentration in plants. In most cases, the increasing trend of the biomass yield was higher when biochar and the P fertilizer were applied together at a higher level (P10). These findings suggested that an increased biochar application rate can increase the subterranean clover growth in soil with either no (P0) or a lower P (P5) fertilizer application. Mycorrhizal colonization could help to improve the P supply and subsequently stimulate the root nodulation of leguminous plants.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"59-73"},"PeriodicalIF":2.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of a <i>Saccharomyces cerevisiae</i> bio-protective strain during cold static clarification on Catarratto wine.","authors":"Enrico Viola, Vincenzo Naselli, Rosario Prestianni, Antonino Pirrone, Antonella Porrello, Filippo Amato, Riccardo Savastano, Antonella Maggio, Micaela Carusi, Venera Seminerio, Valentina Craparo, Azzurra Vella, Davide Alongi, Luca Settanni, Giuseppe Notarbartolo, Nicola Francesca, Antonio Alfonzo","doi":"10.3934/microbiol.2025003","DOIUrl":"10.3934/microbiol.2025003","url":null,"abstract":"<p><p>The study aimed to evaluate the impact of the early addition of a <i>Saccharomyces cerevisiae</i> HD A54 strain before pressing during winemaking. This approach aimed to reduce the dissolved oxygen in the grape must, thus preserving the wine characteristics. Two different treatments were settled: Trial A, where sulphite or other substances were not added during pressing; and Trial B, where a <i>S. cerevisiae</i> strain was added at the pressing stage. The chemical parameters were determined through an enzymatic analyzer, which indicated a faster fructose consumption compared to the glucose in Trial A. The plate counts were measured to monitor the microbial groups during vinification. Both treatments showed regular trends with respect to the <i>Saccharomyces</i> population. Trial B exhibited a higher oxygen consumption compared to the control trial, especially in the early stages of winemaking. This was determined through a dissolved O₂ analysis. Furthermore, Trial B had lower absorbance values at the post-pressing and pre-clarification stages. Both the dissolved oxygen and the absorbance analyses underscored the positive impact of the <i>S. cerevisiae</i> HD A54 strain in protecting against oxidative processes in the grape musts at the pre-fermentative stage. The analysis of volatile organic compounds detected 30 different compounds, including alcohols and esters. Trial B had higher alcohol levels, particularly hydroxyethylbenzene (135.31 mg/L vs. 44.23 mg/L in Trial A). Trial A had almost a four times higher ethyl acetate concentration than Trial B, which is an indicator of oxidation. Interestingly, Trial B showed higher concentrations of 3-methyl-butyl acetate and 2-phenylethyl acetate, which are molecules that correspond to fruity (banana) and floreal (rose) aromas, respectively. Regarding the sensory analysis, Trial B received better scores for the fruity and floral attributes, as well as the overall wine quality.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"11 1","pages":"40-58"},"PeriodicalIF":2.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}