AIMS Microbiology最新文献

{"title":"Biofilm: The invisible culprit in catheter-induced candidemia.","authors":"Meiliyana Wijaya,&nbsp;Ryan Halleyantoro,&nbsp;Jane Florida Kalumpiu","doi":"10.3934/microbiol.2023025","DOIUrl":"https://doi.org/10.3934/microbiol.2023025","url":null,"abstract":"<p><p>Candidemia is the most common form of invasive fungal infection associated with several risk factors, and one of them is the use of medical devices, to which microbial biofilms can attach. Candidemia related to the use of peripheral intravascular and central venous catheters (CVC) is referred to as <i>Candida</i> catheter-related bloodstream infection, with more than 90% being related to CVC usage. The infection is associated with a higher morbidity and mortality rate than nosocomial bacterial infections. <i>Candida</i> spp. can protect themselves from the host immune system and antifungal drugs because of the biofilm structure, which is potentiated by the extracellular matrix (ECM). <i>Candida albicans</i> and <i>Candida parapsilosis</i> are the most pathogenic species often found to form biofilms associated with catheter usage. Biofilm formation of <i>C. albicans</i> includes four mechanisms: attachment, morphogenesis, maturation and dispersion. The biofilms formed between <i>C. albicans</i> and non-albicans spp. differ in ECM structure and composition and are associated with the persistence of colonization to infection for various catheter materials and antifungal resistance. Efforts to combat <i>Candida</i> spp. biofilm formation on catheters are still challenging because not all patients, especially those who are critically ill, can be recommended for catheter removal; also to be considered are the characteristics of the biofilm itself, which readily colonizes the permanent medical devices used. The limited choice and increasing systemic antifungal resistance also make treating it more difficult. Hence, alternative strategies have been developed to manage <i>Candida</i> biofilm. Current options for prevention or therapy in combination with systemic antifungal medications include lock therapy, catheter coating, natural peptide products and photodynamic inactivation.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 3","pages":"467-485"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10184029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobiota community and fungal species response to development stage and fire blight disease in apples.","authors":"Su In Lee,&nbsp;Gyeongjun Cho,&nbsp;Su-Hyeon Kim,&nbsp;Da-Ran Kim,&nbsp;Youn-Sig Kwak","doi":"10.3934/microbiol.2023029","DOIUrl":"https://doi.org/10.3934/microbiol.2023029","url":null,"abstract":"Fire blight disease, caused by the bacterial pathogen Erwinia amylovora, has been a significant concern for over 50 countries worldwide. The efficacy of chemical pesticides currently available for disease control is limited. To address this issue, research is being conducted to explore environmentally friendly control methods, particularly biological control using beneficial microorganisms. However, there is limited research on the apple microbiota community and minimal research has been conducted on fungal communities that may exhibit reliable performance in apple trees. Therefore, our objective was to analyze the fungal communities present in apples at different developmental stages and in different tissues, aiming to identify potential biological control agents for fire blight disease. Our findings indicate that the fungal communities present in apple buds, flowers and leaves play an important role in inhibiting the invasion of E. amylovora. Specifically, we propose GS11 and Lipomyces starkeyi as potential keystone taxa that respond to fire blight disease. These findings provide insights into the continuity and discontinuity of fungal community structure in different developmental stages of apples and offer predictions for potential biological control agents for fire blight disease.","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 3","pages":"554-569"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10184026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>.","authors":"Sunil Kumar,&nbsp;Razique Anwer,&nbsp;Arezki Azzi","doi":"10.3934/microbiol.2023008","DOIUrl":"https://doi.org/10.3934/microbiol.2023008","url":null,"abstract":"<p><p>The emergence and transmission of carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) have been recognized as a major public health concern. Here, we investigated the molecular epidemiology and its correlation with the mechanisms of resistance in CRKP isolates by compiling studies on the molecular epidemiology of CRKP strains worldwide. CRKP is increasing worldwide, with poorly characterized epidemiology in many parts of the world. Biofilm formation, high efflux pump gene expression, elevated rates of resistance, and the presence of different virulence factors in various clones of <i>K. pneumoniae</i> strains are important health concerns in clinical settings. A wide range of techniques has been implemented to study the global epidemiology of CRKP, such as conjugation assays, 16S-23S rDNA, string tests, capsular genotyping, multilocus sequence typing, whole-genome sequencing-based surveys, sequence-based PCR, and pulsed-field gel electrophoresis. There is an urgent need to conduct global epidemiological studies on multidrug-resistant infections of <i>K. pneumoniae</i> across all healthcare institutions worldwide to develop infection prevention and control strategies. In this review, we discuss different typing methods and resistance mechanisms to explore the epidemiology of <i>K. pneumoniae</i> pertaining to human infections.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 1","pages":"112-130"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9238714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of cellulolytic <i>Aspergillus</i> sp. inoculum to improve the quality of Pineapple compost.","authors":"Bambang Irawan,&nbsp;Aandi Saputra,&nbsp;Salman Farisi,&nbsp;Yulianty Yulianty,&nbsp;Sri Wahyuningsih,&nbsp;Noviany Noviany,&nbsp;Yandri Yandri,&nbsp;Sutopo Hadi","doi":"10.3934/microbiol.2023003","DOIUrl":"https://doi.org/10.3934/microbiol.2023003","url":null,"abstract":"<p><p>Pineapple litter has a complex polymer of cellulose, hemicellulose, and lignin, which makes them difficult to decompose. However, pineapple litter has great potential to be a good organic material source for the soil when completely decomposed. The addition of inoculants can facilitate the composting process. This study investigated whether the addition of cellulolytic fungi inoculants to pineapple litters improves the efficiency of the composting processes. The treatments were KP1 = pineapple leaf litter: cow manure (2:1), KP2 = pineapple stem litter: cow manure (2:1), KP3 = pineapple leaf litter: pineapple stem litter: cow manure P1 (leaf litter and 1% inoculum), P2 (stem litter and 1% inoculum), and P3 (leaf + stem litters and 1% inoculum). The result showed that the number of <i>Aspergillus</i> sp. spores on corn media was 5.64 x 10⁷ spores/mL, with viability of 98.58%. <i>Aspergillus</i> sp. inoculum improved the quality of pineapple litter compost, based on the enhanced contents of C, N, P, K, and the C/N ratio, during the seven weeks of composting. Moreover, the best treatment observed in this study was P1. The C/N ratios of compost at P1, P2, and P3 were within the recommended range of organic fertilizer which was 15-25%, with a Carbon/Nitrogen proportion of 11.3%, 11.8%, and 12.4% (P1, P2, and P3), respectively.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 1","pages":"41-54"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9076087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal colonization against <i>Vibrio cholerae</i>: host and microbial resistance mechanisms.","authors":"Abdullahi Yusuf Muhammad,&nbsp;Malik Amonov,&nbsp;Chandrika Murugaiah,&nbsp;Atif Amin Baig,&nbsp;Marina Yusoff","doi":"10.3934/microbiol.2023019","DOIUrl":"https://doi.org/10.3934/microbiol.2023019","url":null,"abstract":"<p><p><i>Vibrio cholerae</i> is a non-invasive enteric pathogen known to cause a major public health problem called cholera. The pathogen inhabits the aquatic environment while outside the human host, it is transmitted into the host easily through ingesting contaminated food and water containing the vibrios, thus causing diarrhoea and vomiting. <i>V. cholerae</i> must resist several layers of colonization resistance mechanisms derived from the host or the gut commensals to successfully survive, grow, and colonize the distal intestinal epithelium, thus causing an infection. The colonization resistance mechanisms derived from the host are not specific to <i>V. cholerae</i> but to all invading pathogens. However, some of the gut commensal-derived colonization resistance may be more specific to the pathogen, making it more challenging to overcome. Consequently, the pathogen has evolved well-coordinated mechanisms that sense and utilize the anti-colonization factors to modulate events that promote its survival and colonization in the gut. This review is aimed at discussing how <i>V. cholerae</i> interacts and resists both host- and microbe-specific colonization resistance mechanisms to cause infection.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 2","pages":"346-374"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotyping and molecular investigation of plasmid-mediated carbapenem resistant clinical <i>Klebsiella pneumoniae</i> isolates in Egypt.","authors":"Kholoud Baraka,&nbsp;Rania Abozahra,&nbsp;Marwa Mohammed Haggag,&nbsp;Sarah M Abdelhamid","doi":"10.3934/microbiol.2023014","DOIUrl":"https://doi.org/10.3934/microbiol.2023014","url":null,"abstract":"<p><p><i>Klebsiella pneumoniae</i> is a multidrug-resistant nosocomial pathogen. Carbapenem resistance is mediated mainly by enzymes carried on transmissible plasmids causing their dissemination among other members of <i>Enterobacteriaceae</i>. This study aimed to molecularly detect carbapenem resistance genes in <i>K. pneumoniae</i> clinical isolates, genotype them using ERIC-PCR, and investigate plasmid transformation of resistant genes by using ERIC-PCR and sequencing.</p><p><strong>Methods: </strong>Antimicrobial resistance of sixty carbapenem-resistant <i>K. pneumoniae</i> strains was evaluated by using the disc diffusion method. Five carbapenemases' genes were amplified by conventional PCR. Genotyping was performed using ERIC-PCR. Gene transformation was performed for the five genes to sensitive isolates. Wild and transformed isolates were genetically investigated using ERIC-PCR and sequencing.</p><p><strong>Results: </strong>Carbapenem resistance in our isolates was associated with high resistance to all tested antibiotics. The 60 <i>K. pneumoniae</i> isolates were divided into 6 resistor types. The prevalence of <i>KPC, IMP, VIM, NDM</i>, and <i>OXA-48</i> genes were 17%, 63%, 93%, 85% and 100%, respectively. Dendrogram analysis showed 57 distinct patterns, arranged in three clusters. The five genes were transformed successfully into sensitive isolates. ERIC profiles of wild and transformed isolates showed cluster A contained all the wild isolates, and cluster B contained all transformed isolates. Genetic sequences of the 5 genes reflected high genetic similarity with the GenBank reference genes before plasmid transformation; however, a distinguishable decrease of genetic similarity was observed after transformation.</p><p><strong>Conclusion: </strong>Plasmid-mediated carbapenem resistance in <i>K. pneumoniae</i> and its dissemination among different strains is a real threat to public health.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 2","pages":"228-244"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of duckweed (<i>Lemnaceae</i>) associated yeasts and their plant growth promoting characteristics.","authors":"Napapohn Kajadpai,&nbsp;Jirameth Angchuan,&nbsp;Pannida Khunnamwong,&nbsp;Nantana Srisuk","doi":"10.3934/microbiol.2023026","DOIUrl":"https://doi.org/10.3934/microbiol.2023026","url":null,"abstract":"<p><p>The diversity of duckweed (<i>Lemnaceae</i>) associated yeasts was studied using a culture-dependent method. A total of 252 yeast strains were isolated from 53 duckweed samples out of the 72 samples collected from 16 provinces in Thailand. Yeast identification was conducted based on the D1/D2 region of the large subunit (LSU) rRNA gene sequence analysis. It revealed that 55.2% and 44.8% yeast species were Ascomycota and Basidiomycota duckweed associated yeasts, respectively. Among all, <i>Papiliotrema laurentii</i>, a basidiomycetous yeast, was found as the most prevalent species showing a relative of frequency and frequency of occurrence of 21.8% and 25%, respectively. In this study, high diversity index values were shown, indicated by the Shannon-Wiener index (<i>H'</i>), Shannon equitability index (<i>E_H</i>) and Simpson diversity index (<i>1-D</i>) values of 3.48, 0.86 and 0.96, respectively. The present results revealed that the yeast community on duckweed had increased species diversity, with evenness among species. Principal coordinate analysis (PCoA) revealed no marked differences in yeast communities among duckweed genera. The species accumulation curve showed that the observed species richness was lower than expected. Investigation of the plant growth promoting traits of the isolated yeast on duckweed revealed that 178 yeast strains produced indole-3-acetic acid (IAA) at levels ranging from 0.08-688.93 mg/L. Moreover, siderophore production and phosphate solubilization were also studied. One hundred and seventy-three yeast strains produced siderophores and exhibited siderophores that showed 0.94-2.55 activity units (AU). One hundred six yeast strains showed phosphate solubilization activity, expressed as solubilization efficiency (SE) units, in the range of 0.32-2.13 SE. This work indicates that duckweed associated yeast is a potential microbial resource that can be used for plant growth promotion.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 3","pages":"486-517"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10130925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of the colistin resistance gene <i>MCR-1</i> in colistin-resistant <i>Klebsiella pneumoniae</i> in Egypt.","authors":"Rania Abozahra,&nbsp;Amal Gaballah,&nbsp;Sarah M Abdelhamid","doi":"10.3934/microbiol.2023011","DOIUrl":"https://doi.org/10.3934/microbiol.2023011","url":null,"abstract":"<p><p><i>Klebsiella pneumoniae</i> is a nosocomial pathogen with high morbidity and mortality rates in hospitalized patients. The emergence of multidrug-resistant <i>K. pneumoniae</i> has become more challenging to treat, with the prevalence of colistin-resistance. Therefore, reliable methods for detecting colistin resistance are required. Many plants' essential oils have antimicrobial activity and have been used to combat multiple antibiotic resistances. This study aimed to investigate the characterization and prevalence of the colistin resistance gene <i>mcr-1</i> in <i>K. pneumoniae</i> in Egypt, evaluate rapid polymyxin NP test, determine the transferability of <i>mcr-1</i> gene, and study the synergistic activity of eugenol combined with colistin against <i>K. pneumoniae</i> isolates. Eighty-two <i>K. pneumonia</i> isolates were collected from different human samples, followed by antibiotic susceptibility testing, rapid polymyxin NP test, and detection of the <i>mcr-1</i> gene and its transfer frequency. Determination of the MICs of colistin alone and in combination with eugenol was performed, then <i>mcr-1</i> gene expression was determined in the presence of eugenol. Thirty-two isolates (39%) were colistin-resistant. Rapid polymyxin NP test failed to detect resistant isolates with MICs below 32 µg/mL. Detection of <i>mcr-1</i> gene was made in 27 (84%) of colistin resistant isolates. The rest isolates possess alteration in the <i>mgrB</i> gene which probably causes colistin resistance. The <i>mcr-1</i> gene was transferred by conjugation to other sensitive isolates. MIC of eugenol ranged from 416 to 1664 µg/mL, and FICI ranged from 0.265 to 0.75. Results also revealed suppression of <i>mcr-1</i> gene expression in the presence of sub MIC of eugenol. Our results demonstrated a high prevalence of <i>mcr-1</i> in Egypt and its ability to transfer to other strains. Difficult determination of colistin-resistant isolates with low values with rapid polymyxin NP test was apparent. Eugenol exerted a synergistic effect with colistin and improved its antimicrobial activity.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 2","pages":"177-194"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9521129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urban endoliths: incidental microbial communities occurring inside concrete.","authors":"Jordan Brown,&nbsp;Corona Chen,&nbsp;Melania Fernández,&nbsp;Deborah Carr","doi":"10.3934/microbiol.2023016","DOIUrl":"https://doi.org/10.3934/microbiol.2023016","url":null,"abstract":"<p><p>Concrete is now a prevalent type of synthetic rock, and its production and usage have major environmental implications. Yet, assessments of ordinary concrete have rarely considered that concrete itself is potential habitat for a globally important microbial guild, the endolithic microbes, which live inside rocks and other mineralized substrates. We sought evidence that many common concrete structures harbor endolithic microbial communities and that these communities vary widely depending on the conditions imposed by the concrete. In Summer 2022, we obtained samples from various concrete structures found throughout Lubbock, Texas, USA and subjected the internal (non-surface) portions of each sample to controlled microbial life detection tests including culture tests, DNA quantifications, DNA amplification tests, and ATP assays. The great preponderance of positive life detection results from our concrete samples suggests that most modern concrete hosts cryptic endolith communities composed of bacteria, sometimes co-occurring with fungi and/or archaea. Moreover, many of these microbes are viable, culturable, and identifiable via genetic analysis. Endolith signatures varied widely across concrete samples; some samples only yielded trace evidence of possibly dormant microbes while other samples contained much more microbial biomass and diversity, on par with some low-biomass soils. Pre-cast masonry units and fragments of poured concrete found underwater generally had the most endolith signatures, suggesting that concrete forms and environmental positioning affect endolithy. Endolith biosignatures were generally greater in less dense and less alkaline concrete samples. So, concrete endolith communities may be as ubiquitous and diverse as the concrete structures they inhabit. We propose further research of concrete endoliths to help clarify the role of modern concrete in our rapidly urbanizing biosphere.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 2","pages":"277-312"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance genes of <i>Escherichia coli</i>, a bacterium of \"One Health\" importance in South Africa: Systematic review and meta-analysis.","authors":"Tsepo Ramatla,&nbsp;Mpho Tawana,&nbsp;Kgaugelo E Lekota,&nbsp;Oriel Thekisoe","doi":"10.3934/microbiol.2023005","DOIUrl":"https://doi.org/10.3934/microbiol.2023005","url":null,"abstract":"<p><p>This is a systematic review and meta-analysis that evaluated the prevalence of <i>Escherichia coli</i> antibiotic-resistant genes (ARGs) in animals, humans, and the environment in South Africa. This study followed Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines to search and use literature published between 1 January 2000 to 12 December 2021, on the prevalence of South African <i>E. coli</i> isolates' ARGs. Articles were downloaded from African Journals Online, PubMed, ScienceDirect, Scopus, and Google Scholar search engines. A random effects meta-analysis was used to estimate the antibiotic-resistant genes of <i>E. coli</i> in animals, humans, and the environment. Out of 10764 published articles, only 23 studies met the inclusion criteria. The obtained results indicated that the pooled prevalence estimates (PPE) of <i>E</i>. <i>coli</i> ARGs was 36.3%, 34.4%, 32.9%, and 28.8% for <i>bla_TEM-M-1</i> , <i>amp</i>C, <i>tet</i>A, and <i>bla</i> _TEM, respectively. Eight ARGs (<i>bla_CTX-M</i> , <i>bla_CTX-M-1</i> , <i>bla_TEM</i> , <i>tet</i>A, <i>tet</i>B, <i>sul</i>1, <i>sul</i>II, and <i>aad</i>A) were detected in humans, animals and the environmental samples. Human <i>E. coli</i> isolate samples harboured 38% of the ARGs. Analyzed data from this study highlights the occurrence of ARGs in <i>E. coli</i> isolates from animals, humans, and environmental samples in South Africa. Therefore, there is a necessity to develop a comprehensive \"One Health\" strategy to assess antibiotics use in order to understand the causes and dynamics of antibiotic resistance development, as such information will enable the formulation of intervention strategies to stop the spread of ARGs in the future.</p>","PeriodicalId":46108,"journal":{"name":"AIMS Microbiology","volume":"9 1","pages":"75-89"},"PeriodicalIF":4.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9076088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}