International Journal of Veterinary Science and Medicine最新文献

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Isolation and Multiple Drug Resistance Patterns of Salmonella Isolates from Selected Dairy Farms in Hawassa Town, Ethiopia 埃塞俄比亚阿瓦萨镇部分奶牛场沙门氏菌分离及多重耐药模式
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-12-30 DOI: 10.13188/2325-4645.1000049
{"title":"Isolation and Multiple Drug Resistance Patterns of Salmonella Isolates from Selected Dairy Farms in Hawassa Town, Ethiopia","authors":"","doi":"10.13188/2325-4645.1000049","DOIUrl":"https://doi.org/10.13188/2325-4645.1000049","url":null,"abstract":"A cross-sectional study was conducted from November 2017 to May 2018 on selected dairy farms in Hawassa town to isolate and assess the in-vitro antibiotic biogram of Salmonella from lactating dairy cows, personnel’s and equipments at farms. A total of 216 samples were collected from selected dairy farms. All samples were processed bacteriologically following standard procedures outlined by ISO 6579: 2002. The overall prevalence of Salmonella was 12.9% (28/216) of the total samples. Out of total, 64.3% (18/28), 10.7% (3/28) and 25% (7/28) were from lactating cows, personnels’, and equipments, respectively. Based on antimicrobial susceptibility testing, all isolates were resistant at least to one or more antimicrobials tested. Accordingly, 96.4% (27/28), 82.1% (23/28) and 75.0% (21/28) isolates showed resistance for oxytetracycline, kanamycin, and nalidixic acid, respectively. Out of all the resistant isolates, 96.4% (27/28) showed multiple antibiotic resistance (resistance to two or more antibiotics) patterns. Multiple antimicrobials resistance was observed in 66.7% (18/27), 7.4% (2/27) and 25.9% (7/27) from lactating cows, personnels’, and equipments, respectively. Thus, awareness creation to the public regarding the public health importance of multiple drug-resistant Salmonella species and the consumption of unpasteurized milk and milk products is important. Fesseha H*, Aliye S, Kifle T and Mathewos M School of Veterinary Medicine, Wolaita Sodo University, Ethiopia *Address for correspondence: Haben Fesseha, School of Veterinary Medicine, Wolaita Sodo University, Ethiopia, PO Box 138, Wolaita Sodo, Southern, Ethiopia; E-mail: tseyon.h@gmail.com Submission: 20-January-2020 Accepted: 21-February-2020 Published: 22February-2020 Copyright: © 2020 Fesseha H Heredia Peralta DT, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Research Article Open Access","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"47 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78401459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Chronic lymphoplasmacytic villonodular proliferative synovitis in a 10-year-old Jack Russell Terrier dog. 10岁杰克罗素梗犬慢性淋巴浆细胞性绒毛结节增生性滑膜炎。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-11-18 DOI: 10.1080/23144599.2020.1842038
Tafara Mapuvire, Erick Kandiwa, Pricilla Mbiri, Alaster Samkange, Oscar Madzingira, Borden Mushonga
{"title":"Chronic lymphoplasmacytic villonodular proliferative synovitis in a 10-year-old Jack Russell Terrier dog.","authors":"Tafara Mapuvire,&nbsp;Erick Kandiwa,&nbsp;Pricilla Mbiri,&nbsp;Alaster Samkange,&nbsp;Oscar Madzingira,&nbsp;Borden Mushonga","doi":"10.1080/23144599.2020.1842038","DOIUrl":"https://doi.org/10.1080/23144599.2020.1842038","url":null,"abstract":"<p><p>We describe a case of chronic lymphoplasmacytic villonodular synovitis (CLPVNS) associated with cranial cruciate ligament (CCL) disease in a 10-year-old spayed Jack Russell Terrier bitch. The bitch was presented to a veterinary clinic with severe, non-weight bearing, acute left hindlimb lameness. The bitch had previously been treated surgically for stifle CCL disease of the same joint, using the lateral fabellar suture (LFS) technique. Since the treatment, the patient had a history of intermittent left hindlimb non-weight bearing lameness that was manageable with nonsteroidal anti-inflammatory drugs (NSAIDs). Palpation and manipulation of the affected stifle elicited severe pain. There were no other clinical or orthopaedic abnormalities. Orthogonal radiographs of the affected stifle revealed moderate degenerative joint disease and osteolytic lesions on the lateral aspect of the lateral femoral condyle and the head of the fibula. A fluid aspirate from this joint was negative for bacterial growth on culture. Cytology results were suspicious for CLPVNS. Exploratory arthrotomy, synovectomy, debridement and lavage of the affected joint were performed. Bone and synovial membrane biopsy samples of the joint were obtained and submitted to a laboratory for a histopathological confirmatory diagnosis. CLPVNS was tentatively diagnosed by cytology, and confirmed by histopathology of biopsy samples. This case report highlights the importance of checking for CLPVNS in dogs with lameness associated with CCL disease, as reports show it to be underreported or misdiagnosed.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"100-105"},"PeriodicalIF":2.2,"publicationDate":"2020-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1842038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38679185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Physiological responses of pigs to preslaughter handling: infrared and thermal imaging applications. 猪对笑前处理的生理反应:红外和热成像应用。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-09-27 DOI: 10.1080/23144599.2020.1821574
Salvador Flores-Peinado, Daniel Mota-Rojas, Isabel Guerrero-Legarreta, Patricia Mora-Medina, Rosy Cruz-Monterrosa, Jocelyn Gómez-Prado, María Guadalupe Hernández, Jesús Cruz-Playas, Julio Martínez-Burnes
{"title":"Physiological responses of pigs to preslaughter handling: infrared and thermal imaging applications.","authors":"Salvador Flores-Peinado,&nbsp;Daniel Mota-Rojas,&nbsp;Isabel Guerrero-Legarreta,&nbsp;Patricia Mora-Medina,&nbsp;Rosy Cruz-Monterrosa,&nbsp;Jocelyn Gómez-Prado,&nbsp;María Guadalupe Hernández,&nbsp;Jesús Cruz-Playas,&nbsp;Julio Martínez-Burnes","doi":"10.1080/23144599.2020.1821574","DOIUrl":"https://doi.org/10.1080/23144599.2020.1821574","url":null,"abstract":"<p><p>Loading, transport, unloading, lairage and stunning are the principle <i>ante-mortem</i> events that generate negative responses associated with stress in pigs. For this reason, it is important to verify the condition of animals throughout the supply chain in order to ensure their welfare and obtain, at the end of the slaughtering process, high-quality carcases and meat. Several studies have identified the indicators and samples that need to be taken into account to properly measure and evaluate the responses that these animals emit to the stressors involved. However, these procedures must be carried out quickly and by non-invasive means so as not to impede the flow of animals through the operations of loading, transport, unloading and lairage. Therefore, the objective of this review article is to analyse the stressful events that pigs experience during these events and discuss the use of the infrared thermography (IRT) as an alternative tool for measuring stress based on temperature changes on the surface of pigs' skin. We argue that infrared thermography can be used as a strategy to improve animal welfare during loading, transport, unloading and lairage by preventing fatigue and deaths, and decreasing negative impacts on meat quality, such as pale, soft and exudative (PSE), or dark, firm and dry (DFD) conditions.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"71-84"},"PeriodicalIF":2.2,"publicationDate":"2020-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1821574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38499052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Morphometric and stereological methods for quantifying the coarse structural parameters of the ruminal tissues in sheep. 定量绵羊瘤胃组织粗结构参数的形态计量学和立体学方法。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-09-08 DOI: 10.1080/23144599.2020.1807817
Andrew Makanya, Ann Nancy Mills-Thompson, James Nguhiu-Mwangi, Jemimah Oduma, Rodi Ojoo
{"title":"Morphometric and stereological methods for quantifying the coarse structural parameters of the ruminal tissues in sheep.","authors":"Andrew Makanya,&nbsp;Ann Nancy Mills-Thompson,&nbsp;James Nguhiu-Mwangi,&nbsp;Jemimah Oduma,&nbsp;Rodi Ojoo","doi":"10.1080/23144599.2020.1807817","DOIUrl":"https://doi.org/10.1080/23144599.2020.1807817","url":null,"abstract":"<p><p>In ruminants, the rumen is the largest and most significant fore-stomach. Stereological analysis of important structural parameters that may be used to assess the functional capacity of the rumen is lacking. In the current investigation, five rams were used to demonstrate the methods for quantifying salient structural parameters related to rumen function. The sheep were euthanized with 20% sodium pentobarbital intravenously, the rumen was dissected out and divided into the various sacs for gross examination, and fixation by total immersion in 10% formalin. Macroscopic ruminal surface area was estimated using the point-associated area method. Volumes of the ruminal tissues were estimated by the volume displacement method, while volume densities of the components of the ruminal wall were estimated by point counting methods. Tissue blocks for histology were obtained by systematic random sampling and processed to obtain vertical sections for surface area and volume estimations. Papillary densities and numbers were estimated from horizontal sections. The volume of ruminal tissue was 536.54 ± 80.52 cm<sup>3</sup>, the macroscopic surface area was 1091 ± 115.75 cm<sup>2</sup> with a papillary packing density of 84.64 ± 10.99 cm<sup>-2</sup>. Average absolute surface area was 4726.74 ± 628.56 cm<sup>2</sup>. The total number of ruminal papillae per rumen was 92,884.91 ± 6216.46. The methods documented here provide the possibility of doing a detailed stereological analysis of ruminal tissue in different experimental or even pathological conditions.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"59-70"},"PeriodicalIF":2.2,"publicationDate":"2020-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1807817","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38803965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A possible case of renal oxalate deposit reported in an African fruit bat (Epomops franqueti). 非洲果蝠肾草酸盐沉积一例报道。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-08-19 DOI: 10.1080/23144599.2020.1807816
O O Aina, M A Olude, F E Olopade, A Balkema-Buschmann, M H Groschup, R Ulrich, J O Olopade
{"title":"A possible case of renal oxalate deposit reported in an African fruit bat (<i>Epomops franqueti</i>).","authors":"O O Aina,&nbsp;M A Olude,&nbsp;F E Olopade,&nbsp;A Balkema-Buschmann,&nbsp;M H Groschup,&nbsp;R Ulrich,&nbsp;J O Olopade","doi":"10.1080/23144599.2020.1807816","DOIUrl":"https://doi.org/10.1080/23144599.2020.1807816","url":null,"abstract":"ABSTRACT We report a possible spontaneous case of oxalate nephrosis in an African fruit bat (Epomops franqueti), incidentally observed in Ibadan, South-West Nigeria, in an anatomical and serological survey of the species. Wild caught bats underwent sedation, intracardial perfusion, necropsy and histopathology. All 15 wild-caught African fruit bats were apparently healthy. However, light microscopy revealed mild oligofocal tubulonephrosis with intraluminal deposition of polarizing crystals interpreted as subclinical oxalate nephrosis in one case. In summary, we suggest a dietary aetiology, based on seasonal availability of high ascorbic acid or oxalate containing fruits. However, exposure to anthropogenic contaminants cannot be completely ruled out.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"56-58"},"PeriodicalIF":2.2,"publicationDate":"2020-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1807816","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38400939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Kick-starting ovarian cyclicity by using dietary glucogenic precursors in post-partum dairy cows: a review. 在产后奶牛中使用膳食糖原性前体启动卵巢周期:综述。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-07-09 DOI: 10.1080/23144599.2020.1773188
W Kaewlamun, B Grimard, C Duvaux-Ponter, A A Ponter
{"title":"Kick-starting ovarian cyclicity by using dietary glucogenic precursors in post-partum dairy cows: a review.","authors":"W Kaewlamun,&nbsp;B Grimard,&nbsp;C Duvaux-Ponter,&nbsp;A A Ponter","doi":"10.1080/23144599.2020.1773188","DOIUrl":"https://doi.org/10.1080/23144599.2020.1773188","url":null,"abstract":"<p><p>The objective of this review is to describe how dietary glucogenic precursors could stimulate ovarian activity in post-partum dairy cows and improve reproductive success. Although the nutrient requirements for the early resumption of ovarian cycles, and for follicle and embryo development are quantitatively small, reproductive success is deteriorated by post-partum negative energy balance. Since very little glucose is absorbed directly from the digestive tract of ruminants one of the targets for nutritional manipulation could be the glucogenic potential of the diet. This could be achieved by giving rumen-resistant starch or mono-propylene glycol. Both these adaptations increase glucose, insulin and insulin-like growth factor-1 plasma concentrations and stimulate ovarian follicle growth.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"39-48"},"PeriodicalIF":2.2,"publicationDate":"2020-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1773188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38400937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Performance of the Parasympathetic Tone Activity (PTA) index to assess the intraoperative nociception using different premedication drugs in anaesthetised dogs. 用不同用药前药物评价麻醉犬术中伤害感受的副交感神经张力活动(PTA)指数。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-07-07 DOI: 10.1080/23144599.2020.1783090
Christelle Mansour, Nour El Hachem, Patrick Jamous, Georges Saade, Emmanuel Boselli, Bernard Allaouchiche, Jeanne-Marie Bonnet, Stéphane Junot, Rana Chaaya
{"title":"Performance of the Parasympathetic Tone Activity (PTA) index to assess the intraoperative nociception using different premedication drugs in anaesthetised dogs.","authors":"Christelle Mansour,&nbsp;Nour El Hachem,&nbsp;Patrick Jamous,&nbsp;Georges Saade,&nbsp;Emmanuel Boselli,&nbsp;Bernard Allaouchiche,&nbsp;Jeanne-Marie Bonnet,&nbsp;Stéphane Junot,&nbsp;Rana Chaaya","doi":"10.1080/23144599.2020.1783090","DOIUrl":"https://doi.org/10.1080/23144599.2020.1783090","url":null,"abstract":"<p><p>The dynamic variations of the Parasympathetic Tone Activity (PTA) index were evaluated to assess nociception in dogs undergoing anaesthetic protocols with different premedication drugs. Sixty-six dogs, divided into three groups of 22 dogs each, were given different premedication drugs \"morphine\" (0.2 mg/kg), \"morphine + medetomidine (5 μg/kg)\", \"morphine + acepromazine (0.03 mg/kg)\", then similarly induced and maintained under general anaesthesia. The PTA, HR (heart rate) and MAP (mean arterial pressure) were assessed at S (steady-state), Cut (cutaneous incision), PTA<sub>E</sub> (PTA Event, assessed 1 min before a significant PTA decrease), HDR (Haemodynamic reaction, assessed 5 min before an increase of ≥ 20% in HR and/or MAP). For each group, the dynamic variation of PTA (ΔPTA) was calculated and a Receiver Operating Characteristics (ROC) curve was performed to detect if any of the premedication drugs may alter the performance of PTA index to predict intraoperative haemodynamic reactions. In all groups, a haemodynamic reaction was detected after Cut, PTA<sub>E</sub> and HDR and was preceded with a significant decrease of PTA, except for \"morphine + medetomidine\" group which showed a significant drop of PTA only at PTA<sub>E</sub> and HDR. The ΔPTA showed a fair performance in all groups (a mean [95% CI] AUC of 0.73 [0.62-0.82], 0.70 [0.59-0.79] and 0.71 [0.59-0.80] respectively in morphine, morphine + ACP and morphine + medetomidine). Although ΔPTA was rather altered by the premedication drug, it was able to predict, to a certain extent, haemodynamic reactions in all groups.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"49-55"},"PeriodicalIF":2.2,"publicationDate":"2020-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1783090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38400938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Pharmacokinetics and tissue residue of enrofloxacin in healthy, Eimeria-infected broiler chickens and those pre-treated with amprolium and toltrazuril. 恩诺沙星在感染艾美耳菌的健康肉鸡及经氨丙铵和托曲祖利预处理的肉鸡体内的药动学和组织残留。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-06-01 DOI: 10.1080/23144599.2020.1765720
M Atef, H A El-Banna, H Y Elzorba, A M Soliman
{"title":"Pharmacokinetics and tissue residue of enrofloxacin in healthy, <i>Eimeria</i>-infected broiler chickens and those pre-treated with amprolium and toltrazuril.","authors":"M Atef,&nbsp;H A El-Banna,&nbsp;H Y Elzorba,&nbsp;A M Soliman","doi":"10.1080/23144599.2020.1765720","DOIUrl":"https://doi.org/10.1080/23144599.2020.1765720","url":null,"abstract":"<p><p>The pharmacokinetics of enrofloxacin was compared in healthy chickens, <i>Eimeria</i> infected chickens and in <i>Eimeria</i> infected chickens pre-treated with amprolium or toltrazuril following a single IV and oral administration at dose 10 mg/kg. The blood samples were taken after administration at different time intervals (5 min to 24 hours) to determine the pharmacokinetic parameters of enrofloxacin. The different concentrations of enrofloxacin were determined by using HPLC assay method. Serum concentrations versus time were analysed by a non-compartmental method. The results explored a significant decrease in serum concentrations of enrofloxacin at different time intervals and a significant change in pharmacokinetic profiles in <i>Eimeria</i> infected chickens compared with those values in healthy chickens whereas, amprolium improves these values. Toltrazuril leads to a significant decrease in enrofloxacin concentrations compared with infected non-treated chickens. Multiple-dose study revealed a longer withdrawal period of enrofloxacin in infected non-treated and infected chickens pre-treated with amprolium compared with the healthy group.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"31-38"},"PeriodicalIF":2.2,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1765720","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38377111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Detection of the epidemic of the H3N8 subtype of the equine influenza virus in large-scale donkey farms. 大型驴场马流感病毒H3N8亚型流行的检测。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-03-31 eCollection Date: 2020-01-01 DOI: 10.1080/23144599.2020.1739844
Yu Yongfeng, Sun Xiaobo, Xia Nan, Zhang Jingwen, Liu Wenqiang
{"title":"Detection of the epidemic of the H3N8 subtype of the equine influenza virus in large-scale donkey farms.","authors":"Yu Yongfeng,&nbsp;Sun Xiaobo,&nbsp;Xia Nan,&nbsp;Zhang Jingwen,&nbsp;Liu Wenqiang","doi":"10.1080/23144599.2020.1739844","DOIUrl":"https://doi.org/10.1080/23144599.2020.1739844","url":null,"abstract":"<p><p>To monitor the occurrence of equine influenza in large-scale donkey farms in Liaocheng City, Shandong Province, serological investigation and sequence analysis of HA/M protein gene of equine influenza virus (EIV) were carried out. Samples (n = 65) of the lung and nasal swab were collected in six different large-scale donkey farms and detected with RT-PCR for HA and M protein gene. The homology and evolution of HA and M genes were analysed with known sequences. Antibody titres of serum samples (n = 120, unvaccinated) level was determined by the HI test. The average seropositive rate was 32.5% (39/120) with great diversity among different populations. The positive rate of EIV HA/M protein gene was 21.5% (14/65) by RT-PCR. The equine influenza H3N8 virus was confirmed by gene sequencing, and the homology of the sequence was 99.77% with isolates from Northeast China (equine/heilongjiang/1/2010), consistent with the input of donkeys. This suggested that EIV has become an important threat to large-scale donkey farms in Liaocheng and threats from the input area must be vigilant.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"26-30"},"PeriodicalIF":2.2,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2020.1739844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37878404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Haemato-biochemical changes and prevalence of parasitic infections of indigenous chicken sold in markets of Kiambu County, Kenya. 肯尼亚基安布县市场上出售的本地鸡的血液生化变化和寄生虫感染流行率。
IF 2.2
International Journal of Veterinary Science and Medicine Pub Date : 2020-02-13 eCollection Date: 2020-01-01 DOI: 10.1080/23144599.2019.1708577
Peninah Wamboi, Robert M Waruiru, Paul G Mbuthia, James M Nguhiu, Lilly C Bebora
{"title":"Haemato-biochemical changes and prevalence of parasitic infections of indigenous chicken sold in markets of Kiambu County, Kenya.","authors":"Peninah Wamboi,&nbsp;Robert M Waruiru,&nbsp;Paul G Mbuthia,&nbsp;James M Nguhiu,&nbsp;Lilly C Bebora","doi":"10.1080/23144599.2019.1708577","DOIUrl":"https://doi.org/10.1080/23144599.2019.1708577","url":null,"abstract":"<p><p>This study aimed at determining parasitic prevalence and probable haemato-biochemical changes that may occur from parasitic infections in marketed indigenous chickens in Kiambu County, Kenya. Thirty adult chickens were purchased and examined for ectoparasites, haemoparasites and haemato-biochemical changes. Post mortem was conducted to recover gastro-intestinal parasites and fecal samples taken for egg/oocyst counts. Forty-seven percent (14/30) of chickens examined were in poor body condition, 43% (13/30) in fair and 10% (3/30) in good body condition. Ectoparasites infection prevalence was 66.7% (20/30). Four haemoparasites were isolated. Overall helminths prevalence was 86.6% (26/30), nematodes at 76.7% (23/30) and cestodes at 40% (12/30). After processing fecal samples, 30% (9/30) were positive for helminth eggs and 30% (9/30) had coccidial oocysts. Relative to normal values, total erythrocyte count was low and total leucocyte count with band cells high. Mean haematocrit and heterophil values were high (p=0.0005; p=0.0061). Mean lymphocyte count was low (p=0.0128) in chickens with ectoparasitic infestation. Eosinophils increased significantly (p=0.0363) although mean erythrocytes counts decreased (p=0.0176), in chickens with gastrointestinal parasites. Creatine phosphokinase and blood glucose levels were high, serum protein and albumin levels were low. Blood glucose level decreased significantly (p=0.0239) and total plasma protein increased (p=0.045) in chickens with Haemoproteus spp. infection. The study showed, ecto- and endo-parasites are prevalent and may contribute to alteration of haemato-biochemical parameters of sub-clinically infected marketed indigenous chickens. These results are expected to contribute towards and encourage usage of clinico-pathological parameter testing as a measure of poultry health status for enhanced poultry disease diagnoses.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":" ","pages":"18-25"},"PeriodicalIF":2.2,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2019.1708577","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37702452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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