International Journal of Veterinary Science and Medicine最新文献

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Prevalence of Cryptosporidium parvum, Giardia duodenalis and Eimeria spp. in diarrhoeic suckling calves from north-western Spain and analysis of their interactions. 西班牙西北部腹泻哺乳犊牛小隐孢子虫、十二指肠贾第鞭毛虫和艾美耳虫的流行及其相互作用分析。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2025-01-09 eCollection Date: 2025-01-01 DOI: 10.1080/23144599.2024.2447172
Cynthia López-Novo, Seila Couso-Pérez, Alberto Prieto, Jose Manuel Díaz-Cao, David García-Dios, Gonzalo López-Lorenzo, Susana Remesar, Elvira Ares-Mazás, Ceferino López, Patrocinio Morrondo, Hipólito Gómez-Couso, Pablo Díaz
{"title":"Prevalence of <i>Cryptosporidium parvum</i>, <i>Giardia duodenalis</i> and <i>Eimeria</i> spp. in diarrhoeic suckling calves from north-western Spain and analysis of their interactions.","authors":"Cynthia López-Novo, Seila Couso-Pérez, Alberto Prieto, Jose Manuel Díaz-Cao, David García-Dios, Gonzalo López-Lorenzo, Susana Remesar, Elvira Ares-Mazás, Ceferino López, Patrocinio Morrondo, Hipólito Gómez-Couso, Pablo Díaz","doi":"10.1080/23144599.2024.2447172","DOIUrl":"10.1080/23144599.2024.2447172","url":null,"abstract":"<p><p>Although <i>Cryptosporidium parvum</i>, <i>Giardia duodenalis</i> and some <i>Eimeria</i> species are frequently involved in neonatal calf diarrhoea (NCD), detailed studies on their interactions are scarce. Therefore, a cross-sectional study including faecal samples from 404 diarrhoeic calves aged 0-30 days was performed. <i>Cryptosporidium</i> oocysts and <i>G. duodenalis</i> cysts were detected by immunofluorescence antibody test and positive samples were molecularly characterized. <i>Eimeria</i> oocysts were microscopically detected using the modified McMaster technique and morphometric species identification was performed. <i>Cryptosporidium</i> infections (53.7%) predominated over those by <i>G. duodenalis</i> (12.4%) and <i>Eimeria</i> spp. (6.9%). Three <i>Cryptosporidium</i> species were identified: <i>C. parvum</i> (98.0%), <i>C. bovis</i> (1.0%) and <i>C. ryanae</i> (1.0%). Eleven <i>C. parvum</i> zoonotic subtypes were detected, with IIaA15G2R1 as the most common; only <i>G</i>. <i>duodenalis</i> assemblage E was identified. Moreover, 10 <i>Eimeria</i> species were found, being <i>Eimeria ellipsoidalis</i> (96.4%), <i>Eimeria bovis</i> (85.7%), and <i>Eimeria zuernii</i> (71.4%) predominant. A significant positive association between <i>G. duodenalis</i> and <i>Eimeria</i> spp. was detected as well as a negative association between <i>C. parvum</i> and <i>Eimeria</i> spp. Our results showed that <i>C. parvum</i> wasprevalent in diarrhoeic calves under 21 days of life; <i>Eimeria</i> spp. and <i>G. duodenalis</i> were especially common in animals in their fourth week of life. Concurrent infections increased with age. Moreover, the study also revealed potential public health risks since a noticeable percentage of animals were infected with <i>C. parvum</i> zoonotic subtypes. Further studies are needed for determining the role of these parasites in co-infections with other enteropathogens and their implications in the pathogenicity of NCD.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monocephalus dipygus in a 4-month-old mixed-breed puppy: clinical, radiographic, ultrasonographic, karyotyping and surgical intervention. 一只 4 个月大混血幼犬的单脑双畸形:临床、放射学、超声波、核型分析和手术干预。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-11-10 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2418773
Lorena Elizabeth Chalco-Torres, Ana Elizabeth Guerrero López, Mauro Nirchio Tursellino
{"title":"Monocephalus dipygus in a 4-month-old mixed-breed puppy: clinical, radiographic, ultrasonographic, karyotyping and surgical intervention.","authors":"Lorena Elizabeth Chalco-Torres, Ana Elizabeth Guerrero López, Mauro Nirchio Tursellino","doi":"10.1080/23144599.2024.2418773","DOIUrl":"https://doi.org/10.1080/23144599.2024.2418773","url":null,"abstract":"<p><p>Congenital anomalies are rare in veterinary medicine, and their aetiology, development, clinical presentation, and management remain poorly understood. This study documents a rare case of multiple congenital anomalies occurring simultaneously in a single individual. We present a comprehensive analysis of a 4-month-old mixed-breed puppy diagnosed with caudal duplication (monocephalus dipygus), integrating clinical, radiographic, ultrasonographic, cytogenetic findings, and surgical intervention. The puppy exhibited several external abnormalities, including a supernumerary limb, a single tail, two penises, and two distinct scrotal sacs. Radiographic examination revealed additional metatarsal and phalangeal bones in the supernumerary limb. Ultrasonography and exploratory laparotomy further identified two bladders, a single prostate, and bifurcated ureters, each connecting to a kidney. Cytogenetic analysis was conducted to investigate potential chromosomal abnormalities underlying these congenital anomalies, as we hypothesized that these malformations might be linked to chromosomal aberrations. The successful surgical removal of the supernumerary limb significantly improved the dog's mobility. Notably, since each bladder was independently connected to a kidney, surgical intervention in the genitourinary system was avoided to prevent potential impairment of urinary function. This case highlights the complexity of congenital anomalies in canines and emphasizes the importance of a multidisciplinary approach to their diagnosis and management.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"148-156"},"PeriodicalIF":2.8,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual effects of ipecac alkaloids with potent antiviral activity against foot-and-mouth disease virus as replicase inhibitors and direct virucides. 作为复制酶抑制剂和直接杀病毒剂,对口蹄疫病毒具有强效抗病毒活性的依百加生物碱具有双重作用。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-10-01 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2408189
Achiraya Pantanam, Natjira Mana, Ploypailin Semkum, Varanya Lueangaramkul, Nantawan Phecharat, Porntippa Lekcharoensuk, Sirin Theerawatanasirikul
{"title":"Dual effects of ipecac alkaloids with potent antiviral activity against foot-and-mouth disease virus as replicase inhibitors and direct virucides.","authors":"Achiraya Pantanam, Natjira Mana, Ploypailin Semkum, Varanya Lueangaramkul, Nantawan Phecharat, Porntippa Lekcharoensuk, Sirin Theerawatanasirikul","doi":"10.1080/23144599.2024.2408189","DOIUrl":"10.1080/23144599.2024.2408189","url":null,"abstract":"<p><p>Foot-and-Mouth Disease (FMD) is a contagious, blistering disease caused by the Foot-and-Mouth Disease virus (FMDV), which affects livestock globally. Currently, no commercial antiviral agent is available for effective disease control. This study investigated the antiviral potential of natural-derived alkaloids against FMDV in BHK-21 cells. Twelve alkaloids were assessed for their antiviral activities at various stages of FMDV infection, including pre-viral entry, post-viral entry, and prophylactic assays, as well as attachment and penetration assays by evaluating cytopathic effect reduction and directed-virucidal effects. The results showed that ipecac alkaloids, cephaeline (CPL) and emetine (EMT), exhibited dual effects with robust antiviral efficacy by reducing cytopathic effect and inhibiting FMDV replication in a dose-dependent manner. Evaluation through immunoperoxidase monolayer assay and RT-PCR indicated effectiveness at post-viral entry stage, with sub-micromolar EC<sub>50</sub> values for CPL and EMT at 0.05 and 0.24 µM, respectively, and high selective indices. Prophylactic effects prevented infection with EC<sub>50</sub> values of 0.23 and 0.64 µM, respectively. Directed-virucidal effects demonstrated significant reduction of extracellular FMDV, with CPL exhibiting a dose-dependent effect. Furthermore, the replicase (3Dpol) inhibition activity was identified using the FMDV minigenome assay, which revealed strong inhibition with IC<sub>50</sub> values of 0.15 µM for CPL and 4.20 µM for EMT, consistent with the decreased negative-stranded RNA production. Molecular docking confirmed the interaction of CPL and EMT with residues in the active site of FMDV 3Dpol. In conclusion, CPL and EMT exhibited promising efficacy through their dual effects and provide an alternative approach for controlling FMD in livestock.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"134-147"},"PeriodicalIF":2.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of Fasciola gigantica in freshwater snail Radix (Lymnaea) spp. In the highly parasite-prevalent area of Nakhon Ratchasima Province, Thailand. 泰国呵叻府淡水蜗牛(Lymnaea)属寄生虫高发区的巨尾蜗牛(Fasciola gigantica)调查。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-09-10 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2396700
Pongsakorn Martviset, Pathanin Chantree, Amornrat Geadkaew-Krenc, Pantip Piyatadsananon, Ruttiroj Jirojwong, Chompunoot Wangboon, Mantana Jamklang, Sirilak Chumkiew, Rawipreeya Poomkhokrak, Nanthawat Kosa, Salisa Chaimon, Bumpenporn Sanannam, Rudi Grams, Wansika Phadungsil
{"title":"Investigation of <i>Fasciola gigantica</i> in freshwater snail <i>Radix</i> (<i>Lymnaea</i>) spp. In the highly parasite-prevalent area of Nakhon Ratchasima Province, Thailand.","authors":"Pongsakorn Martviset, Pathanin Chantree, Amornrat Geadkaew-Krenc, Pantip Piyatadsananon, Ruttiroj Jirojwong, Chompunoot Wangboon, Mantana Jamklang, Sirilak Chumkiew, Rawipreeya Poomkhokrak, Nanthawat Kosa, Salisa Chaimon, Bumpenporn Sanannam, Rudi Grams, Wansika Phadungsil","doi":"10.1080/23144599.2024.2396700","DOIUrl":"https://doi.org/10.1080/23144599.2024.2396700","url":null,"abstract":"<p><p>This study investigates the distribution of the <i>Lymnaea</i> (<i>Radix</i>) spp. in Pak Chong district, Nakhon Ratchasima province, northeast Thailand, where a vast cattle farming area is located and has a high prevalence of <i>Fasciola</i> spp. in the cattle. By random selection, 1,414 snails were collected from the natural and man-made ponds. The snails were recorded for morphology and processed for DNA isolation. The snail species were investigated by conventional PCR using a 16S rDNA-specific primer. The result demonstrated that all collected snails were <i>R</i>. (<i>L</i>.) <i>rubiginosa</i>. Moreover, the infection of <i>Fasciola</i> gigantica in the snails was investigated by PCR using a cytochrome c oxidase I (COX1)-specific primer. The results illustrated that the overall prevalence was 22.5% (318/1414), with the highest prevalence in the Nong Sa Rai subdistrict at 73.6% (81/110), which is the highest prevalence of <i>Fasciola gigantica</i> in the snail host that has ever been reported. The lowest prevalence existed in the Pong Ta Long subdistrict at 3.7% (4/109). Our results corresponded to the previous report on the <i>Fasciola</i> spp. infection in the cattle from this area, and the geographical analysis revealed that the most suspected factor would be the earth dam located in these subdistricts, where many animals live freely during the day. Our findings could be helpful for further parasite control and could trigger the study of the biology and associated factors in the future.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"125-133"},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microstructural architecture of the bony scutes, spine, and rays of the bony fins in the common pleco (Hypostomus plecostomus). 普通褶鱼(Hypostomus plecostomus)骨鳞、骨刺和骨鳍的微观结构。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-09-04 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2374201
Hanan H Abd-Elhafeez, Diaa Massoud, Mohammed S Mahmoud, Nada Abdellah, Abdallah S Salah, Nor-Elhoda Mohamed, Mennatallah Ali Abdelrhman Sayed, Mohamed Shaalan, Catrin S Rutland, Alaa Sayed Abu-ELhamed, Soha A Soliman, Fatma El-Zahraa A Mustafa
{"title":"Microstructural architecture of the bony scutes, spine, and rays of the bony fins in the common pleco <i>(Hypostomus plecostomus)</i>.","authors":"Hanan H Abd-Elhafeez, Diaa Massoud, Mohammed S Mahmoud, Nada Abdellah, Abdallah S Salah, Nor-Elhoda Mohamed, Mennatallah Ali Abdelrhman Sayed, Mohamed Shaalan, Catrin S Rutland, Alaa Sayed Abu-ELhamed, Soha A Soliman, Fatma El-Zahraa A Mustafa","doi":"10.1080/23144599.2024.2374201","DOIUrl":"10.1080/23144599.2024.2374201","url":null,"abstract":"<p><p>Studying scute and fin morphology are advantageous approaches for phylogenetic identification and provide information on biological linkages and evolutionary history that are essential for deciphering the fossil record. Despite this, no prior research has precisely characterized the histological structures of scutes in the common pleco. Therefore, this research investigated the microstructure and organization of bone tissue within the dermal skeleton, including the scutes and fins, in the common pleco, using light microscopy, stereomicroscopy, and scanning electron microscopy. The dermal scutes were organized in a pentagonal shape with denticular coverage and were obliquely aligned with the caudal portion pointing dorsally. The dermal scutes consisted of three distinct portions: the central, preterminal, and terminal portions. Each portion comprised three layers: a superficial bony plate, a basal bony plate, and a mid-plate. Both the superficial and basal bony plates were composed of lamellar bone and lamellar zonal bone, whilst the mid-plate consisted of secondary osteons and woven bone. In the terminal portion, the superficial and basal bony plates became thinner. The pectoral fin consists of spines and rays composed of lepidotrichium (two symmetrical hemi-rays). The spine contained centrifugal and centripetal lamellar and trabecular bones. A centripetal fibrous bone was implanted between the lamellar bones. Besides being oriented in a V shape, the hemi-rays were also composed of thin centrifugal and centripetal lamellar bones and trabecular bones. A fibrous bone was identified between the centrifugal and centripetal bones. The trabecular bone and lamellar bone were made up of bone spicules.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"101-124"},"PeriodicalIF":2.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seroprevalence and molecular detection of Brucella infection in livestock in the United Arab Emirates. 阿拉伯联合酋长国牲畜布鲁氏菌感染的血清流行率和分子检测。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-08-23 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2384015
Gobena Ameni, Aboma Zewude, Berecha Bayissa, Ibrahim Abdalla Alfaki, Abdallah A Albizreh, Naeema Alhosani, Khaja Mohteshamuddin, Berhanu Adenew Degefa, Mohamed Elfatih Hamad, Meera Saeed Alkalbani, Mohamed Moustafa Abdelhalim, Assem Sobhi Abdelazim, Rafeek Aroul Koliyan, Kaltham Kayaf, Mervat Mari Al Nuaimat, Robert Barigye, Arve Lee Willingham, Markos Tibbo, Bedaso Mammo Edo, Teshale Sori, Yassir Mohammed Eltahir
{"title":"Seroprevalence and molecular detection of <i>Brucella</i> infection in livestock in the United Arab Emirates.","authors":"Gobena Ameni, Aboma Zewude, Berecha Bayissa, Ibrahim Abdalla Alfaki, Abdallah A Albizreh, Naeema Alhosani, Khaja Mohteshamuddin, Berhanu Adenew Degefa, Mohamed Elfatih Hamad, Meera Saeed Alkalbani, Mohamed Moustafa Abdelhalim, Assem Sobhi Abdelazim, Rafeek Aroul Koliyan, Kaltham Kayaf, Mervat Mari Al Nuaimat, Robert Barigye, Arve Lee Willingham, Markos Tibbo, Bedaso Mammo Edo, Teshale Sori, Yassir Mohammed Eltahir","doi":"10.1080/23144599.2024.2384015","DOIUrl":"10.1080/23144599.2024.2384015","url":null,"abstract":"<p><p>Small ruminants and camels are important livestock species in the United Arab Emirates (UAE), although <i>Brucella</i> infection can limit their productivity. This study aimed to investigate the seroprevalence of <i>Brucella</i> infection and its associated risk factors in small ruminants and camels in the Emirate of Abu Dhabi. Additionally, seropositive animals were tested for the DNA of <i>Brucella</i>. Multispecies competitive enzyme-linked immunosorbent assay (c-ELISA) and multispecies indirect (i-ELISA) were used to test 3,086 animals from 2022 to 2023. <i>Brucella</i> cell surface 31 kDa protein (bcsp31) gene-based real-time polymerase chain reaction (q-PCR) was used to detect <i>Brucella</i> DNA. Multivariate logistic regression was used to assess the association between seroprevalence and potential risk factors. The overall seroprevalences of <i>Brucella</i> infection were 1.7% (95% confidence interval [CI], 1.2%-2.2%) and 5.8% (95% CI, 5.0%-6.7%) based on serial and parallel testing, respectively. The DNA of <i>Brucella</i> was detected in 13 of the 51 seropositive animals. The overall seroprevalence of <i>Brucella</i> infection was associated with the region, type of animal holding, species, and age of the animals. In conclusion, this study documented <i>Brucella</i> infection in small ruminants and camels in the Emirate of Abu Dhabi, warranting necessary intervention strategies to eliminate <i>Brucella</i> infections in livestock populations.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"91-100"},"PeriodicalIF":2.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11346334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of induced pluripotent stem cells from the Asian bats. 从亚洲蝙蝠体内生成诱导多能干细胞。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-08-12 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2384835
Younsu Lee, Okjae Koo, Islam M Saadeldin
{"title":"Generation of induced pluripotent stem cells from the Asian bats.","authors":"Younsu Lee, Okjae Koo, Islam M Saadeldin","doi":"10.1080/23144599.2024.2384835","DOIUrl":"10.1080/23144599.2024.2384835","url":null,"abstract":"<p><p>Preservation of native Korean bats is crucial for maintaining ecological balance, as they play a vital role in insect control, pollination, and seed dispersal within their ecosystems. The present study details the establishment of bat induced pluripotent stem cells (BatiPSCs) from two Asian and Korean bats (<i>Hypsugo alaschanicus</i> and <i>Pipistrellus abramus</i>) using the Sendai Reprogramming Kit. Colonies of BatiPSCs, exhibiting distinctive features, were manually selected and expanded following successful transfection. Characterization of BatiPSCs revealed the expression of pluripotency markers, such as Octamer-binding transcription factor 4 (Oct4), SRY (sex-determining region Y)-box 2 and Nanog, with notably increased Oct4 levels and reduced Myc proto-oncogene expression compared with those noted in other induced pluripotent stem cell sources. BatiPSCs displayed positive staining for alkaline phosphatase and demonstrated the ability to form embryoid bodies, while also inducing teratomas in non-immune nude mice. Additionally, green fluorescent protein (GFP)-expressing BatiPSCs were generated and used for chimeric mouse production, with slight GFP signals detected in the neck region of the resulting mouse foetuses. These findings demonstrate the successful generation and characterization of BatiPSCs, emphasizing their potential applications in chimeric animal models, and the protection of endangered bat species.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"81-90"},"PeriodicalIF":2.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate. 保持马血小板裂解液体外生物活性的冷冻干燥方案和方法。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-08-07 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2380586
Chiara Bernardini, Noemi Romagnoli, Isabelle Casalini, Maria Elena Turba, Alessandro Spadari, Monica Forni, Fabio Gentilini
{"title":"Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate.","authors":"Chiara Bernardini, Noemi Romagnoli, Isabelle Casalini, Maria Elena Turba, Alessandro Spadari, Monica Forni, Fabio Gentilini","doi":"10.1080/23144599.2024.2380586","DOIUrl":"10.1080/23144599.2024.2380586","url":null,"abstract":"<p><p>Platelet lysate, derived from platelets, are valuable biological products rich in bioactive molecules. Their use promotes tissue healing and modulates inflammation. However, maintaining the stability and bioactivity of platelet lysate is challenging since they degrade rapidly at room temperature. This study focused on the possibility to confer enhanced stability to freeze-dried equine platelet lysate as an alternative to platelet-rich plasma (PRP). Platelet lysate (PL) was derived from PRP and freeze-dried either as such or using various adjuvants. Primary cell cultures of porcine Vascular Wall-Mesenchymal Stem Cells were treated with different PL formulations, and cell viability was assessed using an MTT assay. Overall, the addition of PL significantly improved cell viability as compared to controls without growth factor supplementation or with foetal bovine serum. Notably, the freeze-drying process maintained the effectiveness of the PL for at least a week. Furthermore, the study revealed that varying the horse as the source of PL could yield varying effects on cell viability. Detailed freeze-drying protocols were established, including freezing, primary drying and secondary drying phases, and the type of adjuvant. This study demonstrated the potential of freeze-dried equine PL as a viable alternative to PRP and highlighted the importance of precise freeze-drying protocols and adjuvants for standardization. Equine PL showed promise for medical treatment in horses, offering advantages such as extended shelf life, ease of handling, and reduced transportation costs, with the potential for broadened therapeutic usage.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"71-80"},"PeriodicalIF":2.8,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence and risk factors of coxiellosis in meat goat herds: an epidemiological investigation in Northeastern Thailand. 泰国东北部肉用山羊群中弓形虫病的流行情况和风险因素:一项流行病学调查。
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-07-26 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2379713
Sarinya Rerkyusuke, Sawarin Lerk-U-Suke, Peerapol Sukon, Patchara Phuektes
{"title":"Prevalence and risk factors of coxiellosis in meat goat herds: an epidemiological investigation in Northeastern Thailand.","authors":"Sarinya Rerkyusuke, Sawarin Lerk-U-Suke, Peerapol Sukon, Patchara Phuektes","doi":"10.1080/23144599.2024.2379713","DOIUrl":"10.1080/23144599.2024.2379713","url":null,"abstract":"<p><p>Q fever/coxiellosis poses a significant threat to both human and animal health, with goats serving as important reservoirs for disease transmission. This study aimed to evaluate the prevalence of coxiellosis and identify associated risk factors within meat goat herds in northeastern Thailand. A total of 39 meat goat herds were examined, with 84.61% of these herds experiencing reproductive disorders suggestive of <i>Coxiella burnetii</i> infection. Serum samples (<i>n</i> = 513) and vaginal swabs (<i>n</i> = 334) were collected from 522 goats for serological and molecular analyses, respectively. Results unveiled an overall herd prevalence of 74.35% (29/39), with a within-herd prevalence of 15.49% (95% CI: 10.86-20.12). Univariate analysis indicated that knowledge about the transmission of coxiellosis in herd owners serves as a protective factor against <i>C. burnetii</i> infection at the herd level (OR: 0.10; 95% CI: 0.01-0.92; <i>p</i> = 0.04). Multivariable analysis identified two significant risk factors associated with <i>C. burnetii</i> infection at the herd level, including herd establishment exceeding 5 years (OR: 7.14; 95% CI: 1.05-48.4; <i>p</i> = 0.04), as well as reproductive failures including abortion, infertility, and weak offspring (OR: 17.65; 95% CI: 1.76-177.45; <i>p</i> = 0.01). Individual-level risk factors included female gender (OR: 8.42; 95% CI: 1.14-62.42; <i>p</i> = 0.03), crossbreeding (OR: 2.52; 95% CI: 1.32-4.82; <i>p</i> = 0.005), and clinical signs of anaemia (OR: 1.63; 95% CI: 1.01-2.64; <i>p</i> = 0.04). These findings underscore the widespread prevalence of Q fever in meat goat herds within the study area and emphasize the necessity of implementing targeted control strategies.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"60-70"},"PeriodicalIF":2.8,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-typhoidal Salmonella in Nigeria: do outcomes of 'multisectoral' surveillance, treatment and control justify the intervention costs? 尼日利亚的非伤寒沙门氏菌:"多部门 "监测、治疗和控制的结果是否证明了干预成本的合理性?
IF 2.8
International Journal of Veterinary Science and Medicine Pub Date : 2024-07-14 eCollection Date: 2024-01-01 DOI: 10.1080/23144599.2024.2365567
Abdullahi O Sanni, Abdurrahman H Jibril, Olubunmi G Fasanmi, Oluwawemimo O Adebowale, Alexander R Jambalang, Aminu Shittu, Annelize Jonker, Latifah O Abdulkarim, Folorunso O Fasina
{"title":"Non-typhoidal <i>Salmonella</i> in Nigeria: do outcomes of 'multisectoral' surveillance, treatment and control justify the intervention costs?","authors":"Abdullahi O Sanni, Abdurrahman H Jibril, Olubunmi G Fasanmi, Oluwawemimo O Adebowale, Alexander R Jambalang, Aminu Shittu, Annelize Jonker, Latifah O Abdulkarim, Folorunso O Fasina","doi":"10.1080/23144599.2024.2365567","DOIUrl":"10.1080/23144599.2024.2365567","url":null,"abstract":"<p><p>Non-typhoidal salmonellosis (NTS) is significant and an economic burden in Nigeria. To determine whether investment in NTS control is economically justifiable, Outbreak Costing Tool (OCT) was used to estimate the robust funding of public and animal health systems for epidemio-surveillance and control of multisectoral NTS outbreaks in Nigeria. Health, production, and economic data were collected and used to populate the tool for evaluation. The multisectoral NTS burden for the year 2020 in Nigeria was US$ 930,887,379.00. Approximately 4,835 technical officers, and 3,700 non-technical staff (<i>n</i> = 8,535) were needed with an investment of >2.2 million work hours. The investment cost for NTS control was US$ 53,854,660.87. The non-labour-related cost was 89.21% of the total intervention costs. The overall intervention's investment was 374.15% of the estimated national and subnational systems' annual budget for diarrhoeal diseases, and the outbreak response period attracted the highest costs (53%) of the total intervention. In conclusion, intervention against NTS was beneficial (benefit - cost ratio: 17.29), hence justifying the need for multisectoral surveillance-response against NTS in Nigeria. Complex sectoral silos must give way to coordinated collaborations to optimize benefits; and over-centralization of health interventions' associated delays must be removed through decentralized sub-national-focused framework that empowers rapid investigation, response, control, data collection, and analyses. It should assist anticipatory planning, and outbreak investigation and reduce critical response time. Anticipatory planning tools, when applied pre-emptively, can benefit budgeting, identify gaps, and assist in the delivery of cost-saving and effective measures against infectious disease.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"48-59"},"PeriodicalIF":2.8,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141621167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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