International Journal of Veterinary Science and Medicine最新文献

{"title":"Distribution, identification and ecology of <i>Phortica</i> genus (Diptera: Drosophilidae) in Spain.","authors":"Mikel Alexander González, Marcos López-de-Felipe, Sergio Magallanes, Pedro María Alarcón-Elbal, Carlos Barceló, Yasmina Martínez-Barciela, Alejandro Polina, Ana MarîAMaría García-López, Laura Blanco-Sierra, Miguel A Peláez Guerra, Sarah Delacour, Jordi Figuerola, Ignacio Ruiz-Arrondo, Daniel Bravo-Barriga","doi":"10.1080/23144599.2025.2453960","DOIUrl":"10.1080/23144599.2025.2453960","url":null,"abstract":"<p><p>The genus <i>Phortica</i> (Diptera: Drosophilidae) includes five species of small flies in Europe. <i>Phortica variegata</i>, the zoophilic fruit fly, is the main vector of <i>Thelazia callipaeda</i>, a zoonotic parasite that is rapidly spreading througout Europe. Despite extensive studies on thelaziosis in animals and humans, there is limited knowledge about the geographical distribution and hovering activity of these vector flies. In 2023, 1,462 <i>Phortica</i> flies were sampled across 12 Spanish provinces, providing new records of <i>Phortica variegata</i> and <i>Phortica oldenbergi</i>. Surprisingly, <i>P. oldenbergi</i>, previously considered a rare Afrotropical species, was prevalent in most regions sampled in Spain. However, <i>Phortica semivirgo</i> was not collected. The abundance of <i>Phortica</i> spp. correlated positively with altitude and certain tree species. Rural oak-wooded areas in central and northern Spain showed the highest densities of <i>P. variegata</i>. Both drosophilid species were analysed morphologically and molecularly, providing new morphological descriptors and sequence barcodes for species identification. Phylogenetic analysis based on COI sequences, showed <i>P. oldenbergi</i> grouped with Asian origin <i>Phortica</i> species, while <i>P. variegata</i> in America was closer to Spanish sequences than those from other European countries. The hovering activity of <i>P. variegata</i> causes significant discomfort to humans during outdoor activities. This paper also reviews the historic records of <i>P. variegata, P. semivirgo</i> and <i>P. oldenbergi</i> in Spain over the last 90 years. This study enhances the understanding of the distribution, identification, ecology, and behaviour of these zoophilic flies in Europe.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-11"},"PeriodicalIF":2.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143469371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular analysis of cryptosporidiosis on cattle farms in Gran Canaria, Canary Islands (Spain).","authors":"María Cristina Del Río, Sergio Martín, Joaquín Quílez, Claudia Vergara-Castiblanco, José Manuel Molina, Otilia Ferrer, Magnolia María Conde, José Adrián Molina, Antonio Ruiz","doi":"10.1080/23144599.2025.2460923","DOIUrl":"10.1080/23144599.2025.2460923","url":null,"abstract":"<p><p><i>Cryptosporidium</i> spp. infections in calves cause serious economic losses in livestock and pose an important zoonotic risk. The aim of this study was to investigate the prevalence of <i>Cryptosporidium</i> spp. in cattle on the island of Gran Canaria. Faecal samples were collected from calves and adult cattle from a total of 15 farms, and a questionnaire survey was conducted to farmers. The presence of <i>Cryptosporidium</i> spp. oocysts in faeces was determined by microscopy, showing infection rates of 45.9% in calves and 4.1% in adults, with positive correlation with the faecal scores of infected animals (<i>p</i> < 0.0001). Samples were amplified by PCR targeting SSU rRNA, with positivity rates for calves and adults being 51.7% and 31.7%, respectively. The PCR-positive samples were further genotyped and sequenced for the 60 kDa glycoprotein gene (GP60) and the microsatellite TP14. Four <i>Cryptosporidium</i> species were identified (<i>C. parvum, C. ryanae</i>, <i>C. bovis,</i> and <i>C. andersoni</i>), of which <i>C.</i> <i>parvum</i> was the most frequent in calves (45.8%) and adults (29.2%). GP60 sequencing revealed that all <i>C. parvum</i> samples belonged to the IId family, the most frequent subtypes being IIdA22G1 and IIdA23G1. Overall, the results of this study demonstrate a high occurrence of <i>Cryptosporidium</i> spp. in both calves and adult cattle, including the zoonotic IId family of <i>C. parvum</i>. These findings have significant implications for cattle farming and public health. The lack of awareness among farmers regarding cryptosporidiosis highlights the need for caution to prevent epidemiological outbreaks that could impact both human and livestock health.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of <i>Cryptosporidium parvum</i>, <i>Giardia duodenalis</i> and <i>Eimeria</i> spp. in diarrhoeic suckling calves from north-western Spain and analysis of their interactions.","authors":"Cynthia López-Novo, Seila Couso-Pérez, Alberto Prieto, Jose Manuel Díaz-Cao, David García-Dios, Gonzalo López-Lorenzo, Susana Remesar, Elvira Ares-Mazás, Ceferino López, Patrocinio Morrondo, Hipólito Gómez-Couso, Pablo Díaz","doi":"10.1080/23144599.2024.2447172","DOIUrl":"10.1080/23144599.2024.2447172","url":null,"abstract":"<p><p>Although <i>Cryptosporidium parvum</i>, <i>Giardia duodenalis</i> and some <i>Eimeria</i> species are frequently involved in neonatal calf diarrhoea (NCD), detailed studies on their interactions are scarce. Therefore, a cross-sectional study including faecal samples from 404 diarrhoeic calves aged 0-30 days was performed. <i>Cryptosporidium</i> oocysts and <i>G. duodenalis</i> cysts were detected by immunofluorescence antibody test and positive samples were molecularly characterized. <i>Eimeria</i> oocysts were microscopically detected using the modified McMaster technique and morphometric species identification was performed. <i>Cryptosporidium</i> infections (53.7%) predominated over those by <i>G. duodenalis</i> (12.4%) and <i>Eimeria</i> spp. (6.9%). Three <i>Cryptosporidium</i> species were identified: <i>C. parvum</i> (98.0%), <i>C. bovis</i> (1.0%) and <i>C. ryanae</i> (1.0%). Eleven <i>C. parvum</i> zoonotic subtypes were detected, with IIaA15G2R1 as the most common; only <i>G</i>. <i>duodenalis</i> assemblage E was identified. Moreover, 10 <i>Eimeria</i> species were found, being <i>Eimeria ellipsoidalis</i> (96.4%), <i>Eimeria bovis</i> (85.7%), and <i>Eimeria zuernii</i> (71.4%) predominant. A significant positive association between <i>G. duodenalis</i> and <i>Eimeria</i> spp. was detected as well as a negative association between <i>C. parvum</i> and <i>Eimeria</i> spp. Our results showed that <i>C. parvum</i> wasprevalent in diarrhoeic calves under 21 days of life; <i>Eimeria</i> spp. and <i>G. duodenalis</i> were especially common in animals in their fourth week of life. Concurrent infections increased with age. Moreover, the study also revealed potential public health risks since a noticeable percentage of animals were infected with <i>C. parvum</i> zoonotic subtypes. Further studies are needed for determining the role of these parasites in co-infections with other enteropathogens and their implications in the pathogenicity of NCD.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-14"},"PeriodicalIF":2.8,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monocephalus dipygus in a 4-month-old mixed-breed puppy: clinical, radiographic, ultrasonographic, karyotyping and surgical intervention.","authors":"Lorena Elizabeth Chalco-Torres, Ana Elizabeth Guerrero López, Mauro Nirchio Tursellino","doi":"10.1080/23144599.2024.2418773","DOIUrl":"https://doi.org/10.1080/23144599.2024.2418773","url":null,"abstract":"<p><p>Congenital anomalies are rare in veterinary medicine, and their aetiology, development, clinical presentation, and management remain poorly understood. This study documents a rare case of multiple congenital anomalies occurring simultaneously in a single individual. We present a comprehensive analysis of a 4-month-old mixed-breed puppy diagnosed with caudal duplication (monocephalus dipygus), integrating clinical, radiographic, ultrasonographic, cytogenetic findings, and surgical intervention. The puppy exhibited several external abnormalities, including a supernumerary limb, a single tail, two penises, and two distinct scrotal sacs. Radiographic examination revealed additional metatarsal and phalangeal bones in the supernumerary limb. Ultrasonography and exploratory laparotomy further identified two bladders, a single prostate, and bifurcated ureters, each connecting to a kidney. Cytogenetic analysis was conducted to investigate potential chromosomal abnormalities underlying these congenital anomalies, as we hypothesized that these malformations might be linked to chromosomal aberrations. The successful surgical removal of the supernumerary limb significantly improved the dog's mobility. Notably, since each bladder was independently connected to a kidney, surgical intervention in the genitourinary system was avoided to prevent potential impairment of urinary function. This case highlights the complexity of congenital anomalies in canines and emphasizes the importance of a multidisciplinary approach to their diagnosis and management.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"148-156"},"PeriodicalIF":2.8,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual effects of ipecac alkaloids with potent antiviral activity against foot-and-mouth disease virus as replicase inhibitors and direct virucides.","authors":"Achiraya Pantanam, Natjira Mana, Ploypailin Semkum, Varanya Lueangaramkul, Nantawan Phecharat, Porntippa Lekcharoensuk, Sirin Theerawatanasirikul","doi":"10.1080/23144599.2024.2408189","DOIUrl":"10.1080/23144599.2024.2408189","url":null,"abstract":"<p><p>Foot-and-Mouth Disease (FMD) is a contagious, blistering disease caused by the Foot-and-Mouth Disease virus (FMDV), which affects livestock globally. Currently, no commercial antiviral agent is available for effective disease control. This study investigated the antiviral potential of natural-derived alkaloids against FMDV in BHK-21 cells. Twelve alkaloids were assessed for their antiviral activities at various stages of FMDV infection, including pre-viral entry, post-viral entry, and prophylactic assays, as well as attachment and penetration assays by evaluating cytopathic effect reduction and directed-virucidal effects. The results showed that ipecac alkaloids, cephaeline (CPL) and emetine (EMT), exhibited dual effects with robust antiviral efficacy by reducing cytopathic effect and inhibiting FMDV replication in a dose-dependent manner. Evaluation through immunoperoxidase monolayer assay and RT-PCR indicated effectiveness at post-viral entry stage, with sub-micromolar EC₅₀ values for CPL and EMT at 0.05 and 0.24 µM, respectively, and high selective indices. Prophylactic effects prevented infection with EC₅₀ values of 0.23 and 0.64 µM, respectively. Directed-virucidal effects demonstrated significant reduction of extracellular FMDV, with CPL exhibiting a dose-dependent effect. Furthermore, the replicase (3Dpol) inhibition activity was identified using the FMDV minigenome assay, which revealed strong inhibition with IC₅₀ values of 0.15 µM for CPL and 4.20 µM for EMT, consistent with the decreased negative-stranded RNA production. Molecular docking confirmed the interaction of CPL and EMT with residues in the active site of FMDV 3Dpol. In conclusion, CPL and EMT exhibited promising efficacy through their dual effects and provide an alternative approach for controlling FMD in livestock.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"134-147"},"PeriodicalIF":2.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of <i>Fasciola gigantica</i> in freshwater snail <i>Radix</i> (<i>Lymnaea</i>) spp. In the highly parasite-prevalent area of Nakhon Ratchasima Province, Thailand.","authors":"Pongsakorn Martviset, Pathanin Chantree, Amornrat Geadkaew-Krenc, Pantip Piyatadsananon, Ruttiroj Jirojwong, Chompunoot Wangboon, Mantana Jamklang, Sirilak Chumkiew, Rawipreeya Poomkhokrak, Nanthawat Kosa, Salisa Chaimon, Bumpenporn Sanannam, Rudi Grams, Wansika Phadungsil","doi":"10.1080/23144599.2024.2396700","DOIUrl":"https://doi.org/10.1080/23144599.2024.2396700","url":null,"abstract":"<p><p>This study investigates the distribution of the <i>Lymnaea</i> (<i>Radix</i>) spp. in Pak Chong district, Nakhon Ratchasima province, northeast Thailand, where a vast cattle farming area is located and has a high prevalence of <i>Fasciola</i> spp. in the cattle. By random selection, 1,414 snails were collected from the natural and man-made ponds. The snails were recorded for morphology and processed for DNA isolation. The snail species were investigated by conventional PCR using a 16S rDNA-specific primer. The result demonstrated that all collected snails were <i>R</i>. (<i>L</i>.) <i>rubiginosa</i>. Moreover, the infection of <i>Fasciola</i> gigantica in the snails was investigated by PCR using a cytochrome c oxidase I (COX1)-specific primer. The results illustrated that the overall prevalence was 22.5% (318/1414), with the highest prevalence in the Nong Sa Rai subdistrict at 73.6% (81/110), which is the highest prevalence of <i>Fasciola gigantica</i> in the snail host that has ever been reported. The lowest prevalence existed in the Pong Ta Long subdistrict at 3.7% (4/109). Our results corresponded to the previous report on the <i>Fasciola</i> spp. infection in the cattle from this area, and the geographical analysis revealed that the most suspected factor would be the earth dam located in these subdistricts, where many animals live freely during the day. Our findings could be helpful for further parasite control and could trigger the study of the biology and associated factors in the future.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"125-133"},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructural architecture of the bony scutes, spine, and rays of the bony fins in the common pleco <i>(Hypostomus plecostomus)</i>.","authors":"Hanan H Abd-Elhafeez, Diaa Massoud, Mohammed S Mahmoud, Nada Abdellah, Abdallah S Salah, Nor-Elhoda Mohamed, Mennatallah Ali Abdelrhman Sayed, Mohamed Shaalan, Catrin S Rutland, Alaa Sayed Abu-ELhamed, Soha A Soliman, Fatma El-Zahraa A Mustafa","doi":"10.1080/23144599.2024.2374201","DOIUrl":"10.1080/23144599.2024.2374201","url":null,"abstract":"<p><p>Studying scute and fin morphology are advantageous approaches for phylogenetic identification and provide information on biological linkages and evolutionary history that are essential for deciphering the fossil record. Despite this, no prior research has precisely characterized the histological structures of scutes in the common pleco. Therefore, this research investigated the microstructure and organization of bone tissue within the dermal skeleton, including the scutes and fins, in the common pleco, using light microscopy, stereomicroscopy, and scanning electron microscopy. The dermal scutes were organized in a pentagonal shape with denticular coverage and were obliquely aligned with the caudal portion pointing dorsally. The dermal scutes consisted of three distinct portions: the central, preterminal, and terminal portions. Each portion comprised three layers: a superficial bony plate, a basal bony plate, and a mid-plate. Both the superficial and basal bony plates were composed of lamellar bone and lamellar zonal bone, whilst the mid-plate consisted of secondary osteons and woven bone. In the terminal portion, the superficial and basal bony plates became thinner. The pectoral fin consists of spines and rays composed of lepidotrichium (two symmetrical hemi-rays). The spine contained centrifugal and centripetal lamellar and trabecular bones. A centripetal fibrous bone was implanted between the lamellar bones. Besides being oriented in a V shape, the hemi-rays were also composed of thin centrifugal and centripetal lamellar bones and trabecular bones. A fibrous bone was identified between the centrifugal and centripetal bones. The trabecular bone and lamellar bone were made up of bone spicules.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"101-124"},"PeriodicalIF":2.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and molecular detection of <i>Brucella</i> infection in livestock in the United Arab Emirates.","authors":"Gobena Ameni, Aboma Zewude, Berecha Bayissa, Ibrahim Abdalla Alfaki, Abdallah A Albizreh, Naeema Alhosani, Khaja Mohteshamuddin, Berhanu Adenew Degefa, Mohamed Elfatih Hamad, Meera Saeed Alkalbani, Mohamed Moustafa Abdelhalim, Assem Sobhi Abdelazim, Rafeek Aroul Koliyan, Kaltham Kayaf, Mervat Mari Al Nuaimat, Robert Barigye, Arve Lee Willingham, Markos Tibbo, Bedaso Mammo Edo, Teshale Sori, Yassir Mohammed Eltahir","doi":"10.1080/23144599.2024.2384015","DOIUrl":"10.1080/23144599.2024.2384015","url":null,"abstract":"<p><p>Small ruminants and camels are important livestock species in the United Arab Emirates (UAE), although <i>Brucella</i> infection can limit their productivity. This study aimed to investigate the seroprevalence of <i>Brucella</i> infection and its associated risk factors in small ruminants and camels in the Emirate of Abu Dhabi. Additionally, seropositive animals were tested for the DNA of <i>Brucella</i>. Multispecies competitive enzyme-linked immunosorbent assay (c-ELISA) and multispecies indirect (i-ELISA) were used to test 3,086 animals from 2022 to 2023. <i>Brucella</i> cell surface 31 kDa protein (bcsp31) gene-based real-time polymerase chain reaction (q-PCR) was used to detect <i>Brucella</i> DNA. Multivariate logistic regression was used to assess the association between seroprevalence and potential risk factors. The overall seroprevalences of <i>Brucella</i> infection were 1.7% (95% confidence interval [CI], 1.2%-2.2%) and 5.8% (95% CI, 5.0%-6.7%) based on serial and parallel testing, respectively. The DNA of <i>Brucella</i> was detected in 13 of the 51 seropositive animals. The overall seroprevalence of <i>Brucella</i> infection was associated with the region, type of animal holding, species, and age of the animals. In conclusion, this study documented <i>Brucella</i> infection in small ruminants and camels in the Emirate of Abu Dhabi, warranting necessary intervention strategies to eliminate <i>Brucella</i> infections in livestock populations.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"91-100"},"PeriodicalIF":2.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11346334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of induced pluripotent stem cells from the Asian bats.","authors":"Younsu Lee, Okjae Koo, Islam M Saadeldin","doi":"10.1080/23144599.2024.2384835","DOIUrl":"10.1080/23144599.2024.2384835","url":null,"abstract":"<p><p>Preservation of native Korean bats is crucial for maintaining ecological balance, as they play a vital role in insect control, pollination, and seed dispersal within their ecosystems. The present study details the establishment of bat induced pluripotent stem cells (BatiPSCs) from two Asian and Korean bats (<i>Hypsugo alaschanicus</i> and <i>Pipistrellus abramus</i>) using the Sendai Reprogramming Kit. Colonies of BatiPSCs, exhibiting distinctive features, were manually selected and expanded following successful transfection. Characterization of BatiPSCs revealed the expression of pluripotency markers, such as Octamer-binding transcription factor 4 (Oct4), SRY (sex-determining region Y)-box 2 and Nanog, with notably increased Oct4 levels and reduced Myc proto-oncogene expression compared with those noted in other induced pluripotent stem cell sources. BatiPSCs displayed positive staining for alkaline phosphatase and demonstrated the ability to form embryoid bodies, while also inducing teratomas in non-immune nude mice. Additionally, green fluorescent protein (GFP)-expressing BatiPSCs were generated and used for chimeric mouse production, with slight GFP signals detected in the neck region of the resulting mouse foetuses. These findings demonstrate the successful generation and characterization of BatiPSCs, emphasizing their potential applications in chimeric animal models, and the protection of endangered bat species.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"81-90"},"PeriodicalIF":2.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate.","authors":"Chiara Bernardini, Noemi Romagnoli, Isabelle Casalini, Maria Elena Turba, Alessandro Spadari, Monica Forni, Fabio Gentilini","doi":"10.1080/23144599.2024.2380586","DOIUrl":"10.1080/23144599.2024.2380586","url":null,"abstract":"<p><p>Platelet lysate, derived from platelets, are valuable biological products rich in bioactive molecules. Their use promotes tissue healing and modulates inflammation. However, maintaining the stability and bioactivity of platelet lysate is challenging since they degrade rapidly at room temperature. This study focused on the possibility to confer enhanced stability to freeze-dried equine platelet lysate as an alternative to platelet-rich plasma (PRP). Platelet lysate (PL) was derived from PRP and freeze-dried either as such or using various adjuvants. Primary cell cultures of porcine Vascular Wall-Mesenchymal Stem Cells were treated with different PL formulations, and cell viability was assessed using an MTT assay. Overall, the addition of PL significantly improved cell viability as compared to controls without growth factor supplementation or with foetal bovine serum. Notably, the freeze-drying process maintained the effectiveness of the PL for at least a week. Furthermore, the study revealed that varying the horse as the source of PL could yield varying effects on cell viability. Detailed freeze-drying protocols were established, including freezing, primary drying and secondary drying phases, and the type of adjuvant. This study demonstrated the potential of freeze-dried equine PL as a viable alternative to PRP and highlighted the importance of precise freeze-drying protocols and adjuvants for standardization. Equine PL showed promise for medical treatment in horses, offering advantages such as extended shelf life, ease of handling, and reduced transportation costs, with the potential for broadened therapeutic usage.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"71-80"},"PeriodicalIF":2.8,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}