International Journal of Veterinary Science and Medicine最新文献

{"title":"Neonatal Blood Count Data of Piglets in Modern and Traditional Breeds following Spontaneous or Hormonally Induced Parturition.","authors":"Michael Oster, Heinke Lickfett, Andreas Vernunft, Henry Reyer, Eduard Muráni, Hartwig Bostedt, Klaus Wimmers","doi":"10.4103/ijvsm.ijvsm_5_26","DOIUrl":"10.4103/ijvsm.ijvsm_5_26","url":null,"abstract":"<p><strong>Background: </strong>Traditional pig breeds experience a renaissance in public perception to favor biodiversity and resilience to diseases. Moreover, the routine use of parturition induction with exogenous hormones is questioned, as it may compromise the welfare of both dams and piglets.</p><p><strong>Aims and objectives: </strong>In this study, neonatal complete blood counts were analysed regarding the effects of genotype, i.e., traditional German Saddleback versus modern German Landrace, and the implementation or omission of parturition induction by hormonal intervention.</p><p><strong>Materials and methods: </strong>Blood samples of <i>n</i> = 296 newborn piglets from 28 farrowings were taken within the first 6 h of life to study hematological traits.</p><p><strong>Results: </strong>The population showed 22% of neonates being anaemic with hemoglobin <11.0 g/dl (<i>P</i> < 0.01). The Saddleback piglets exhibited higher red blood cell count and lower mean corpuscular volume and mean corpuscular hemoglobin levels than Landrace piglets (<i>P</i> < 0.001). The Landrace piglets exhibited higher white blood cell count, neutrophil count, and neutrophil-to-lymphocyte ratio than Saddleback piglets (<i>P</i> < 0.001). The hormonal parturition induction revealed no effects for the analysed indices except for red cell distribution width-coefficient of variation with higher values in spontaneously born piglets compared with piglets that experienced an induced parturition (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>The pronounced differences between Saddleback and Landrace neonates suggest a breed-specific setting of the hematological activity at birth, which might be partially modulated by hormone application due to labor induction in pigs.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"14 ","pages":"6"},"PeriodicalIF":2.3,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13175203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147965121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Accuracy and Efficiency of Canine Synovial Fluid Analysis with Hyaluronidase Pretreatment and Automated Analyzer.","authors":"Winda Kusuma Dewi, Araya Radtanakatikanon, Ekkapol Akaraphutiporn, Teerawut Nedumpun, Chalika Wangdee","doi":"10.4103/ijvsm.ijvsm_6_26","DOIUrl":"10.4103/ijvsm.ijvsm_6_26","url":null,"abstract":"<p><strong>Background: </strong>Synovial fluid examination is vital for diagnosing joint diseases in dogs. However, conventional manual methods are labor-intensive and prone to imprecision. Pretreating synovial fluid with hyaluronidase and utilizing an automated analyzer may enhance the accuracy, precision, and efficiency of synovial fluid analysis, particularly in dogs.</p><p><strong>Aims and objectives: </strong>This study aimed to assess the accuracy, precision, and efficiency of automated analyzer compared with manual cell count (MC) for analyzing synovial fluid in dogs.</p><p><strong>Materials and methods: </strong>Fifty-two synovial fluid samples with varying cellularity were evaluated for the total nucleated cell count (TNCC) and differential cell count using three methods: hyaluronidase-treated automated cell count (AC-Hy), hyaluronidase-treated MC (MC-Hy), and untreated MC. The accuracy and precision of each method were statistically compared.</p><p><strong>Results: </strong>AC-Hy and MC-Hy provide the strongest agreement in determining TNCC (mean difference: 23.92%, 95% confidence interval: -53.23%-101.07%), with AC-Hy exhibiting the highest precision (coefficient of variations [CV]: 4.42%-4.49%). For differential counts, AC-Hy performed well in high TNCC samples (CV: 0.74%-2.25%) but was less reliable in low TNCC samples. MC-Hy, however, demonstrated consistent accuracy in determining differential count across all TNCC levels and produced superior smear quality compared with MC. In addition, AC-Hy offers shorter analytical times and is technically more efficient.</p><p><strong>Conclusion: </strong>In conclusion, pretreating canine synovial fluid with hyaluronidase enhances the accuracy and precision of synovial fluid analysis. While an automated analyzer enables rapid TNCC measurement, its reliability for differential counts decreases at low TNCC. Integrating automated count with hyaluronidase-treated manual differential counts and clinical evaluations would enhance diagnostic precision.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"14 ","pages":"3"},"PeriodicalIF":2.3,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13175205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147965139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel LC-MS/MS Method for Monitoring Perfluoroalkyl and Polyfluoroalkyl Substances in Companion Animal Blood Plasma and Serum: Environmental and Health Implications.","authors":"Anisa Bardhi, Francesca Ferretti, Elena Serrantoni, Andrea Barbarossa","doi":"10.4103/ijvsm.ijvsm_9_26","DOIUrl":"10.4103/ijvsm.ijvsm_9_26","url":null,"abstract":"<p><strong>Background: </strong>Per- and poly-fluoroalkyl substances (PFAS), known as \"forever chemicals,\" are synthetic compounds widely used for their unique physicochemical properties. Their environmental persistence and potential link to adverse health outcomes have raised significant concerns, necessitating robust monitoring methods. While numerous studies have quantified PFAS in human biological fluids, there is a critical gap in the availability of validated analytical techniques for companion animals remain scares.</p><p><strong>Aims and objectives: </strong>The aim of this study was to develop and validate a novel analytical method for the quantification of eleven PFAS in biological samples from companion animals, specifically canine serum and feline plasma, and to apply this method to real samples.</p><p><strong>Materials and methods: </strong>A streamlined sample preparation protocol was developed including protein precipitation, evaporation under nitrogen at 45°C, and reconstitution. Quantification was performed by ultra-high-performance liquid chromatography-tandem mass spectrometry in negative electrospray mode, monitoring two transitions for each compound.</p><p><strong>Results: </strong>The method was validated according to European Medicines Agency guidelines and demonstrated excellent analytical performance, with strong linearity (<i>R</i>² ≥ 0.99), high precision (CV% < 15%), and accuracy within ±15% of nominal concentrations. Recovery ranged from 80.0% to 114.9% and process efficiency from 80.7% to 114.0%, with negligible matrix effects and no observable carry-over. Application to real samples from 23 healthy dogs and 4 healthy cats revealed PFAS concentrations ranging from 0.1 to 15.8 µg/L in dogs and from 0.1 to 2.8 µg/L in cats. PFOS was the most prevalent compound in both species, with PFHxS also prominent in cats.</p><p><strong>Conclusion: </strong>The validated UHPLC-MS/MS method provides a reliable and efficient tool for the biomonitoring of PFAS in companion animals. Its application enables large-scale assessment of environmental exposure and supports the evaluation of potential health implications for both pets and their owners.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"14 ","pages":"1"},"PeriodicalIF":2.3,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13175208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147965061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging Evaluation of Structural Changes in the Cisterna Magna Region Associated with Cervical Flexion in Cockatiels.","authors":"Yuta Katsuno, Kazuya Kushida, Miori Kishimoto, Sanae Shibata, Toshie Iseri, Tomoko Iwanaga, Ryuji Fukushima, Sakura Isayama, Qingling Sun, Yukiko Sassa-O'Brien","doi":"10.4103/ijvsm.ijvsm_8_26","DOIUrl":"10.4103/ijvsm.ijvsm_8_26","url":null,"abstract":"<p><strong>Background: </strong>Cisternal puncture is a widely used technique for cerebrospinal fluid collection, with significant advancements in safety achieved through diagnostic imaging in mammals. However, its use in small avian species remains limited due to anatomical constraints and anesthetic risks. A comprehensive understanding of cisterna magna anatomy in birds is essential for optimizing cisternal puncture safety and enhancing central nervous system access.</p><p><strong>Aim and objectives: </strong>This study aimed to evaluate cisterna magna morphology in cockatiels (<i>Nymphicus hollandicus</i>) using computed tomography (CT) and magnetic resonance imaging (MRI), focusing on structural changes associated with cervical flexion.</p><p><strong>Materials and methods: </strong>Six cockatiels underwent sedation and anesthesia for imaging. Positioned in supine with cervical flexion, anatomical modifications were assessed.</p><p><strong>Results: </strong>Intranasal sedation and anesthesia facilitated high-quality imaging with no adverse outcomes. CT demonstrated that cervical flexion increased the atlas-occipital distance from 1.93 mm (extended) to 3.09 mm (flexed) and the angle from 80.21° to 150.8°. MRI of one individual revealed an increase in cisterna magna cross-sectional area from 0.88 mm² to 3.42 mm², though sufficient flexion was not achieved in others.</p><p><strong>Discussions and conclusions: </strong>These findings suggest that cervical flexion may facilitate cisterna magna expansion. This study highlights the feasibility of using CT and MRI for cisterna magna assessment in cockatiels and suggests that cervical flexion may enhance its cross-sectional area. These results contribute to advancing avian neurology and may improve cisternal puncture safety in small avian species.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"14 ","pages":"2"},"PeriodicalIF":2.3,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13175204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147965113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosemary Essential Oil as a Natural Disinfectant in Japanese Quail Eggs (<i>Coturnix Coturnix Japonica</i>): Effect on the Bacterial Load and Hatchability Characteristics.","authors":"Leghel Touazi, Assia Aberbour, Katia Djenadi, Amine Benberkane, Sofiane Aissanou, Mourad Boumenir, Mokrane Iguer-Ouada, Jean-Luc Hornick, Nassim Moula","doi":"10.4103/IJVSM.IJVSM_1_25","DOIUrl":"10.4103/IJVSM.IJVSM_1_25","url":null,"abstract":"<p><strong>Background: </strong>Maintaining hygiene in hatcheries is crucial for improving hatchability and ensuring healthy chick performance while reducing the risk of food contamination. Bacterial and fungal contamination, particularly on eggshells, is a major concern in the poultry industry. Egg disinfection before incubation has garnered global interest, especially with the rise in demand for natural alternatives to conventional disinfectants, which are often toxic and can lead to bacterial resistance.</p><p><strong>Aims and objectives: </strong>This study evaluated the potential of rosemary essential oil (REO) as a natural disinfectant for eggshells and its impact on hatching success.</p><p><strong>Materials and methods: </strong>A total of 216 Japanese quail eggs were divided into five groups: A negative control (nondisinfected), two positive controls treated with alcohol or SANIVIR (Quaternary Ammonium) (10%), and two groups disinfected with REO at the concentrations of 10 µl/ml and 100 µl/ml. Eggshells were swabbed to measure bacterial, coliform, fungal, and mold counts using serial dilutions and agar cultures. Hatching rate, chick weight, and mortality were also recorded.</p><p><strong>Results: </strong>The findings revealed that REO effectively reduced bacterial loads, with the 10 µl/ml group achieving the lowest contamination levels and significantly higher hatchability. In addition, chicks from this group had a greater average body weight. However, the 100 µl/ml group experienced higher embryonic mortality, likely due to its stronger concentration.</p><p><strong>Conclusion: </strong>In conclusion, REO at 10 µl/ml demonstrates promise as a safe and natural eggshell disinfectant. Its use not only improved hatchability but also positively influenced chick performance, making it a valuable alternative in commercial poultry operations.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 ","pages":"1"},"PeriodicalIF":2.3,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13080716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147934440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intravenous Administration of Hyaluronan in Veterinary Musculoskeletal Disorders: Therapeutic Applications and Mechanisms.","authors":"Daniela Rubanová, Kristina Nešporová, Lukáš Kubala","doi":"10.4103/IJVSM.IJVSM_2_25","DOIUrl":"10.4103/IJVSM.IJVSM_2_25","url":null,"abstract":"<p><p>Hyaluronan (HA), a natural polysaccharide, is a significant component in the extracellular matrix of connective tissues. Recently, HA has gained substantial attention in veterinary medicine due to its remarkable effects in promoting wound healing and supporting joint health. The route of administration plays a key role in the therapeutic potential of HA, as it affects the pharmacokinetics and bioavailability of HA within the body and diseased joints. Moreover, administration routes differ considerably regarding technical complexity and patient discomfort. This review summarizes recent studies exploring therapeutic applications of intravenous HA across various medical conditions, animal species, and clinical settings, emphasizing specific therapeutic effects, underlying mechanisms, clinical efficacy, and overall therapeutic potential, highlighting systemic effects beneficial for multijoint and systemic connective tissue conditions.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 ","pages":"2"},"PeriodicalIF":2.3,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13080718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147934430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cumulative Effects of Cannabis Oils on Body Temperature, Electrocardiography, Heart Rate Variability, Blood Pressure, and Respiratory Function in Telemetered Dogs.","authors":"Nattapat Rutjanavate, Van Nhut Khanh Dong, Suwanakiet Sawangkoon","doi":"10.4103/IJVSM.IJVSM_3_25","DOIUrl":"10.4103/IJVSM.IJVSM_3_25","url":null,"abstract":"<p><strong>Background: </strong>Cannabis oils are progressively being investigated as potential treatments for various health conditions in dogs. However, their safety profiles in this species remain poorly understood.</p><p><strong>Objectives: </strong>This study evaluated the effects of incremental doses of cannabis oils containing tetrahydrocannabinol (THC), cannabidiol (CBD), and their combination (THC + CBD) on cardiovascular and respiratory parameters in dogs.</p><p><strong>Materials and methods: </strong>Six male Beagles participated in a 3 × 3 crossover study. Acute cumulative doses (1, 2, 4, 8, and 16 mg/kg) were administered via the oromucosal route at 2-h intervals. Measurements included rectal temperature, electrocardiography, heart rate variability, blood pressure, and respiratory plethysmography.</p><p><strong>Results: </strong>All dogs tolerated the treatments, although adverse clinical signs were observed with THC and THC + CBD, including shivering and vomiting, with THC alone additionally causing ataxia and urinary incontinence. Both THC and THC + CBD caused significant hypothermia starting at a dose of 2 mg/kg, and they also shortened PQ intervals at doses of 4, 8, and 16 mg/kg. THC also significantly prolonged RR and QT intervals at doses of 2, 4, and 8 mg/kg, but not THC + CBD. CBD tended to increase standard deviation of normal RR intervals, root mean square of successive differences, and total power at doses of 8 and 16 mg/kg, while THC and THC + CBD had no significant effect on these parameters. These oils did not produce significant effects on blood pressure or respiratory function within these dose ranges.</p><p><strong>Conclusions: </strong>CBD oil was well-tolerated, whereas THC and THC + CBD oils induced significant side effects starting at 2 mg/kg. This study establishes a basis for determining appropriate cannabis oil dosages for future clinical studies in dogs.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 ","pages":"3"},"PeriodicalIF":2.3,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13080719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147934446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of growth rate, haematologic, and biochemical parameters of Oncopigs.","authors":"Lobna Elkhadragy, Caitlyn C Castillo, Rena Li, Courtni R Bolt, Laurie Rund, Faith Thomas, Rachel Lane, Ron C Gaba, Lawrence B Schook, Kyle M Schachtschneider","doi":"10.1080/23144599.2025.2502711","DOIUrl":"10.1080/23144599.2025.2502711","url":null,"abstract":"<p><p>Pigs are widely used as large animal models in biomedical research due to their physiological and anatomical similarity to humans. The Oncopig, a genetically engineered pig model harbouring Cre recombinase-inducible <i>KRAS</i> ^<i>G12D</i> and <i>TP53</i> ^<i>R167H</i> transgenes, serves as a valuable model for cancer research. This study describes the generation of Oncopig breeding herds and provides a characterization of their growth rate, body size, and physiological parameters, including haematologic, biochemical, and coagulation profiles. Body weight and size were measured in male and female Oncopigs, and blood samples were collected at multiple time points from birth to one year of age. A total of 13 haematologic, 18 biochemical, and 3 coagulation parameters were analysed. While male and female Oncopigs had a comparable growth rate within the first 6 months of age, male Oncopigs exhibited a significantly higher growth rate between 6 and 12 months of age and a higher body weight at 12 months. The mean body weight at 6 months of age was 52.3 kg for male Oncopigs and 48.0 kg for female Oncopigs, while at 11 months of age it was 96.4 kg for male Oncopigs and 71.7 kg for female Oncopigs. Haematologic, biochemical, and coagulation parameters were analysed for Oncopigs under 6 months of age, over 6 months, and collectively for Oncopigs within a year of age. By providing comprehensive data on growth, haematologic, and serum biochemical parameters, this study provides a critical resource for researchers utilizing Oncopigs as large animal models for cancer research and other translational studies.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-9"},"PeriodicalIF":3.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-operative supplementation with vitamin D after mucogingival surgery significantly enhances autophagy and improves life quality following feline chronic gingivostomatitis.","authors":"Yung-Kai Huang, Chung-Wei Chen, Ting-Yi Renn, Ming-Yi Shen, Igor V Klimenkov, Nikolay P Sudakov, Sandeep Kumar Singh, Hung-Ming Chang","doi":"10.1080/23144599.2025.2487751","DOIUrl":"https://doi.org/10.1080/23144599.2025.2487751","url":null,"abstract":"<p><p>Oral mucositis is a complex inflammatory and ulcerative condition frequently associated with a heightened risk of infections, malnutrition and diminished quality of life in both humans and animals. Despite the availability of various preventive and therapeutic interventions, their overall efficacy remains unclear. Considering that vitamin D exhibits pronounced anti-inflammatory properties by modulating autophagic pathways via activation of the vitamin D receptor (VDR), the present study aims to determine whether supplementation with vitamin D after the mucogingival replacement surgery (MGRS) would effectively enhance autophagy, and therefore, protect the integrity of mucosal lining in cases of severe oral mucositis. Adult domestic cats suffered from feline chronic gingivostomatitis and undergoing MGRS were used in this study. After MGRS, experimental cats were orally administered either fat-soluble or water-soluble vitamin D at a dose of 200 ng/kg twice daily for 6 weeks. Quantitative analysis revealed that in cats with oral mucositis and received MGRS, post-operative supplementation of both types of vitamin D greatly improved the quality of life and increased the anti-inflammatory reactivity. Moreover, both types of vitamin D considerably enhanced the expression of VDR and light chain 3B (LC3B, a biochemical marker for autophagy) within the affected tissues, with the most notable change observed in cats that received fat-soluble vitamin D. Based on these findings, incorporating vitamin D into the post-operative care regimens may enhance the therapeutic efficacy of surgical interventions targeting severe mucosal injury. This strategy may also hold a novel promise for improving the overall management of oral mucositis and associated complications.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-9"},"PeriodicalIF":2.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12024499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling HER2 immunoexpression in canine hepatoid gland neoplasms: clinicopathological and morphological associations.","authors":"Hassadin Boonsriroj, Sahatchai Tangtrongsup, Jirapat Arunorat, Thanongsak Mamom, Pinkarn Chantawong","doi":"10.1080/23144599.2025.2495522","DOIUrl":"https://doi.org/10.1080/23144599.2025.2495522","url":null,"abstract":"<p><p>Canine hepatoid gland neoplasms (HGNs) are significant clinical concerns due to their high prevalence and diverse biological behaviour. Human epidermal growth factor receptor 2 (HER2), a tyrosine kinase receptor implicated in various aspects of tumorigenesis, has been extensively studied in human and animal neoplasms but remains unexplored in HGNs. This study aimed to assess HER2 immunoexpression in canine HGNs and its association with clinicopathological and morphological features. A total of 61 formalin-fixed paraffin-embedded samples, including normal hepatoid glands (<i>n</i> = 10), hepatoid gland adenomas (HGAs, <i>n</i> = 20), hepatoid gland epitheliomas (HGEs, <i>n</i> = 16), and hepatoid gland carcinomas (HGCs, <i>n</i> = 15), were analysed using immunohistochemistry. HER2 expression was scored based on percentage positivity and staining intensity. HER2-positive expression was detected in 50% of HGEs (score 2  +  ) and 73.3% of HGCs, with 36.4% of cases scoring 3   +   . In contrast, all HGAs and normal hepatoid tissues were HER2-immunonegative. Statistical analysis revealed significant differences in HER2 expression among normal and neoplastic hepatoid glands (<i>p</i> < 0.001). Only in HGCs, HER2 expression was significantly associated with tissue invasion (<i>p</i> = 0.007), mitotic count (<i>p</i> = 0.033), and nuclear pleomorphism (<i>p</i> = 0.007). These findings suggest that HER2 may play a role in the progression of malignant HGNs, particularly HGCs. This preliminary study highlights the potential of HER2 as a diagnostic marker and emphasizes the need for further investigation into its prognostic value and role in HER2-targeted therapy for canine HGCs.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"13 1","pages":"1-12"},"PeriodicalIF":2.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12024500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}