International Journal of Veterinary Science and Medicine最新文献

{"title":"Seroprevalence and molecular detection of <i>Brucella</i> infection in livestock in the United Arab Emirates.","authors":"Gobena Ameni, Aboma Zewude, Berecha Bayissa, Ibrahim Abdalla Alfaki, Abdallah A Albizreh, Naeema Alhosani, Khaja Mohteshamuddin, Berhanu Adenew Degefa, Mohamed Elfatih Hamad, Meera Saeed Alkalbani, Mohamed Moustafa Abdelhalim, Assem Sobhi Abdelazim, Rafeek Aroul Koliyan, Kaltham Kayaf, Mervat Mari Al Nuaimat, Robert Barigye, Arve Lee Willingham, Markos Tibbo, Bedaso Mammo Edo, Teshale Sori, Yassir Mohammed Eltahir","doi":"10.1080/23144599.2024.2384015","DOIUrl":"10.1080/23144599.2024.2384015","url":null,"abstract":"<p><p>Small ruminants and camels are important livestock species in the United Arab Emirates (UAE), although <i>Brucella</i> infection can limit their productivity. This study aimed to investigate the seroprevalence of <i>Brucella</i> infection and its associated risk factors in small ruminants and camels in the Emirate of Abu Dhabi. Additionally, seropositive animals were tested for the DNA of <i>Brucella</i>. Multispecies competitive enzyme-linked immunosorbent assay (c-ELISA) and multispecies indirect (i-ELISA) were used to test 3,086 animals from 2022 to 2023. <i>Brucella</i> cell surface 31 kDa protein (bcsp31) gene-based real-time polymerase chain reaction (q-PCR) was used to detect <i>Brucella</i> DNA. Multivariate logistic regression was used to assess the association between seroprevalence and potential risk factors. The overall seroprevalences of <i>Brucella</i> infection were 1.7% (95% confidence interval [CI], 1.2%-2.2%) and 5.8% (95% CI, 5.0%-6.7%) based on serial and parallel testing, respectively. The DNA of <i>Brucella</i> was detected in 13 of the 51 seropositive animals. The overall seroprevalence of <i>Brucella</i> infection was associated with the region, type of animal holding, species, and age of the animals. In conclusion, this study documented <i>Brucella</i> infection in small ruminants and camels in the Emirate of Abu Dhabi, warranting necessary intervention strategies to eliminate <i>Brucella</i> infections in livestock populations.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"91-100"},"PeriodicalIF":2.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11346334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of induced pluripotent stem cells from the Asian bats.","authors":"Younsu Lee, Okjae Koo, Islam M Saadeldin","doi":"10.1080/23144599.2024.2384835","DOIUrl":"10.1080/23144599.2024.2384835","url":null,"abstract":"<p><p>Preservation of native Korean bats is crucial for maintaining ecological balance, as they play a vital role in insect control, pollination, and seed dispersal within their ecosystems. The present study details the establishment of bat induced pluripotent stem cells (BatiPSCs) from two Asian and Korean bats (<i>Hypsugo alaschanicus</i> and <i>Pipistrellus abramus</i>) using the Sendai Reprogramming Kit. Colonies of BatiPSCs, exhibiting distinctive features, were manually selected and expanded following successful transfection. Characterization of BatiPSCs revealed the expression of pluripotency markers, such as Octamer-binding transcription factor 4 (Oct4), SRY (sex-determining region Y)-box 2 and Nanog, with notably increased Oct4 levels and reduced Myc proto-oncogene expression compared with those noted in other induced pluripotent stem cell sources. BatiPSCs displayed positive staining for alkaline phosphatase and demonstrated the ability to form embryoid bodies, while also inducing teratomas in non-immune nude mice. Additionally, green fluorescent protein (GFP)-expressing BatiPSCs were generated and used for chimeric mouse production, with slight GFP signals detected in the neck region of the resulting mouse foetuses. These findings demonstrate the successful generation and characterization of BatiPSCs, emphasizing their potential applications in chimeric animal models, and the protection of endangered bat species.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"81-90"},"PeriodicalIF":2.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freeze-drying protocols and methods of maintaining the in-vitro biological activity of horse platelet lysate.","authors":"Chiara Bernardini, Noemi Romagnoli, Isabelle Casalini, Maria Elena Turba, Alessandro Spadari, Monica Forni, Fabio Gentilini","doi":"10.1080/23144599.2024.2380586","DOIUrl":"10.1080/23144599.2024.2380586","url":null,"abstract":"<p><p>Platelet lysate, derived from platelets, are valuable biological products rich in bioactive molecules. Their use promotes tissue healing and modulates inflammation. However, maintaining the stability and bioactivity of platelet lysate is challenging since they degrade rapidly at room temperature. This study focused on the possibility to confer enhanced stability to freeze-dried equine platelet lysate as an alternative to platelet-rich plasma (PRP). Platelet lysate (PL) was derived from PRP and freeze-dried either as such or using various adjuvants. Primary cell cultures of porcine Vascular Wall-Mesenchymal Stem Cells were treated with different PL formulations, and cell viability was assessed using an MTT assay. Overall, the addition of PL significantly improved cell viability as compared to controls without growth factor supplementation or with foetal bovine serum. Notably, the freeze-drying process maintained the effectiveness of the PL for at least a week. Furthermore, the study revealed that varying the horse as the source of PL could yield varying effects on cell viability. Detailed freeze-drying protocols were established, including freezing, primary drying and secondary drying phases, and the type of adjuvant. This study demonstrated the potential of freeze-dried equine PL as a viable alternative to PRP and highlighted the importance of precise freeze-drying protocols and adjuvants for standardization. Equine PL showed promise for medical treatment in horses, offering advantages such as extended shelf life, ease of handling, and reduced transportation costs, with the potential for broadened therapeutic usage.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"71-80"},"PeriodicalIF":2.8,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and risk factors of coxiellosis in meat goat herds: an epidemiological investigation in Northeastern Thailand.","authors":"Sarinya Rerkyusuke, Sawarin Lerk-U-Suke, Peerapol Sukon, Patchara Phuektes","doi":"10.1080/23144599.2024.2379713","DOIUrl":"10.1080/23144599.2024.2379713","url":null,"abstract":"<p><p>Q fever/coxiellosis poses a significant threat to both human and animal health, with goats serving as important reservoirs for disease transmission. This study aimed to evaluate the prevalence of coxiellosis and identify associated risk factors within meat goat herds in northeastern Thailand. A total of 39 meat goat herds were examined, with 84.61% of these herds experiencing reproductive disorders suggestive of <i>Coxiella burnetii</i> infection. Serum samples (<i>n</i> = 513) and vaginal swabs (<i>n</i> = 334) were collected from 522 goats for serological and molecular analyses, respectively. Results unveiled an overall herd prevalence of 74.35% (29/39), with a within-herd prevalence of 15.49% (95% CI: 10.86-20.12). Univariate analysis indicated that knowledge about the transmission of coxiellosis in herd owners serves as a protective factor against <i>C. burnetii</i> infection at the herd level (OR: 0.10; 95% CI: 0.01-0.92; <i>p</i> = 0.04). Multivariable analysis identified two significant risk factors associated with <i>C. burnetii</i> infection at the herd level, including herd establishment exceeding 5 years (OR: 7.14; 95% CI: 1.05-48.4; <i>p</i> = 0.04), as well as reproductive failures including abortion, infertility, and weak offspring (OR: 17.65; 95% CI: 1.76-177.45; <i>p</i> = 0.01). Individual-level risk factors included female gender (OR: 8.42; 95% CI: 1.14-62.42; <i>p</i> = 0.03), crossbreeding (OR: 2.52; 95% CI: 1.32-4.82; <i>p</i> = 0.005), and clinical signs of anaemia (OR: 1.63; 95% CI: 1.01-2.64; <i>p</i> = 0.04). These findings underscore the widespread prevalence of Q fever in meat goat herds within the study area and emphasize the necessity of implementing targeted control strategies.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"60-70"},"PeriodicalIF":2.8,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11285351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-typhoidal <i>Salmonella</i> in Nigeria: do outcomes of 'multisectoral' surveillance, treatment and control justify the intervention costs?","authors":"Abdullahi O Sanni, Abdurrahman H Jibril, Olubunmi G Fasanmi, Oluwawemimo O Adebowale, Alexander R Jambalang, Aminu Shittu, Annelize Jonker, Latifah O Abdulkarim, Folorunso O Fasina","doi":"10.1080/23144599.2024.2365567","DOIUrl":"10.1080/23144599.2024.2365567","url":null,"abstract":"<p><p>Non-typhoidal salmonellosis (NTS) is significant and an economic burden in Nigeria. To determine whether investment in NTS control is economically justifiable, Outbreak Costing Tool (OCT) was used to estimate the robust funding of public and animal health systems for epidemio-surveillance and control of multisectoral NTS outbreaks in Nigeria. Health, production, and economic data were collected and used to populate the tool for evaluation. The multisectoral NTS burden for the year 2020 in Nigeria was US$ 930,887,379.00. Approximately 4,835 technical officers, and 3,700 non-technical staff (<i>n</i> = 8,535) were needed with an investment of >2.2 million work hours. The investment cost for NTS control was US$ 53,854,660.87. The non-labour-related cost was 89.21% of the total intervention costs. The overall intervention's investment was 374.15% of the estimated national and subnational systems' annual budget for diarrhoeal diseases, and the outbreak response period attracted the highest costs (53%) of the total intervention. In conclusion, intervention against NTS was beneficial (benefit - cost ratio: 17.29), hence justifying the need for multisectoral surveillance-response against NTS in Nigeria. Complex sectoral silos must give way to coordinated collaborations to optimize benefits; and over-centralization of health interventions' associated delays must be removed through decentralized sub-national-focused framework that empowers rapid investigation, response, control, data collection, and analyses. It should assist anticipatory planning, and outbreak investigation and reduce critical response time. Anticipatory planning tools, when applied pre-emptively, can benefit budgeting, identify gaps, and assist in the delivery of cost-saving and effective measures against infectious disease.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"48-59"},"PeriodicalIF":2.8,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141621167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In field evaluation of impact on clinical signs of an inactivated autogenous vaccine against <i>Pasteurella multocida</i> in rabbits.","authors":"G Casalino, F D'Amico, G Bozzo, F R Dinardo, M Schiavitto, D Galante, A Aceti, E Ceci, D Romito, F P D'Onghia, M M Dimuccio, A Camarda, E Circella","doi":"10.1080/23144599.2024.2348900","DOIUrl":"10.1080/23144599.2024.2348900","url":null,"abstract":"<p><p>In Italy, the use of autogenous inactivated vaccines prepared with the bacterial strains isolated from affected animals is authorized by the Ministry of Health in farms where bacterial diseases occur frequently. The autogenous vaccine performed using <i>Pasteurella multocida</i> is frequently used in rabbit farms, but the feedback of its application is not available. Therefore, the aim of this study is to give information about the impact on the clinical signs of a bivalent autogenous vaccine in rabbits of a genetic centre. The vaccine was prepared using two <i>P. multocida</i> strains belonging to serogroups A and F, equipped with virulence genes and responsible for cyclical outbreak of pasteurellosis in the farm. The vaccine was administered with a first injection, followed by another one after 15 days, then another one four months after the first injection, and then continuing with a further injection every six months to all rabbits. Clinical conditions and mortality rates were monitored for two years after the first vaccination. The improvement in clinical condition and the decrease of the mortality rate were significant especially in the first year post-vaccine. In addition, the number of animals removed due to the disease decreased greatly. Based on the finding of <i>P. multocida</i> strains belonging to serogroup D and serogroup A equipped with different virulence-gene patterns from those previously found, we suggest that the vaccine was unable to prevent the introduction and spreading of new strains among the rabbits.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"39-47"},"PeriodicalIF":2.2,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11159587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141297002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptococcus iniae</i> in aquaculture: a review of pathogenesis, virulence, and antibiotic resistance.","authors":"María Zulema Juárez-Cortés, Luz Edith Casados Vázquez, Sergio Francisco Martínez Díaz, César Salvador Cardona Félix","doi":"10.1080/23144599.2024.2348408","DOIUrl":"10.1080/23144599.2024.2348408","url":null,"abstract":"<p><p>One of the main challenges in aquaculture is pathogenic bacterial control. <i>Streptococcus iniae</i> stands out for its ability to cause high mortality rates in populations of commercially important fish populations and its recent recognition as an emerging zoonotic pathogen. The rise in identifying over 80 strains some displaying antibiotic resistance coupled with the emerging occurrence of infections in marine mammal species and wild fish underscores the urgent need of understanding pathogenesis, virulence and drug resistance mechanisms of this bacterium. This understanding is crucial to ensure effective control strategies. In this context, the present review conducts a bibliometric analysis to examine research trends related to <i>S. iniae</i>, extending into the mechanisms of infection, virulence, drug resistance and control strategies, whose relevance is highlighted on vaccines and probiotics to strengthen the host immune system. Despite the advances in this field, the need for developing more efficient identification methods is evident, since they constitute the basis for accurate diagnosis and treatment.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"25-38"},"PeriodicalIF":2.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11095286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary-nucleotides and Saccharomyces cerevisiae-derivatives on growth-performance, antioxidant-capacity, immune-response, small-intestine histomorphometry, caecal-<i>Clostridia</i>, and litter-hygiene of broiler-chickens treated with florfenicol.","authors":"Tamer Mohamed Abo-Sriea, Elshaimaa Ismael, Basant Mohsen Sobhi, Neven H Hassan, Ebtihal M M Elleithy, Sherif AbdElmonam Omar, Ahmed M Soliman, Khaled Nasr El-Din Fahmy, Amer Ramadan","doi":"10.1080/23144599.2024.2324411","DOIUrl":"10.1080/23144599.2024.2324411","url":null,"abstract":"<p><p>Stress in poultry production is energy-demanding. Nucleotides and yeast cell-wall products are essential nutrients for broiler performance, gut function, and immune response. Antibiotics, like florfenicol, negatively affect the immune system. A total of 600 one-d-old broiler chickens (Cobb-500) were weighed and randomly allotted into four groups with three replicates each. The control group (G1) received the basal diet, G2 received a diet supplemented with a combination of nucleotides and <i>Saccharomyces cerevisiae</i> derivatives (250 g/Ton), G3 received the basal diet and medicated with florfenicol (25 mg/Kg body weight) in drinking water for 5 days, while G4 received a combination of nucleotides and <i>Saccharomyces cerevisiae</i>-derivatives (250 g/Ton) and medicated with florfenicol in drinking water. Growth performance criteria were recorded weekly. Blood, intestinal contents, small-intestine sections, and litter samples were collected to measure birds' performance, carcass yields, leukocytic counts, antioxidant capacity, antibody titres, phagocytic index, caecal <i>Clostridia</i>, intestinal histomorphometry, and litter hygiene. Nucleotide-supplemented groups (G2 and G4) revealed significant (<i>p</i> ≤ 0.05) improvements in feed conversion, and body weight, but not for carcass yields in comparison to the control. Dietary nucleotides in G2 elevated blood total proteins, leucocytic count, antioxidant capacity, and phagocytic index, while they lowered blood lipids and litter moisture and nitrogen (<i>p</i> ≤ 0.05). Dietary nucleotides in G4 ameliorated the immunosuppressive effect of florfenicol (<i>p</i> ≤ 0.05) indicated in reducing caecal <i>Clostridia</i>, improving duodenal and ileal villi length, and increasing blood albumin and globulin levels, and phagocytosis%. Supplementing diets with nucleotides and yeast products has improved the immune system and provided a healthier gut for broilers.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"11-24"},"PeriodicalIF":2.2,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10939105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breed and non-genetic risk factors associated with the prevalence of subclinical mastitis in livestock systems of Arauca, Colombian orinoquia.","authors":"Arcesio Salamanca-Carreño, Mauricio Vélez-Terranova, Diana Patricia Barajas-Pardo, Rita Tamasaukas, Raúl Jáuregui-Jiménez, Pere M Parés-Casanova","doi":"10.1080/23144599.2024.2310451","DOIUrl":"10.1080/23144599.2024.2310451","url":null,"abstract":"<p><p>Subclinical Mastitis (SCM) is caused by several factors associated with the interaction of the individual cow, cow management, and the environment. The aim of this study was to assess the breed and non-genetic risk factors on the prevalence of SCM in cows from the dual-purpose livestock system on the floodplain of Arauca, Colombian Orinoquia. Milk samples were taken from the individual mammary quarters of 481 cows representing 28 different farms where the electrical conductivity (EC) test applied. To determine the factors associated with SCM, a multiple logistic regression analysis was used. The response variable was the SCM presence (1), or absence (0) obtained with the EC test. Breed was included as a genetic risk factor, and as non- genetic risk factors: number of cows in production, daily milk production, lactation month, cow age, climatic period, body condition, and calving number. The factors that were significantly associated with the SCM presence were body condition, climatic period, and breed (<i>p</i> < 0.05). Odds ratio (OR) analysis of significant effects indicates that for each unit increase in body condition, the OR of having animals with SCM is reduced by 71%. In the dry period the OR of animals with SCM increases by 150% compared to the rainy period. Composite breeds reduce the OR of SCM animals by 73%, compared with <i>Indicus</i>-predominance animals. In this study, the occurrence of SCM in extensive management systems in Arauca, Colombian Orinoquia, is determined by the risk factors of breed, climatic period, and body condition.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"12 1","pages":"1-10"},"PeriodicalIF":2.2,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10916920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140060717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody detection against Kunitz-type protein in <i>Fasciola hepatica</i> experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA)","authors":"María Ahumada, Agustina Godino, Lorena Guasconi, Carla Deheza, Marilla Amaranto, Cesar Iván Pruzzo, Gabriel Vitulli-Moya, Laura Chiapello, María Elena Carrizo, José Luis Barra, Laura Cervi","doi":"10.1080/23144599.2023.2273678","DOIUrl":"https://doi.org/10.1080/23144599.2023.2273678","url":null,"abstract":"Fasciolosis is a parasitic disease considered as emerging and neglected by the WHO. Sheep are highly susceptible to this disease, and affected flocks experience decreased productivity due to increased mortality, and the reduced quality of their products, such as wool and meat. To effectively control this disease, reliable and early diagnosis is essential for making decisions regarding antiparasitic application and/or the removal of affected animals. Currently, the diagnosis of F. hepatica in sheep relies on the detection of parasite eggs in faeces, a method that becomes reliable from week 10 post-infection. Consequently, there is a need for earlier diagnostic tools based on immune response. However, obtaining antigens for antibody detection has proven to be difficult and expensive. The aim of this study was to evaluate members of the Kunitz protein family of F. hepatica expressed in the form of a fusion protein in the serological diagnosis of F. hepatica in sheep. The performance of three recombinant F. hepatica Kunitz-type inhibitors (FhKT1.1, FhKT1.3, and FhKT4) was compared with a synthetic Kunitz-type peptide (sFhKT) in sera from sheep experimentally infected with F. hepatica, using an ELISA. Of these, FhKT1.1 showed the most promising diagnostic indicators, exhibiting high precision and low cross-reactivity, and thus potential for standardized production. The results of our study demonstrated that the application of FhKT1.1 is a valuable tool for early-stage diagnosis of F. hepatica in sheep. Such an early diagnosis can aid in implementing timely interventions and effectively managing the disease in sheep populations.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"26 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134954195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}