Prevalence of Cryptosporidium parvum, Giardia duodenalis and Eimeria spp. in diarrhoeic suckling calves from north-western Spain and analysis of their interactions.

Abstract

Although Cryptosporidium parvum, Giardia duodenalis and some Eimeria species are frequently involved in neonatal calf diarrhoea (NCD), detailed studies on their interactions are scarce. Therefore, a cross-sectional study including faecal samples from 404 diarrhoeic calves aged 0-30 days was performed. Cryptosporidium oocysts and G. duodenalis cysts were detected by immunofluorescence antibody test and positive samples were molecularly characterized. Eimeria oocysts were microscopically detected using the modified McMaster technique and morphometric species identification was performed. Cryptosporidium infections (53.7%) predominated over those by G. duodenalis (12.4%) and Eimeria spp. (6.9%). Three Cryptosporidium species were identified: C. parvum (98.0%), C. bovis (1.0%) and C. ryanae (1.0%). Eleven C. parvum zoonotic subtypes were detected, with IIaA15G2R1 as the most common; only G. duodenalis assemblage E was identified. Moreover, 10 Eimeria species were found, being Eimeria ellipsoidalis (96.4%), Eimeria bovis (85.7%), and Eimeria zuernii (71.4%) predominant. A significant positive association between G. duodenalis and Eimeria spp. was detected as well as a negative association between C. parvum and Eimeria spp. Our results showed that C. parvum wasprevalent in diarrhoeic calves under 21 days of life; Eimeria spp. and G. duodenalis were especially common in animals in their fourth week of life. Concurrent infections increased with age. Moreover, the study also revealed potential public health risks since a noticeable percentage of animals were infected with C. parvum zoonotic subtypes. Further studies are needed for determining the role of these parasites in co-infections with other enteropathogens and their implications in the pathogenicity of NCD.