Bulletin of Tokyo Dental College最新文献_第3页

Exploratory Study of Growth of Circumference of Mandibular Fossa Adjacent to Petrous Portion of Temporal Bone Using Dried Skulls. 利用干燥头骨对颞骨石状部分邻近下颌窝圆周生长的探索性研究

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2024-03-07 Epub Date: 2024-02-15 DOI: 10.2209/tdcpublication.2023-0009

Hidetomo Hirouchi, Ryu Suzuki, Shinya Hanada, Yuki Takeuchi, Yuki Sugiyama, Takeshi Takayama, Katsuhiko Hayashi, Gen Murakami, Shinichi Abe

{"title":"Exploratory Study of Growth of Circumference of Mandibular Fossa Adjacent to Petrous Portion of Temporal Bone Using Dried Skulls.","authors":"Hidetomo Hirouchi, Ryu Suzuki, Shinya Hanada, Yuki Takeuchi, Yuki Sugiyama, Takeshi Takayama, Katsuhiko Hayashi, Gen Murakami, Shinichi Abe","doi":"10.2209/tdcpublication.2023-0009","DOIUrl":"10.2209/tdcpublication.2023-0009","url":null,"abstract":"The morphogenetic process of development of the circumference of the mandibular fossa during tooth eruption, which involves the replacement of deciduous teeth with permanent teeth, is strongly affected by occlusion. To the best of our knowledge, no studies have investigated the effect of occlusion on this process. This study investigated the morphogenetic process of development during tooth eruption using dried skulls harvested from Indian donors. The average distance between the ala-major-squamosa suture and the foramen ovale according to age group was as follows: 3.24 mm in the 8-month-old group and 8.92 mm in the adult group. The average distance between the ala-major-squamosa suture and the apex of the articular tubercle according to age groups was as follows: 10.38 mm in the 8-month-old group and 19.34 mm in the adult group. The average distance between the point of intersection of the petrosquamous fissure and petrotympanic fissure located on the perpendicular line drawn posteriorly from the shortest distance of the medio-lateral axis between the ala-major-squamosa suture and the apex of the articular tubercle according to age group was as follows: 9.68 mm in the 8-month-old group and 14.3 mm in the adult group. These results suggest that the mandibular fossa is strongly affected by load due to occlusion, unlike the growth of the neurocranium. This indicates that the effect of occlusion is a secondary element in the morphogenetic process of development of the circumference of the mandibular fossa.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"11-17"},"PeriodicalIF":0.5,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139736368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of Gingival Recession in Multiple Teeth Using Coronally Advanced Flap with Connective Tissue Graft: A Case Report. 冠状前移瓣联合结缔组织移植治疗多牙龈退缩1例。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2023-12-28 Epub Date: 2023-11-15 DOI: 10.2209/tdcpublication.2023-0003

Keiko Yamashita, Fumi Seshima, Atsushi Saito

{"title":"Treatment of Gingival Recession in Multiple Teeth Using Coronally Advanced Flap with Connective Tissue Graft: A Case Report.","authors":"Keiko Yamashita, Fumi Seshima, Atsushi Saito","doi":"10.2209/tdcpublication.2023-0003","DOIUrl":"10.2209/tdcpublication.2023-0003","url":null,"abstract":"This report describes a case of gingival recession in multiple teeth with severe dentin hypersensitivity (DH) in which treatment included periodontal plastic surgery. The patient was a 34-year-old woman presenting with the chief complaint of DH at gingivalrecession sites. The patient had undergone orthodontic treatment when she was 30 years old. An initial examination revealed that none of the sites showed a probing depth of ≥4 mm and 21% of sites bleeding on probing. The clinical diagnosis was plaque-induced gingivitis. Teeth #14, 16, 23, 25, 26, 34, 35, 45, and 46 showed gingival recession ranging from 1 to 4 mm. Gingival recession at #45 extended to the muco-gingival junction. No association with alveolar bone loss was observed in any of the interdental areas. Therefore, the sites presenting with gingival recession were classified as Miller Class I, except #45, which was classified as Class II. The periodontal phenotype was 'thin'. Based on the results of clinical examination and diagnosis, initial periodontal therapy (IP) consisting of oral hygiene instruction, supra-gingival scaling, application of a desensitizing agent, and composite resin restoration was performed. The Visual Analog Scale (VAS) score, which was used to assess degree of DH, showed only a minimal decrease, however, at post-IP. Subsequently, a modified coronally advanced tunnel (a modified technique for achieving a coronally advanced flap) using a connective tissue graft was performed in #14, 16, 23, 25, 26, 45, and 46. After re-evaluation, the patient was placed on maintenance care. The series of interventions resulted in a considerable improvement in the VAS and oral health-related quality of life scores. Furthermore, a change in the periodontal phenotype, from 'thin' to 'thick', was observed, which may contribute to the prevention of further gingival recession and DH. The present case suggests that periodontal plastic surgery is an effective treatment modality for the resolution of DH.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"125-133"},"PeriodicalIF":0.5,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periodontal Regenerative Therapy with Recombinant Human Fibroblast Growth Factor (rhFGF) -2 for Stage III Grade C Periodontitis: A Case Report with 6-month Follow-up. 重组人成纤维细胞生长因子(rhFGF) -2牙周再生治疗III期C级牙周炎:1例6个月随访报告

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2023-12-28 Epub Date: 2023-11-15 DOI: 10.2209/tdcpublication.2023-0018

Yoshimi Saito, Kentaro Imamura, Fumi Seshima, Toshikatsu Nakazaki, Sachiyo Tomita, Atsushi Saito

{"title":"Periodontal Regenerative Therapy with Recombinant Human Fibroblast Growth Factor (rhFGF) -2 for Stage III Grade C Periodontitis: A Case Report with 6-month Follow-up.","authors":"Yoshimi Saito, Kentaro Imamura, Fumi Seshima, Toshikatsu Nakazaki, Sachiyo Tomita, Atsushi Saito","doi":"10.2209/tdcpublication.2023-0018","DOIUrl":"10.2209/tdcpublication.2023-0018","url":null,"abstract":"This report describes a case of generalized chronic periodontitis requiring periodontal regenerative therapy. The patient was a 56-year-old woman visiting the Tokyo Dental College Suidobashi Hospital with the chief complaint of swelling in the maxillary right gingiva. An initial examination revealed 34.0% of sites with a probing depth (PD) of ≥4 mm. The prevalence of sites with bleeding on probing was 32.7%. The plaque control record (PCR) score was 65.7%. Radiographic examination revealed angular bone resorption at #18 and 48. Horizontal absorption was also observed in other areas. The percent bone loss/age at #48 was 1.07. A clinical diagnosis of generalized chronic periodontitis (Stage III, Grade C) was made. Based on the clinical diagnosis of severe chronic periodontitis, initial periodontal therapy was performed. An improvement was observed in periodontal conditions at re-evaluation. The PCR score was 16.7%. Periodontal surgery was performed for teeth with a residual PD of ≥4 mm. Periodontal regenerative therapy using rhFGF-2 were performed on intrabony defects in #18 and 48. Open flap debridement was performed on #16, 26, and 27. Following evaluation, oral function was restored using all-ceramic crowns (#46). At 6 months postoperatively, the patient was transitioned to supportive periodontal therapy (SPT). During the 6-month SPT, stable periodontal conditions that facilitated a favourable level of plaque control were maintained.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"135-144"},"PeriodicalIF":0.5,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Case Report of Periodontal Regenerative Therapy Using Recombinant Human Fibroblast Growth Factor 2 and Deproteinized Bovine Bone Mineral with Non-incised Papillae Surgical Approach (NIPSA) for Angular Bone Defect in Patient with Stage III Grade C Periodontitis. 重组人成纤维细胞生长因子2和脱蛋白牛骨矿物质联合非切开乳头手术治疗III期C级牙周炎角状骨缺损1例报告。

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Bulletin of Tokyo Dental College Pub Date : 2023-12-28 Epub Date: 2023-11-15 DOI: 10.2209/tdcpublication.2023-0011

Takahiro Bizenjima, Daisuke Irokawa, Shigeko Yamada, Atsushi Saito, Sachiyo Tomita

{"title":"A Case Report of Periodontal Regenerative Therapy Using Recombinant Human Fibroblast Growth Factor 2 and Deproteinized Bovine Bone Mineral with Non-incised Papillae Surgical Approach (NIPSA) for Angular Bone Defect in Patient with Stage III Grade C Periodontitis.","authors":"Takahiro Bizenjima, Daisuke Irokawa, Shigeko Yamada, Atsushi Saito, Sachiyo Tomita","doi":"10.2209/tdcpublication.2023-0011","DOIUrl":"10.2209/tdcpublication.2023-0011","url":null,"abstract":"This report describes a case of Stage III Grade C periodontitis requiring periodontal regenerative therapy. The patient was a 19-year-old woman who presented with the chief complaint of gingival recession in the incisor region. An initial examination revealed that 45.3% of sites had a probing depth of ≥4 mm and 45.8% bleeding on probing. Radiographic examination showed angular bone resorption in #25, 26, 31, 36, and 46 and horizontal resorption in other regions. Initial periodontal therapy was implemented based on a clinical diagnosis of Stage III Grade C periodontitis (generalized aggressive periodontitis). Occlusal adjustment was also performed at sites showing premature contact (#26 and 36) after suppression of inflammation. Periodontal regenerative therapy using recombinant human fibroblast growth factor (rhFGF) -2 was performed on #25, 26, and 46. Combination therapy with rhFGF-2 and deproteinized bovine bone mineral (DBBM) was performed on #31 and 36. A non-incised papillae surgical approach (NIPSA) was used on #31. Periodontal conditions were then re-evaluated and the patient placed on supportive periodontal therapy. Regenerative therapy using rhFGF-2 and DBBM with NIPSA yielded an improvement in clinical parameters and bone resorption. This improvement has been adequately maintained over a 12-month period. Continued care is needed to maintain stable periodontal conditions.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"145-155"},"PeriodicalIF":0.5,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional Maxillofacial Morphology Measurements in Japanese Adults with Normal Occlusion. 日本成人正常咬合的三维颌面形态学测量。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2023-12-28 Epub Date: 2023-11-15 DOI: 10.2209/tdcpublication.2023-0012

Mai Takusagawa, Yasushi Nishii, Kunihiko Nojima, Shinichi Abe, Takashi Takaki, Kenji Sueishi

{"title":"Three-dimensional Maxillofacial Morphology Measurements in Japanese Adults with Normal Occlusion.","authors":"Mai Takusagawa, Yasushi Nishii, Kunihiko Nojima, Shinichi Abe, Takashi Takaki, Kenji Sueishi","doi":"10.2209/tdcpublication.2023-0012","DOIUrl":"10.2209/tdcpublication.2023-0012","url":null,"abstract":"Accurate orthodontic analysis and diagnosis based on anatomical landmarks is essential to the success of orthodontic treatment. Helical computed tomography (CT) has evolved markedly, and dentists can now quickly obtain 3-dimensional (3D) reconstruction data using this imaging modality. The planning of orthodontic treatment had traditionally been based on cephalometric analysis using 2D landmarks. This study aimed to collect 3D morphological data using CT images to establish new landmarks for analysis and diagnosis in orthodontic treatment. Twenty male and 20 female adult Japanese dry skulls with of normal occlusion were selected. The skulls were scanned using a multidetector helical CT system (SIEMENS, Volume Zoom Plus 4, Germany). Models were reconstructed using 3D measurement software (Simplant, Dentsply Sirona, Tokyo, Japan) and 45 landmarks determined. Three-dimensional measurement for a total of 30 items representing these landmarks was then performed. The results provided 3D standard values for maxillofacial morphology in adult Japanese individuals with normal occlusion. These measurement items should allow the disadvantages of 2D cephalometric analysis to be overcome.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"115-124"},"PeriodicalIF":0.5,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct Mechanical Stimulation Mediates Cell-to-Cell Interactions in Cultured Trigeminal Ganglion Cells. 直接机械刺激介导三叉神经节细胞间相互作用。

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Bulletin of Tokyo Dental College Pub Date : 2022-09-05 Epub Date: 2022-08-15 DOI: 10.2209/tdcpublication.2021-0034

Tatsuhiko Yazaki, Hidetaka Kuroda, Maki Kimura, Sadao Ohyama, Tatsuya Ichinohe, Yoshiyuki Shibukawa

{"title":"Direct Mechanical Stimulation Mediates Cell-to-Cell Interactions in Cultured Trigeminal Ganglion Cells.","authors":"Tatsuhiko Yazaki, Hidetaka Kuroda, Maki Kimura, Sadao Ohyama, Tatsuya Ichinohe, Yoshiyuki Shibukawa","doi":"10.2209/tdcpublication.2021-0034","DOIUrl":"https://doi.org/10.2209/tdcpublication.2021-0034","url":null,"abstract":"Trigeminal neuralgia occurs in the orofacial region, characteristically causing pain that feels like a transient electric shock. Some histopathological studies have reported that trigeminal neuralgia is caused by mechanical compression of the demyelinated trigeminal nerve; the pathophysiological mechanism behind this phenomenon remains to be clarified, however. Cell-cell interactions have also been reported to be involved in the development and modulation of some types of neuropathic pain. The purpose of this study was to investigate the potential contribution of cell-cell interactions to trigeminal neuralgia by measuring intracellular free Ca2+ concentrations ([Ca2+]i) in primary cultured trigeminal ganglion (TG) cells. Direct mechanical stimulation of TG cells induced an increase in [Ca2+]i in both neuronal and non-neuronal cells, such as glial cells. Moreover, this increase was stimulus intensity-dependent and non-desensitizing. Direct mechanical stimulation increased [Ca2+]i in neighboring cells as well, and this increase was inhibited by application of carbamazepine. These results indicate that direct mechanical stimulation affects Ca2+ signaling. Trigeminal ganglion cells establish intercellular networks between themselves, suggesting that this is involved in the development and generation of trigeminal neuralgia.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"63 3","pages":"109-117"},"PeriodicalIF":0.5,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuropathic Pain in Lower Lip after Guided Tissue Regeneration: A Case Report. 引导组织再生后下唇的神经性疼痛：一例报告。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2022-09-05 Epub Date: 2022-08-15 DOI: 10.2209/tdcpublication.2022-0007

Toshiyuki Handa, Tatsuya Ichinohe

{"title":"Neuropathic Pain in Lower Lip after Guided Tissue Regeneration: A Case Report.","authors":"Toshiyuki Handa, Tatsuya Ichinohe","doi":"10.2209/tdcpublication.2022-0007","DOIUrl":"https://doi.org/10.2209/tdcpublication.2022-0007","url":null,"abstract":"Post-traumatic trigeminal neuropathic pain is mainly caused by the extraction of third molars or the placement of dental implants. This report describes the treatment of neuropathic pain arising after guided tissue regeneration (GTR). The patient was a 55-year-old woman who had to undergo GTR due to severe periodontitis in the distal aspect of the right mandibular second molar. Postoperatively, the patient had been prescribed mecobalamin for hypesthesia and allodynia in the right lower lip. No improvement was observed in these symptoms after 4 months, however, so she was referred to our Orofacial Pain Center. Preoperative and postoperative cone-beam computed tomography revealed a cyst-like lesion (radiolucent area) close to the right mandibular second molar and canal. Although the results of quantitative sensory examination were normal, rubbing the right lower lip with a cotton swab elicited mechanical allodynia. The diagnosis was post-traumatic trigeminal neuropathic pain for which the patient was given pregabalin and Neurotropin®. The symptoms improved within approximately 32 weeks, with the medication being terminated at 64 weeks. Although hypoesthesia due to nerve injury may suddenly go into remission, allodynia is often intractable. If symptoms show no improvement after 3 months, possible nerve injury should be investigated. Additionally, the distal root of the mandibular molar may be close to the inferior alveolar nerve, necessitating appropriate diagnostic imaging of the operative field. If the lesion or distal root is close to the inferior alveolar nerve, postoperative hypesthesia or neuropathic pain may occur, even without direct trauma.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"63 3","pages":"139-144"},"PeriodicalIF":0.5,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Surgical Periodontal Therapy Using Recombinant Human Fibroblast Growth Factor-2 in Combination with Carbonate Apatite Granules for Stage III Grade B Periodontitis: A Case Report with 1-year Follow-up. 重组人成纤维细胞生长因子-2联合碳酸钙磷灰石颗粒治疗III期B级牙周炎:1例1年随访报告。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2022-09-05 Epub Date: 2022-08-15 DOI: 10.2209/tdcpublication.2022-0008

Tasuku Murakami, Kentaro Imamura, Naoko Watanabe-Egawa, Sachiyo Tomita, Atsushi Saito

{"title":"Surgical Periodontal Therapy Using Recombinant Human Fibroblast Growth Factor-2 in Combination with Carbonate Apatite Granules for Stage III Grade B Periodontitis: A Case Report with 1-year Follow-up.","authors":"Tasuku Murakami, Kentaro Imamura, Naoko Watanabe-Egawa, Sachiyo Tomita, Atsushi Saito","doi":"10.2209/tdcpublication.2022-0008","DOIUrl":"https://doi.org/10.2209/tdcpublication.2022-0008","url":null,"abstract":"This report describes a case of generalized chronic periodontitis requiring periodontal treatment including regenerative therapy. The patient was a 60-year-old woman who visited the Tokyo Dental College Suidobashi Hospital with the chief complaint of tooth mobility and pain in tooth #26. Periodontal examination at the first visit revealed that 32.0% of sites had a probing depth of ≥4 mm and 43.8% bleeding on probing. Radiographic examination revealed vertical bone resorption in #17. Horizontal resorption was noted in other areas. Initial periodontal therapy consisting of plaque control, scaling and root planing, and caries treatment was performed based on a clinical diagnosis of Stage III Grade B periodontitis. Tooth #26 was extracted due to bone resorption extending as far as the root apex. After reevaluation, periodontal regenerative therapy using recombinant human fibroblast growth factor-2 (rhFGF-2) in combination with carbonate apatite (CO3Ap) granules was performed for #17. Following reevaluation, a zirconia crown (#16) and zirconia bridge (#24-27) were placed. Following further reevaluation, the patient was placed on supportive periodontal therapy (SPT). The periodontal regenerative therapy using rhFGF-2 with CO3Ap granules yielded an improvement in the vertical bone resorption observed in #17. This improvement has been adequately maintained over a 1-year period postoperatively. Continued SPT is needed to maintain stable periodontal conditions.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"63 3","pages":"145-153"},"PeriodicalIF":0.5,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Novel Regulator Involved in Differentiation of Mouse iPS Cells into Odontoblast-like Cells. 小鼠iPS细胞向成牙细胞样细胞分化的新调控因子的鉴定。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2022-09-05 Epub Date: 2022-08-15 DOI: 10.2209/tdcpublication.2021-0045

Kenji Inoue, Kenichi Matsuzaka, Takashi Inoue

{"title":"Identification of Novel Regulator Involved in Differentiation of Mouse iPS Cells into Odontoblast-like Cells.","authors":"Kenji Inoue, Kenichi Matsuzaka, Takashi Inoue","doi":"10.2209/tdcpublication.2021-0045","DOIUrl":"https://doi.org/10.2209/tdcpublication.2021-0045","url":null,"abstract":"Odontoblasts differentiate from dental papilla stem cells, but the genetic changes that occur during this process remain unclear. The aim of this study was to investigate gene expression patterns during differentiation of mouse iPS cells into odontoblast-like cells. Mouse iPS cells were cultured on a collagen type-1 scaffold with bone morphogenetic protein 4 (BMP4) and retinoic acid (RA). The results of immunofluorescence studies for dentin sialoprotein, dentin matrix protein 1 (DMP1), and nestin were positive. A qRT-PCR analysis revealed that mRNA expression levels of neural crest marker sex determining region Y box (Sox)-10, dentin sialophosphoprotein (Dspp), and Dmp1 were up-regulated, but that mRNA expression levels of the mineralization markers bone sialoprotein and osteocalcin were down-regulated. Microarray analysis showed that 2,597 entities were up-regulated and 1,327 down-regulated among a total of 15,330 investigated. Sox11 was among the up-regulated genes identified. The Sox11 mRNA expression level with odontoblast induction after day 11 was higher than that after day 2 (p<0.05). Gene knockdown using small interference RNA (siRNA) silencing was used to characterize the function of Sox11. The Dspp mRNA expression level in Sox11 siRNA-treated cells was significantly lower than that in the control (p<0.05). These results suggest that BMP4 and RA induce mouse iPS cells to differentiate into odontoblast-like cells. The differentiation efficiency is not high, however, and many stem cells remain. The results also suggest that Sox11 is an important factor in odontoblastic differentiation.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"63 3","pages":"119-128"},"PeriodicalIF":0.5,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40412085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

External Root Resorption of Second Molars Due to Impacted Mandibular Third Molars during Orthodontic Retention. 正畸固位时下颌第三磨牙阻生对第二磨牙外牙根吸收的影响。

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Bulletin of Tokyo Dental College Pub Date : 2022-09-05 Epub Date: 2022-08-15 DOI: 10.2209/tdcpublication.2021-0044

Dai Ariizumi, Teruo Sakamoto, Masae Yamamoto, Yasushi Nishii

引用次数: 1