Bulletin of Tokyo Dental College最新文献_第10页

Comparison of the activity of four chitosan derivatives in reducing initial adherence of oral bacteria onto tooth surfaces. 四种壳聚糖衍生物降低口腔细菌在牙齿表面的初始粘附活性的比较。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-11-01 DOI: 10.2209/TDCPUBLICATION.42.243

H. Sano, K. Shibasaki, T. Matsukubo, Y. Takaesu

{"title":"Comparison of the activity of four chitosan derivatives in reducing initial adherence of oral bacteria onto tooth surfaces.","authors":"H. Sano, K. Shibasaki, T. Matsukubo, Y. Takaesu","doi":"10.2209/TDCPUBLICATION.42.243","DOIUrl":"https://doi.org/10.2209/TDCPUBLICATION.42.243","url":null,"abstract":"We examined the effects of four kinds of chitosan derivatives on initial adherence of oral bacteria onto human anterior teeth surfaces. The buccal surfaces of anterior teeth were used as the experimental surfaces. They were divided into five rectangle areas with outer dimensions of about 2 mm x 4 mm. After applying two ml of a sample solution onto the tooth surfaces, an examiner wiped each rectangle area with a sterilized plastic swab one, three and six hours later. Then we measured bacterial counts in sterilized swabs with mitis salivarius agar. We found that the order of magnitude of the inhibitory effect on the adherence of oral bacteria was low molecular chitosan > phosphorylated chitosan > amorphous chitosan > carboxymethyl chitosan. The solution containing 0.5% low molecular chitosan depressed the bacterial adherence to the same extent as a 50 ppm chlorhexidine digluconate solution for three hours, and 0.1% phosphorylated chitosan also exhibited an inhibitory effect in bacterial adherence for one hour. Amorphous chitosan had a moderate inhibitory effect, but no clear inhibitory activity was found with 0.1% carboxymethyl chitosan. These results suggest that low molecular chitosan and phosphorylated chitosan have the potential to effectively inhibit the initial adherence of oral bacteria onto human tooth surfaces.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"34 1","pages":"243-9"},"PeriodicalIF":0.5,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2209/TDCPUBLICATION.42.243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68186478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Measurement of pulpal blood flow in dogs with nonradioactive colored microspheres. 用非放射性有色微球测量犬髓血流量。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-11-01 DOI: 10.2209/TDCPUBLICATION.42.201

T. Tanaka, Y. Kaneko

{"title":"Measurement of pulpal blood flow in dogs with nonradioactive colored microspheres.","authors":"T. Tanaka, Y. Kaneko","doi":"10.2209/TDCPUBLICATION.42.201","DOIUrl":"https://doi.org/10.2209/TDCPUBLICATION.42.201","url":null,"abstract":"The viability of dental pulp depends largely on regional blood flow, as in other organs. Measurement of absolute pulpal blood flow (PBF) and comparisons with blood flow in other organs allow the prediction of microvascular regulation in dental pulp. In previous studies, PBF was measured in dogs mainly with radioactive microspheres. However, this established technique is inaccessible to many investigators due to concerns over radiation safety and radioactive waste. To overcome these limitations, a new method has been introduced that involves the use of nonradioactive colored microspheres for measuring regional blood flow in the myocardium and in other organs in animals. However, no previous studies have investigated the use of this method to measure PBF in dogs. We attempted to determine whether blood flow in dental pulp, which comprises a small amount of the total tissue in dogs, could be measured using this technique by comparing the measured values with those for regional myocardial blood flow. Mean blood flow values were between 0.148 and 0.182 ml/min/g for dental pulp at four different sites and about 1.0 ml/min/g in regional myocardium. These values are comparable to those previously reported using radioactive microspheres. As nonradioactive colored microspheres safety permitted measurement of absolute PBF in dogs, this technique appears to be useful for research into microvascular blood flow in dental pulp.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"42 4 1","pages":"201-10"},"PeriodicalIF":0.5,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68186684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Allelic imbalance on the long arm of chromosome 21 in human oral squamous cell carcinoma: relationship between allelic imbalances (LOH and MSI) and clinicopathologic features. 人口腔鳞状细胞癌21号染色体长臂上的等位基因失衡:等位基因失衡(LOH和MSI)与临床病理特征的关系

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-11-01 DOI: 10.2209/TDCPUBLICATION.42.211

N. Yamamoto, H. Noma, T. Shibahara

{"title":"Allelic imbalance on the long arm of chromosome 21 in human oral squamous cell carcinoma: relationship between allelic imbalances (LOH and MSI) and clinicopathologic features.","authors":"N. Yamamoto, H. Noma, T. Shibahara","doi":"10.2209/TDCPUBLICATION.42.211","DOIUrl":"https://doi.org/10.2209/TDCPUBLICATION.42.211","url":null,"abstract":"Frequent allelic imbalances, including loss of heterozygosity (LOH) and microsatellite instability (MSI), have been found on the long arm of chromosome 21 (21q) in several types of human cancer. This study was designed to identify the tumor suppressor locus (or loci) associated with oral squamous cell carcinoma (SCC) on 21q. In order to understand the details of genetic alterations on chromosome 21, we performed polymerase chain reaction analysis of microsatellite polymorphisms corresponding to ten loci on this chromosome. We examined forty primary tumor tissues, forty corresponding normal tissues, and seven lymph node metastatic tissues. We identified novel tumor suppressor loci in this region in primary oral SCCs. To further determine the role of 21q deletions in oral cavity carcinogenesis, forty oral SCCs were examined for allelic imbalances (LOH or MSI) at 21q using ten microsatellite markers. Among these forty patients, twenty-six (65%) showed LOH at one or more loci. Deletion mapping of these tumors revealed four discrete, commonly deleted regions on the chromosome arm. Furthermore, we detected MSI in seventeen of those tested cases (42.5%). We compared our results with the clinicopathologic features. A number of sites displaying LOH at 21q could be detected in early stage lesions, and the frequencies of LOH tended to be higher in later clinical stages, but no statistical correlation was observed. Our results strongly suggest that allelic imbalances on 21q are involved in the development of oral SCC and that at least four different putative tumor suppressor genes contributing to the pathogenesis of this disease are present on 21q. Furthermore, allelic loss on 21q appears to be a useful indicator for evaluating the malignancy and prognosis of oral SCC, because the LOH of recurrent cases was more frequent than that of non-recurrent ones.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"42 4 1","pages":"211-23"},"PeriodicalIF":0.5,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2209/TDCPUBLICATION.42.211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68186412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Characteristics of newly formed bone during guided bone regeneration: observations by immunohistochemistry and confocal laser scanning microscopy. 引导骨再生过程中新生骨的特征:免疫组织化学和共聚焦激光扫描显微镜观察。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-11-01 DOI: 10.2209/TDCPUBLICATION.42.225

K. Matsuzaka, M. Shimono, T. Inoue

{"title":"Characteristics of newly formed bone during guided bone regeneration: observations by immunohistochemistry and confocal laser scanning microscopy.","authors":"K. Matsuzaka, M. Shimono, T. Inoue","doi":"10.2209/TDCPUBLICATION.42.225","DOIUrl":"https://doi.org/10.2209/TDCPUBLICATION.42.225","url":null,"abstract":"The purpose of this study was to investigate the characteristics of new bone formation during guided bone regeneration (GBR) using immunohistochemistry and confocal laser scanning microscopy. e-PTFE membranes were applied to defects created in the tibiae of rats, and some animals were sacrificed 6, 8, or 10 days later. Serial paraffin sections were cut, stained with H-E, and examined to analyze the ratio of new bone formation. Immunohistochemical staining with a monoclonal antibody specific for PCNA was used to evaluate the proliferating activity. In other experimental rats, calcein was injected at 6, 8, and 10 days after the surgery, and the animals were sacrificed 48 hr after injection. Their tibiae were removed, and Villanueva bone staining was performed before observation using confocal laser scanning microscopy to investigate the mineralization of new bones. The bone occupation ratio increased day by day, but the experimental groups had significantly higher ratios than control groups (without membrane) at each of the time periods. However, PCNA positive cells decreased over time in all groups, and there were no significant differences among the groups. Mineralization occurred more rapidly in the experimental groups than in the control groups. These results suggest that GBR accelerates the migration of osteogenic cells, the formation of new bone, and mineralization in the defect created by the e-PTFE membrane.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"42 4 1","pages":"225-34"},"PeriodicalIF":0.5,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2209/TDCPUBLICATION.42.225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68186422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Decay in prepulse facilitation of calcium channel currents by Gi/o-protein attenuation in hamster submandibular ganglion neurons, but not Gq/11. Gi/o蛋白衰减对鼠下颌骨神经节神经元钙通道电流脉冲前易化的影响，但对Gq/11无影响。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-11-01 DOI: 10.2209/TDCPUBLICATION.42.235

T. Endoh, M. Abe, T. Suzuki

{"title":"Decay in prepulse facilitation of calcium channel currents by Gi/o-protein attenuation in hamster submandibular ganglion neurons, but not Gq/11.","authors":"T. Endoh, M. Abe, T. Suzuki","doi":"10.2209/TDCPUBLICATION.42.235","DOIUrl":"https://doi.org/10.2209/TDCPUBLICATION.42.235","url":null,"abstract":"The calcium ion influx through voltage-dependent calcium channels (VDCCs) has a vital role in the control of neurotransmitter release and membrane excitability. Prepulse facilitation is a phenomenon in which a strong depolarizing pulse induces a form of the VDCCs that exhibits an increased opening probability in response to a given test potential; this persists for several seconds after repolarization. It has been reported that prepulse facilitation occurs via dissociation of the guanosine triphosphate (GTP)-binding proteins (G-proteins) from the VDCCs and that recovery from facilitation involves rebinding of the G-proteins. The heterotrimeric G-proteins act as switches that regulate information processing circuits connecting cell surface G-protein-coupled-receptors to a variety of effectors. In this study, we have studied the characterization of G-protein subtypes in prepulse facilitation of VDCCs currents (Ica) in hamster submandibular ganglion (SMG) neurons, using whole-cell patch clamp recordings. Under control conditions, with GTP (0.1 mM) in the recording pipette, the rate of prepulse facilitation was 19.0 +/- 1.9% (n = 13). Intracellular dialysis with GDP-beta-S (0.1 mM), G-protein blocker, and pretreatment of neurons with N-ethylmaleimide (NEM) (100 microM for 2 min), Gi/o blocker, attenuated the rate of prepulse facilitation. Intracellular dialysis of anti-Gq/11-antibody did not alter it. These results suggest that prepulse facilitation of VDCCs is due to Gi/o-types of G-protein, but not to the Gq/11-type, in SMG neurons.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"61 1","pages":"235-41"},"PeriodicalIF":0.5,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68186431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Frequent allelic loss/imbalance on the short arm of chromosome 3 in tongue cancer. 舌癌患者3号染色体短臂上常见的等位基因丢失/不平衡。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-08-01 DOI: 10.2209/TDCPUBLICATION.42.151

K. Arai, T. Shibahara, N. Yamamoto, T. Yakushiji, C. Tanaka, H. Noma

引用次数: 14

Combination therapy including pentoxifylline for entero-Behçet's disease. 包括己酮可可碱在内的联合治疗entero- behaperet病。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-08-01 DOI: 10.2209/TDCPUBLICATION.42.169

T. Hisamatsu, H. Kishikawa, H. Fukuya, K. Matsuoka, Y. Mizuno, J. Nishida

引用次数: 8

Kinetic analysis of prepulse facilitation of calcium currents in hamster submandibular ganglion neurons. 仓鼠下颌下神经节神经元钙电流脉前易化的动力学分析。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-08-01 DOI: 10.2209/TDCPUBLICATION.42.185

T. Endoh, E. Yamada, T. Suzuki

引用次数: 2

The use of an osseointegrated implant for orthodontic anchorage to a Class II Div 1 malocclusion. 使用骨整合种植体矫治II类Div 1错牙合。

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-08-01 DOI: 10.2209/TDCPUBLICATION.42.177

K. Nojima, K. Komatsu, Y. Isshiki, H. Ikumoto, J. Hanai, C. Saito

引用次数: 9

Analysis of a role for p16/CDKN2 expression and methylation patterns in human oral squamous cell carcinoma. p16/CDKN2表达和甲基化模式在人口腔鳞状细胞癌中的作用分析

IF 0.5

Bulletin of Tokyo Dental College Pub Date : 2001-08-01 DOI: 10.2209/TDCPUBLICATION.42.159

T. Yakushiji, H. Noma, T. Shibahara, K. Arai, N. Yamamoto, C. Tanaka, K. Uzawa, H. Tanzawa

{"title":"Analysis of a role for p16/CDKN2 expression and methylation patterns in human oral squamous cell carcinoma.","authors":"T. Yakushiji, H. Noma, T. Shibahara, K. Arai, N. Yamamoto, C. Tanaka, K. Uzawa, H. Tanzawa","doi":"10.2209/TDCPUBLICATION.42.159","DOIUrl":"https://doi.org/10.2209/TDCPUBLICATION.42.159","url":null,"abstract":"The p16/CDKN2 (cyclin dependent kinase number 2) gene is known to be one of the negative regulators of the cell cycle. Aberrant 5'CpG island methylation is one of the most important mechanisms of p16/CDKN2 gene promoter region alteration. We studied 8 oral squamous cell carcinoma cell lines and 25 primary tumor tissues for the p16/CDKN2 gene and its expression by PCR-SSCP, MSP, RT-PCR, and immunohistochemical methods to determine the mechanism and the potential biological significance of p16/CDKN2 gene inactivation. In primary tumors, no p16/CDKN2 gene mutations were found by PCR-SSCP. However, hypermethylation of the CpG sites of p16/CDKN2 gene was observed in 48% (12/25) cases of primary tumors and in 50% (4/8) of cell lines. To verify the p16 mRNA expression, we employed RT-PCR and observed decreased or lacked p16 mRNA in 44% (11/25) of primary tumor tissues. In addition, hypermethylation was observed in 6 of the above 11 cases (55%). An immunohistochemistry assay was also performed with the primary tumor tissues, and a semi-quantitative method was used to evaluate the staining intensity of p16 protein. We observed 52% (13/25) negative nuclear staining. When we compared these results with clinicopathological stages, there was no statistical significance. These findings suggest that hypermethylation of p16/CDKN2 promoter region may be associated with p16/CDKN2 gene alteration.","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":"42 3 1","pages":"159-68"},"PeriodicalIF":0.5,"publicationDate":"2001-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68186160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21