Bulletin of Tokyo Dental College最新文献

{"title":"Remimazolam Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Macrophages.","authors":"Nobuyuki Matsuura, Yuka Sendai, Minami Hasegawa, Mio Fukada, Natsuko Aida, Shoko Onodera, Kyotaro Koshika, Tatsukuni Ohno","doi":"10.2209/tdcpublication.2025-0003","DOIUrl":"10.2209/tdcpublication.2025-0003","url":null,"abstract":"<p><p>Remimazolam (Re) is an ultra-short-acting benzodiazepine used for intravenous sedation, general anesthesia, and intensive care unit sedation. Although benzodiazepines, including midazolam, possess anti-inflammatory properties and suppress macrophage activity, the impact of Re on macrophage-mediated immune responses remains uncertain. The purpose of this study was to investigate the effects of Re on lipopolysaccharide (LPS)-stimulated macrophage activation using thioglycolate-induced mouse peritoneal macrophages (TGC-macrophages). The findings demonstrated that Re significantly attenuated the production of proinflammatory cytokines, tumor necrosis factor α, and interleukin-6 by LPS-stimulated TGC-macrophages. Notably, this inhibitory effect on cytokine production remained unaffected by flumazenil, a specific antagonist of the γ-aminobutyric acid type A (GABAA), indicating that Re exerts its anti-inflammatory effects on macrophages through a mechanism independent of GABAA receptor signaling. In Re-treated macrophages, the expression levels of antigen presentation-related molecules, including CD86, a costimulatory molecule, and major histocompatibility complex class II, were significantly reduced following LPS stimulation. Conversely, Re did not affect the phagocytic capacity of TGC-macrophages, as evaluated by fluorescein isothiocyanate-labeled dextran, even at concentrations that inhibited inflammatory cytokine production and costimulatory molecule expression in LPS-activated TGC-macrophages. These findings suggest that Re exerts anti-inflammatory effects without suppressing essential innate immune functions, such as phagocytosis by macrophages.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"1-10"},"PeriodicalIF":0.5,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Impaired Fasting or Diabetes Compared with That of Smoking Habit on Tooth Loss.","authors":"Hideyuki Kamijo, Atsushi Oyama, Seitaro Suzuki, Yuki Onose, Takako Eguchi, Koichi Yoshino, Atsushi Takayanagi, Yoshikazu Takahashi, Naoki Sugihara","doi":"10.2209/tdcpublication.2024-0058","DOIUrl":"10.2209/tdcpublication.2024-0058","url":null,"abstract":"<p><p>The purpose of this study was to investigate the impacts of diabetes and smoking on the risk of tooth loss in adults and the elderly. Dental receipts and specific health checkup data were used to assess increase in tooth extraction among people with a high blood glucose level or a smoking habit. The data were obtained from a database maintained by DeSC Healthcare Corporation, which supports the integrity of data held by health insurers. Dental receipts and specific health checkup data from 2018 to 2021 were used to obtain information on fasting blood glucose levels, smoking habits, and tooth extraction in 12,219 individuals aged 45-69 years. The results showed that the proportion of tooth extractions between 2018 and 2021 (32.3%) was higher in those with hyperglycemia. The proportion of tooth extractions in smokers (34.0%) was also significantly higher than that in non-smokers (21.6%). These results suggest that measures must be taken to promote dental and oral health care in those with hyperglycemia and in those with a smoking habit.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"27-32"},"PeriodicalIF":0.5,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-syndromic Bilateral Posterior Maxillary Supernumerary Teeth: Rare Combination of Paramolar and Distomolar.","authors":"Yunosuke Ikeda, Atsushi Ogawa, Noriaki Takahashi, Hiroyuki Yamada","doi":"10.2209/tdcpublication.2025-0016","DOIUrl":"10.2209/tdcpublication.2025-0016","url":null,"abstract":"<p><p>When teeth or odontogenic structures are present in excess of the normal number in the dental arch they are referred to as supernumerary. This paper describes a rare case of a maxillary right distomolar and left paramolar in an otherwise non-syndromic individual. The patient was a 23-year-old Japanese man who presented at our clinic seeking periodontal treatment. Intraoral examination revealed supernumerary teeth in the maxilla, and an extraction plan including the wisdom teeth was developed.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"11-15"},"PeriodicalIF":0.5,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term Oral Management for 2q37 Deletion Syndrome Patient.","authors":"Hiromi Homma, Atsuo Sakurai, Dai Ariizumi, Yasushi Nishii, Seikou Shintani","doi":"10.2209/tdcpublication.2025-0022","DOIUrl":"10.2209/tdcpublication.2025-0022","url":null,"abstract":"<p><p>2q37 deletion syndrome is an extremely rare chromosomal disorder, with approximately 150 cases reported worldwide. Affected individuals are known to have multisystem involvement, though dental findings associated with this syndrome remain poorly documented. This report describes the long-term dental management and oral characteristics of a pediatric patient with 2q37 deletion syndrome. The patient was an 11-year 2-monthold boy who presented with the chief complaints of dental caries and maxillary anterior crowding. Similarly to in previous reports regarding this syndrome, a clinical examination revealed short stature (120 cm, approximately 3 standard deviations below the mean), intellectual disability, tetralogy of Fallot, and habitual vomiting. Intraoral findings included 6 carious teeth, a high-arched palate, a constricted maxillary arch, and severe anterior crowding. Mild dental erosion associated with habitual vomiting was also noted. The initial dental treatment addressed the caries in the maxillary first molars and anterior teeth. Due to limited cooperation from the patient, orthodontic treatment was deferred, though regular follow-up examinations were conducted over a period of 7 years. Caregiver motivation remained high and patient cooperation improved, and sectional orthodontic treatment was initiated when he reached the age of 18 years 2 months. Throughout the approximately 10-year period of dental care, the mother of the patient, serving as caregiver, has remained consistently engaged, allowing for routine dental management to be maintained. No new carious lesions have developed during this period and notable improvement in anterior alignment has been achieved. Long-term follow-up will be continued.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"17-25"},"PeriodicalIF":0.5,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146203348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SigH of Porphyromonas gingivalis Regulates Oxidative Stress Resistance and Invasion of Oral Epithelial Cells.","authors":"Yuichiro Kikuchi, Eitoyo Kokubu, Hideo Yonezawa, Kazuyuki Ishihara","doi":"10.2209/tdcpublication.2025-0013","DOIUrl":"10.2209/tdcpublication.2025-0013","url":null,"abstract":"<p><p>Porphyromonas gingivalis, a key periodontal pathogen, employs extra-cytoplasmic function (ECF) sigma factors to adapt to the dynamic oral environment and establish chronic infection. Among these, PGN_1740 (SigH) has been implicated in oxidative stress response and iron acquisition, yet its transcriptional regulatory mechanisms remain poorly understood. The aim of this study was to elucidate the functional role of SigH using sigH-deficient mutants. Quantitative reverse transcription-polymerase chain reaction was performed to assess the transcriptional profiles of sigH and its putative target genes, sod and ltp1, throughout the growth of P. gingivalis. Electrophoretic mobility shift assay (EMSA) was conducted to evaluate the direct binding of recombinant SigH to the sod and ltp1 promoter regions. Oxygen sensitivity was also assessed using hydrogen peroxide susceptibility and cell invasion assays. The results of these experiments revealed that sigH transcription peaked during the mid-log and early stationary phases, followed by a significant decline in the late stationary phase. The EMSA analysis demonstrated that rSigH directly binds to the ltp1 promoter, but not to the sod promoter. Notably, the sigH-deficient mutant exhibited increased oxidative stress sensitivity in the hydrogen peroxide susceptibility assay. Furthermore, unlike the wild type, the sigH-deficient mutant was unable to invade telomerase-immortalized gingival keratinocytes. These findings suggest that the ECF sigma factor SigH regulates oxidative stress tolerance via sod, and iron metabolism via ltp1, in P. gingivalis.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"157-167"},"PeriodicalIF":0.5,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusobacterium nucleatum Increases Growth of Motile Bacterium Treponema denticola without Contact In Vitro.","authors":"Yuichiro Kikuchi, Eitoyo Kokubu, Kazuyuki Ishihara","doi":"10.2209/tdcpublication.2025-0015","DOIUrl":"10.2209/tdcpublication.2025-0015","url":null,"abstract":"<p><p>More than 700 types of bacteria coexist in the human oral cavity, including motile and non-motile taxa. Fusobacterium nucleatum plays an important role in this environment as a bridge between early- and late-colonizing bacteria. We hypothesized that Treponema denticola, a motile bacterium, receives a non-contact benefit from coexisting F. nucleatum. To evaluate this hypothesis, the growth and gene expression of T. denticola were investigated in an environment where F. nucleatum was present but not in contact with it. The results showed that proliferation of T. denticola increased in this environment, and that this was attenuated by autoinducer-2 (AI-2) inhibitor _D-ribose. The results of RNA-seq and qRT-PCR showed that the presence of F. nucleatum increased the gene expression levels of TDE_0039 and TDE_0040. These findings revealed that proliferation of T. denticola increased via a substance produced by F. nucleatum. Importantly, this phenomenon occurs even without direct contact between the two species. In other words, T. denticola may gain a survival advantage by moving through the oral environment, and F. nucleatum may play a crucial role in facilitating this.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"145-156"},"PeriodicalIF":0.5,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tooth Extraction, Dental Visits, and Glucose Levels: a Retrospective Study.","authors":"Seitaro Suzuki, Yoshihito Ohta, Hirozumi Ogawa, Hideyuki Kamijo, Naoki Sugihara","doi":"10.2209/tdcpublication.2025-0005","DOIUrl":"10.2209/tdcpublication.2025-0005","url":null,"abstract":"<p><p>This study explored the association between tooth extraction, dental visit frequency, and blood glucose levels over a 3-year period in middle-aged and older adults using a large health insurance claims database (DeSC, Tokyo, Japan). Data from 31,373 individuals aged 45-70 years who participated in Specified Medical Checkups and Health Guidance between April 2018 and March 2022 were analyzed. Participants were categorized based on the frequency of their annual dental visits and tooth extractions. The primary outcome was high blood glucose level, defined as HbA1c≥6.5%, or fasting plasma glucose level, at ≥126 mg/dl. Individuals who underwent tooth extractions at baseline had lower odds of high blood glucose levels after 3 years, regardless of their baseline glycemic status. Participants with three or more yearly dental visits had lower odds of a high blood glucose level, particularly those with a high baseline blood glucose level and a body mass index ≥25.0 kg/m². These findings suggest an association between regular dental care and improved glycemic control, particularly in individuals with obesity. However, causal relationships cannot be inferred from this study, and further research is required to examine the impact of periodontal treatment and other dental interventions on diabetes management.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"135-143"},"PeriodicalIF":0.5,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal Regenerative Therapy with Recombinant Human Fibroblast Growth Factor-2 and Autogenous Bone Graft in Treatment of Generalized Aggressive Periodontitis (Stage III, Grade C): A Case Report with 1-year Follow-up.","authors":"Rene Harada, Keiko Yamashita, Kentaro Imamura, Satoru Inagaki, Atsushi Saito","doi":"10.2209/tdcpublication.2024-0049","DOIUrl":"10.2209/tdcpublication.2024-0049","url":null,"abstract":"<p><p>This report describes a case of generalized aggressive periodontitis (Stage III, Grade C) requiring periodontal regenerative therapy. The patient was a 35-year-old woman who visited Tokyo Dental College Suidobashi Hospital with the chief complaint of swelling in the maxillary left gingiva. An initial examination revealed 34.5% of sites with a probing depth (PD) of ≥4 mm. The prevalence of sites with bleeding on probing was 29.8%. The plaque control record (PCR) score was 59.8%. Radiographic examination revealed extensive horizontal bone resorption in the maxillary and mandibular molars. Considerable angular bone resorption was observed in teeth #14 and 24. Furcation involvement was observed in teeth #16 and 46. A clinical diagnosis of generalized aggressive periodontitis (Stage III, Grade C) was made, based on which initial periodontal therapy was performed. An improvement was observed in periodontal conditions on re-evaluation. The PCR score was 11.5%. Periodontal surgery was performed for teeth with a residual PD of ≥4 mm. Periodontal regenerative therapy using recombinant human fibroblast growth factor-2 (rhFGF-2) was performed on intrabony defects in #14 and 24. Open flap debridement was performed on #15, 16, 25, and 26. Root separation was performed on #46. Following re-evaluation, an all-ceramic crown was placed on #46, and the patient was placed on supportive periodontal therapy (SPT). During the 1 year of SPT, stable periodontal conditions have been maintained. However, due to the loss of all the secondary molars, the risks of occlusal trauma to the remaining teeth and root surface caries associated with extensive gingival recession following the periodontal treatment remain. Therefore, continued care is necessary to maintain good periodontal conditions.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"123-132"},"PeriodicalIF":0.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solitary Neurofibroma in Lower Lip - A Case Report.","authors":"Masashi Iwamoto, Masato Narita, Ichiro Wakita, Arisa Fujii, Hiroyoshi Yamamoto, Megumi Shingyouchi, Miki Watanabe, Masae Yamamoto, Kazuhiko Hashimoto, Akira Watanabe, Akira Katakura","doi":"10.2209/tdcpublication.2024-0063","DOIUrl":"10.2209/tdcpublication.2024-0063","url":null,"abstract":"<p><p>The WHO Classification of Head and Neck Tumors (2024) defines solitary neurofibromas as benign peripheral nerve sheath tumors comprising a mixture of Schwann cells, perineuronal cells, fibroblasts, and axons. In the orofacial region, such tumors usually occur on the tongue, and only rarely in the lip. This report describes a case of a solitary neurofibroma arising in the lower lip. The patient was a 59-year-old man who was referred to our hospital with a mass on the lower lip. Palpation revealed that it was elastic, hard, painless, and mobile. Magnetic resonance imaging confirmed the presence of a mass. It was identified as a suspected benign tumor and surgical excision conducted under general anesthesia. A diagnosis of solitary neurofibroma was made based on histopathological findings. One and a half years have passed since the surgery, and no recurrence has been noted. Long-term follow-up has been planned.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"117-122"},"PeriodicalIF":0.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Effectiveness of Articulation Practice with Tongue-twister Movement Distance Using Ultrasound.","authors":"Mai Ohkubo, Saki Kuriyama, Haruka Nakata, Tetsuya Sugiyama","doi":"10.2209/tdcpublication.2024-0050","DOIUrl":"10.2209/tdcpublication.2024-0050","url":null,"abstract":"<p><p>Deterioration of oral function can impair articulation, making it essential to evaluate tongue movement during articulation training. Previous reports have indicated an improvement in motor speech disorders, such as glossectomy-related glide issues, through rapid speech exercises, suggesting the potential benefits of non-invasive training. This study aimed to investigate the effect of tongue training on oral function using tongue twisters in healthy Japanese-speaking women. The participants performed tongue twisters designed to engage both the tongue tip and root, with data collected at baseline, 1 month, and 7 months. Participants were required to complete sentences involving the repeated use of /ta/ and /ka/ sounds. This training was conducted 5 times per week for 1 month. Oral diadochokinesis (ODK) was used to measure the number of repetitions per second of each sound, while ultrasound imaging captured the vertical motion of the tongue during pronunciation. The ODK test was used to evaluate speed and regularity of articulatory movement, and ultrasound measurement to assess vertical motion dynamics of the tongue during pronunciation of /ta/ and /ka/. The results indicated a significant improvement in the number of repetitions per second for both /ta/ and /ka/ after 1 month of training. This increase in repetition frequency was maintained for /ta/ even after 7 months, whereas /ka/ showed no further increase in repetition frequency beyond the 1-month mark. Despite this, vertical distance of tongue movement decreased significantly for both syllables after 1 and 7 months. This suggests that /ta/ benefited from both increased efficiency and speed, whereas /ka/ prioritized refined motor control over frequency. The complexity of posterior tongue elevation in /ka/ may require greater precision, limiting increase in repetition despite improved efficiency. This study demonstrates that regular tongue-twister training effectively refines oral function in young women, with sustained benefits over time. The simplicity and accessibility of this method make it a promising approach to maintaining and enhancing oral and articulatory function.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"107-115"},"PeriodicalIF":0.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}