Bulletin of Tokyo Dental College最新文献

{"title":"Activation of Mechanosensitive Ion Channels Induces Autocrine and Paracrine Networks in Craniocervical Squamous Cell Carcinoma Cell via Endothelin Signaling.","authors":"Motoki Ishizaki, Maki Kimura, Sadao Ohyama, Masayuki Ando, Sachie Nomura, Tatsuya Ichinohe, Yoshiyuki Shibukawa","doi":"10.2209/tdcpublication.2024-0018","DOIUrl":"10.2209/tdcpublication.2024-0018","url":null,"abstract":"<p><p>Endothelin (ET) is a peptide comprising 21 amino acids, and its G-protein coupled ETA and ETB receptors are expressed in both cancer cells and cancer-associated cells. Cancer cells excessively express ETA and ETB receptors, and mechanical stimulation promotes the growth and migration of these cells. The autocrine and paracrine signaling of ET is involved in several cancer metabolic pathways, but how this is mediated by the ET-axis (ET and its receptors) remains to be clarified. This study investigated ET-axis-mediated autocrine/paracrine communication in rat squamous cell carcinoma (SCC) in response to direct mechanical stimulation of such cells. Intracellular free Ca²⁺ concentration ([Ca²⁺]_i) was determined using fura-2/AM from a rat SCC cell line (SCC-158). Direct mechanical stimulation of the SCC-158 cells using glass micropipettes to compress the cell membrane to 8 μm for 4 sec induced a transient increase in [Ca²⁺]_i. This increase was also observed in the neighboring cells of the stimulated SCC-158 cells. Treatment with 10 μM Gd³⁺ or 1 μM GsMTx4 almost completely inhibited the mechanical stimulation-induced increase in [Ca²⁺]_i in the SCC-158 cells. Application of 1 μM BQ-123 (an ETA receptor antagonist) inhibited the increase in [Ca²⁺]_i in the stimulated SCC-158 and neighboring cells, whereas that of BQ-788 (an ETB receptor antagonist) had no such effect. These findings suggest that rat SCC cells express the Piezo1 channel. Activation of the Piezo1 channel induced endothelin release from the mechanically stimulated SCC-158 cells. Released ET activated ETA receptors in neighboring SCC-158 cells. These findings also suggest that intercellular paracrine communication among SCC-158 cells through ET signaling plays an important role in the development and metabolism of SCC cells.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"53-63"},"PeriodicalIF":0.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodontic Treatment and 5-year Follow-up in Skeletal Open Bite Case with Congenital Muscular Dystrophy.","authors":"Taiki Morikawa, Teruo Sakamoto, Takenobu Ishii, Yasushi Nishii","doi":"10.2209/tdcpublication.2024-0054","DOIUrl":"10.2209/tdcpublication.2024-0054","url":null,"abstract":"<p><p>Congenital muscular dystrophy (MD) is characterized by progressive muscle weakness. Such patients often present distinctive facial features characterized by excessive vertical growth due to increased muscle breakdown in the perioral muscles such as the masticatory and facial muscles, often resulting in skeletal open bite. Orthognathic treatment is one option for improving the maxillofacial morphology and malocclusion in such patients. However, it is often too risky to apply general anesthesia in such cases due to systemic myofunctional deterioration. Therefore, in many instances, the only option is to rely on orthodontic treatment alone to improve malocclusion. Furthermore, there are few reports on changes following orthodontic treatment in congenital MD patients, and many matters remain unclear regarding long-term occlusal stability. The present case was a girl aged 9 years and 3 months at the initial visit. Her chief complaint was masticatory dysfunction and articulation disorder due to open bite. The first phase of treatment delivered expansion of the upper and lower dental arches and myofunctional therapy prior to transition to the second phase. At the start of the second phase of treatment, the patient was aged 15 years and 9 months. It was determined that orthognathic treatment including surgical invasion would be too risky due general problems related to muscular function. This report describes a case of skeletal open bite due to congenital MD in which an orthodontic approach alone was adopted as camouflage treatment involving extraction of the maxillary left and right second deciduous molars and mandibular left and right first premolars. The post-treatment stability of the resulting occlusion is also described.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"93-104"},"PeriodicalIF":0.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten-year Follow-up of Implant Treatment after Bilateral Lateral Approach to Sinus Elevation with Autologous Bone: A Case Report.","authors":"Yoshitaka Furuya, Tomoki Hirano, Taichi Ito, Hodaka Sasaki","doi":"10.2209/tdcpublication.2024-0007","DOIUrl":"10.2209/tdcpublication.2024-0007","url":null,"abstract":"<p><p>Maxillary sinus floor elevation is an option in cases where bone volume in the maxillary molar region is clearly insufficient to allow implant therapy. However, few studies have reported long-term observation of change in bone volume in the same case over a period of 10 or more years. This report describes a case of implant placement following maxillary sinus floor elevation using iliac bone with long-term monitoring of changes in peri-implant bone volume. The patient was a 58-year-old man who visited our clinic in April 2008 with the chief complaint of chewing problems due to missing teeth. The maxillary sinus floor bone had thinned on both sides, and bilateral maxillary sinus floor elevation using an iliac bone graft was therefore planned. In September 2008, trabecular bone containing bone marrow was harvested from the left side of the ilium, and bilateral maxillary sinus floor elevation was carried out with the patient under general anesthesia. After bone healing for approximately 6 months, a total of eight implants were placed in the maxilla under intravenous sedation in March and April 2009. A telescopic-retained implant-supported fixed bridge was attached as the final superstructure in April 2010. Regarding change in bone volume in the elevated maxillary sinus floor, a reduction in bone height was seen during the period between maxillary sinus floor elevation to implant placement. Following implant placement, however, there was no obvious decrease in bone volume, which has remained stable for 10 years following fitting of the superstructure. Maxillary sinus floor elevation with autologous iliac bone graft has thus shown long-term stability in bone volume over more than 10 years following the procedure. Rigorous follow-up of change in bone volume at the engraftment site is still needed, however.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"65-71"},"PeriodicalIF":0.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant Human Fibroblast Growth Factor (rhFGF)-2 and Autogenous Bone Graft in Periodontal Regenerative Therapy for Stage III Grade C Periodontitis: A Case Report.","authors":"Naoki Miyata, Hideto Aoki, Kentaro Imamura, Atsushi Saito","doi":"10.2209/tdcpublication.2024-0044","DOIUrl":"10.2209/tdcpublication.2024-0044","url":null,"abstract":"<p><p>This report describes a case of periodontitis treated with periodontal surgery incorporating autogenous bone graft and recombinant human fibroblast growth factor (rhFGF) -2. The patient was a 49-year-old woman who visited the Tokyo Dental College Suidobashi Hospital with the chief complaint of swelling in the maxillary right gingiva. Tooth #37 had been extracted 12 years earlier. An initial examination revealed 33.9% of sites with a probing depth (PD) of ≥4 mm. The prevalence of sites with bleeding on probing was 35.2%. The plaque control record (PCR) score was 51.9%. Radiographic examination revealed angular bone resorption in tooth #16. Horizontal adsorption was also observed in other areas. A clinical diagnosis of generalized chronic periodontitis (Stage III Grade C) was made and periodontal therapy initiated. An improvement was observed in periodontal conditions at re-evaluation, with her PCR score decreasing to 15.7%. Periodontal surgery was performed for teeth with a residual PD ≥4 mm. Periodontal regenerative therapy using autogenous bone graft and rhFGF-2 were performed for an intrabony defect in #16. Open flap debridement was performed on #17, 35, and 36. Following evaluation, oral function was restored using a hard resin facing metal crown for #12 and CAD/CAM resin crowns for #14, 15, and 24. The patient was then placed on supportive periodontal therapy. The results showed that periodontal regenerative therapy with autogenous bone graft and rhFGF-2 yielded stable periodontal conditions, which facilitated a favorable level of plaque control.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"83-91"},"PeriodicalIF":0.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal Regenerative Therapy for Stage III Grade B Periodontitis with Degree 2 Furcation Involvement and Angular Bone Defect: A 7-year Follow-up Report.","authors":"Sachiyo Tomita, Takahiro Bizenjima, Ayano Shirahata, Hanako Takayama, Atsushi Saito","doi":"10.2209/tdcpublication.2024-0040","DOIUrl":"10.2209/tdcpublication.2024-0040","url":null,"abstract":"<p><p>This report describes a case of generalized chronic periodontitis requiring periodontal treatment including regenerative therapy. The patient was a 50-year-old woman who visited our clinic requesting periodontal therapy. An initial examination revealed 41.6% of sites with a probing depth (PD) of ≥4 mm and 63.7% of sites with bleeding on probing. The periodontal inflamed surface area (PISA) was 1539.9 mm². Radiographic examination showed furcation involvement in #26, angular bone defect in #46, and horizontal resorption in other regions. Based on a clinical diagnosis of generalized severe chronic periodontitis (Stage III Grade B), initial periodontal therapy consisting of plaque control, and scaling and root planing was performed. Following suppression of inflammation, occlusal adjustment of cuspal interference sites was performed. After re-evaluation, periodontal surgery was performed at selected sites. Periodontal regenerative therapy was performed using autogenous bone graft for #26 and enamel matrix derivative for #46. Other sites with residual periodontal pockets were treated by open flap debridement. Following further re-evaluation, the patient was placed on supportive periodontal therapy. The patient's oral health-related quality of life showed a marked improvement after periodontal therapy. Regenerative therapy resulted in improvement in clinical parameters. Such improvement has been adequately maintained over a 7-year period. The most recent examination revealed 2.4% of sites with a PD of ≥4 mm, and the PISA was 138.2 mm². However, the furcation involvement has not been completely resolved in #26. It is necessary to continue to control inflammation and occlusion and maintain the condition of the periodontal tissue.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"73-82"},"PeriodicalIF":0.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex Odontoma Initially Suspected to be Eruption Sequestrum.","authors":"Hiroki Imai, Seikou Shintani","doi":"10.2209/tdcpublication.2024-0027","DOIUrl":"10.2209/tdcpublication.2024-0027","url":null,"abstract":"<p><p>Various pieces of information, including the age of the patient, the site involved, symptoms, intraoral findings, and X-ray findings are necessary in making a clinical diagnosis. This report describes a case in which the initial diagnosis was eruption sequestrum based on clinical information but which was later found to be a complex odontoma based on histopathological findings. The patient was an 8-year-old girl who presented at our hospital with the chief complaint of pain in the right mandibular molar region. The distal aspect of the right mandibular first molar, which had partially erupted, was covered with gingiva and hard tissue which were separated from the tooth itself. X-ray findings showing radiopaque hard tissue at this site indicated a clinical diagnosis of eruption sequestrum. After local infiltration anesthesia, the hard tissue was extracted and a gingivectomy performed. Histopathological examination of the excised material, however, indicated a diagnosis of complex odontoma.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"25-29"},"PeriodicalIF":0.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal Surgery Using Recombinant Human Fibroblast Growth Factor-2 in Combination with Carbonate Apatite Granules for Stage III Grade C Periodontitis: A 30-month Case Report.","authors":"Yurie Kitamura, Keiko Okuyama, Atsushi Saito","doi":"10.2209/tdcpublication.2024-0029","DOIUrl":"10.2209/tdcpublication.2024-0029","url":null,"abstract":"<p><p>This report describes a case of generalized aggressive periodontitis requiring periodontal treatment including regenerative therapy. The patient was a 34-year-old woman who visited the Tokyo Dental College Suidobashi Hospital with the chief complaint of pain in tooth #14. An initial examination revealed that 35.8% of sites had a probing depth of ≥4 mm, while 19.1% showed bleeding on probing. Radiographic examination revealed angular bone resorption in #14, 33, 36, and 46, with horizontal resorption in other areas. Based on a clinical diagnosis of Stage III Grade C periodontitis, initial periodontal therapy consisting of plaque control, and scaling and root planing was performed. After reevaluation, periodontal regenerative therapy using recombinant human fibroblast growth factor-2 (rhFGF-2) in combination with carbonate apatite (CO₃Ap) granules was performed for #14 and 33, while regenerative therapy using rhFGF-2 alone was performed for #46. Following reevaluation, the patient was placed on supportive periodontal therapy (SPT). During SPT, CAD/CAM crowns were placed on #26 and 46. Periodontal regenerative therapy using rhFGF-2 with CO₃Ap granules yielded a resolution of angular bone defects in #14 and 33. This improvement has been adequately maintained over a 30-month period.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"31-40"},"PeriodicalIF":0.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Porphyromonas Gingivalis Infection on Epithelial Rests of Malassez.","authors":"Eitoyo Kokubu, Yuichiro Kikuchi, Hideo Yonezawa, Hodaka Sasaki, Kenichi Matsuzaka, Kazuyuki Ishihara","doi":"10.2209/tdcpublication.2024-0028","DOIUrl":"10.2209/tdcpublication.2024-0028","url":null,"abstract":"<p><p>The epithelial cell rests of Malassez (ERM) are located within the periodontal ligament. They are reportedly involved in maintaining homeostasis, particularly with regards to the thickness of the periodontal ligament. Their role in apical periodontitis lesions remains unclear, however. This study investigated the response of ERM to infection with Porphyromonas gingivalis. After being infected, the morphology of the P. gingivalis-infected cells was observed using confocal laser-scanning microscopy. The gene expression of P. gingivalis-infected and uninfected cells was investigated by RNA-sequencing analysis. Morphological observation showed the invasion and adhesion of P. gingivalis to the surface of ERM. The RNA analysis showed that the gene expression profile significantly differed between the infected and uninfected cells. At an expression level of ≥2 and false discovery rate of <0.1, the infected cells showed a decrease in 99 genes and an increase in 6 compared with in the non-infected cells. Most of the upregulated genes were unique to epithelial cells, such as endothelial cell-specific molecules and cytokeratin 5; the upregulated genes were associated with the immune response, however. These results indicate that ERM upregulate genes associated with epithelial cells and suppress those associated with the immune response following P. gingivalis infection.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"13-23"},"PeriodicalIF":0.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Orthodontic Study of Non-syndromic Oligodontia: An Examination of Occlusion and Occlusal Support.","authors":"Yasuko Tazuhama, Haruyo Miyazaki, Mihoko Hirai, Yuka Hoshino, Takenobu Ishii, Yasushi Nishii","doi":"10.2209/tdcpublication.2024-0017","DOIUrl":"10.2209/tdcpublication.2024-0017","url":null,"abstract":"<p><p>The aim of this study was to analyze orthodontic data to investigate occlusal conditions, the relationship between malocclusion and the number of congenitally missing teeth, and occlusal support of maxillomandibular teeth in patients with oligodontia. The study included 66 patients with permanent dentition from two orthodontic clinics belonging to Tokyo Dental College who had received a diagnosis of oligodontia between 2003 and 2014. The materials used for the analysis comprised intraoral photographs, panoramic radiographs, and lateral cephalometric radiographs. The antero-posterior occlusal relationships, assessed by overjet, showed a high prevalence of anterior crossbite, while the vertical occlusal relationships, evaluated by overbite, indicated a predominance of deep bite. There were no significant differences in the total number of missing teeth in the three groups divided according to degree of overjet. The same tendency was observed in the case of overbite. The number of missing teeth in the maxilla was significantly higher than that in the mandible. The high occurrence of anterior crossbite in oligodontia is believed to be related to skeletal factors, such as deficiency of the maxillary apical base and a greater number of missing teeth in the maxilla compared to in the mandible. Based on their Eichner Index scores, 98.5% of cases were classified as Class B. Among these, B2 was the most prevalent (60.6%), suggesting that patients with oligodontia have low occlusal support. The average number of Occlusal Units (OUs) was 6.4 out of 12. Nearly half of the second deciduous molars were retained in both the maxilla and the mandible. When deciduous molars were treated as premolars, there was a significant increase in number of OUs. No significant differences were observed in the actual occlusal support score, however. Therefore, oligodontia with many defects in the premolar region resulted in a low level of occlusal support, and although the survival rate of the deciduous molars was high, they were not useful in providing occlusal support.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"1-11"},"PeriodicalIF":0.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treponema Denticola Activates NF-κB Pathway via Toll-like Receptor 2.","authors":"Eitoyo Kokubu, Yutaro Ando, Yuichiro Kikuchi, Hideo Yonezawa, Kazuyuki Ishihara","doi":"10.2209/tdcpublication.2024-0023","DOIUrl":"10.2209/tdcpublication.2024-0023","url":null,"abstract":"<p><p>Treponema denticola is frequently isolated together with Porphyromonas gingivalis from the lesions seen in cases of chronic periodontitis and is considered a major pathogen of this disease. It has several virulence factors, including a major surface protein (Msp) and a major surface protease, dentilisin. The effect of these virulence factors on the host immune response remains to be elucidated, however. Toll-like receptors (TLRs) in the host can recognize pathogen-associated molecular patterns. Bacteria stimulate TLRs and activate the pro-inflammatory nuclear factor-kappa B pathway. Therefore, the aim of this study was to investigate the effect of T. denticola on TLR pathways. Toll-like receptor 4 and TLR2 reporter cell lines, which secrete alkaline phosphatase in response to TLR signals, were infected with the T. denticola wild type, an Msp-deficient mutant, a dentilisin-deficient mutant, or their extracts obtained via sonication. Signals from TLR2 or TLR4 cells were evaluated by alkaline phosphatase activity. Toll-like receptor 2 signals were detected in all T. denticola strains and sonication extracts, while no TLR4 signal was detected. Infection with the dentilisin-deficient mutant induced the strongest TLR2 signal among the strains. Sonication extracts of the wild type and Msp-deficient mutant showed the same level of TLR2 signaling. The TLR2 signal in the sonication extracts from the wild type was inhibited by Sparstolonin B, an antagonist of TLR2, in a dose-dependent manner. These results indicate that T. denticola is recognized by epithelial cells mainly via TLR2. The outer sheath structure may conceal potential ligands for TLR2.</p>","PeriodicalId":45490,"journal":{"name":"Bulletin of Tokyo Dental College","volume":" ","pages":"41-50"},"PeriodicalIF":0.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}