2010 IEEE International Conference on Nano/Molecular Medicine and Engineering最新文献_第4页

Hysteresis modeling and tracking control of a piezostage for biological cell manipulation 生物细胞操纵压电平台的迟滞建模与跟踪控制

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749803

Qingsong Xu, Yangmin Li

引用次数: 0

Nondestructive imaging of intracellular serotonin in intact JAR cells using antibody conjugated quantum dot 抗体偶联量子点无损成像完整JAR细胞内血清素

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749840

Jiyeon Kim, Chaeyun Lim, J. Song

{"title":"Nondestructive imaging of intracellular serotonin in intact JAR cells using antibody conjugated quantum dot","authors":"Jiyeon Kim, Chaeyun Lim, J. Song","doi":"10.1109/NANOMED.2010.5749840","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749840","url":null,"abstract":"Direct measurement of neurotransmitter in a single cell still remains a major challenge. Existing probes have several problems for accurate observation of intracellular component. In recent years, quantum dots (Qdot), with their unique physical, chemical, and optical properties, have been used extensively as probes to visualize several cell membrane receptors and extracellular biomolecules. However, high affinity between quantum dots may induce serious aggregation in the cytoplasm; as a result, quantum dot aggregates are usually misinterpreted as quantum dot-probed intracellular molecules. Moreover, higher viscosity of cytoplasm compared to the extracellular aqueous media may lead to a higher number of aggregated quantum dots. To eliminate these obstacles, we developed a direct nondestructive serotonin imaging in an intact cell using the quantum dot-based immunoassay with a rapid tunable multicolor imaging system based on the acousto-optic tunable filter. It provides rapid image scanning of serotonin granules as a function of wavelength under conditions in which the serotonin granules are bound with different colored quantum dots anti-serotonin antibody conjugates. This multicolor imaging technique clearly does away with aggregation issues that emerge from the use of quantum dots as probes for intracellular molecules. The results of this work demonstrate that multicolor imaging combined with fluorescent semiconductor nanocrystals has the potential to serve as a viable technique for nondestructive intracellular serotonin measurements in intact cells.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115319770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic particle sorter based on optical tweezers array system 基于光镊阵列系统的微流控颗粒分选

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749798

Qin Li, Jingfang Li, Xiaoming Hu

引用次数: 1

Insulin detection based on a PDMS microfluidic system 基于PDMS微流体系统的胰岛素检测

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749815

Zhikun Zhan, Ping Yao, Z. Dong, S. Tung, J. Hohnbaum, B. Srinivasan, W. J. Li

引用次数: 6

Enzymatic glucose biosensor based on porous ZnO/Au electrodes 基于ZnO/Au多孔电极的酶促葡萄糖生物传感器

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749805

Xueqiu You, Jungil Park, Y. Jang, Soo-won Kim, J. Pak, N. Min

引用次数: 5

Fabrication of SERS nanoprobe and its application to cancer cell imaging SERS纳米探针的制备及其在癌细胞成像中的应用

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749814

Sangyeop Lee, J. Choo

引用次数: 2

Real-time bioimaging of hyaluronic acid derivatives using quantum dots for biopharmaceutical delivery applications 利用量子点对透明质酸衍生物进行实时生物成像，用于生物制药输送应用

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749827

K. Kim, Jiseok Kim, Kitae E. Park, Min-young Lee, Jeonga Yang, Eun Ju Oh, S. Hahn

{"title":"Real-time bioimaging of hyaluronic acid derivatives using quantum dots for biopharmaceutical delivery applications","authors":"K. Kim, Jiseok Kim, Kitae E. Park, Min-young Lee, Jeonga Yang, Eun Ju Oh, S. Hahn","doi":"10.1109/NANOMED.2010.5749827","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749827","url":null,"abstract":"Hyaluronic acid (HA), which has been used for various medical applications, is a biocompatible, biodegradable, and linear polysaccharide in the body. In this work, real-time bioimaging of HA derivatives was carried out using quantum dots (QDot) for target specific and long acting delivery applications of protein, peptide, and nucleotide therapeutics. In vitro bioimaging of HA-QDot conjugates successfully visualized the target specific delivery of HA derivatives to B16F1 cells with HA receptors by HA receptor mediated endocytosis, which reflects the feasibility of HA derivatives for intracellular delivery of biopharmaceuticals, especially nucleotide therapeutics. Furthermore, according to in vivo real-time bioimaging, HA-QDot conjugates with 35 mol% HA modification were mainly accumulated in the liver, while those with 68 mol% HA modification were evenly distributed to the tissues in the mice. Accordingly, slightly modified HA derivatives were used for target-specific delivery of biopharmaceuticals for the treatment of liver diseases and highly modified HA derivatives were used for long acting conjugation of peptide and protein therapeutics. These HA-based biopharmaceutical delivery systems can be exploited for further clinical development.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133718141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Vertically aligned carbon nanofiber neural chip for interfacing with neurological system 垂直排列的碳纳米纤维神经芯片与神经系统的接口

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749832

Zhe Yu, T. McKnight, M. Ericson, A. Melechko, M. Simpson, B. Morrison

引用次数: 3

Luminescent iridium(III) polypyridine PEG complexes: Synthesis, photophysical, and biological properties 发光铱(III)聚吡啶聚乙二醇配合物:合成、光物理和生物性质

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749807

S. Li, Johnson Lui-Lui Tsai, K. K. Lo

{"title":"Luminescent iridium(III) polypyridine PEG complexes: Synthesis, photophysical, and biological properties","authors":"S. Li, Johnson Lui-Lui Tsai, K. K. Lo","doi":"10.1109/NANOMED.2010.5749807","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749807","url":null,"abstract":"We report the synthesis, characterization, and photophysical properties of a class of luminescent cyclometalated iridium(III) polypyridine poly(ethylene glycol) (PEG) complexes [Ir(N⁁C)<inf>2</inf>(bpy-CONH-PEG<inf>n</inf>)](PF<inf>6</inf>) (HN⁁C = Hppy (1a), HN⁁C = Hpq (2a), HN⁁C = Hpba (3a)). The discrete PEG complexes [Ir(N⁁C)<inf>2</inf>(bpy-CONH-dPEG<inf>24</inf>)](PF<inf>6</inf>) (HN⁁C = Hppy (1b), HN⁁C = Hpq (2b), HN⁁C = Hpba (3b)) and the PEG-free complexes [Ir(N⁁C)<inf>2</inf>(bpy-CONH-Et)](PF<inf>6</inf>) (HN⁁C = Hppy (1c), HN⁁C = Hpq (2c), HN⁁C = Hpba (3c)) have also been prepared for comparison studies. The lipophilicity of the complexes has been determined by shake-flask method. We have also investigated the cytotoxicity and cellular uptake of these complexes by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay and laser-scanning confocal microscopy, respectively. The results illustrated that the nondiscrete PEG complexes can act as biological imaging reagents with low cytotoxicity. Co-localization experiments using an endocytic marker Alexa Fluor 633-labeled transferrin revealed the involvement of endosomes in the uptake of the complexes by the human cervix epithelioid carcinoma (HeLa) cells. Since the aldehyde groups of the pba complexes 3a-c are reactive toward primary amines, they have been conjugated to bovine serum albumin (BSA) and the resulting conjugates have been isolated, purified, and its photophysical properties studied. Complexes 3a and 3b serve as new luminescent PEGylation reagents.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128261852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a catheter operating system for medical applications 医用导管操作系统的研制

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749795

Nan Xiao, Shuxiang Guo, Jian Guo

引用次数: 2