2010 IEEE International Conference on Nano/Molecular Medicine and Engineering最新文献_第5页

High-throughput screening of xanthine oxidase inhibitory properties of drug analogs using photodiode array microchip 利用光电二极管阵列芯片高通量筛选药物类似物黄嘌呤氧化酶抑制特性

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749839

Jae Hyung Lee, Jae Ah Kim, J. Song

{"title":"High-throughput screening of xanthine oxidase inhibitory properties of drug analogs using photodiode array microchip","authors":"Jae Hyung Lee, Jae Ah Kim, J. Song","doi":"10.1109/NANOMED.2010.5749839","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749839","url":null,"abstract":"We have developed a high-throughput chip-based assay that uses a photodiode array (PDA) microchip system to explore the inhibitory effects of drug analogs on Xanthine oxidase (XO). Inhibitory effects of dithranol (anthracene derivative), aminoglutethimide (anti-steroid), cyclosporine A (immunosuppressant) and naringenin (flavonoid) on XO were elucidated using the chip-based assay in the presence or absence of a free radical scavenging enzyme (SOD, superoxide dismutase). Drug analogs were assessed for their ability to inhibit XO, which loads to a reduction in the conversion of nitroblue tetrazolium (NBT) to NBT diformazan. The reduction of NBT to NBT formazan in the presence of XO and drug analog can be seen. The PDA microchip system employed in this study consists of an array of red light-emitting diodes (LEDs) focused precisely onto the photodetectors of the PDA chip. The ability of drugs to inhibit XO was assessed based on NBT reduction. Free radicals produced due to the oxidation of xanthine by XO resulted in the reduction of NBT to insoluble NBT formazan. We report a new high-throughput chip-based xanthine assay to explore the XO inhibitory properties of drug analogs, along with their modes of action. The work presented here has important implications with regard to rapid and automated drug discovery screening processes in the pharmaceutical industry.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122994467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing the micromechanical properties of myeloblasts from cancer patients with optical tweezers 用光学镊子表征癌症患者骨髓母细胞的微力学特性

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749800

Youhua Tan, A. Y. Leung, Kaiqun Wang, T. Fung, Dong Sun

{"title":"Characterizing the micromechanical properties of myeloblasts from cancer patients with optical tweezers","authors":"Youhua Tan, A. Y. Leung, Kaiqun Wang, T. Fung, Dong Sun","doi":"10.1109/NANOMED.2010.5749800","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749800","url":null,"abstract":"Cell mechanics, in particular mechanical properties, has been suggested as a new biomarker indicative of cell state and phenotype. Acute myeloid leukemia (AML) is characterized by the abnormal increase of myeloblasts in blood and bone marrow. While AML has been extensively studied from the perspectives of biochemical and genetic aspects, little is known about its cellular biophysical properties. In this study, optical tweezer technology was used to examine the micromechanical properties of myeloblasts from bone marrow of AML patients at single cell level. The myeloblasts were separately analyzed according to their expression of CD34+, a marker of primitive hematopoietic cells. To extract the intrinsic properties from the relationship between the stretching force and the induced deformation, a theoretical approach was developed to model the mechanical responses of cells and further characterize their mechanical properties. The preliminary results show that the area compressibility modulus of CD34+ myeloblasts was significantly less than that of CD34− cells, which indicate that micromechanical properties are unique features of myeloblasts and provide us with an insight into the cell mechanics of primitive AML cells.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122614381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multicolor single cell imaging cytometry: A new drug screening platform for monitoring intracellular caspases as potential therapeutic targets 多色单细胞成像细胞术:监测细胞内半胱天冬酶作为潜在治疗靶点的新药物筛选平台

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749843

Min Jung Kim, J. Song

{"title":"Multicolor single cell imaging cytometry: A new drug screening platform for monitoring intracellular caspases as potential therapeutic targets","authors":"Min Jung Kim, J. Song","doi":"10.1109/NANOMED.2010.5749843","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749843","url":null,"abstract":"SH-03 is a rotenoid-containing deguelin analog. In this study, the anticancer activity and the dynamics of the caspase-mediated apoptotic cascade induced by SH-03 was simultaneously monitored in myeloid leukemia cells using multicolor single cell imaging cytometry as a new drug-screening platform. The constructed single cell imaging cytometer consists of an Ar-ion laser beam, a microscope objective lens, an acousto-optic tunable filter (AOTF), a charged coupled device (CCD) camera, and commercially available image processing software (MetaMorph). PI3K/AKT activation and phosphorylation assays were carried out to clarify the mechanistic details of the SH-03-induced intrinsic apoptotic pathway in HL-60 cells. SH-03 inhibited the PI3K/AKT signal transduction pathway in HL-60 cells. Given the fact that PI3K/AKT signaling pathway plays an important role in the proliferation, the inhibition of phosphorylation of PI3K/AKT by SH-03 can be assumed to trigger apoptosis. SH-03-induced caspase cascade was also investigated. Our results suggest that SH-03 induces apoptosis in HL-60 cells via a caspase-9-mediated intrinsic apoptotic pathway. Cellular imaging cytometry revealed that SH-03 triggers apoptosis via a caspase-mediated intrinsic pathway.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"68 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129329754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depth of Anaesthesia: Measuring or guessing? 麻醉深度:测量还是猜测?

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749809

Richard Landers, P. Wen, S. Pather

引用次数: 5

Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes 发光多吡啶芴配合物作为新型生物探针

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749806

Man-Wai Louie, Tommy Tsz-Him Fong, K. K. Lo

引用次数: 2

Laminar stream-based microfluidic chip with high efficiency for human sperm motility sorting 基于层流的高效人类精子活力分选微流控芯片

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749808

Hui-Ting Fu, D. Yao, Hung-Ju Huang, Chin-Jung Li, Hong-Yuan Huang

{"title":"Laminar stream-based microfluidic chip with high efficiency for human sperm motility sorting","authors":"Hui-Ting Fu, D. Yao, Hung-Ju Huang, Chin-Jung Li, Hong-Yuan Huang","doi":"10.1109/NANOMED.2010.5749808","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749808","url":null,"abstract":"A novel laminar stream-based microfluidic chip that can serve as the high efficient technique for human sperm motility sorting through the use of flow cytometry was proposed in this study. Recently, the selection of good sperms in semen plays an important role in the reproductive medicine. However, the traditional sorting methods are normally expensive and time consuming. This modified microfluidic chip provided a new selection, which had advantages of low-cost, high-precision, and disposable. This paper was proposed and tested the interactions between laminar flow and sperm activity. Due to the laminar flow in a different direction, the semen samples were loaded into a curve laminar flow through straight-curve stream and it would offer a y component force for dead sperms to be selected. In addition, the semen samples were loaded into a straight laminar flow and the results showed that most of the proportion of motile sperms with lateral activity can swim across the two fluid boundaries to be sorted. From the results of flow cytometry analysis, the injected semen sample from a straight inlet with the counted percentage of living sperms was 92.68% by the observation in outlet reservoir. Therefore, the microfluidic chip with remarkable sperm motility sorting has the potential for reproductive medicine.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128240957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Observation of angiotensin II-induced changes in tubular epithelial cells utilizing AFM: Angiotensin II-induced changes in tubular cells 利用AFM观察血管紧张素ii诱导的小管上皮细胞的变化:血管紧张素ii诱导的小管细胞的变化

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749834

Gi-Ja Lee, Ji-Hye Kim, Hun-Kuk Park, K. Jeong, Hyun-jung Kang, T. Lee

引用次数: 1

Silver as antibacterial agent: Metal nanoparticles to nanometallopharmaceuticals: (Silver based antibacterial nanometallopharmaceuticals) 银作为抗菌剂:金属纳米颗粒到纳米金属药物:(银基抗菌纳米金属药物)

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749812

J. Song

{"title":"Silver as antibacterial agent: Metal nanoparticles to nanometallopharmaceuticals: (Silver based antibacterial nanometallopharmaceuticals)","authors":"J. Song","doi":"10.1109/NANOMED.2010.5749812","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749812","url":null,"abstract":"Because of increase in bacterial resistance to common antibiotics, antibacterial nanometallopharmaceuticals have been drawing increasing interest. Recent literature reports encouraging results about the bactericidal activity of silver nanoparticles of either a simple or composite nature. We, for the first time, demonstrated that silver nanoparticles (AgNP) undergo a shape dependant interaction with bacteria. We also developed highly antibacterial porous carbon matrices supporting nano-silver by simple and cost effective way. The antibacterial mechanism of the developed composite under prolonged incubation conditions was investigated against Escherichia coli. The application of silver in the treatment of burn wounds is of special interest, and has prompted an upsurge in research on the synthesis of antibacterial silver(I) complexes. However, the antibacterial performance of silver ion is correlated to its valence form and it has been found that the high valence silver ion exhibits stronger and more effective antibacterial action than low valence ion. Recently, we reported the synthesis of highly monodispersed nanoparticles of a trivalent silver polydiguanide complex in reverse microemulsion, demonstrating its flexibility for complexation reaction and the fabrication of essentially monodispersed nanoparticles of higher valent metal complex. The synthesized material showed strong antibacterial activity against the tested Gram (+)/(−) and methicillin-resistant Stahylococcus aureus strains. Interaction of polydiguanide ligands with silver at different oxidation states was also investigated. The in vitro pharmacodynamics of these complexes revealed that silver polydiguanide complexes provide faster killing kinetics compared to silver nitrate or polydiguanide ligands.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122000556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy 用原子力显微镜测定核酸双链间结合亲和力

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749818

Tianbiao Zhang, Changlin Zhang, Zai-li Dong, Y. Guan

{"title":"Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy","authors":"Tianbiao Zhang, Changlin Zhang, Zai-li Dong, Y. Guan","doi":"10.1109/NANOMED.2010.5749818","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749818","url":null,"abstract":"Atomic force microscope (AFM), as its high resolution and precision, was employed to directly probe intermolecular hydrogen bonds between self-assembled purines and pyrimidines. In the experiment, we directly stretch the DNA double strands that are attached to the planar modified glass surfaces and AFM tip respectively. However, distinguishing from previous literatures we focus on the NaCl density and holding time influencing the rupture force of DNA double helix and monitor the probing process under three different densities of sodium chloride (NaCl): 0mM, 50mM, and 100mM. The result shows complementary DNA strands are easier to bond as the density of NaCl increases. At the same time we test the probing process considering three holding durations: 0S, 10S, and 40S. We find the complementary DNA strands bond forming well under the situation of 40S. Then we separately do experiments with three lengths of strands: 10bp, 14bp, 20bp. Using cluster analysis method we find that the clustering center intervals are about 0.21nN, 0.29nN, and 0.39nN, and we can calculate the hydrogen bond of the single G-C is about 20pN.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126954074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Low-cost portable respiration monitor based on micro hot-film flow sensor 基于微热膜流量传感器的低成本便携式呼吸监测仪

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.6107964

Zhe Cao, R. Zhu, R. Que

引用次数: 3