2010 IEEE International Conference on Nano/Molecular Medicine and Engineering最新文献

The design and investigation of model based internal model control for the regulation of hypnosis 基于模型的催眠调节内模控制的设计与研究

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-05 DOI: 10.1109/NANOMED.2010.5749833

S. Abdulla, P. Wen, W. Xiang

引用次数: 5

Development and experiments of a novel multifunctional underwater microrobot 一种新型多功能水下微型机器人的研制与实验

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749794

Liwei Shi, Shuxiang Guo, K. Asaka, Shilian Mao

{"title":"Development and experiments of a novel multifunctional underwater microrobot","authors":"Liwei Shi, Shuxiang Guo, K. Asaka, Shilian Mao","doi":"10.1109/NANOMED.2010.5749794","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749794","url":null,"abstract":"Compact structure, multifunction, and flexibility are normally considered as incompatible characteristics for legged microrobots. Most robots focused on complex structure of multi-joint legs to attain the multifunction and flexibility, while others had poor flexibility for miniaturization. In the field of underwater monitoring for applications such as pollution detection and video mapping in limited space, underwater microrobots are urgently demanded. To realize these purposes, we have developed several types of microrobots with both compact structure and flexible locomotion. However, they just realized walking, rotating, swimming, or floating motions. Without biomimetic fingers, they could not do some simple operations, such as grasping and carrying any objects to desired place. So, in this paper, we designed a novel type of biomimetic locomotion employing ionic polymer metal composite (IPMC) actuator as one-DOF leg. Then we proposed a new type of underwater microrobot using ten ionic polymer metal composite (IPMC) actuators as legs or fingers, which could realize walking, rotating, floating, and grasping motions. Also, we developed a prototype of this underwater microrobot and carried out some experiments to evaluate its walking and floating speeds. In addition, we used six IPMC actuators as fingers to grasp some small objects and float up. To realize the closed-loop control for the microrobot, we used three proximity sensors to detect and avoid the obstacle while walking.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117204983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Investigations of Bio Markers for ion channel activities on insulinoma cells 胰岛素瘤细胞离子通道活性生物标志物的研究

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749830

Ruiguo Yang, N. Xi, K. Lai, C. Fung, Chengeng Qu, Beihua Zhong, Donna H. Wang

{"title":"Investigations of Bio Markers for ion channel activities on insulinoma cells","authors":"Ruiguo Yang, N. Xi, K. Lai, C. Fung, Chengeng Qu, Beihua Zhong, Donna H. Wang","doi":"10.1109/NANOMED.2010.5749830","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749830","url":null,"abstract":"Ion channel is the regulatory mechanism for electrical activity in pancreatic islet cells through stimulus-secretion coupling. Changes in membrane potential are regulated by the glucose concentration-dependent ion channel activities. The alteration of glucose concentration is linked to the open probability of ATP-sensitive K+ channels by insulin secretion. At the meantime, the change of glucose concentration can cause the reorganization of the membrane as well as the cytoskeleton, resulting in the change of cellular stiffness. By using an integrated AFM and cell manipulation system, we were able to measure the cell stiffness and structural change simultaneously upon the glucose stimulation. The cell stiffness increases substantially in a dosage-dependent manner after stimulation by real time AFM nanoindentation measurement. Structurally, the cell height decrease dynamically with the glucose concentration increase. Therefore we have a unique Bio Marker to characterize the ion channel activity using different modalities. This result indicates that the open and close of ion channel would lead to the change of membrane structure and thus the cell body exhibits a different cellular stiffness. The study will enhance our understanding of pancreatic islet cell stimulus coupling and insulin secretion.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130515845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Measuring the molecular force of Burkitt's lymphoma patient cells using AFM 利用原子力显微镜测量伯基特淋巴瘤患者细胞的分子力

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749829

Mi Li, Lianqing Liu, N. Xi, Yuechao Wang, Z. Dong, Guangyong Li, Xiubin Xiao, Weijing Zhang

引用次数: 0

Real time identification of apoptosis signaling pathways using AFM-based nano robot 利用基于afm的纳米机器人实时识别细胞凋亡信号通路

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749816

Ruiguo Yang, C. Fung, K. Seiffert-Sinha, N. Xi, K. Lai, A. Sinha

{"title":"Real time identification of apoptosis signaling pathways using AFM-based nano robot","authors":"Ruiguo Yang, C. Fung, K. Seiffert-Sinha, N. Xi, K. Lai, A. Sinha","doi":"10.1109/NANOMED.2010.5749816","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749816","url":null,"abstract":"Apoptosis is the process of programmed cell death that occurs in multi-cellular organisms. The apoptosis process of animal cells is characterized by a series of changes on the cell body such as loss of membrane symmetry, cell shrinkage, chromatin condensation and DNA fragmentation. Apoptosis is of great importance as a cellular process, whereas the malfunction of which would lead to a variety of disease including cancer. In a potential fatal skin disease pemphigus vulgaris (PV), it is found that auto-immune antibody will induce the apoptosis of keratinocytes, the main epithelial cell that forms the skin, resulting in the blistering of the skin. The exact mechanism of apoptosis in PV is not well understood. Atomic force microscopy (AFM), originally invented as a high resolution imaging tool, recently finds wide applications in biological science with its unique properties. It can operate in liquid where physiological conditions for biological matters can be kept, which makes it an ideal tool for structural characterization of live cells. Besides, it is a natural nanoindenter with high sensitivity and can both measure and apply extremely small forces. Furthermore, the AFM-based nanomanipulation system makes it even more convenient to change things in the molecular level. By applying the AFM-based nanorobotic system to the cellular model system in PV via statistical analysis of nanoindentation data obtained in real time combined with high resolution structural characterization, we find that the cells become stiffer when apoptosis early process begin. This methodology and technique will facilitate the diagnosis and treatment of PV in the future.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130741985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Noncontact and contact micromanipulation using a rotating nickel nanowire 使用旋转镍纳米线的非接触和接触微操作

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749802

Li Zhang, T. Petit, K. Peyer, B. Kratochvil, Jiangnan Zhang, J. Lou, B. Nelson

引用次数: 4

Measuring Primary Hepatocyte adhesion on polyelectrolyte multilayer films by a passive detachment sensing tool 用被动分离传感工具测量原代肝细胞在聚电解质多层膜上的粘附

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749817

Yantao Shen, Zheng Chen, Xiaobo Tan

引用次数: 0

3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs 三维基质粘连介导hmsc -胶原结构的机械传导

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749801

Terry Li, B. Chan

{"title":"3D matrix adhesions mediating mechanostranduction in hMSC-collagen constructs","authors":"Terry Li, B. Chan","doi":"10.1109/NANOMED.2010.5749801","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749801","url":null,"abstract":"The current study aims to identify the type of cell-matrix adhesions of hMSCs in 3D collagen constructs and to investigate the effects of dynamic compression on the type, morphology and composition of cell-matrix adhesions, particularly to observe whether the compression stimulates the maturation or evolvement of 3D matrix adhesion in hMSC-collagen constructs. Preliminary results demonstrated the colocalization of integrin α5 β1 and fibronectin in cell-matrix adhesions in loaded constructs, partially fulfilling the requirements for 3D matrix adhesion to evolve. In addition, fibronectin was shown to be organized into tiny-dotted adhesions in loaded constructs in a loading duration dependent way, suggesting dynamic compression may be able to mature adhesions in the constructs, hopefully into 3D matrix adhesions. It was also demonstrated that hMSCs plated onto their own cell-derived matrices form elongated adhesions which are similar to 3D matrix adhesions formed by fibroblasts. Further characterization on the cell-matrix adhesions of hMSCs in 3D collagen constructs and identification of differences in adhesions between loaded and unloaded constructs are underway.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"178 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114261989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-penetration efficiency of PEGylated multi-walled carbon nanotubes is dependent on cell types 聚乙二醇化多壁碳纳米管的细胞穿透效率与细胞类型有关

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749835

Jinping Cheng, Y. Lam, Ya‐Ping Sun, S. Cheng

{"title":"Cell-penetration efficiency of PEGylated multi-walled carbon nanotubes is dependent on cell types","authors":"Jinping Cheng, Y. Lam, Ya‐Ping Sun, S. Cheng","doi":"10.1109/NANOMED.2010.5749835","DOIUrl":"https://doi.org/10.1109/NANOMED.2010.5749835","url":null,"abstract":"Carbon nanotubes (CNTs) have been widely investigated as one of the most promising nanomaterials in biomedical applications. Functionalization of CNTs with poly(ethylene glycol) diamine (PEG) is a recognized methodology with good solubility and biocompatibility. In this study, PEG conjugated (PEGylated) multi-walled CNTs (MWCNTs) were prepared and labeled with fluorophore FITC. Using fluorophore-conjugated PEGylated MWCNTs, we monitored their accumulation in transformed cancer cells and in normal cells. The intracellular accumulation of PEGylated MWCNTs was studied under confocal microscope and transmission electron microscope. At the same concentration and exposure time, PEGylated MWCNTs entered all three of the cancer cell lines tested much more efficiently than the three primary human fibroblast lines. This different cell-penetration efficiency was observed both in separate cell culture separate exposure conditions and in co-culture and co-exposure conditions. This study highlights that the cell-penetration efficiency of PEGylated MWCNTs is dependent on cell types, and possibly due to different metabolic rates in different cell types. Furthermore, the intracellular accumulation of PEGylated MWCNTs did not impair membrane integrity, and the treated cells remained normal morphology, indicating good biocompatibility of PEGylated MWCNTs. This study suggests that PEGylated MWCNTs can be developed as potential drug carrier with its intrinsic higher preference to tumor cells than normal cells.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133724263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autofocusing algorithm comparison in bright field microscopy for automatic vision aided cell micromanipulation 自动视觉辅助细胞显微操作的明场显微镜自动聚焦算法比较

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2010-12-01 DOI: 10.1109/NANOMED.2010.5749811

M. Y. Yu, M. Han, C. Shee, W. T. Ang

引用次数: 9