Measuring Primary Hepatocyte adhesion on polyelectrolyte multilayer films by a passive detachment sensing tool

Abstract

This paper presents quantitative measurements of Primary Hepatocyte (rat liver cell) adhesion on polyelectrolyte multilayer (PEM) films. Measurements are implemented through detaching the cell from the film using a developed highly sensitive micro-force sensing tool. Experimental results demonstrate the measured detaching or adhesion forces of cells on polyelectrolyte multilayer films with different number of bilayers during detachment. These results provide an essential step toward better understanding of the cell-material interaction, and could contribute to more significant research in the fields of tissue engineering and liver disease.