Water Research最新文献

{"title":"Self-Recycled Electron Donor Resists Disfavored Oxidation Reconstruction of Cu(I)-based Electrocatalyst for Nitrate Removal by Charge Compensation","authors":"Jiaqi Chen, Yuan Yao, Yu Yan, Xiaoxiao Li, Yang Liu","doi":"10.1016/j.watres.2024.122959","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122959","url":null,"abstract":"The overuse of nitrate has led to the accumulation in natural water, being a globe issue in environment and human health. Electrochemical NO₃⁻ reduction reaction (eNO₃RR) to ammonia occurs under ambient condition with low energy consumption and the yield of value-added product, being promising for NO₃⁻ removal. Cu^(I)-based eNO₃RR catalysts suffer from unavoidable oxidation reconstruction to Cu^(II), reducing the performance of NO₃⁻ removal. In this work, we demonstrate charge compensation strategy to resist oxidation reconstruction of Cu^(I)-based eNO₃RR catalysts by introducing self-recycled electron donor. Taking Ti^(III)-modified Cu₂O/Cu as the proof-of-concept model, electron donor Ti^(III) can donate electron to Cu^(II) to regenerate Cu^(I), meanwhile the expended Ti^(III) can be recycled from the generated Ti^(IV) via intervalence charge transfer (IVCT). Benefiting from those, Ti-Cu₂O/Cu-10 exhibits significantly improved activity and durability for NO₃⁻ removal compared to Cu₂O/Cu. The percentage of NO₃⁻ removal keeps at ∼95.0% with the initial concentration of 60 mg•L⁻¹ NO₃⁻-N at -0.9 V <em>vs.</em> RHE in 15 consecutive cycling tests (corresponding to 30 hours). This work presents a feasible strategy to resist oxidation reconstruction of Cu^(I)-based eNO₃RR catalysts, making NO₃⁻ removal more effective, more durable, and more sustainable.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"28 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142804646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Surfactants on Inactivation of Bacillus Subtilis Spores by Chlorine","authors":"Tianqi Zhang, María Inés Villalba, Rongjun Gao, Sandor Kasas, Urs von Gunten","doi":"10.1016/j.watres.2024.122944","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122944","url":null,"abstract":"Bacterial spores pose significant risks to human health, yet the inactivation of spores is challenging due to their unique structures and chemical compositions. This study investigated the synergistic effect between surfactants and chlorine on the inactivation kinetics of <em>Bacillus subtilis</em> spores. Two surfactants, cocamidopropyl betaine (CAPB) and cetyltrimethylammonium chloride (CTMA) were selected to investigate chlorine disinfection in absence and presence of surfactants. The concurrent presence of both chlorine and surfactant resulted in a moderate reduction in the lag-phases for spore inactivation and negligible increase in the second-order inactivation rate constants. In contrast, when the spores were pre-exposed to surfactants, the lag-phases decreased by about 50% for both CAPB and CTMA, and the second-order inactivation rate constants during post-chlorination remained constant for CAPB but increased by a factor of 2.3 for CTMA, compared to the control group with phosphate buffer. This synergistic effect became more pronounced with longer surfactant pre-exposure times, reaching its maximum at 3-6 h. The observed synergistic effect suggests that surfactants can potentially enhance the permeability of the coat which is the outmost layer of <em>B. subtilis</em> spores and a primary barrier for chemical disinfectants. Tracing a group of <em>B. subtilis</em> spores sequentially treated with surfactant and chlorine by atomic force microscopy, a significant decrease in compressive stiffness of the spores was observed due to exposure to surfactants, indicating alterations in the coat by surfactants. The trend in reducing compressive stiffness aligned well with the decrease of lag-phases in inactivation kinetics. Furthermore, CTMA was found to inactivate <em>B. subtilis</em> spores through mechanisms different from chlorine. Chlorine primarily inactivated <em>B. subtilis</em> spores before damaging the inner membrane of the spores which plays a crucial role in protecting the genetic material stored in the core of the spores. In comparison, CTMA damaged 22% of the inner membrane for an inactivation efficiency of 99%. A synergistic effect in damaging the inner membrane was observed when applying CTMA and chlorine simultaneously instead of sequentially.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"20 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142797313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced abatement of phenolic compounds by chlorine in the presence of CuO without electrophilic aromatic substitution","authors":"Chenhao Tian, Urs von Gunten, Chao Liu","doi":"10.1016/j.watres.2024.122943","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122943","url":null,"abstract":"It has been demonstrated that chlorine predominately reacts with phenolic compounds through an electrophilic aromatic substitution, yielding chlorinated phenols. Previous studies showed that copper oxide (CuO), a water pipe corrosion product, can catalytically enhance the reactivity of chlorine and its disproportionation. In this study, kinetics and mechanisms for the reactions of chlorine with phenolic compounds in the presence of CuO were investigated. CuO at 100 mg/L increases the apparent second-order rate constants (<em>k</em>_app) for reactions of chlorine with phenol, chlorophenols, bromophenols, iodophenols, 2,6-dimethylphenol, acetaminophen, and 4-hydroxybenzoic acid at pH 7.6 and 21°C by up to 50 times. For the same reaction conditions, increasing CuO concentrations from 0 to 200 mg/L increase the <em>k</em>_app of phenol chlorination from ∼42 to 608 M⁻¹ s⁻¹. In general, a stronger enhancement of the chlorine reactions with phenols was observed in the pH range of 6.6-7.6 than 7.6-9.0, indicating that CuO more readily activates hypochlorous acid. Moreover, CuO significantly changes the pathway for phenol chlorination. Yields of chlorophenols decreased from 98% to &lt; 5% as the CuO concentration increased from 0 to 100 mg/L. Non-chlorinated compounds (e.g., catechol, 2,3-dihydroxymuconic acid, maleic acid, and oxalic acid) are major transformation products. Model simulations suggest a pre-equilibrium step with the formation of a CuO-HOCl complex as the rate-limiting step for the overall reactions. Heterogeneous chlorination processes with limited formation of chlorinated phenols tend to be predominant for CuO concentrations &gt; ∼ 5 - 36 mg/L for various phenols. These findings have implications for the transformation of phenolic compounds during chlorination in copper-containing water distribution systems.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"15 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142797690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammoniated-Driven Green Synthesis of Charged Polyoxometalate Supported Ionic Liquids for Exceptional Heavy Metal Remediation in Actual Industrial Wastewater","authors":"Shuo Wang, Xilin Geng, Ziyi Zhao, Manyu Zhang, Yaran Song, Keju Sun, Qingrui Zhang","doi":"10.1016/j.watres.2024.122939","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122939","url":null,"abstract":"Reducing toxic metal concentrations to extremely low levels has long posed a challenge. Polyoxometalate supported ionic liquids (POM-SILs) offer significant potential for advanced water remediation, but their application is limited by complex preparation, toxic solvents, and poor stability due to leaching, compromising sustainability. We introduced a sustainable approach for selectively removing Pb(II) in complex electroplating wastewater using charged POM-SILs composite, synthesized by directly grafting lacunary Keggin ions ([α-SiW₁₁O₃₉]⁸⁻, SiW₁₁) onto charged ammoniated polystyrene via a straightforward, solvent-free process. These POM-SILs featured monodisperse nanoclusters (&lt;5 nm) in a cross-linked polymer matrix, ensuring optimal site accessibility and enhanced stability with negligible leaching. They achieved exceptional Pb(II) selectivity, boasting a distribution coefficient (<em>K</em>_d) of 23,605 mL g⁻¹-over 120 times greater than conventional ion-exchange resins-and a Pb(II) removal efficiency exceeding 97.6%, even in high-salinity, diverse heavy metal environments. They reached a large Q_0.01 value of 0.371 mg g⁻¹, effectively treating up to 2,200 liters of wastewater per kg composite, reducing Pb(II) concentrations to below 0.01 mg L⁻¹, meeting drinking water standards. This method marks a substantial advancement in heavy metal remediation, offering an efficient and sustainable solution for industrial wastewater treatment.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"16 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbes drive more carbon dioxide and nitrous oxide emissions from wetland under long-term nitrogen enrichment","authors":"Yisong Feng, Yanyu Song, Mengyuan Zhu, Mengting Li, Chao Gong, Shouyang Luo, Wenkai Mei, Huanhuan Feng, Wenwen Tan, Changchun Song","doi":"10.1016/j.watres.2024.122942","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122942","url":null,"abstract":"Wetlands are frequently regarded as weak carbon dioxide (CO₂) sinks, the largest natural sources of methane (CH₄), and weak sources of nitrous oxide (N₂O). Anthropogenic activities and climate change-induced nitrogen (N) enrichment may affect wetland carbon (C) and N cycling via soil microbes, consequently modifying the original greenhouse gas (GHG) emissions. However, the effects and mechanisms of the duration and rate of N inputs on wetland GHG emissions remain uncertain and controversial. Therefore, this study conducted an in situ field experiment to investigate the effects and driving mechanisms of long-term N enrichment on wetland GHG emissions throughout the 2023 growing season by using the static opaque chambers method. Soil microbial composition and function were also analyzed through metagenomic sequencing. The results showed that N enrichment significantly increased wetland CO₂ emissions, which were associated with the abundance of microbial C-fixing functional genes and the soil C content. Although nitrogen enrichment tended to suppress CH₄ emissions, the effect was not significant. High N enrichment created a powerful wetland N₂O source driven by the abundance of microbial nitrification function genes and microbial species. Vegetation influenced wetland GHG emissions by altering soil carbon content. This study elucidates the response mechanism of wetland GHG emissions to long-term nitrogen enrichment, thereby furnishing a theoretical basis for wetland conservation and nitrogen management.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"37 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142797683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing electro-functionalized gravity-driven ceramic membrane filtration (EGDCM) for decentralized treatment of algae-laden brackish water: comparison of in-situ electro-oxidation and ex-situ electro-coagulation","authors":"Dachao Lin, Xinxu Shen, Caiwei Tan, Han Zhang, Rui Chen, Xing Du, Heng Liang","doi":"10.1016/j.watres.2024.122940","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122940","url":null,"abstract":"Algae-laden brackish water (ABW) has remarkably threatened drinking water safety in warm coastal areas. Although gravity-driven ceramic membrane filtration (GDCM) exhibits high potential in ABW treatment during decentralized water supply, membrane fouling is still a critical problem. Herein, GDCM was skillfully electro-functionalized (EGDCM) by <em>in-situ</em> electro-oxidation (ISEO) based on self-fabricated Ti/SnO₂-Sb dimensionally stable anode (DSA) (EO-EGDCM) and <em>ex-situ</em> electro-coagulation (ESEC) based on iron anode (EC-EGDCM) in this study. EO-EGDCM with KMnO₄ augment (MnEO-EGDCM) was also established for comparison. Results show that ISEO increased GDCM membrane permeability by 22%, while EC-EGDCM membrane flux was nearly 4.8 times that of GDCM. ISEO enhanced the early removal of organic pollution, and KMnO₄ facilitated the active chlorine oxidization of ammonia and algal toxins on electrified Ti/SnO2-Sb DSA by suppressing the transformation of free chlorine to less reactive chloramines. Both algae cell permeabilization and intracellular organic matter release were enhanced by ISEO. But SEM-EDS, CLSM and biomass analysis evidenced that membrane biological process, which was improved by 32%∼323% by electrical stimulation, developed porous structures in the fouling layer in EO-EGDCM/MnEO-EGDCM. According to energy consumption and carbon emissions evaluation, GDCM was confirmed as an energy-saving system for treating ABW with the consumption of only 3.47×10⁻³ kWh/m³. Electricity demand was increased for EGDCM but still considerably lower than that for other algae-laden water treatment processes. EC-EGDCM reduced energy consumption and carbon emission by around 80% compared to EC-EGDCM/MnEO-EGDCM. Electro-functionalization was a promising option to improve GDCM treatment of ABW via multiple mechanisms but further optimization was still required.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"20 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium-organic matter fouling in nanofiltration: Synchrotron-based X-ray fluorescence and absorption near-edge structure spectroscopy for speciation","authors":"Tyler A. Malkoske, Yang-Hui Cai, Sharon E. Bone, Andrea I. Schäfer","doi":"10.1016/j.watres.2024.122938","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122938","url":null,"abstract":"Calcium (Ca)-enhanced organic matter (OM) fouling of nanofiltration (NF) membranes leads to reduced flux during desalination and requires frequent cleaning. Fouling mechanisms are not fully understood, which limits the development of targeted fouling control methods. This study employed synchrotron-based X-ray fluorescence (XRF) and X-ray absorption near-edge structure (XANES) spectroscopy to quantify the spatial distribution and mass of Ca deposition as well as changes in the Ca coordination environment characteristic of specific fouling mechanisms, respectively. Bench-scale filtration experiments were performed using feed solutions containing Ca and ten different types of organic matter (OM), as well as the common scalants, calcium carbonate (CaCO₃) and calcium sulfate (CaSO₄). Osmotic backwash (OB) was performed at regular intervals for fouling control. Ca-OM aggregation resulted in greater flux decline and lower flux recovery during OB than Ca conditioning of membranes followed by filtration of feed solution with OM. Linear combination fitting (LCF) of XANES absorption spectra from fouled membranes indicated that Ca-OM aggregation preferentially occurred for OM types that exhibited both high carboxylic group and negative charge density. Consequently, these OM types exhibited greater deposition of Ca and TOC on the membrane surface when compared to other OM types. For the coexistence of scalants and OM, Ca speciation within the fouling layer was characteristic of both Ca bound to the membrane (i.e. potential bridging, charge screening) as well as Ca-OM aggregation and deposition mechanisms, while a range of crystal polymorphs were observed to occur simultaneously. XRF and XANES represent powerful tools for the elucidation of NF fouling mechanisms by quantification of Ca deposition as well as Ca speciation. Fouling control methods should target OM types with high carboxyl group density and negative charge to neutralize or eliminate interactions with Ca.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"4 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Selective Removal of PFAS at Trace Level Using Quaternized Cellulose-Functionalized Polymer Resin: Performance and Mechanism","authors":"Jinjing Huang, Kaixing Fu, Zhuoya Fang, Jinming Luo","doi":"10.1016/j.watres.2024.122937","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122937","url":null,"abstract":"The effective protocol for treating per- and polyfluoroalkyl substances (PFAS) in water at environmentally relevant concentrations (∼ ng L⁻¹) has received unprecedented attention due to the stringent drinking water standards. In order to ensure safe water treatment, sorption using quaternary ammonium functionalized strong-base anion exchange resins (SB-AERs) is considered a viable option for treating PFAS when compared to commercialized activated carbon, as SB-AERs can be <em>in situ</em> regenerated with long-term operation capabilities. However, the harsh conditions required for traditional direct synthesis of SB-AERs (such as prolonged reaction times, complex processes, and environmental pollution caused by the organic reagents used) limit their applications. In this study, we present a novel indirect synthesis method that can effectively pre-functionalizes cellulose for quaternization. This modified cellulose is subsequently loaded onto chloromethylated polystyrene to produce a quaternized cellulose-functionalized polymer resin (QC-CMPS). The process is straightforward to implement, reduces the use of toxic chemicals, and effectively mitigates water safety risks associated with hazardous reagent leaks. As results, the prepared QC-CMPS demonstrates exceptional selective capability for PFAS removal in real environmental water matrices, achieving over 99% removal efficiency at an initial concentration of 1000 ng L⁻¹. Additionally, QC-CMPS demonstrates low sensitivity to pH and background ions, effectively removing PFAS from both tap water and lake water with efficiency rates exceeding 95%. The exhausted QC-CMPS can be readily rejuvenated by rinsing with 1% NaCl and MeOH (V/V=3/7) mixture, as demonstrated by five successful consecutive cycles. The fixed-bed column test confirmed that ∼ 11,000 bed volumes (∼ 292.70 L) of the feed streams (∼250 ng L⁻¹) can be effectively treated with the enrichment factor of 26.42, 25.16, 27.77 and 21.17 for PFOA, PFOS, PFBS, and GenX, respectively, highlighting significant potential for practical applications.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"18 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micropollutants removal from aquaculture water using layer-by-layer self-assembled nanofiltration membranes","authors":"Bowen Zhou, Juan Luo, Meng Jin, Ning Xue, Rongrong He, Wei Li, Tao He","doi":"10.1016/j.watres.2024.122933","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122933","url":null,"abstract":"Micropollutants (MPs) in aquaculture water are directly related to human health, but largely overlooked. The conventional water treatment technologies could not effectively remove MPs, and new technologies have been pursued with high MP removal rate, low cost and small footprint. This paper reported the first endeavor of using layer-by-layer (LBL) hollow fiber (HF) nanofiltration (NF) membranes to treat real aquaculture water. We compared three types of membranes with various surface chemical properties and pore sizes assembled by different polyelectrolytes (PEs). The MPs removal rates and operation stability of three NF membranes were studied in flocculation (Floc.)/ultrafiltration (UF)/NF, Floc./NF and direct NF processes. The best poly(styrene sulfonate)/poly(allylamine hydrochloride) (PSS/PAH) NF membrane showed almost 100% MPs removal in Floc./UF/NF, and above 95% in direct NF process. Stable permeance was observed in three 24 h cycles for three processes. The correlation hot spot analysis between the physical properties and removal rates of MPs confirmed importance of size exclusion as the important factor for removal of MPs. The organic humus, rather than inorganic silicon and calcium, was found as the main foulant, which increased slightly the pore size of the LBL membranes with small pores, but blocked pores of membranes with large pores, affected the rejection for ions as well. The cost assessment based on (PSS/PAH)_2.5 showed a total cost below 0.75 ¥/ton water, which is feasible to efficiently remove MPs from aquaculture water by NF membrane process.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"20 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Earth's most needed uncultivated aquatic prokaryotes","authors":"Sophie A. Simon, Vera Aschmann, Annika Behrendt, Michael Hügler, Lisa M. Engl, Marion Pohlner, Sönke Rolfes, Thorsten Brinkhoff, Bert Engelen, Martin Könneke, Luis M. Rodriguez-R, Till L.V. Bornemann, Julia K. Nuy, Louisa Rothe, Tom L. Stach, Kristina Beblo-Vranesevic, Stefan Leuko, Katharina Runzheimer, Ralf Möller, Marius Conrady, Alexander J. Probst","doi":"10.1016/j.watres.2024.122928","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122928","url":null,"abstract":"Aquatic ecosystems house a significant fraction of Earth's biosphere, yet most prokaryotes inhabiting these environments remain uncultivated. While recently developed genome-resolved metagenomics and single-cell genomics techniques have underscored the immense genetic breadth and metabolic potential residing in uncultivated <em>Bacteria</em> and <em>Archaea</em>, cultivation of these microorganisms is required to study their physiology via genetic systems, confirm predicted biochemical pathways, exploit biotechnological potential, and accurately appraise nutrient turnover. Over the past two decades, the limitations of culture-independent investigations highlighted the importance of cultivation in bridging this vast knowledge gap. Here, we collected more than 80 highly sought-after uncultivated lineages of aquatic <em>Bacteria</em> and <em>Archaea</em> with global ecological impact. In addition to fulfilling critical roles in global carbon, nitrogen, and sulfur cycling, many of these organisms are thought to partake in key symbiotic relationships. This review highlights the vital contributions of uncultured microbes in aquatic ecosystems, from lakes and groundwater to the surfaces and depths of the oceans and will guide current and future initiatives tasked with cultivating our planet's most elusive, yet highly consequential aquatic microflora.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"46 47 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}