Galen Medical Journal最新文献

{"title":"Adult Patient Satisfaction with Nursing Care Services and Associated Factors Among Admitted Patients at Saint Paul’s Hospital, Millennium Medical College, Addis Ababa, Ethiopia, 2022: A Cross-Sectional Study","authors":"Bantalem Amanu Bogale, Sindew Mahmud Ahmed, Aynie Birhane Gebrekidan, Getachew Amanu Bogale","doi":"10.31661/gmj.v12i.2906","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2906","url":null,"abstract":"Background: Providing comprehensive nursing care and ensuring patient satisfaction are essential health performance indicators worldwide. Despite some efforts to improve patient satisfaction with nursing care, the approach in developing countries, including Ethiopia, remains insufficient. This study aimed to assess the level of adult patient satisfaction and identify the factors affecting satisfaction. Materials and Methods: This cross-sectional study included 407 participants selected using a simple randomization technique. The samples were distributed using proportional allocation to each selected adult inpatient department. The participants were interviewed using a modified structured Amharic version of the Newcastle Satisfaction with Nursing Scale. Bivariate and multivariable logistic regression analyses were also performed. Results: The overall level of patient satisfaction with nursing care services was 54.3%. Respondents without formal education (P=0.010), male sex (P=0.041), free service consumers (P<0.001), and health insurance users (P<0.001) were significantly associated with satisfaction with nursing care. In addition, previously hospitalized patients (P=0.001), governmental workers (P<0.001), and patients admitted to the medical ward (P=0.010) were associated with patient dissatisfaction with nursing care services.Conclusion: This study revealed that adult patient satisfaction with nursing care services is low. A previous admission history, higher education level, paying cash for services, and private and governmental workers were significant predisposing factors for dissatisfaction with nursing care. On the other hand, patients without formal education, free-service consumers, and male sex were significant predictors of satisfaction with nursing care services. Therefore, hospital administrators are encouraged to focus on patients’ needs and expectations.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135969484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison Between the Effects of Transfer Energy Capacitive and Resistive Therapy and Therapeutic Ultrasound on Hamstring Muscle Shortness in Male Athletes: A Single-Blind Randomized Controlled Trial","authors":"Haniyeh Choobsaz, Nastaran Ghotbi, Poria Mohamadi","doi":"10.31661/gmj.v12i.2981","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2981","url":null,"abstract":"Background: Transfer energy capacitive and resistive (TECAR) therapy (TT) is a newly developed deep heating therapy that can generate heat within tissues through high-frequency wave stimulation. Compared to conventional physiotherapy methods, the application of TT especially in sports rehabilitation is becoming more popular. This study aimed to investigate the comparative effect of TT and therapeutic ultrasound (US) on hamstring muscle shortness. Additionally, the effects of TT with static stretching (SS) were compared with US combined with SS.Materials and Methods: Totally, 39 male athletes with hamstring shortness were randomly assigned into three groups: A, B, and C. Group A received 15 minutes of TT plus SS, while Group B received 15 minutes of US with SS, and Group C only performed SS. Hamstring flexibility was measured by active knee extension (AKE), passive knee extension (PKE), and the sit and Reach (SR) tests before the intervention, and following the first, and third treatment sessions.Results: The range of motion of the AKE and PKE, and displacement range in the SR test improved significantly after the first and third sessions in all three groups (P<0.0001). The improvement of the three flexibility indices in the TT group was greater than in the other two groups.Conclusion: The present study showed that TT could increase the flexibility of hamstring muscles more than US therapy. However, TT in combination with SS had a similar effect to SS alone.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135969486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nursing Interventions and Care Strategies for Patients with Coronary Heart Disease: A Comprehensive Review","authors":"Yangyan Shan, Jun Chen, Siwen Zhou, Guangxue Wen","doi":"10.31661/gmj.v12i.2994","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2994","url":null,"abstract":"Cardiovascular diseases are a major cause of death worldwide, and coronary heart disease (CHD) is a prevalent cardiovascular condition and a significant health burden for the population. In this disease, insufficient blood flow to the heart due to plaque accumulation in the coronary arteries causes chest pain, heart attack, and even death. So, it is vital to identify risk factors, prevention, appropriate treatment, and rehabilitation. Nurses play an indispensable role in managing and caring for patients with CHD. Indeed, they possess a deep understanding of the disease and its complexities, enabling them to provide comprehensive care to patients. Nurses monitor vital signs, administer medications, and perform diagnostic tests, ensuring patients receive timely and appropriate interventions. They also educate patients and their families about CHD, emphasizing lifestyle modifications, medication adherence, and self-care practices. Moreover, nurses offer emotional support, guiding patients through the physical and psychological challenges associated with CHD. Their expertise, compassion, and dedication significantly improve patient outcomes and overall quality of life. Nurses are responsible for assessing, diagnosing, and counseling patients on how to manage their disease, making them the front line of defense in preventing and addressing this serious condition. In the current study, we reviewed the literature to consider the best practices and emerging trends in nursing interventions and care strategies for patients with CHD.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135969485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer Activity of Postbiotic Mediators Derived from Lactobacillus Rhamnosus GG and Lactobacillus Reuteri on Acute Lymphoblastic Leukemia Cells","authors":"Morteza Banakar, Shahroo Etemad-Moghadam, Roza Haghgoo, Majid Mehran, Mohammad Hossein Yazdi, Hadiseh Mohamadpour, Mahdiyar Iravani Saadi, Mojgan Alaeddini","doi":"10.31661/gmj.v12i.3096","DOIUrl":"https://doi.org/10.31661/gmj.v12i.3096","url":null,"abstract":"Background: Leukemia remains a global health challenge, requiring the exploration of alternative therapies with reduced side effects. Probiotics, particularly Lactobacillus species, have gained attention because of their potential anticancer properties. This study investigated the anticancer and cytotoxic effects of postbiotic mediators (PMs) derived from Lactobacillus rhamnosus GG (LGG) and Lactobacillus reuteri (LR) on acute lymphoblastic leukemia (ALL) cells and peripheral blood mononuclear cells (PBMCs). Materials and Methods: The PMs were prepared by culturing LGG and LR strains and isolating the supernatant. The MTT assay assessed cell viability on ALL Jurkat cells and PBMCs, and apoptosis analysis was conducted using flow cytometry. Quantitative real-time PCR was also performed to analyze BAX, BCL-2, BCLX, FAS, and p27 gene expression levels. Results: The results showed that PMs derived from LGG and LR significantly reduced cell viability in Jurkat cells (P<0.05) but not PBMCs (P>0.05). Apoptosis analysis revealed an increase in apoptotic cells after PMs treatment. Nevertheless, gene expression analysis revealed no statistically significant difference between the treated and untreated groups in BAX, BCL-2, BCLX, FAS, and p27 gene expression levels (P>0.05). Conclusion: Findings suggest that specific PMs derived from LGG and LR possess anticancer properties against ALL cells. This research highlighted the promise of PMs as a cutting-edge and less toxic adjuvant therapeutic strategy in cancer treatment.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135969483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Kelussia odoratissima and Angelica sinensis similarities in zebrafish-based in-vivo bioactivity assays and their chemical composition","authors":"Mohammad Rezaei, Parisa Fooladi, Mohamad Norani, Alexander Crawford, Shahram Eisa-Beygi, Yaser Tahamtani, Mahdi Ayyari","doi":"10.31661/gmj.v12i.2793","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2793","url":null,"abstract":"Background: Kelussia odoratissima (KO) and Angelica sinensis (AS) have been used in their indigenous traditional medicine, for various diseases. This study was conducted to evaluate the volatile oil composition of KO leaves (KVL) and AS root (AVR) and biological activity of essential oils (EOs) and hydroalcoholic extracts of both plants using two different transgenic zebrafish (Danio rerio) models.Materials and Methods: Both EOs were isolated by hydrodistillation and analysed by GC and GC/MS. For viability tests, larvae were treated with different concentrations of extracts to determine an appropriate starting concentration. Hydroalcoholic extracts and EOs have been tested in a dose-dependent manner for their biological activity using tissue-specific transgenic zebrafish Tg(fli-1: EGFP) and Tg (ins: GFP-NTR) embryos and larvae. One-way ANOVA was used to compare the mean of pBC area and intersegmental vessels (ISVs) outgrowth between the treatment groups.Results: Eleven compounds were in common to both oils, comprising 51.3% of KVL and 61.7% of AVR, of which 39.3% in KVL and 37.6% in AVR were phthalide structures. Results revealed that both EOs blocked ISVs formation in the Tg (fli-1: EGFP) embryos increased to 10% of the control value, while both hydroalcoholic extracts did not show any anti-angiogenesis effects in these embryos. In addition, AVR has been shown to significantly induce PBC regeneration following ablation in the Tg (ins: GFP-NTR), but its regenerative activity was lower than that of 5′-N-ethylcarboxamidoadenosine (NECA) as a positive control. Taken together, the anti-angiogenesis activity of both EOs could be attributed to the phthalide structures while for the PBC regenerative activity, other compounds including β-Thujaplicinol, exclusively existing in AVR, might be effective.Conclusion: Although the genera, organs, and origin of these plants are different, their similar chemical composition and biological activities make them valuable resources for further investigation in basic medical and pharmaceutical science.&#x0D; &#x0D;","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135398502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Association of Dietary Linoleic Acid and Alpha-linolenic Acid with Adipose Tissue in a Sample of Iranian Adults; A Cohort-based Cross-sectional Study","authors":"Esmail Karami, Saeid Hadi, Mohsen Mohit, Seyed Jalil Masoumi","doi":"10.31661/gmj.v12i.3023","DOIUrl":"https://doi.org/10.31661/gmj.v12i.3023","url":null,"abstract":"Background: Overweight and obesity are the most critical risk factors for chronic diseases. The quality of dietary fatty acids as one of the factors affecting fat accumulation has received little attention. This study investigates the association between dietary linoleic acid (LA) and alpha-linolenic acid (ALA) with body fat indices in a sample of healthy Iranian adults.Materials and Methods: In this cohort-based cross-sectional study, 3,195 individuals aged 20 to 60 who participated in the Shiraz University of Medical Science Employees Health Cohort study were included. Dietary intake was assessed using a validated 118-item Food Frequency Questionnaire (FFQ), and body composition was assessed by the bioelectrical impedance analysis method. Multiple linear regression adjusted for relevant confounders was used to determine the associations.Results: Mean dietary intake of LA was 14.20 ± 7.01 mg/day for men and 13.90 ± 6.71 mg/day for women. Additionally, the daily intake of ALA was 0.18 ± 0.18 mg/day in men and 0.17 ± 0.19 mg/day in women. Dietary intake of ALA for men had an inversely significant association with body fat mass (BFM) (β: -0.585, 95% CI: -1.137, -0.032, P=0.038), percentage of body fat (PBF) (β: -0.537, 95% CI: -0.945, -0.129, P=0.010), Visceral Fat Area (VFA) (β: -2.998, 95% CI: -5.695, -0.302, P=0.029), and Waist to Hip Ratio (WHR) (β: -0.689, 95% CI: -1.339, -0.040, P=0.038).Conclusion: Higher dietary ALA intake was associated with lower BFM, BFP, VAF, and WHR in men. The present study confirms that ALA intake should be considered a preventive treatment to improve body composition. However, further research is recommended in this regard.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134919320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABO/Rh Blood Groups and the Temperament (Mizaj) Based on Traditional Persian Medicine Perspective: A Cross-sectional Study","authors":"M. Parvizi, Mohadeseh Molayemat, M. Rezaee, B. Shirazi Yeganeh, T. Rampp, M. H. Hashempur","doi":"10.31661/gmj.v12i.3062","DOIUrl":"https://doi.org/10.31661/gmj.v12i.3062","url":null,"abstract":"Background: Mizaj is a fundamental concept in traditional Persian medicine (TPM) that explains the physiological and psychological traits of human beings. This study aimed to investigate the association between the ABO/Rh blood groups and the Mizaj.Materials and Methods: This cross-sectional study was conducted on 18-60 years old individuals who visited the Iranian Blood Transfusion Organization, Shiraz Branch. We used Mojahedi’s Mizaj questionnaire to determine the Mizaj of the participants. The participants’ blood group and Rh were determined based on the antibodies against types A, B, and Rh antigens of their blood. Results: We assessed 308 individuals consisting of 174 (56.49%) male and 134 (43.51%) female. The results of our study showed a significant relationship between the AB blood group and the cold Mizaj in both simple and compound Mizaj categories (P=0.012, P=0.014, respectively). Logistic regression analysis revealed that people with the AB blood group were 2.88 times more likely to have cold Mizaj than people with the O blood group (OR=2.88, 95% CI: 1.16 to 7.15). In addition, those with the A blood group were found to be 54% less likely to have a dry Mizaj than those with the O blood group (OR=0.46, 95% CI: 0.23 to 0.92). There was no significant association between the participants’ Mizaj and their Rh blood group (P>0.05).Conclusion: It seems that there was a significant relation between the AB blood group and cold Mizaj, as well as the A blood group and dry Mizaj. However, further studies are recommended with controlling the probable confounding factors.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80243328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Smartphone-Based Video Directly Observed Therapy to Increase Tuberculosis Medication Adherence: An Interventional Study.","authors":"Manal M Al Daajani, Abdullah J Alsahafi, Abdullah M Algarni, Abdulhamed L Moawwad, Ahmed A Osman, Khalid Y A Algaali, Mohammed Abdalaziz, Muhammad A Halwani, Shrooq M Aldajani, Nazik M H Mohammed, Heassah S Alshamrani, Mohammed N Alshahrani, Ghadah M Albostani, Naif G Alshammari, Rami S Alzahrani, Saadiya O Alsomali, Ibrahim Assiri","doi":"10.31661/gmj.v12i.3067","DOIUrl":"10.31661/gmj.v12i.3067","url":null,"abstract":"<p><strong>Background: </strong>Tuberculosis (TB) treatment through Directly Observed Therapy (DOT) has an alternative form of video surveillance therapy (VOT) that utilizes the technological capabilities of smartphones to provide patients with low-cost access to doctors without impacting their work and personal life. We aimed to assess TB patients' drug compliance, perceptions, and feasibility towards smartphone-based video direct observed therapy (VDOT) in Jeddah, KSA.</p><p><strong>Materials and methods: </strong>We conducted a prospective non-randomized interventional study. We delivered smartphone-based VDOT among previously unstudied patients to monitor adherence to the treatment regimen. The expected total number of VDOT sessions was1200. We conducted post-intervention interviews to assess acceptability and satisfaction.</p><p><strong>Results: </strong>In this study, we included 20 participants, 16 of whom were males, with a mean age of 34.3 (±12.5) years. No side effects to the treatments were identified in all participants. The adherence rate for the total period was 93% and 99.5%, measured by the first and second methods, respectively. Most participants were satisfied with the VDOT experience, the time spent on sessions, and the approach's privacy.</p><p><strong>Conclusion: </strong>This study provides promising results for the feasibility and effectiveness of smartphone-based VDOT for TB treatment to increase adherence which was indicated by a high compliance rate, acceptability, and high satisfaction level.</p>","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86664361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Small Genetic Variants in Breast Cancer Cell Line Under Tamoxifen Therapy","authors":"Mahnaz Nezamivand-Chegini, Hamed Kharrati-Koopaee, Seyed Taghi Heydari, Hassan Giahi, Fatemeh Sabahi, Ali Dehshahri, Kamran Bagheri Lankarani","doi":"10.31661/gmj.v12i.2598","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2598","url":null,"abstract":"Background: Tamoxifen (TAM) is an effective hormone therapy that reduces the risk of cancer recurrence. According to evidence, TAM contributes to the alterations of genetic variants background and plays a role in the effectiveness of treatments via alteration of the genetic variants. The effects of TAM on genomic features were investigated in the current study by discovering genetic variants and finding the answer to the following question: “Is there any association between the alterations of genetic variants under TAM consumption and an effective treatment process?” Materials and Methods: Whole-transcriptome (RNA-seq) dataset from four investigations including 10 TAM-treated samples and 9 untreated samples as the control groups were derived from European Bioinformatics Institute (EBI). Using the process of variants calling, the differential genetic variants between and gene ontology enrichment analysis were detected by CLC Genomics Workbench (12). Results: Current study reported about 5.8 million genetic variants. The outcomes of the chi-square test showed that distributions of genetic variants between control and treated samples were significant (p<0.05). The genetic variants comparison between the control and TAM-treated samples indicated that there were 67 differential genetic variants. Gene ontology enrichment analysis indicated that differential genetic variants were associated with several tumor suppressors and oncogenes including IL6ST, GEN1, FNTA. HSPA5, NSMCE2, and DDX11.Conclusion: Most of the candidate genes with different genetic variants had dual roles as oncogenes or tumor suppressors. Therefore, it can be argued that TAM does not play a significant role in an effective treatment through alteration of the genetic variants. In other words, it cannot be concluded that the TAM therapy-resulted alterations of genetic variants play a positive or negative role in the treatment process.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135746706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic and Apoptotic Effects of Vanadyl Sulfate on MCF-7 Breast Cancer Cell Line.","authors":"Maryam Dehdashti, Zahra Abbasy, Hamid Zaferani Arani, Hesam Adin Atashi, Seyed Alireza Salimi-Tabatabaee, Afsaneh Ghasemi, Zhila Fereidouni, Hadi Zare Marzouni, Habib Zakeri, Seyed Abbas Mirmalek","doi":"10.31661/gmj.v12i.3050","DOIUrl":"10.31661/gmj.v12i.3050","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer (BC) is the major cause of cancer-related death in women. Some studies have indicated the cytotoxic effects of vanadyl oxide sulfate (VOSO4). This study aimed to evaluate the anti-cancer effect of VOSO4 in the treatment of MCF-7 cell lines.</p><p><strong>Materials and methods: </strong>The MCF-7 cell line was treated with different concentrations of VOSO4 for 24 and 48 hours. Cell death was measured using the MTT assay. The cell apoptosis rate was measured using Annexin V/Propidium Iodide assay through flow cytometry. Also, the expression levels of p53, P21, Caspase8, superoxide dismutase type 1 (SOD1), Sod2, and Bcl2 mRNAs were assessed, and Western blotting was performed for Sod1 protein.</p><p><strong>Results: </strong>The results showed that the half-maximal inhibitory concentration (IC50) for VOSO4 was 25 and 20 μg/ml for 24 and 48 hours, respectively. Indeed, VOSO4 has dose-dependent cytotoxic effects on the MCF-7. Also, after exposure to VOSO4 for 24 hours, cell apoptosis reached 52% compared with untreated cells. Moreover, after 24 hours of exposure to VOSO4 with IC50 concentration, the expression of p53, P21, Caspase8, Sod1, and Sod2 mRNAs increased (P0.05), and the expression of Bcl2 mRNA was decreased (P0.05). Also, the Western blotting revealed Sod1 protein level markedly increased following exposure to VOSO4 (P0.05).</p><p><strong>Conclusion: </strong>Our results demonstrated that VOSO4 has an apoptotic and cytotoxic effect on BC cells. Therefore, it could be considered a complementary agent for the medical treatment of patients with BC.</p>","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78753389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}