Microbiology Research最新文献_第9页

Isolation and Characterization of Lactic Acid Bacteria from Cocoa Mucilage and Meat: Exploring Their Potential as Biopreservatives for Beef 从可可黏液和肉类中分离和鉴定乳酸菌:探索其作为牛肉生物防腐剂的潜力

Microbiology Research Pub Date : 2023-08-18 DOI: 10.3390/microbiolres14030077

Laura Morante-Carriel, Fernando Abasolo, Carlos Bastidas-Caldes, Erwin A. Paz, Rodrigo Huaquipán, Rommy Díaz, Marco Valdes, David Cancino, Néstor Sepúlveda, John Quiñones

{"title":"Isolation and Characterization of Lactic Acid Bacteria from Cocoa Mucilage and Meat: Exploring Their Potential as Biopreservatives for Beef","authors":"Laura Morante-Carriel, Fernando Abasolo, Carlos Bastidas-Caldes, Erwin A. Paz, Rodrigo Huaquipán, Rommy Díaz, Marco Valdes, David Cancino, Néstor Sepúlveda, John Quiñones","doi":"10.3390/microbiolres14030077","DOIUrl":"https://doi.org/10.3390/microbiolres14030077","url":null,"abstract":"The aim of this study was to characterize lactic acid bacteria (LAB) isolated from cocoa mucilage and beef and evaluate their inhibitory effect in vitro against pathogenic bacteria, as well as determine their effect on beef quality. For the antagonist assay, 11 strains of LAB were selected and tested against pathogenic strains of Escherichia coli and Salmonella sp. The pathogenic bacteria were cultured in a medium, and a previously reactivated LAB bacterial pellet was added. After incubation, halos were observed around the bacterial colonies of the pathogenic strains, indicating inhibition by the LAB. It was identified that the LAB strains used belonged to the genus Lactobacillus, and the CCN-5 strain showed high percentages of inhibition against Salmonella sp. (58.33%) and E. coli (59%). The effectiveness of LAB application methods (immersion, injection, and spraying) did not present statistical differences. Furthermore, no significant changes in the physicochemical characteristics of beef were observed after the application of LAB. The results obtained demonstrate the potential of cocoa mucilage, as a biological control agent through LAB application, for beef biopreservation due to its ability to inhibit the growth of pathogenic bacteria.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136020402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microcystin Concentrations and Detection of the mcyA Gene in Water Collected from Agricultural, Urban, and Recreational Areas in a Karst Aquifer in the Yucatan Peninsula of Mexico 墨西哥尤卡坦半岛喀斯特含水层农业、城市和休闲用水中微囊藻毒素浓度及mcyA基因检测

IF 1.5

Microbiology Research Pub Date : 2023-08-18 DOI: 10.3390/microbiolres14030078

Gerardo Ávila-Torres, G. Rosiles-González, Víctor Hugo Carrillo-Jovel, G. Acosta‐González, E. Cejudo-Espinosa, Daniela Ortega-Camacho, C. Hernández-Zepeda, Oscar Alberto Moreno Valenzuela

{"title":"Microcystin Concentrations and Detection of the mcyA Gene in Water Collected from Agricultural, Urban, and Recreational Areas in a Karst Aquifer in the Yucatan Peninsula of Mexico","authors":"Gerardo Ávila-Torres, G. Rosiles-González, Víctor Hugo Carrillo-Jovel, G. Acosta‐González, E. Cejudo-Espinosa, Daniela Ortega-Camacho, C. Hernández-Zepeda, Oscar Alberto Moreno Valenzuela","doi":"10.3390/microbiolres14030078","DOIUrl":"https://doi.org/10.3390/microbiolres14030078","url":null,"abstract":"The eutrophication of freshwater ecosystems allows the proliferation of cyanobacteria that can produce secondary metabolites such as microcystins. The main aim of this study was to explore the occurrence and concentration of microcystin and the mcyA gene in water bodies located in agricultural, urban, and recreational areas in the karst aquifer of the Yucatan peninsula of Mexico (YPM) and to analyze the water quality variables and chlorophyll-a (Chl-a) associated with their presence. Water samples were collected from 14 sites, and microcystin concentrations were quantified using antibody-based ELISA test. Total DNA was isolated from filters and used for PCR amplification of a fragment of the mcyA gene. Amplicons were cloned and sequenced to identify toxin-producing cyanobacteria present in water. Results showed that water bodies had different trophic status based on Carlson’s trophic state index. Dissolved inorganic nitrogen (DIN: NH4+ + NO3− + NO2−) and P-PO43− concentrations were within a range of 0.077–18.305 mg DIN/L and 0.025–2.5 mg P-PO43−/L, respectively, per sampled site. All sampled sites presented microcystin concentrations within a range of ≥0.14 µg/L to ≥5.0 µg/L, from which 21.4% (3/14) exceeded the limit established in water quality standards for water consumption (1 µg/L). The mcyA gene fragment was detected in 28.5% (4/14) of the sites. A total of 23 sequences were obtained from which 87% (20/23) shared >95% nucleotide identity (nt) with the genus Microcystis and 13% (3/23) shared >87% nt identity with uncultured cyanobacteria. No correlation with the presence of the mcyA gene and microcystins was found; however, a positive correlation was detected between microcystin concentrations with pH and Chl-a.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"45 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90241016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Novel Emulsion Formulation of Trichoderma asperelloides PSU-P1 Conidia against Stem Canker on Dragon Fruit Caused by Neoscytalidium dimidiatum 曲霉木霉PSU-P1分生孢子抗火龙果茎溃疡病乳剂的研制

IF 1.5

Microbiology Research Pub Date : 2023-08-17 DOI: 10.3390/microbiolres14030076

W. Intana, P. Wonglom, Kim Sreang Dy, A. Sunpapao

{"title":"Development of a Novel Emulsion Formulation of Trichoderma asperelloides PSU-P1 Conidia against Stem Canker on Dragon Fruit Caused by Neoscytalidium dimidiatum","authors":"W. Intana, P. Wonglom, Kim Sreang Dy, A. Sunpapao","doi":"10.3390/microbiolres14030076","DOIUrl":"https://doi.org/10.3390/microbiolres14030076","url":null,"abstract":"Stem canker on dragon fruit caused by Neoscytalidium dimidiatum causes severe losses in production of this fruit worldwide. Biological control by Trichoderma species is widely used to control several plant diseases. However, environmental conditions affect the use of biocontrol agents in the field. The development of a new formulation may offer an alternative way to address the problem of stem canker on dragon fruit caused by N. dimidiatum. In this study, we sought to develop a Trichoderma asperelloides PSU-P1 formulation that would be effective against N. dimidiatum. Three vegetable oils, two emulsifier-dispersing agents (Tween 20 and Tween 80), and one source of carbon (dextrose) were tested for carrier additives. We assessed the viability and antifungal ability of formulations incubated at ambient temperature and at 10 °C during a storage period of 1–6 months. The formulation composed of coconut oil, DW, and tween 20 in a ratio of 30:60:10 required a mixing time of 1.14 min; this was significantly faster than the mixing times of other formulations. Application of this formulation suppressed canker development; a canker area of 0.53 cm2 was recorded, compared with a control (pathogen only) area of 1.65 cm2. In terms of viability, this formulation stored at ambient temperature showed a surface area percentage of T. asperelloides PSU-P1 ranging from 64.43 to 75.7%; the corresponding range for the formulation stored at cool temperature was 70.59–75.6%. For both formulations, percentage inhibition gradually decreased from 1 to 6 months, with ranges of 59.21–77% and 60.65–76.19% for formulations incubated at ambient and cool temperatures, respectively. Our findings suggest that the formulation developed in this study prolongs the viability of T. asperelloides PSU-P1 conidia by up to 6 months, effectively inhibits N. dimidiatum in vitro, and reduces stem canker in vivo.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84904181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial, Antibiofilm, and Anti-Inflammatory Activities of Ginger Extract against Helicobacter pylori 生姜提取物对幽门螺杆菌的抗菌、抗生物膜和抗炎活性

IF 1.5

Microbiology Research Pub Date : 2023-08-16 DOI: 10.3390/microbiolres14030075

Mahmoud K. M. Elbestawy, G. El-Sherbiny, Saad A. Moghannem, Eman E Farghal

{"title":"Antibacterial, Antibiofilm, and Anti-Inflammatory Activities of Ginger Extract against Helicobacter pylori","authors":"Mahmoud K. M. Elbestawy, G. El-Sherbiny, Saad A. Moghannem, Eman E Farghal","doi":"10.3390/microbiolres14030075","DOIUrl":"https://doi.org/10.3390/microbiolres14030075","url":null,"abstract":"The increased emergence of multidrug-resistant Helicobacter pylori is related to many health issues. Zingiber officinale (Z. officinale) is a plant usually used in folk medicine to treat a variety of diseases. This study was conducted to evaluate the ability of Z. officinale extract to combat resistant H. pylori. The disc diffusion, microdilution, and microplate assays were performed to evaluate the susceptibility to antibiotics and the antibacterial and antibiofilm activities of the Z. officinale extracts. Using the checkerboard method, the combined effects of gentamicin and Z. officinale extract were investigated. In addition, anti-inflammatory activity and GC-MS analysis were performed according to a modified protocol. According to the findings, H. pylori isolates exhibited resistance rates of 56.33, 50.0, and 45.85 against metronidazole, gentamicin, and tetracycline, respectively. The methanolic extract of Z. officinale showed the strongest effectiveness against resistant H. pylori isolates with MICs of 20.0 to 50.0 µg/mL, including both H. pylori isolates and the standard strain NCTC 11637. Z. officinale extract suppresses the biofilm formed by H. pylori isolates with a percentage of 92.96% at 50.0 µg/mL, compared with 97.19% for gentamicin at the same concentration. According to FICI values, the combination of methanolic Z. officinale extract with gentamicin increases bacterial sensitivity to such drugs. Moreover, the Z. officinale extract exhibits strong anti-inflammatory activity, with inhibition of red blood cell membrane stabilization increasing from 49.83% to 61.47% at a concentration of 4 to 32 µg/mL. The GC-MS analysis of Z. officinale extract exhibits 17 different chemical compounds. Besides showing antibacterial properties, the extract also contains the anti-inflammatory compound gingerol as the main constituent, which inhibits the growth of H. pylori and its biofilm and is a promising natural therapeutic alternative or enhances antibiotic activity.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"135 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77035083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baicalin Attenuated PANX-1/P2X7 Axis, P2Y6, and NLRP3/Caspase-1 Signaling Pathways in Peritonitis Induced by Glaesserella parasuis 黄芩苷减弱副猪绿脓杆菌所致腹膜炎的PANX-1/P2X7轴、P2Y6和NLRP3/Caspase-1信号通路

IF 1.5

Microbiology Research Pub Date : 2023-08-09 DOI: 10.3390/microbiolres14030074

S. Fu, Xinyue Tian, Jingyang Li, Yuzhen Yuan, Xiaoyi Li, Mingxing Ren, Ling Guo, Chun Ye, Bingbing Zong, Yu Liu, Qirong Lu, Y. Qiu

{"title":"Baicalin Attenuated PANX-1/P2X7 Axis, P2Y6, and NLRP3/Caspase-1 Signaling Pathways in Peritonitis Induced by Glaesserella parasuis","authors":"S. Fu, Xinyue Tian, Jingyang Li, Yuzhen Yuan, Xiaoyi Li, Mingxing Ren, Ling Guo, Chun Ye, Bingbing Zong, Yu Liu, Qirong Lu, Y. Qiu","doi":"10.3390/microbiolres14030074","DOIUrl":"https://doi.org/10.3390/microbiolres14030074","url":null,"abstract":"Glaesserella parasuis (G. parasuis) can cause peritonitis in piglets. However, the pathogenesis of peritonitis remains unclear. Baicalin has been shown to possess anti-inflammatory and anti-oxidant functions. The aim of this study was to investigate the role of the PANX-1/P2X7 axis and the P2Y6 signaling pathway in peritonitis induced by G. parasuis and the effect of baicain on the PANX-1/P2X7 axis and P2Y6 pathway activation triggered by G. parasuis. A G. parasuis serovar 5 isolate SH0165 strain was obtained from the lungs of commercially produced pigs which had the typical symptoms of Glässer’s disease, namely arthritis, fibrinous polyserositis, hemorrhagic pneumonia, and meningitis. Then, 35 piglets were randomly divided into five groups, each group containing seven piglets. The groups consisted of a negative control group, an infection group, a 25 mg/kg baicalin group, a 50 mg/kg baicalin group, and a 100 mg/kg baicalin group. The results showed that G. parasuis could promote PANX-1/P2X7 axis and P2Y6 activation; induce NLRP3/caspase-1, IL-1β and IL-18 expression; trigger PLC/PKC and MLCK/MLC signaling activation; attenuate the expression of tight junction proteins ZO-1, E-cadherin, Occludins, and claudin 1; and stimulate CD14, CD24, CD36, CD47, and CD91 expression in the peritoneum as measured via Western blot (p < 0.01; PLC, p < 0.05). Baicalin could significantly inhibit PANX-1/P2X7 axis, P2Y6, and NLRP3/caspase-1 activation; reduce IL-1β and IL-18 expression; attenuate PLC/PKC and MLCK/MLC activation; promote ZO-1, E-cadherin, occludins, and claudin 1 expression; and reduce CD14, CD24, CD36, CD47, and CD91 expression in the peritoneum induced by G. parasuis as measured via Western blot. Our results deepen the understanding of the mechanism of peritonitis triggered by G. parasuis and provide some novel potential methods of controlling G. parasuis infection.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"20 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86266842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sage and Lavender Essential Oils as Potential Antimicrobial Agents for Foods 鼠尾草和薰衣草精油作为潜在的食品抗菌剂

IF 1.5

Microbiology Research Pub Date : 2023-08-07 DOI: 10.3390/microbiolres14030073

B. Speranza, Angela Guerrieri, Angela Racioppo, A. Bevilacqua, D. Campaniello, M. Corbo

{"title":"Sage and Lavender Essential Oils as Potential Antimicrobial Agents for Foods","authors":"B. Speranza, Angela Guerrieri, Angela Racioppo, A. Bevilacqua, D. Campaniello, M. Corbo","doi":"10.3390/microbiolres14030073","DOIUrl":"https://doi.org/10.3390/microbiolres14030073","url":null,"abstract":"Modern society is becoming more and more reluctant to use antibiotic or chemical compounds in food production and is demanding foods without what they perceive as artificial and harmful chemicals, including many used as antimicrobials and preservatives in food. Another big problem is the improper use of antibiotics, especially broad-spectrum ones, which has significantly contributed to increased antibiotic resistance in many microorganisms. As a consequence, the whole scientific world has recently concentrated numerous studies on the research of natural remedies capable of counteracting multidrug-resistant strains and fighting infections: the use of aromatic plants and their essential oils (EOs) as potential alternatives to conventional antimicrobials to extend shelf life and combat foodborne pathogens has heightened. Among EOs, sage and lavender have also been promoted for their potential antimicrobial capabilities. In this review, we summarize the latest research studies performed about sage and lavender EOs, focusing on their chemical composition and their biological and antimicrobial properties; the aim is to give an overview of the current knowledge about their major components, effectiveness, mechanisms of action, synergistic effects and use in foods to facilitate a widespread application in both food and pharmaceuticals industries.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"30 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83049065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Prospective Study in Evaluating Candida spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One 利用微aut- am和Sensititre Yeast-One评价念珠菌体外敏感性的比较研究

IF 1.5

Microbiology Research Pub Date : 2023-08-07 DOI: 10.3390/microbiolres14030072

L. Trovato, Maddalena Calvo, G. Scalia, S. Oliveri

{"title":"A Comparative Prospective Study in Evaluating Candida spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One","authors":"L. Trovato, Maddalena Calvo, G. Scalia, S. Oliveri","doi":"10.3390/microbiolres14030072","DOIUrl":"https://doi.org/10.3390/microbiolres14030072","url":null,"abstract":"Background. Among invasive fungal infection pathogens, Candida spp. represent the most common aetiological agents. The increasing rate of severe infections and the emergence of antimicrobial resistance highlight the importance of in vitro susceptibility testing. The EUCAST and the CLSI have established reference microdilutions that are reliable but difficult to apply in a laboratory routine. Commercial microdilutions could represent a valuable alternative within a diagnostic workflow. Methods. A number of 50 Candida spp. collected from positive blood samples simultaneously underwent the Sensititre Yeast-One microdilution as a standard susceptibility test and the Micronaut-AM as an experimental method. A comparison between the two techniques was produced, evaluating the effectiveness of the Micronaut-AM compared to the extensively consolidated Sensititre Yeast-One. Results. The two techniques revealed optimal agreement rates, confirming the reliability of the commercial microdilution kits within the diagnostic workflows. The results showed remarkable concordance for both susceptible and resistant isolates, highlighting slight variations in the different identified Candida species. Conclusions. Future studies about antifungal susceptibility testing should be encouraged, including molecular confirmation of possible resistance phenotypes and extended isolate numbers for the different Candida species. Moreover, it would be interesting to plan clinical trials after the execution of the examined commercial microdilution methods.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"53 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89857730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Population Knowledge and Practices and the Prevalence of Trypanosomes Circulating in Domestic Animals in Three Active Human African Trypanosomiasis Foci in the Republic of Congo 刚果共和国三个非洲人类锥虫病活动性疫源地的人口知识和实践以及家畜中锥虫流行情况

IF 1.5

Microbiology Research Pub Date : 2023-08-04 DOI: 10.3390/microbiolres14030071

Irina Bemba, A. Lenga, H. Awono-Ambene, C. Antonio-Nkondjio

{"title":"Population Knowledge and Practices and the Prevalence of Trypanosomes Circulating in Domestic Animals in Three Active Human African Trypanosomiasis Foci in the Republic of Congo","authors":"Irina Bemba, A. Lenga, H. Awono-Ambene, C. Antonio-Nkondjio","doi":"10.3390/microbiolres14030071","DOIUrl":"https://doi.org/10.3390/microbiolres14030071","url":null,"abstract":"Human African Trypanosomiasis (HAT) is still endemic in the Republic of Congo. Although the incidence of cases has significantly decreased over years, the disease still persists in some active foci. Factors contributing to the maintenance of the disease such as the existence of an animal reservoir or population knowledge are still not well known. It is in this context that a study focusing on the knowledge and practices of the population with regard to HAT as well as on the prevalence of trypanosomes infecting animals was undertaken in three active HAT foci in the Republic of Congo. The study was performed using field surveys conducted from November 2019 to June 2021. Domestic animal blood was examined by microscopy and PCR to detect the presence of trypanosomes. A structured questionnaire was administered to the population to assess their knowledge and practices concerning HAT in these endemic foci. More than half of the animals examined were found to be infected with trypanosomes (51.22%). The main trypanosome species infecting animals were Trypanosoma congolense savannah (67.2%) and Trypanosoma brucei (s.l.) (32.8%). No trypanosomes infecting humans were detected. Concerning household surveys, more than half of the respondents (52.9%) were fully aware of the mode of transmission and symptoms of the disease. The majority of people preferred to wear clothes covering the whole body and to use locally made soap as repellents to protect themselves from tsetse fly bites. This study suggests frequent circulation of animal trypanosomes in domestic animals and the use of personal measures to protect against tsetse fly bites. Updating information on the HAT animal reservoir and population knowledge alongside regular monitoring of the tsetse fly populations and the use of traps to control tsetse flies are crucial to drive efforts towards the elimination of gHAT in the Republic of Congo.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"4 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75667995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of Azo Dye Brown 703 by Pseudomonas aeruginosa: An Effective Treatment Technique for Dye-Polluted Wastewater 铜绿假单胞菌生物修复偶氮染料棕703:一种有效的染料污染废水处理技术

IF 1.5

Microbiology Research Pub Date : 2023-08-02 DOI: 10.3390/microbiolres14030070

Asad Ullah Khan, Muhammad Zahoor, Mujaddad Ur Rehman, Muhammad Ikram, Daochen Zhu, Muhammad Naveed Umar, R. Ullah, Essam A. Ali

{"title":"Bioremediation of Azo Dye Brown 703 by Pseudomonas aeruginosa: An Effective Treatment Technique for Dye-Polluted Wastewater","authors":"Asad Ullah Khan, Muhammad Zahoor, Mujaddad Ur Rehman, Muhammad Ikram, Daochen Zhu, Muhammad Naveed Umar, R. Ullah, Essam A. Ali","doi":"10.3390/microbiolres14030070","DOIUrl":"https://doi.org/10.3390/microbiolres14030070","url":null,"abstract":"Dye-polluted wastewater poses a serious threat to humans’, animals’ and plants’ health, and to avoid these health risks in the future, the treatment of wastewater containing dyes is necessary before its release to environment. Herein, a biological approach is used; the textile azo dye brown 703 is degraded utilizing Pseudomonas aeruginosa. The bacterial strain was isolated from textile wastewater dumping sites in Mingora, Swat. The optimization for bacterial degradation was carried out on the nutrient broth medium, which was then subjected to a variety of environmental physicochemical conditions and nutritional source supplementation before being tested. Under micro-aerophilic circumstances, the maximum decolorization and degradation of dye occurred at a 20 ppm dye concentration within 3 days of incubation at a neutral pH and 38 °C. The decrease in the intensity of the absorbance peak in the UV–Vis spectrum was used to measure the extent of decolorization. Initially, 15 bacterial strains were isolated from the textile effluent. Out of these strains, Pseudomonas aeruginosa was found to be the most potent degrading bacteria, with a degradation extent of around 71.36% at optimum conditions. The appearance and disappearance of some new peaks in the FT-IR analysis after the degradation of brown 703 showed that the dye was degraded by Pseudomonas aeruginosa. The GC–MS analysis performed helped in identifying the degraded compounds of azo dye that were utilized in illustrating the under-study process of brown 703 degradation. The biodegradation brought about by Pseudomonas aeruginosa can be employed successfully in the future as an eco-friendly approach with far reaching results.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"895 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86331482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Natural Products from Red Algal Genus Laurencia as Potential Inhibitors of RdRp and nsp15 Enzymes of SARS-CoV-2: An In Silico Perspective Laurencia红藻属天然产物作为SARS-CoV-2病毒RdRp和nsp15酶潜在抑制剂的研究

IF 1.5

Microbiology Research Pub Date : 2023-07-31 DOI: 10.3390/microbiolres14030069

Omkar Pokharkar, Harshavardhan Anumolu, G. Zyryanov, M. Tsurkan

{"title":"Natural Products from Red Algal Genus Laurencia as Potential Inhibitors of RdRp and nsp15 Enzymes of SARS-CoV-2: An In Silico Perspective","authors":"Omkar Pokharkar, Harshavardhan Anumolu, G. Zyryanov, M. Tsurkan","doi":"10.3390/microbiolres14030069","DOIUrl":"https://doi.org/10.3390/microbiolres14030069","url":null,"abstract":"The genus Laurencia, a category of marine red algae, is well recognized for producing a large variety of natural products (NPs) that are both chemically intriguing and structurally distinct. The aim of this research was to identify NPs with potential anti-SARS-CoV-2 activity. The crystals of the proteins RdRp and nsp15 were obtained from the RCSB protein database. About 300 NPs were discovered using the PubChem, ChemSpider, and CMNPD databases. The program Autodock Vina was used to conduct the molecular docking procedure once the proteins and ligands were prepared. Before running MD simulations using the CABS-flex 2.0 website, binding affinity assessments and interactions between amino acids were carefully reviewed. Only nine NPs were shortlisted to be examined further. Bromophycolide R, S, and bromophycoic acid C show the tendency to inhibit RdRp by β-hairpin motif binding at the N-terminal known as Active site 2 (AS2), whereas the other four NPs, bromophycolide E, H, P, and thyrsenol A, may effectively inhibit RdRp through interactions via C-terminal, also known as the Active site 1 (AS1). For the enzyme nsp15, bromophycoic B, C, and floridoside showed plausible interactions. In conclusion, out of nine, seven candidates shortlisted for RdRp exhibited strong interactions with the key residues in the AS1 and AS2 regions. Bromophycoic acid C may work as a dual inhibitor due to its favorable interactions with the nsp15 protein and RdRp’s N-terminal, with affinities of −8.5 and −8.2 kcal/mol, respectively.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"100 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80321153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1