Microbiology Research最新文献_第10页

Evidence of Brucellosis in Hospitalized Patients of Selected Districts of Punjab, Pakistan 巴基斯坦旁遮普选定地区住院患者中布鲁氏菌病的证据

IF 1.5

Microbiology Research Pub Date : 2023-07-10 DOI: 10.3390/microbiolres14030061

Amna Azam, Shahid Saleem Pall, I. Khan, Waqas Ahmad, T. Jamil, Aziz Ur Rehman, M. Imran, W. Shehzad, F. Melzer, M. F. Qamar, H. El-Adawy

{"title":"Evidence of Brucellosis in Hospitalized Patients of Selected Districts of Punjab, Pakistan","authors":"Amna Azam, Shahid Saleem Pall, I. Khan, Waqas Ahmad, T. Jamil, Aziz Ur Rehman, M. Imran, W. Shehzad, F. Melzer, M. F. Qamar, H. El-Adawy","doi":"10.3390/microbiolres14030061","DOIUrl":"https://doi.org/10.3390/microbiolres14030061","url":null,"abstract":"Brucellosis is a bacterial zoonosis worldwide and a potential health risk in Pakistan due to socio-economic reasons and lack of awareness, veterinary health and modern dairy-processing facilities. The aim of this study was to determine the burden of brucellosis in hospitalized patients of selected districts of Punjab, Pakistan. To this end, a total of 280 serum samples were collected from suspected patients in districts Lahore, Okara and Khushab between 2016 and 2018. Anti-Brucella antibodies were detected in 89 (31.8%) sera by Rose Bengal plate test (RBPT), and Brucella abortus DNA was amplified by polymerase chain reaction (PCR) in 42 (15%) samples. In univariable analysis, the seroprevalence varied significantly (p < 0.05) between districts with Lahore indicating 41/101 (40.6%) Odds ratio (OR) = 53.98 (7.22–403.67; 95% CI), Okara 47/99 (47.5%) OR = 71.4 (9.55–533.65; 95% CI) and Khushab 1/80 (1.3%) as reference. Similarly, exposure to aborted materials and a history of blood transfusion did associate significantly (p < 0.05), posing OR = 2.91 (1.58–5.36; 95% CI) and OR = 4.48 (2.56–7.84; 95% CI) risk. However, consumption of raw milk and history of blood transfusion did associate significantly (p < 0.05) in multivariable analysis indicating OR = 1.91 (1.09–3.34; 95% CI) and OR = 4.92 (2.76–8.75; 95% CI), respectively. The association of history of blood transfusion as a brucellosis risk factor underscores the need to create brucellosis awareness programs both at public and institutional levels in the medical and veterinary fields, as well as facilities and capacity building to delve deeper into the epidemiology of brucellosis in the country. Particular attention should be paid to chronically infected patients. Therefore, laboratory modernization, standardization and validation of the diagnostic tests, and staff trainings are required. Finally, pasteurization of the milk is recommended before consumption.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"168 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87945903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Fructooligosaccharides (FOS) on the Immune Response of the Shrimp Penaeus vannamei and on the Reduction in Vibrio spp. and Pseudomonas spp. in Cultures of Post-Larvae 低聚果糖(FOS)对凡纳滨对虾(Penaeus vannamei)免疫反应及培养后弧菌和假单胞菌减少的影响

IF 1.5

Microbiology Research Pub Date : 2023-07-08 DOI: 10.3390/microbiolres14030060

Yulaine Corrales Barrios, A. Roncarati, L. D. Martín Ríos, Maikelis Rodríguez González, Marbelys González Salotén, Yeidel López Zaldívar, A. Arenal

{"title":"Effects of Fructooligosaccharides (FOS) on the Immune Response of the Shrimp Penaeus vannamei and on the Reduction in Vibrio spp. and Pseudomonas spp. in Cultures of Post-Larvae","authors":"Yulaine Corrales Barrios, A. Roncarati, L. D. Martín Ríos, Maikelis Rodríguez González, Marbelys González Salotén, Yeidel López Zaldívar, A. Arenal","doi":"10.3390/microbiolres14030060","DOIUrl":"https://doi.org/10.3390/microbiolres14030060","url":null,"abstract":"Penaeus spp. are the most cultivated type of shrimp because they have rapid growth and good adaptation to farming conditions. Due to diseases that result in high mortality and a decreased product quality, the cultivation of these shrimp globally, and in Cuba in particular, comes with the risk of significant financial losses. This study examined the effect of fructooligosaccharide (FOS, 1-kestose) on the growth and immune response of shrimp, as well as the multitude of Vibrio spp. and Pseudomonas spp. in Penaeus vannamei post-larvae under culture conditions. Fructooligosaccharide was applied in a completely randomized manner at a concentration of 0.4%, in both experimental groups with seventeen tanks each. In the results of this investigation, animals of greater weight (control 6.8 ± 0.2 mg; FOS 9.5 ± 0.3 mg; p < 0.001), length (control 1.1 ± 0.1 mm; FOS 1.3 ± 0.1 mm; p < 0.001), and survival (control 61.7% (95% CI of median 54.2–70.0); FOS 76.6% (95% CI of median 72.1–84.2); p < 0.001) were obtained when administered FOS relative to the control. An increase in the activity of proteases (p < 0.001), enzymes of the innate immune system such as phenoloxidase (p < 0.001), and lysozymes (p < 0.001) was observed, as well as an increase in the number of lectins (p < 0.001). Changes in the microbiota could be observed, with a reduction in Vibrio spp. and Pseudomonas spp. (control 2.4 × 103 ± 0.5 × 103; FOS 1.1 × 102 ± 0.3 × 102; p < 0.001). FOS improves the quality of the post-larvae of P. vannamei as reflected in the length, weight, and survival of the animals. Moreover, FOS stimulates the P. vannamei immune system through the enzymatic activities of phenoloxidase, lysozyme, and a number of lectins. The reduction in the population load of Vibrio spp. and Pseudomonas spp. might be a consequence of the improvement in the quality and immune system of P. vannamei.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"37 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75856902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3L, Three-Lactobacilli on Recovering of Microbiome and Immune-Damage by Cyclophosphamide Chemotherapy—A Pilot Experiment in Rats— 3 -乳酸菌对环磷酰胺化疗大鼠微生物组和免疫损伤的恢复作用

IF 1.5

Microbiology Research Pub Date : 2023-07-06 DOI: 10.3390/microbiolres14030059

Shousong Yue, Zhenzhong Zhang, F. Bian, Yan Zhang, Gao Chen, Youfeng Zhu, Jun Li, J. Picimbon

引用次数: 0

Comparative Assessment of Antimicrobial Efficacy of Seven Surface Disinfectants against Eight Bacterial Strains in Saudi Arabia: An In Vitro Study 7种表面消毒剂对沙特阿拉伯8种细菌抗菌效果的比较研究

IF 1.5

Microbiology Research Pub Date : 2023-06-29 DOI: 10.3390/microbiolres14030058

Naif A. Jalal, Rozan A. Al-Atyyani, Hamdi M. Al-Said, Sami S. Ashgar, H. Faidah, A. Johargy, A. Momenah, A. Barhameen, S. Hariri, Farkad Bantun, Fadi S I Qashqari, E. B. Khidir, Mohammed H. Althagafi

{"title":"Comparative Assessment of Antimicrobial Efficacy of Seven Surface Disinfectants against Eight Bacterial Strains in Saudi Arabia: An In Vitro Study","authors":"Naif A. Jalal, Rozan A. Al-Atyyani, Hamdi M. Al-Said, Sami S. Ashgar, H. Faidah, A. Johargy, A. Momenah, A. Barhameen, S. Hariri, Farkad Bantun, Fadi S I Qashqari, E. B. Khidir, Mohammed H. Althagafi","doi":"10.3390/microbiolres14030058","DOIUrl":"https://doi.org/10.3390/microbiolres14030058","url":null,"abstract":"Environmental conditions in hospitals facilitate the growth and spread of pathogenic bacteria on surfaces such as floors, bed rails, air ventilation units, and mobile elements. These pathogens may be eliminated with proper disinfecting processes, including the use of appropriate surface disinfectants. In this study, we aimed to evaluate of the antibacterial effects of seven surface disinfectants (HAMAYA, DAC, AJAX, Jif, Mr. MUSCLE, CLOROX, and BACTIL) against eight bacterial strains Klebsiella pneumoniae, Enterobacter aerogenes, Acinetobacter baumannii, Serratia marcescens, Escherichia coli, vancomycin-resistant Enterococcus faecalis-ATCC 51299, methicillin-resistant Staphylococcus aureus-ATCC 43300, and Pseudomonas aeruginosa-ATCC 1544, using two methods. The first was to determine the effective contact time of disinfectant against the tested bacterial strains, and the second was an assessment of the disinfection efficacy of each disinfectant on six types of contaminated surfaces with on a mixture of the eight tested bacterial strains. The results showed the efficacy of the disinfectants against the tested strains depending on the effective contact time. BACTIL disinfectant showed an efficacy of 100% against all tested strains at the end of the first minute of contact time. HAMAYA, DAC, Jif, Mr. MUSCLE, and CLOROX showed 100% efficiency at the end of the fourth, fifth, sixth, seventh, and fourteenth minutes, respectively, while AJAX disinfectant required nineteen minutes of contact time to show 100% efficacy against all tested strains.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"11 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73637060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Antibody Persistence up to 6 Months after Additional Booster Vaccination with ChAdOx1 nCoV-19 Vaccine 加强接种ChAdOx1 nCoV-19疫苗后抗体持续时间长达6个月的比较

Microbiology Research Pub Date : 2023-06-16 DOI: 10.3390/microbiolres14020057

Pawita Suwanwattana, May Han, Tanawin Nopsopon, Phanupong Phutrakool, Chatpol Samuthpongtorn, Wannarat Pongpirul, Wisit Prasithsirikul, Krit Pongpirul

{"title":"Comparison of Antibody Persistence up to 6 Months after Additional Booster Vaccination with ChAdOx1 nCoV-19 Vaccine","authors":"Pawita Suwanwattana, May Han, Tanawin Nopsopon, Phanupong Phutrakool, Chatpol Samuthpongtorn, Wannarat Pongpirul, Wisit Prasithsirikul, Krit Pongpirul","doi":"10.3390/microbiolres14020057","DOIUrl":"https://doi.org/10.3390/microbiolres14020057","url":null,"abstract":"Vaccines are crucial for controlling the COVID-19 pandemic, and booster doses are becoming increasingly important. This study aimed to assess the efficacy of the ChAdOx1 nCoV-19 vaccine from AstraZeneca as a third dose in healthcare workers at different time intervals (one, three, and six months). Two methods to measure immune response—ELISA (EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany) and ELISpot (Mabtech AB, Macka Strand, Sweden)—were used. A total of 170 participants were included in the study. The results showed that while IgG levels decreased at six months compared to levels at one and three months, they were still significantly higher than the baseline. Furthermore, neutralizing levels at three and six months and after the third dose were not significantly different. These findings suggest that the immune response induced by the vaccine was robust and effective for several months. These results have significant implications for public health policymakers, as they provide strong support for booster vaccinations. The ChAdOx1 nCoV-19 vaccine appears to be a reliable option for preventing the spread of COVID-19, and this study provides valuable information for healthcare workers and policymakers in managing the pandemic.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135621262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Antibody Persistence up to 6 Months after Additional Booster Vaccination with ChAdOx1 nCoV-19 Vaccine 加强接种ChAdOx1 nCoV-19疫苗后抗体持续时间长达6个月的比较

IF 1.5

Microbiology Research Pub Date : 2023-06-16 DOI: 10.20944/preprints202304.0364.v1

Pawita Suwanwattana, M. Han, T. Nopsopon, P. Phutrakool, C. Samuthpongtorn, W. Pongpirul, W. Prasithsirikul, K. Pongpirul

{"title":"Comparison of Antibody Persistence up to 6 Months after Additional Booster Vaccination with ChAdOx1 nCoV-19 Vaccine","authors":"Pawita Suwanwattana, M. Han, T. Nopsopon, P. Phutrakool, C. Samuthpongtorn, W. Pongpirul, W. Prasithsirikul, K. Pongpirul","doi":"10.20944/preprints202304.0364.v1","DOIUrl":"https://doi.org/10.20944/preprints202304.0364.v1","url":null,"abstract":"Vaccines are crucial for controlling the COVID-19 pandemic, and booster doses are becoming increasingly important. This study aimed to assess the efficacy of the ChAdOx1 nCoV-19 vaccine from AstraZeneca as a third dose in healthcare workers at different time intervals (one, three, and six months). Two methods to measure immune response—ELISA (EUROIMMUN Medizinische Labordiagnostika AG, Luebeck, Germany) and ELISpot (Mabtech AB, Macka Strand, Sweden)—were used. A total of 170 participants were included in the study. The results showed that while IgG levels decreased at six months compared to levels at one and three months, they were still significantly higher than the baseline. Furthermore, neutralizing levels at three and six months and after the third dose were not significantly different. These findings suggest that the immune response induced by the vaccine was robust and effective for several months. These results have significant implications for public health policymakers, as they provide strong support for booster vaccinations. The ChAdOx1 nCoV-19 vaccine appears to be a reliable option for preventing the spread of COVID-19, and this study provides valuable information for healthcare workers and policymakers in managing the pandemic.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"33 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91332378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptococcus gattii Can Use the Cactus Pilosocereus spp. to Grow and Develop a Capsule and Produce Melanin In Vitro 加蒂隐球菌可以利用仙人掌生长、形成囊体并在体外产生黑色素

IF 1.5

Microbiology Research Pub Date : 2023-06-13 DOI: 10.3390/microbiolres14020056

Paola Ramos-Irizarry, Bárbara Sánchez, Y. Loperena-Álvarez

{"title":"Cryptococcus gattii Can Use the Cactus Pilosocereus spp. to Grow and Develop a Capsule and Produce Melanin In Vitro","authors":"Paola Ramos-Irizarry, Bárbara Sánchez, Y. Loperena-Álvarez","doi":"10.3390/microbiolres14020056","DOIUrl":"https://doi.org/10.3390/microbiolres14020056","url":null,"abstract":"Cryptococcus gattii is a pathogenic yeast, member of the C. neoformans/gattii complex. Previous work from our laboratory has established the presence of C. gattii on cacti lesions, providing proof that it can grow in a stressful environment. However, it is not known which part of the cactus the yeast uses for nutrients. The purpose of this research is to determine the ability of C. gattii to grow in different parts of the cactus to assess how the yeast adapts to grow in this unique environment. Cactus media were developed using the outer, inner, and whole cactus from Pilosocereus spp. Cryptcoccus gattii was grown on the different cactus media, along with potato dextrose agar as a control for 24 and 48 h at 30 °C. Compared to the control medium, yeast growth was reduced in all cactus media, while an increase in the capsule development of the yeast grown in the inner part and the whole-cactus media was observed. Interestingly, the yeast produces melanin when grown in the outer membrane medium, which was dependent on laccase, suggesting that the outer membrane may contain a precursor that stimulatates pigment production. To our knowledge, this is the first study addressing these key differences in the growth of C. gattii on different parts of the cactus.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"11 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91381981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potassium-Incorporated Titanium Oxide Nanoparticles Modulate Human Dendritic Cell Immune Response to Mycobacterium leprae 含钾氧化钛纳米颗粒调节人树突状细胞对麻风分枝杆菌的免疫反应

IF 1.5

Microbiology Research Pub Date : 2023-06-13 DOI: 10.3390/microbiolres14020055

Sam A. Warren, So Yoon Lee, J. Barragan, P. Kositangool, Hatsuko Yoshikubo, J. Cervantes

{"title":"Potassium-Incorporated Titanium Oxide Nanoparticles Modulate Human Dendritic Cell Immune Response to Mycobacterium leprae","authors":"Sam A. Warren, So Yoon Lee, J. Barragan, P. Kositangool, Hatsuko Yoshikubo, J. Cervantes","doi":"10.3390/microbiolres14020055","DOIUrl":"https://doi.org/10.3390/microbiolres14020055","url":null,"abstract":"The two polar clinical forms of leprosy, termed tuberculoid and lepromatous, have polarized cellular immune responses with complex immunological distinctions. The predominance of DCs in tuberculoid leprosy has been reported, while the lepromatous pattern of illness is associated with weak activation of local populations of DCs. TiO2 nanoparticles have previously been shown to induce maturation of these cells, leading to an inflammatory response similar to adjuvant usage in vaccine administration. We aimed to evaluate the effect of potassium-incorporated Ti oxide nanostructures, namely KTiOxs, in the response of human monocyte-derived DCs to live M. leprae. Human monocytic cell line dual THP-1, which harbors two inducible reporter plasmid systems for transcription factor activation of NF-κB and interferon regulating factor (IRF), was treated with titanium control or with 1 mol/L KOH-treated Ti or 10 mol/L KOH for 24 h. Subsequently, cells were infected with M. leprae. KTiOx nanoparticles increase DC phagocytic activity without inflammation. KTiOx exposure of DCs led to an increase in IRF activation with modulation of the inflammatory response to live M. leprae. It also led to differential secretion of the critical components of innate immune response and the development of cell-mediated immunity against intracellular pathogens. This study demonstrates the effect of nanostructures of KTiOxs and the usefulness of nanoparticle technology in the in vitro activation of human DCs against an infectious disease with a puzzling immune spectrum. Our findings may prompt future therapeutic strategies, such as DC immunotherapy for disseminated and progressive lepromatous lesions.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"29 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84308910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lichens from the Roosevelt River Area in the Brazilian Amazon 巴西亚马逊地区罗斯福河地区的地衣

IF 1.5

Microbiology Research Pub Date : 2023-06-08 DOI: 10.3390/microbiolres14020054

A. Aptroot

引用次数: 0

Relevance of the Microbiota on Ocular Diseases: A Bibliometric and Citation Network Analysis 微生物群与眼部疾病的相关性:文献计量学和引文网络分析

IF 1.5

Microbiology Research Pub Date : 2023-06-06 DOI: 10.3390/microbiolres14020053

M. A. Sanchez-Tena, B. Galvez, C. Martínez-Pérez, C. Alvarez-Peregrina

{"title":"Relevance of the Microbiota on Ocular Diseases: A Bibliometric and Citation Network Analysis","authors":"M. A. Sanchez-Tena, B. Galvez, C. Martínez-Pérez, C. Alvarez-Peregrina","doi":"10.3390/microbiolres14020053","DOIUrl":"https://doi.org/10.3390/microbiolres14020053","url":null,"abstract":"Background: This study aimed to analyze the relevance of different publications about microbiota on ocular diseases and their authors through a citation network analysis. In addition, the different research areas and the most cited publications have been identified. Methods: The bibliographic search was carried out through the Web of Science (WOS) database, using the following search term: “microbiota AND (vision OR eye OR visual)” for the period between 1995 and December 2022. The Citation Network Explorer and the CiteSpace software have been used to analyze the different publications. Results: 705 publications were found in the field of microbiota on ocular diseases, together with 1014 citation networks. The year 2022 was the year with more publications. The first authors with the highest number of publications in the microbiota on the ocular surface field were Chisari G, Chisari CG, and Li Y. This field is multidisciplinary, highlighting “microbiology” and “ophthalmology” as the main research areas. Publications were clustered into three main groups allowing the identification of the main research topics in this field. The principal was the composition and diversity of the bacterial community on the ocular surface of patients with several pathologies. Conclusion: It could be useful for researchers to choose suitable collaborators or projects to promote their research on the role of microbiota on ocular diseases, as well as to know the main research topics that are of major interest today.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"27 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85332390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0