Microbiology Research最新文献_第10页

The Influence of Pre-Existing Immunity against Human Common Cold Coronaviruses on COVID-19 Susceptibility and Severity 已有的人类普通感冒冠状病毒免疫对COVID-19易感性和严重程度的影响

Microbiology Research Pub Date : 2023-09-14 DOI: 10.3390/microbiolres14030093

Erick De La Torre Tarazona, Daniel Jiménez, Daniel Marcos-Mencía, Alejandro Mendieta-Baro, Alejandro Rivera-Delgado, Beatriz Romero-Hernández, Alfonso Muriel, Mario Rodríguez-Domínguez, Sergio Serrano-Villar, Santiago Moreno

{"title":"The Influence of Pre-Existing Immunity against Human Common Cold Coronaviruses on COVID-19 Susceptibility and Severity","authors":"Erick De La Torre Tarazona, Daniel Jiménez, Daniel Marcos-Mencía, Alejandro Mendieta-Baro, Alejandro Rivera-Delgado, Beatriz Romero-Hernández, Alfonso Muriel, Mario Rodríguez-Domínguez, Sergio Serrano-Villar, Santiago Moreno","doi":"10.3390/microbiolres14030093","DOIUrl":"https://doi.org/10.3390/microbiolres14030093","url":null,"abstract":"The susceptibility to SARS-CoV-2 infection and the severity of COVID-19 manifestations vary significantly among individuals, prompting the need for a deeper understanding of the disease. Our objective in this study was to investigate whether previous infections with human common cold coronaviruses (hCCCoV) might impact susceptibility to and the progression of SARS-CoV-2 infections. We assessed the serum antibody levels against SARS-CoV-2 and four hCCCoV (H-CoV-OC43, -NL63, -HKU1, and -229E) in three distinct populations: 95 uninfected individuals (COVID-19-negative), 83 individuals with mild or asymptomatic COVID-19 (COVID-19-mild), and 45 patients who died due to COVID-19 (COVID-19-severe). The first two groups were matched in terms of their exposure to SARS-CoV-2. We did not observe any differences in the mean antibody levels between the COVID-19-mild and the COVID-19-negative participants. However, individuals in the COVID-19-mild group exhibited a higher frequency of antibody levels (sample/control) > 0.5 against H-CoV-HKU1, and >1 against H-CoV-229E and -OC43 (p < 0.05). In terms of severity, we noted significantly elevated H-CoV-NL63 IgG levels in the COVID-19-severe group compared to the other groups (p < 0.01). Our findings suggest a potential mild influence of hCCCoV antibody levels on the susceptibility to SARS-CoV-2 infection and the severity of COVID-19. These observations could aid in the development of strategies for predicting and mitigating the severity of COVID-19.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134913683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Triterpenoid High-Performance Liquid Chromatography Analytical Profiles of the Mycelia of Ganoderma lucidum (lingzhi) 灵芝菌丝体三萜高效液相色谱分析谱

Microbiology Research Pub Date : 2023-09-14 DOI: 10.3390/microbiolres14030092

Deng-Hai Chen, Jian-Yuan Wang, Mon-Tarng Chen, Yen-Chun Liu, Kuang-Dee Chen

{"title":"The Triterpenoid High-Performance Liquid Chromatography Analytical Profiles of the Mycelia of Ganoderma lucidum (lingzhi)","authors":"Deng-Hai Chen, Jian-Yuan Wang, Mon-Tarng Chen, Yen-Chun Liu, Kuang-Dee Chen","doi":"10.3390/microbiolres14030092","DOIUrl":"https://doi.org/10.3390/microbiolres14030092","url":null,"abstract":"(1) Background: Ganoderic acids (GAs) are specific triterpenes of Ganoderma lucidum. The HPLC fingerprint profile of GAs of the fruiting body is well known, but their mycelial fingerprinting remains unclear. (2) Methods: An ethanol extract of the mycelium of G. lucidum (YK-01) was further purified via preparative HPLC. The triterpenoid compositions for four strains of G. lucidum and one strain of G. formosanum (purple lingzhi) were analyzed using HPLC. (3) Results: Nineteen lanostane triterpenes, including five new triterpenes, GA-TP (1), ganodermic acid Jc (GmA-Jc) (2), GmA-Jd (3), GA-TQ1 (4), and ganoderal B1 (5), and fourteen known triterpenes 6–19 were isolated from the ethanol extract. Their structures were identified by mass and extensive NMR spectroscopy. A green chemical HPLC analytical method was developed using ethanol and acetic acid as a mobile phase, and all isolated compounds can be well separated. These triterpenes comprise a unique HPLC chromatograph of the G. lucidum mycelium. All four G. lucidum strains showed the same HPLC chromatographic pattern, whereas G. formosanum displayed a different pattern. Quantitation methods for ganoderic acid T (10) and S (12) were also validated. (4) Conclusions: The triterpenoid HPLC analytical method can be used to identify the G. lucidum species and to determine the contents of GA-T and GA-S.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134913681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Epidemiology of Porcine Circovirus Type 2 and Porcine Parvoviruses in Guangxi Autonomous Region, China 广西猪圆环病毒2型和猪细小病毒的分子流行病学研究

Microbiology Research Pub Date : 2023-09-13 DOI: 10.3390/microbiolres14030090

Pin Chen, Geng Wang, Jiping Chen, Weichao Zhang, Yin He, Ping Qian

{"title":"Molecular Epidemiology of Porcine Circovirus Type 2 and Porcine Parvoviruses in Guangxi Autonomous Region, China","authors":"Pin Chen, Geng Wang, Jiping Chen, Weichao Zhang, Yin He, Ping Qian","doi":"10.3390/microbiolres14030090","DOIUrl":"https://doi.org/10.3390/microbiolres14030090","url":null,"abstract":"Both porcine circovirus (PCV) and porcine parvovirus (PPV) cause various diseases and bring huge economic losses to the global swine industry. PCV2 is associated with several diseases and syndromes, including postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS) and porcine respiratory disease complex (PRDC). The classical PPV is one of the most common causes of reproductive failure in pigs. In this study, tissue samples (tonsil, lung, mesenteric lymph node, hilar lymph node and superficial inguinal lymph node) were collected from pigs with suspected PCV2-associated disease (PCVAD), and viral DNA was extracted. The coinfection of PCV2 and PPV1–5 was detected using the polymerase chain reaction (PCR) method. Phylogenetic analysis based on capsid genes of PCV2, PPV2, PPV3 and PPV5 was conducted. The prevalence rates of PCV2, PPV1, PPV2, PPV3, PPV4 and PPV5 were 51.2%, 15.9%, 36.6%, 19.5%, 14.6% and 10.9% on the individual pig level, respectively. The coinfection rates of PCV2 with PPV1, PPV2, PPV3, PPV4 and PPV5 were 8.5%, 25.6%, 17.1%, 13.4% and 3.7%, respectively. The prevalence of PPV2, PPV3 and PPV4 in PCV2-positive pigs was significantly higher than those in PCV2-negative pigs. Phylogenetic analyses were performed using the neighbor-joining (NJ) method with 1000 bootstraps. The results indicated the existence of PCV2d and two major clusters of PPV2, PPV3 and PPV5 in the Guangxi Autonomous Region. PCV2d was the dominant strain, and the novel PPVs were circulating in domestic pigs in the Guangxi Autonomous Region. The results of this study underline the importance of active surveillance of PCV2d and PPVs from the swine population in this area.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eradication of Bovine Mastitis Caused by the Pathogenic Microalga Prototheca bovis on a Dairy Cattle Farm: A Case Report 某奶牛场原芽微藻致牛乳腺炎根除1例

Microbiology Research Pub Date : 2023-09-13 DOI: 10.3390/microbiolres14030091

Monika Beinhauerova, Monika Moravkova, Ruzena Seydlova, Magdalena Crhanova

{"title":"Eradication of Bovine Mastitis Caused by the Pathogenic Microalga Prototheca bovis on a Dairy Cattle Farm: A Case Report","authors":"Monika Beinhauerova, Monika Moravkova, Ruzena Seydlova, Magdalena Crhanova","doi":"10.3390/microbiolres14030091","DOIUrl":"https://doi.org/10.3390/microbiolres14030091","url":null,"abstract":"The achlorophyllous unicellular microalga of the genus Prototheca, a causative agent of bovine mammary gland infection, is receiving increasing attention in the field of veterinary medicine. Mastitis caused by these algae leads to significant economic losses for farmers worldwide and represents a source of threat to raw milk quality and dairy food-chain safety. This case report describes an outbreak of bovine mastitis in dairy cattle caused by Prototheca bovis and the on-farm practices that resulted in the recovery of the affected herd and elimination of the presence of Prototheca spp. in the farm environment. Effective management strategies that played a crucial role in protothecosis eradication included regular screening, timely identification, strict separation of Prototheca-positive cows, a change of housing regime associated with the utilisation of straw mattresses and removal of deep bedding and, finally, the introduction of intermediate disinfection of teat cups with peracetic acid to prevent the spread of infection to other healthy individuals. The eradication process lasted approximately three years and required the removal of 139 cows from the farm. The corrective and preventive measures described in this case report provide guidance to farmers on how to successfully deal with protothecal mastitis on farms.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135782468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aureimonas altamirensis: First Isolation from a Chicken Slaughterhouse in Italy Followed by Genotype and Phenotype Evaluations altamirenis金黄色单胞菌:首次从意大利鸡屠宰场分离并进行基因型和表型评价

Microbiology Research Pub Date : 2023-09-12 DOI: 10.3390/microbiolres14030089

Davide Buzzanca, Elisabetta Chiarini, Ilaria Mania, Francesco Chiesa, Valentina Alessandria

{"title":"Aureimonas altamirensis: First Isolation from a Chicken Slaughterhouse in Italy Followed by Genotype and Phenotype Evaluations","authors":"Davide Buzzanca, Elisabetta Chiarini, Ilaria Mania, Francesco Chiesa, Valentina Alessandria","doi":"10.3390/microbiolres14030089","DOIUrl":"https://doi.org/10.3390/microbiolres14030089","url":null,"abstract":"The presence of foodborne pathogens in meat is linked to several contamination sources, and the slaughterhouse environment represents a relevant reservoir of contamination. Aureimonas altamirensis is a Gram-negative bacteria associated with different isolation sources, including human clinical cases. This study aims to identify and characterize an A. altamirensis isolate from chicken guts collected in an Italian slaughterhouse. The study approach includes whole-genome analysis jointly with phenotypical tests. Whole-genome sequencing (WGS) confirms the initial MALDI-TOF MS identification, finding putative virulence and biofilm-related genes. Moreover, the gene class evaluation reveals that the numerically largest gene category in the A. altamirensis genome is related to amino acid metabolism and transport. The analyses performed on a human gut mucus-producing cell line (HT29-MTX-E12) demonstrated the ability of A. altamirensis to colonize the host cell layer. Moreover, the antibiotic resistance test showed a high resistance of A. altamirensis to gentamicin (MIC 0.5 mg/L). The detection of a potential pathogenic and antibiotic-resistant A. altamirensis strain isolated from a slaughterhouse underlines the necessity of active surveillance studies focused on this species and the need for further studies about A. altamirensis in foods.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135885594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichoderma Species Problematic to the Commercial Production of Pleurotus in Italy: Characterization, Identification, and Methods of Control 意大利平菇商业化生产中存在问题的木霉种类:特征、鉴定和控制方法

Microbiology Research Pub Date : 2023-09-12 DOI: 10.3390/microbiolres14030088

Nadia Lombardi, Angela Pironti, Gelsomina Manganiello, Roberta Marra, Francesco Vinale, Stefania Vitale, Matteo Lorito, Sheridan Lois Woo

{"title":"Trichoderma Species Problematic to the Commercial Production of Pleurotus in Italy: Characterization, Identification, and Methods of Control","authors":"Nadia Lombardi, Angela Pironti, Gelsomina Manganiello, Roberta Marra, Francesco Vinale, Stefania Vitale, Matteo Lorito, Sheridan Lois Woo","doi":"10.3390/microbiolres14030088","DOIUrl":"https://doi.org/10.3390/microbiolres14030088","url":null,"abstract":"Nine isolates of Trichoderma were obtained from the diverse phases of compost preparation and the production of different commercial varieties of oyster mushrooms Pleurotus spp. with the apparent presence of green mould disease. The isolates were morphologically and genetically characterized. Molecular fingerprinting indicated that the isolates belonged to the species T. pleuroticola and T. harzianum. In order to identify control measures, changes in temperature, pH, and application of fungicides were tested on the present isolates, using known Trichoderma biocontrol strains as a reference. Fungicide effects on the growth of Pleurotus isolates were also assessed. The optimal growth temperatures were 25 °C for Trichoderma isolates and 28 °C for Pleurotus isolates, and Trichoderma always grew faster than Pleurotus. In particular, a reduction of about 30% was recorded for some of the Trichoderma isolates when comparing the colony growth at 25 and 28 °C. Trichoderma isolates developed well within a wide pH range, with the best growth occurring between pH 5 and 7, whereas Pleurotus preferred more alkaline conditions (pH 8 to 9). Prochloraz and metrafenone were found to inhibit Trichoderma growth with different dose responses that did not affect the growth of Pleurotus spp. In particular, metrafenone was the most effective active ingredient that inhibited the majority of the Trichoderma isolates (1–25% growth reduction).","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135885467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Potency and Harnessing the Antibacterial Activities of Plant Oils against Foodborne Pathogens 揭示植物油对食源性病原菌的抑菌活性并加以利用

Microbiology Research Pub Date : 2023-09-09 DOI: 10.3390/microbiolres14030087

Vijaya Samoondeeswari Selvarajan, Ramganesh Selvarajan, Jeevan Pandiyan, Akebe Luther King Abia

{"title":"Unveiling the Potency and Harnessing the Antibacterial Activities of Plant Oils against Foodborne Pathogens","authors":"Vijaya Samoondeeswari Selvarajan, Ramganesh Selvarajan, Jeevan Pandiyan, Akebe Luther King Abia","doi":"10.3390/microbiolres14030087","DOIUrl":"https://doi.org/10.3390/microbiolres14030087","url":null,"abstract":"The rising concerns regarding antibiotic resistance and the harmful effects of synthetic preservatives have led to an increasing interest in exploring natural alternatives. Plant oils have been traditionally used for their antimicrobial properties, but systematic investigations into their efficacy against foodborne pathogens are necessary for potential applications in food preservation. This study aimed to evaluate the antibacterial potential of various plant oils (neem, coconut, castor, and olive oil) against common foodborne pathogens and analyze their chemical composition using gas chromatography–mass spectrometry (GC-MS). The oils were tested against foodborne pathogens using the disk diffusion method. Minimum inhibitory concentrations (MICs) were determined to assess the potency of the oils. GC-MS was employed to identify the compounds present in each oil. Neem oil exhibited significant antibacterial activity against all tested pathogens, with pronounced effects against Staphylococcus aureus and Bacillus cereus. Coconut oil showed notable activity against Listeria monocytogenes. Castor oil displayed moderate activity, while olive oil exhibited minimal antibacterial effects. The GC-MS analysis revealed a diverse array of compounds in neem oil, which is likely to contribute to its potent antibacterial properties. Neem and coconut oils, owing to their rich bioactive components, emerged as promising candidates for the development of natural antimicrobial agents. These brief findings support the potential application of plant oils in food preservation and emphasize the need for further research into understanding the underlying mechanisms and optimizing their use.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136192012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Molecular Epidemiology of Clostridioides difficile Infection in Central India: A Prospective Observational Cohort Study 印度中部艰难梭菌感染的分子流行病学:一项前瞻性观察队列研究

Microbiology Research Pub Date : 2023-09-08 DOI: 10.3390/microbiolres14030086

Rima Biswas, Nick Pinkham, Seth T. Walk, Qian Wang, Shrikant Ambalkar, Ashish R. Satav, Mark H. Wilcox, Rahul Reghunath, Kiran Chawla, Padmaja A. Shenoy, Amit R. Nayak, Aliabbas A. Husain, Dhananjay V. Raje, Rajpal Singh Kashyap, Tanya M. Monaghan

{"title":"The Molecular Epidemiology of Clostridioides difficile Infection in Central India: A Prospective Observational Cohort Study","authors":"Rima Biswas, Nick Pinkham, Seth T. Walk, Qian Wang, Shrikant Ambalkar, Ashish R. Satav, Mark H. Wilcox, Rahul Reghunath, Kiran Chawla, Padmaja A. Shenoy, Amit R. Nayak, Aliabbas A. Husain, Dhananjay V. Raje, Rajpal Singh Kashyap, Tanya M. Monaghan","doi":"10.3390/microbiolres14030086","DOIUrl":"https://doi.org/10.3390/microbiolres14030086","url":null,"abstract":"This prospective observational cohort study aimed to establish and compare baseline rates of Clostridioides difficile infection (CDI) in community and hospitalized patients in Nagpur and rural Melghat Maharashtra, including adults aged ≥18 years with a diagnosis of diarrhoea as defined as 3 or more loose stools in a 24 h period. All diarrhoeal samples were tested for CDI using the C. diff Quik Chek Complete enzyme immunoassay. C. difficile-positive stool samples were characterised by toxigenic culture, antimicrobial susceptibility testing and PCR ribotyping. C. difficile testing was performed on 1683 patients with acute diarrhoea. A total of 54 patients (3.21%; 95% CI: 2.42–4.17) tested positive for both the GDH antigen and free toxin. The risk factors for CDI included the presence of co-morbidities, antibiotic usage, and immunosuppression. The detected PCR ribotypes included 053-16, 017, 313, 001, 107, and 216. Our findings show that toxigenic C. difficile is an important but neglected aetiologic agent of infective diarrhoea in Central India. These results underscore the need to enhance the awareness and testing of patients with diarrhoea in India regarding the presence of toxigenic C. difficile, particularly in high-risk individuals with multiple co-morbidities, immunosuppression, and recent or ongoing antibiotic exposure or hospitalization.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136362378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Compounds from Balanites aegyptiaca against Squalene Epoxidase of Micropsorum gypseum—In Vitro and In Silico Studies 埃及巴兰类化合物对石膏微孢菌角鲨烯环氧化酶的体外和硅质研究

IF 1.5

Microbiology Research Pub Date : 2023-09-04 DOI: 10.3390/microbiolres14030085

Mohamed Hussain Syed Abuthakir, V. Hemamalini, Reham M. Alahmadi, Anis Ahamed, A. A. Hatamleh, Razack Abdullah, Jeyam Muthusamy

{"title":"Evaluation of Compounds from Balanites aegyptiaca against Squalene Epoxidase of Micropsorum gypseum—In Vitro and In Silico Studies","authors":"Mohamed Hussain Syed Abuthakir, V. Hemamalini, Reham M. Alahmadi, Anis Ahamed, A. A. Hatamleh, Razack Abdullah, Jeyam Muthusamy","doi":"10.3390/microbiolres14030085","DOIUrl":"https://doi.org/10.3390/microbiolres14030085","url":null,"abstract":"Microsporum gypseum is a dermatophyte with a geophilic nature that is found all over the globe. It mainly causes tinea in the scalp, arms, and legs in humans. Squalene epoxidase (SE) is a crucial enzyme in M. gypseum for the biosynthesis of ergosterol. The medicinal plant Balanites aegyptiaca is an abundant supply of secondary constituents with great therapeutic values. In this research, the fruit epicarp portion was used to inhibit M. gypseum using experimental and computational techniques. The anti-dermatophytic activity of epicarp extracts on M. gypseum was evaluated using the poison plate method at five different concentrations. At 3 mg/mL, the M. gypseum was completely controlled by the fractioned chloroform extract of epicarp. The compounds from previous research were utilized for docking studies (Abuthakir et al., 2022). The ideal compounds and the drug terbinafine were then docked using Schrödinger’s Glide module. It demonstrates that (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside outperforms other substances and the drug terbinafine in docking analysis. Desmond, Schrödinger Molecular Dynamics simulations were also performed for (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside-squalene epoxidase complexes. The complex appears to be more stable, according to the MD simulation research. This study indicates that (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside could be used as a potential inhibitor of M. gypseum growth, and it could be studied further.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86766247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular Mycorrhizal Fungus Funneliformis mosseae Improves Soybean Growth Even in Soils with Good Nutrition 丛枝菌根真菌即使在营养良好的土壤中也能促进大豆的生长

IF 1.5

Microbiology Research Pub Date : 2023-08-31 DOI: 10.3390/microbiolres14030084

V. Řezáčová, Ema Némethová, I. Stehlíková, A. Czakó, M. Gryndler

{"title":"Arbuscular Mycorrhizal Fungus Funneliformis mosseae Improves Soybean Growth Even in Soils with Good Nutrition","authors":"V. Řezáčová, Ema Némethová, I. Stehlíková, A. Czakó, M. Gryndler","doi":"10.3390/microbiolres14030084","DOIUrl":"https://doi.org/10.3390/microbiolres14030084","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) improve plant growth and may be useful in maintaining and even restoring soil. However, data on the latter function are sparse and only indirect, which is especially true for conventional management conditions with adequate nutrient availability. Our study focused on utilizing the prevalent AMF species, Funneliformis mosseae, to enhance Glycine max production, while also exploring its partly explored impact on soil aggregation. Working in greenhouse conditions, we examined whether, in a nutrient-sufficient environment, AMF would improve crop biomass accumulation and nutrition, as well as the stability of soil aggregates (SAS). We also looked for a synergistic effect of dual inoculation using AMF and symbiotic rhizobium. Plants were or were not inoculated with AMF or Bradyrhizobium japonicum in a two-factorial design. AMF inoculation increased soybean biomass, but AMF inoculation had no impact on P and N input to the shoots. Mycorrhiza did not affect either glomalin abundance or SAS. All the impacts were, however, independent from rhizobial inoculation, which was ineffective in this nutrient-available environment. Our assay suggests that arbuscular mycorrhiza may have a positive effect on soybean growth even under conventional management with adequate nutrition. The positive effects of AMF on soybean growth, together with the fact that AMF generally do not thrive in good nutrient availability, should be taken into account when planning mineral fertilization levels.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78943224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0