Microbiology Research最新文献_第10页

Antiviral Potential of Antillogorgia americana and elisabethae Natural Products against nsp16–nsp10 Complex, nsp13, and nsp14 Proteins of SARS-CoV-2: An In Silico Investigation 抗美洲苦参和白绿科天然产物对SARS-CoV-2病毒nsp16-nsp10复合体、nsp13和nsp14蛋白的抗病毒潜力:一项计算机研究

IF 1.5

Microbiology Research Pub Date : 2023-07-28 DOI: 10.3390/microbiolres14030068

Omkar Pokharkar, Hari G Lakshmanan, G. Zyryanov, M. Tsurkan

{"title":"Antiviral Potential of Antillogorgia americana and elisabethae Natural Products against nsp16–nsp10 Complex, nsp13, and nsp14 Proteins of SARS-CoV-2: An In Silico Investigation","authors":"Omkar Pokharkar, Hari G Lakshmanan, G. Zyryanov, M. Tsurkan","doi":"10.3390/microbiolres14030068","DOIUrl":"https://doi.org/10.3390/microbiolres14030068","url":null,"abstract":"Biomolecules of marine origin have many applications in the field of biotechnology and medicine, but still hold great potential as bioactive substances against different diseases. The purification or total synthesis of marine metabolites is expensive, and requires a reliable selection method to reveal their pharmaceutical potential prior to clinical validation. This study aimed to explore the hidden potential of natural products from the gorgonian genus Antillogorgia as anti-SARS-CoV-2 agents, via binding affinity assessments and molecular dynamics (MDs) simulations. The three-dimensional protein structures of the nsp16–nsp10 complex, nsp13, and nsp14 were acquired from the RCSB PDB database. All 165 natural products (NPs) were discovered using the PubChem, ChemSpider, and CMNPD databases. The freeware Autodock Vina was used to conduct the molecular docking procedure, once the proteins and ligands were prepared using BIOVIA discovery studio and Avogadro software v1.95. Before running MDs simulations using the CABS-flex 2.0 website, the binding affinity assessments and amino acid interactions were carefully examined. Just twelve NPs were selected, and five of those NPs interacted optimally with the catalytic amino acids of proteins. To conclude, pseudopterosin A (−8.0 kcal/mol), seco-pseudopterosin A (−7.2 kcal/mol), sandresolide B (−6.2 kcal/mol), elisabatin A (−7.0 kcal/mol), and elisapterosin A (−10.7 kcal/mol) appeared to be the most promising candidates against the nsp16–nsp10, nsp13, and nsp14 proteins.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"48 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82532089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Transcriptome Analysis Deciphers Trichoderma koningiopsis C5-9 Strategies against Plant Pathogen Botrytis cinerea 转录组分析破解柯宁木霉C5-9对抗植物病原菌灰霉病的策略

IF 1.5

Microbiology Research Pub Date : 2023-07-27 DOI: 10.3390/microbiolres14030067

Min Yuan, Chunliu Zuo, Wenda Xu, Li Zhang, Xinyue Guo, Xinyue Yan, Song-Wei Li, Yanling Li, Lan Zhang, Jiaqi Geng, Ya-Lun Huang

{"title":"Transcriptome Analysis Deciphers Trichoderma koningiopsis C5-9 Strategies against Plant Pathogen Botrytis cinerea","authors":"Min Yuan, Chunliu Zuo, Wenda Xu, Li Zhang, Xinyue Guo, Xinyue Yan, Song-Wei Li, Yanling Li, Lan Zhang, Jiaqi Geng, Ya-Lun Huang","doi":"10.3390/microbiolres14030067","DOIUrl":"https://doi.org/10.3390/microbiolres14030067","url":null,"abstract":"The plant pathogen Botrytis cinerea (B. cinerea) causes severe plant diseases worldwide. Trichoderma is widely used as a biocontrol agent against B. cinerea through multiple biocontrol mechanisms. However, Trichoderma spp. with high biocontrol efficiency against B. cinerea under low-temperature conditions are barely reported. This study aimed to find potential low-temperature resistance biocontrol Trichoderma spp. against B. cinerea, and to characterize the biological principles underlying the activity of Trichoderma. Trichoderma koningiopsis (T. koningiopsis) C5-9 could fully overgrow a B. cinerea colony at 16 °C in a dual-culture assay. Treatment of cucumber leaves with T. koningiopsis C5-9 fermentation broth using the dipping method prior to B. cinerea inoculation significantly reduced the necrotic lesion diameter, with an inhibition rate of 55.30%. T. koningiopsis C5-9 could be successfully cultivated using the mycelia of B. cinerea as a carbon source at 16 °C. Transcriptomic analysis indicated that the origin recognition complex, organic substance catabolic process, and peroxisome were involved in the responses of T. koningiopsis C5-9 to B. cinerea. The findings of this study not only identified T. koningiopsis C5-9 as a potential biological control agent inhibiting B. cinerea under low-temperature conditions, but also provided new insights to develop a deeper understanding of the activity of Trichoderma against B. cinerea for plant protection.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"53 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90765983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bioprospecting of a Thermostable L-Methioninase from Alcaligenes aquatilis BJ-1 in Agro-Industrial Waste 从农业工业废弃物中提取的耐热型l -蛋氨酸酶的生物勘探

IF 1.5

Microbiology Research Pub Date : 2023-07-27 DOI: 10.3390/microbiolres14030066

Bhumi Javia, Megha S. Gadhvi, S. Vyas, P. Dudhagara, D. Shyu, Yih-Yuan Chen, Dushyant Dudhagara

{"title":"Bioprospecting of a Thermostable L-Methioninase from Alcaligenes aquatilis BJ-1 in Agro-Industrial Waste","authors":"Bhumi Javia, Megha S. Gadhvi, S. Vyas, P. Dudhagara, D. Shyu, Yih-Yuan Chen, Dushyant Dudhagara","doi":"10.3390/microbiolres14030066","DOIUrl":"https://doi.org/10.3390/microbiolres14030066","url":null,"abstract":"L-methioninase is an enzyme that has recently gained significant interest in the scientific community because of its potential as a targeted therapy for cancer. This study aims to isolate and identify extremophilic bacteria that could produce L-methioninase and to access the enzymatic potential of isolated bacteria under stress conditions, specifically in agro-industrial waste. In this study, a rare marine bacterium, Alcaligenes aquatilis BJ-1, exhibited the highest specific activity of 4.61 U/mg at an optimum pH of 8.3. The L-methioninase was purified 4.3-fold and 7.15-fold by acetone precipitation and Sephadex G-100 gel filtration chromatography, which revealed a molecular weight of 46 kDa. In addition, agriculture waste materials such as cottonseed oil cake had the highest L-methioninase production. Moreover, A. aquatilis BJ-1 can tolerate and produce enzymes in the presence of 10% NaCl, 6% KCl, and 4% MgSO4. Similarly, substrates such as L-asparagine, L-glutamine, L-alanine, and L-tyrosine were found suitable to increase enzyme production. The strain produced L-methioninase in the presence of various heavy metals. Maximum enzyme activity was found in Zn2+ at 0.1% (2.52 U/mL), Li2+ at 0.03% (2.90 U/mL), and Ni2+ at 0.01% (2.78 U/mL), as compared to the control (2.23 U/mL) without metal. Enzyme production was also observed at a high temperature (60 °C), with the produced enzymes possessing antioxidant properties. In addition, no hemolytic activity was observed. The results indicate that A. aquatilis BJ-1 is an appropriate bacterium for metal bioremediation procedures in unfavorable circumstances.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"58 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77787238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microbiome of Clothing Items Worn for a Single Day in a Non-Healthcare Setting 在非医疗环境中穿一天衣服的微生物群

IF 1.5

Microbiology Research Pub Date : 2023-07-26 DOI: 10.3390/microbiolres14030065

K. Whitehead, Jake Eppinger, V. Srinivasan, M. K. Ijaz, R. Nims, J. McKinney

{"title":"Microbiome of Clothing Items Worn for a Single Day in a Non-Healthcare Setting","authors":"K. Whitehead, Jake Eppinger, V. Srinivasan, M. K. Ijaz, R. Nims, J. McKinney","doi":"10.3390/microbiolres14030065","DOIUrl":"https://doi.org/10.3390/microbiolres14030065","url":null,"abstract":"When worn, clothing acquires a microbiome of bacteria and fungi derived from the wearer’s skin and from the environment. The types of bacteria and fungi that may be recovered from clothing in healthcare settings have been well characterized, but less is known regarding the microbiome of clothing worn in non-healthcare settings and the possible roles that such clothing may play in microbial exchange. Culture-based methods and culture-independent genomic sequencing were used to enumerate and identify bacteria and fungi recovered from T-shirts, baby onesies, socks, and underwear worn for a single day after having been purchased new, washed, and dried. The highest bacterial loads were recovered from socks, underwear, and onesies (>106 colony-forming units [cfu]/sample) and the highest fungal loads were obtained from socks and underwear (>5 × 102 cfu/sample). The sequencing method identified opportunistic pathogens present in the samples, including members of genus Staphylococcus and Corynebacterium, as well as anaerobic members of the family Clostridiales. The opportunistic fungal pathogen Candida parapsilosis was identified in a high proportion of worn clothing samples. These results suggest that clothing may represent a pathogen reservoir and a vector for microbial exchange between household occupants or the community outside of the home.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"10 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84161880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microorganisms and Climate Change: A Not so Invisible Effect 微生物与气候变化:一个并非无形的影响

IF 1.5

Microbiology Research Pub Date : 2023-07-25 DOI: 10.3390/microbiolres14030064

A. Ibáñez, Sonia Garrido-Chamorro, C. Barreiro

{"title":"Microorganisms and Climate Change: A Not so Invisible Effect","authors":"A. Ibáñez, Sonia Garrido-Chamorro, C. Barreiro","doi":"10.3390/microbiolres14030064","DOIUrl":"https://doi.org/10.3390/microbiolres14030064","url":null,"abstract":"The effect of climate change on flora and fauna has been widely discussed for years. However, its consequences on microorganisms are generally poorly considered. The main effect of climate change on microbiota is related to biodiversity changes in different regions of the planet, mainly due to variations in temperature. These alterations are resulting in a worldwide (re)distribution of pathogens, which was not considered a few years ago. They mainly affect different food chain sectors (such as agriculture, livestock and fishing), as well as human health. Hence, the spread of numerous animal and plant pathogens has been observed in recent years from south to north (especially in America, Europe and Asia), leading to the spread of numerous plant and animal diseases, which results in economic and ecological losses. In addition, global warming that accompanies climate change could also be related to emerging antibiotic resistance. However, the mitigation of climate change goes hand in hand with microorganisms, which can help us through different natural and industrial processes. Thus, this manuscript presents the direct and indirect effects of climate change on microorganisms described up to date and how they act on this worldwide phenomenon.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"41 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90342006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Molecular Epidemiology of Enterovirus in a Birth Cohort in Nepal 尼泊尔出生队列肠病毒的分子流行病学研究

IF 1.5

Microbiology Research Pub Date : 2023-07-20 DOI: 10.3390/microbiolres14030063

S. Shrestha, J. Shrestha, T. Strand, S. Numanovic, A. Andreassen, J. Dembinski, R. Vikse, S. Dudman

{"title":"The Molecular Epidemiology of Enterovirus in a Birth Cohort in Nepal","authors":"S. Shrestha, J. Shrestha, T. Strand, S. Numanovic, A. Andreassen, J. Dembinski, R. Vikse, S. Dudman","doi":"10.3390/microbiolres14030063","DOIUrl":"https://doi.org/10.3390/microbiolres14030063","url":null,"abstract":"Acute gastroenteritis (AGE) has a major impact on morbidity and mortality worldwide. The viral aetiology of diarrhoeal diseases may remain unknown due to limited diagnostic facilities. Non-polio enteroviruses (NPEVs) are the third most frequent pathogen detected in stool specimens from AGE cases, yet their potential role in AGE is uncertain. In Nepal, limited data are available on NPEVs, due to both the lack of an adequate surveillance program and the availability of tests. The global polio eradication initiative effort of the WHO has eradicated the incidence of poliomyelitis and acute flaccid paralysis (AFP) from many parts of the world, including Nepal. However, cases of AFP associated with NPEVs have been reported in different countries, including the neighbouring India. This study aims to investigate the diarrhoeal stool samples from a birth cohort until the age of 36 months for NPEVs and the genotype diversity of NPEV in community children with diarrhoea. A total of 280 longitudinal diarrhoeal stool samples that were negative for other enteric pathogens were tested using RT-PCRs. NPEVs was detected in 97 stool specimens (34.6%) and were significantly more frequent in infants up to one year of age. This study identified 17 various NPEV types, with the dominating species being Enterovirus B (EV-B). Ten different types of echoviruses were recorded in this study, with the two rare NPEVs B74 and A120. Based on prevalence, seasonality, and diversity, further studies are warranted to investigate the role of enterovirus in diarrhoeal disease.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"4 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89886143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol Efficiency of Rhizospheric Bacillus against the Plant Pathogen Fusarium oxysporum: A Promising Approach for Sustainable Agriculture 根际芽孢杆菌对植物尖孢镰刀菌的生物防治效果:可持续农业的一条有前途的途径

IF 1.5

Microbiology Research Pub Date : 2023-07-13 DOI: 10.3390/microbiolres14030062

Sarah Boulahouat, H. Cherif-Silini, A. Silini, A. C. Bouket, L. Luptakova, Faizah N. Alenezi, L. Belbahri

{"title":"Biocontrol Efficiency of Rhizospheric Bacillus against the Plant Pathogen Fusarium oxysporum: A Promising Approach for Sustainable Agriculture","authors":"Sarah Boulahouat, H. Cherif-Silini, A. Silini, A. C. Bouket, L. Luptakova, Faizah N. Alenezi, L. Belbahri","doi":"10.3390/microbiolres14030062","DOIUrl":"https://doi.org/10.3390/microbiolres14030062","url":null,"abstract":"Among plant disease management strategies, biological control is a sustainable alternative to the use of chemicals for the control of vascular wilt caused by Fusarium oxysporum. Fusarium wilt is the most devastating disease affecting a wide variety of plants. Bacillus species are the most widely used biological control candidates for the control of these fungal diseases. This review describes the pathogenicity of F. oxysporum, its virulence mechanisms, and host plant–pathogen interactions. The control means deployed by Bacillus species inhibit or kill these phytopathogens. Bacillus spp. produce a wide range of secondary metabolites, including volatile and non-volatile organic compounds. Biocontrol potential is achieved through direct antimicrobial activity, the induction of the host plant’s immune response (Induced Systemic Resistance), and competition for nutrients and space. In addition, parameters governing the selection of effective biocontrol agents and their survival in plant microbial communities are discussed. The influence of the microbiota on the establishment and development of biocontrol agents can assess the potential of these treatments and facilitate the development of effective biopesticides during their field application.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"19 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72534822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evidence of Brucellosis in Hospitalized Patients of Selected Districts of Punjab, Pakistan 巴基斯坦旁遮普选定地区住院患者中布鲁氏菌病的证据

IF 1.5

Microbiology Research Pub Date : 2023-07-10 DOI: 10.3390/microbiolres14030061

Amna Azam, Shahid Saleem Pall, I. Khan, Waqas Ahmad, T. Jamil, Aziz Ur Rehman, M. Imran, W. Shehzad, F. Melzer, M. F. Qamar, H. El-Adawy

{"title":"Evidence of Brucellosis in Hospitalized Patients of Selected Districts of Punjab, Pakistan","authors":"Amna Azam, Shahid Saleem Pall, I. Khan, Waqas Ahmad, T. Jamil, Aziz Ur Rehman, M. Imran, W. Shehzad, F. Melzer, M. F. Qamar, H. El-Adawy","doi":"10.3390/microbiolres14030061","DOIUrl":"https://doi.org/10.3390/microbiolres14030061","url":null,"abstract":"Brucellosis is a bacterial zoonosis worldwide and a potential health risk in Pakistan due to socio-economic reasons and lack of awareness, veterinary health and modern dairy-processing facilities. The aim of this study was to determine the burden of brucellosis in hospitalized patients of selected districts of Punjab, Pakistan. To this end, a total of 280 serum samples were collected from suspected patients in districts Lahore, Okara and Khushab between 2016 and 2018. Anti-Brucella antibodies were detected in 89 (31.8%) sera by Rose Bengal plate test (RBPT), and Brucella abortus DNA was amplified by polymerase chain reaction (PCR) in 42 (15%) samples. In univariable analysis, the seroprevalence varied significantly (p < 0.05) between districts with Lahore indicating 41/101 (40.6%) Odds ratio (OR) = 53.98 (7.22–403.67; 95% CI), Okara 47/99 (47.5%) OR = 71.4 (9.55–533.65; 95% CI) and Khushab 1/80 (1.3%) as reference. Similarly, exposure to aborted materials and a history of blood transfusion did associate significantly (p < 0.05), posing OR = 2.91 (1.58–5.36; 95% CI) and OR = 4.48 (2.56–7.84; 95% CI) risk. However, consumption of raw milk and history of blood transfusion did associate significantly (p < 0.05) in multivariable analysis indicating OR = 1.91 (1.09–3.34; 95% CI) and OR = 4.92 (2.76–8.75; 95% CI), respectively. The association of history of blood transfusion as a brucellosis risk factor underscores the need to create brucellosis awareness programs both at public and institutional levels in the medical and veterinary fields, as well as facilities and capacity building to delve deeper into the epidemiology of brucellosis in the country. Particular attention should be paid to chronically infected patients. Therefore, laboratory modernization, standardization and validation of the diagnostic tests, and staff trainings are required. Finally, pasteurization of the milk is recommended before consumption.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"168 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87945903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Fructooligosaccharides (FOS) on the Immune Response of the Shrimp Penaeus vannamei and on the Reduction in Vibrio spp. and Pseudomonas spp. in Cultures of Post-Larvae 低聚果糖(FOS)对凡纳滨对虾(Penaeus vannamei)免疫反应及培养后弧菌和假单胞菌减少的影响

IF 1.5

Microbiology Research Pub Date : 2023-07-08 DOI: 10.3390/microbiolres14030060

Yulaine Corrales Barrios, A. Roncarati, L. D. Martín Ríos, Maikelis Rodríguez González, Marbelys González Salotén, Yeidel López Zaldívar, A. Arenal

{"title":"Effects of Fructooligosaccharides (FOS) on the Immune Response of the Shrimp Penaeus vannamei and on the Reduction in Vibrio spp. and Pseudomonas spp. in Cultures of Post-Larvae","authors":"Yulaine Corrales Barrios, A. Roncarati, L. D. Martín Ríos, Maikelis Rodríguez González, Marbelys González Salotén, Yeidel López Zaldívar, A. Arenal","doi":"10.3390/microbiolres14030060","DOIUrl":"https://doi.org/10.3390/microbiolres14030060","url":null,"abstract":"Penaeus spp. are the most cultivated type of shrimp because they have rapid growth and good adaptation to farming conditions. Due to diseases that result in high mortality and a decreased product quality, the cultivation of these shrimp globally, and in Cuba in particular, comes with the risk of significant financial losses. This study examined the effect of fructooligosaccharide (FOS, 1-kestose) on the growth and immune response of shrimp, as well as the multitude of Vibrio spp. and Pseudomonas spp. in Penaeus vannamei post-larvae under culture conditions. Fructooligosaccharide was applied in a completely randomized manner at a concentration of 0.4%, in both experimental groups with seventeen tanks each. In the results of this investigation, animals of greater weight (control 6.8 ± 0.2 mg; FOS 9.5 ± 0.3 mg; p < 0.001), length (control 1.1 ± 0.1 mm; FOS 1.3 ± 0.1 mm; p < 0.001), and survival (control 61.7% (95% CI of median 54.2–70.0); FOS 76.6% (95% CI of median 72.1–84.2); p < 0.001) were obtained when administered FOS relative to the control. An increase in the activity of proteases (p < 0.001), enzymes of the innate immune system such as phenoloxidase (p < 0.001), and lysozymes (p < 0.001) was observed, as well as an increase in the number of lectins (p < 0.001). Changes in the microbiota could be observed, with a reduction in Vibrio spp. and Pseudomonas spp. (control 2.4 × 103 ± 0.5 × 103; FOS 1.1 × 102 ± 0.3 × 102; p < 0.001). FOS improves the quality of the post-larvae of P. vannamei as reflected in the length, weight, and survival of the animals. Moreover, FOS stimulates the P. vannamei immune system through the enzymatic activities of phenoloxidase, lysozyme, and a number of lectins. The reduction in the population load of Vibrio spp. and Pseudomonas spp. might be a consequence of the improvement in the quality and immune system of P. vannamei.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"37 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75856902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3L, Three-Lactobacilli on Recovering of Microbiome and Immune-Damage by Cyclophosphamide Chemotherapy—A Pilot Experiment in Rats— 3 -乳酸菌对环磷酰胺化疗大鼠微生物组和免疫损伤的恢复作用

IF 1.5

Microbiology Research Pub Date : 2023-07-06 DOI: 10.3390/microbiolres14030059

Shousong Yue, Zhenzhong Zhang, F. Bian, Yan Zhang, Gao Chen, Youfeng Zhu, Jun Li, J. Picimbon

引用次数: 0