Effects of Fructooligosaccharides (FOS) on the Immune Response of the Shrimp Penaeus vannamei and on the Reduction in Vibrio spp. and Pseudomonas spp. in Cultures of Post-Larvae

IF 2.1 Q3 MICROBIOLOGY

Microbiology Research Pub Date : 2023-07-08 DOI:10.3390/microbiolres14030060

Yulaine Corrales Barrios, A. Roncarati, L. D. Martín Ríos, Maikelis Rodríguez González, Marbelys González Salotén, Yeidel López Zaldívar, A. Arenal

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引用次数: 0

Abstract

Penaeus spp. are the most cultivated type of shrimp because they have rapid growth and good adaptation to farming conditions. Due to diseases that result in high mortality and a decreased product quality, the cultivation of these shrimp globally, and in Cuba in particular, comes with the risk of significant financial losses. This study examined the effect of fructooligosaccharide (FOS, 1-kestose) on the growth and immune response of shrimp, as well as the multitude of Vibrio spp. and Pseudomonas spp. in Penaeus vannamei post-larvae under culture conditions. Fructooligosaccharide was applied in a completely randomized manner at a concentration of 0.4%, in both experimental groups with seventeen tanks each. In the results of this investigation, animals of greater weight (control 6.8 ± 0.2 mg; FOS 9.5 ± 0.3 mg; p < 0.001), length (control 1.1 ± 0.1 mm; FOS 1.3 ± 0.1 mm; p < 0.001), and survival (control 61.7% (95% CI of median 54.2–70.0); FOS 76.6% (95% CI of median 72.1–84.2); p < 0.001) were obtained when administered FOS relative to the control. An increase in the activity of proteases (p < 0.001), enzymes of the innate immune system such as phenoloxidase (p < 0.001), and lysozymes (p < 0.001) was observed, as well as an increase in the number of lectins (p < 0.001). Changes in the microbiota could be observed, with a reduction in Vibrio spp. and Pseudomonas spp. (control 2.4 × 103 ± 0.5 × 103; FOS 1.1 × 102 ± 0.3 × 102; p < 0.001). FOS improves the quality of the post-larvae of P. vannamei as reflected in the length, weight, and survival of the animals. Moreover, FOS stimulates the P. vannamei immune system through the enzymatic activities of phenoloxidase, lysozyme, and a number of lectins. The reduction in the population load of Vibrio spp. and Pseudomonas spp. might be a consequence of the improvement in the quality and immune system of P. vannamei.

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低聚果糖(FOS)对凡纳滨对虾(Penaeus vannamei)免疫反应及培养后弧菌和假单胞菌减少的影响

对虾是养殖最多的虾类，因为它们生长迅速，对养殖条件适应良好。由于疾病导致高死亡率和产品质量下降，这些虾在全球，特别是在古巴的养殖面临重大经济损失的风险。本研究考察了低聚果糖(FOS, 1-酮糖)在培养条件下对凡纳滨对虾(Penaeus vannamei)幼虫生长和免疫反应的影响，以及对弧菌和假单胞菌数量的影响。低聚果糖以完全随机方式施用，浓度为0.4%，两组各17罐。在本研究结果中，体重较大的动物(对照组6.8±0.2 mg;FOS 9.5±0.3 mg;P < 0.001)，长度(对照1.1±0.1 mm;FOS 1.3±0.1 mm;p < 0.001)，生存率(对照组61.7% (95% CI为中位数54.2-70.0);FOS 76.6% (95% CI中位数72.1 ~ 84.2);p < 0.001)。观察到蛋白酶(p < 0.001)、先天免疫系统酶如酚氧化酶(p < 0.001)和溶菌酶(p < 0.001)活性的增加，以及凝集素数量的增加(p < 0.001)。观察到菌群的变化，弧菌和假单胞菌减少(对照组2.4 × 103±0.5 × 103;Fos 1.1 × 102±0.3 × 102;P < 0.001)。果寡糖提高了凡纳滨拟虫幼虫后期的质量，体现在动物的长度、重量和存活率上。此外，FOS通过酚氧化酶、溶菌酶和一些凝集素的酶活性刺激凡纳梅的免疫系统。弧菌和假单胞菌种群负荷的减少可能是由于南美假单胞菌质量和免疫系统的改善。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.