Microbiology Research最新文献_第8页

Trichoderma Species Problematic to the Commercial Production of Pleurotus in Italy: Characterization, Identification, and Methods of Control 意大利平菇商业化生产中存在问题的木霉种类:特征、鉴定和控制方法

Microbiology Research Pub Date : 2023-09-12 DOI: 10.3390/microbiolres14030088

Nadia Lombardi, Angela Pironti, Gelsomina Manganiello, Roberta Marra, Francesco Vinale, Stefania Vitale, Matteo Lorito, Sheridan Lois Woo

{"title":"Trichoderma Species Problematic to the Commercial Production of Pleurotus in Italy: Characterization, Identification, and Methods of Control","authors":"Nadia Lombardi, Angela Pironti, Gelsomina Manganiello, Roberta Marra, Francesco Vinale, Stefania Vitale, Matteo Lorito, Sheridan Lois Woo","doi":"10.3390/microbiolres14030088","DOIUrl":"https://doi.org/10.3390/microbiolres14030088","url":null,"abstract":"Nine isolates of Trichoderma were obtained from the diverse phases of compost preparation and the production of different commercial varieties of oyster mushrooms Pleurotus spp. with the apparent presence of green mould disease. The isolates were morphologically and genetically characterized. Molecular fingerprinting indicated that the isolates belonged to the species T. pleuroticola and T. harzianum. In order to identify control measures, changes in temperature, pH, and application of fungicides were tested on the present isolates, using known Trichoderma biocontrol strains as a reference. Fungicide effects on the growth of Pleurotus isolates were also assessed. The optimal growth temperatures were 25 °C for Trichoderma isolates and 28 °C for Pleurotus isolates, and Trichoderma always grew faster than Pleurotus. In particular, a reduction of about 30% was recorded for some of the Trichoderma isolates when comparing the colony growth at 25 and 28 °C. Trichoderma isolates developed well within a wide pH range, with the best growth occurring between pH 5 and 7, whereas Pleurotus preferred more alkaline conditions (pH 8 to 9). Prochloraz and metrafenone were found to inhibit Trichoderma growth with different dose responses that did not affect the growth of Pleurotus spp. In particular, metrafenone was the most effective active ingredient that inhibited the majority of the Trichoderma isolates (1–25% growth reduction).","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135885467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Potency and Harnessing the Antibacterial Activities of Plant Oils against Foodborne Pathogens 揭示植物油对食源性病原菌的抑菌活性并加以利用

Microbiology Research Pub Date : 2023-09-09 DOI: 10.3390/microbiolres14030087

Vijaya Samoondeeswari Selvarajan, Ramganesh Selvarajan, Jeevan Pandiyan, Akebe Luther King Abia

{"title":"Unveiling the Potency and Harnessing the Antibacterial Activities of Plant Oils against Foodborne Pathogens","authors":"Vijaya Samoondeeswari Selvarajan, Ramganesh Selvarajan, Jeevan Pandiyan, Akebe Luther King Abia","doi":"10.3390/microbiolres14030087","DOIUrl":"https://doi.org/10.3390/microbiolres14030087","url":null,"abstract":"The rising concerns regarding antibiotic resistance and the harmful effects of synthetic preservatives have led to an increasing interest in exploring natural alternatives. Plant oils have been traditionally used for their antimicrobial properties, but systematic investigations into their efficacy against foodborne pathogens are necessary for potential applications in food preservation. This study aimed to evaluate the antibacterial potential of various plant oils (neem, coconut, castor, and olive oil) against common foodborne pathogens and analyze their chemical composition using gas chromatography–mass spectrometry (GC-MS). The oils were tested against foodborne pathogens using the disk diffusion method. Minimum inhibitory concentrations (MICs) were determined to assess the potency of the oils. GC-MS was employed to identify the compounds present in each oil. Neem oil exhibited significant antibacterial activity against all tested pathogens, with pronounced effects against Staphylococcus aureus and Bacillus cereus. Coconut oil showed notable activity against Listeria monocytogenes. Castor oil displayed moderate activity, while olive oil exhibited minimal antibacterial effects. The GC-MS analysis revealed a diverse array of compounds in neem oil, which is likely to contribute to its potent antibacterial properties. Neem and coconut oils, owing to their rich bioactive components, emerged as promising candidates for the development of natural antimicrobial agents. These brief findings support the potential application of plant oils in food preservation and emphasize the need for further research into understanding the underlying mechanisms and optimizing their use.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"168 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136192012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Molecular Epidemiology of Clostridioides difficile Infection in Central India: A Prospective Observational Cohort Study 印度中部艰难梭菌感染的分子流行病学:一项前瞻性观察队列研究

Microbiology Research Pub Date : 2023-09-08 DOI: 10.3390/microbiolres14030086

Rima Biswas, Nick Pinkham, Seth T. Walk, Qian Wang, Shrikant Ambalkar, Ashish R. Satav, Mark H. Wilcox, Rahul Reghunath, Kiran Chawla, Padmaja A. Shenoy, Amit R. Nayak, Aliabbas A. Husain, Dhananjay V. Raje, Rajpal Singh Kashyap, Tanya M. Monaghan

{"title":"The Molecular Epidemiology of Clostridioides difficile Infection in Central India: A Prospective Observational Cohort Study","authors":"Rima Biswas, Nick Pinkham, Seth T. Walk, Qian Wang, Shrikant Ambalkar, Ashish R. Satav, Mark H. Wilcox, Rahul Reghunath, Kiran Chawla, Padmaja A. Shenoy, Amit R. Nayak, Aliabbas A. Husain, Dhananjay V. Raje, Rajpal Singh Kashyap, Tanya M. Monaghan","doi":"10.3390/microbiolres14030086","DOIUrl":"https://doi.org/10.3390/microbiolres14030086","url":null,"abstract":"This prospective observational cohort study aimed to establish and compare baseline rates of Clostridioides difficile infection (CDI) in community and hospitalized patients in Nagpur and rural Melghat Maharashtra, including adults aged ≥18 years with a diagnosis of diarrhoea as defined as 3 or more loose stools in a 24 h period. All diarrhoeal samples were tested for CDI using the C. diff Quik Chek Complete enzyme immunoassay. C. difficile-positive stool samples were characterised by toxigenic culture, antimicrobial susceptibility testing and PCR ribotyping. C. difficile testing was performed on 1683 patients with acute diarrhoea. A total of 54 patients (3.21%; 95% CI: 2.42–4.17) tested positive for both the GDH antigen and free toxin. The risk factors for CDI included the presence of co-morbidities, antibiotic usage, and immunosuppression. The detected PCR ribotypes included 053-16, 017, 313, 001, 107, and 216. Our findings show that toxigenic C. difficile is an important but neglected aetiologic agent of infective diarrhoea in Central India. These results underscore the need to enhance the awareness and testing of patients with diarrhoea in India regarding the presence of toxigenic C. difficile, particularly in high-risk individuals with multiple co-morbidities, immunosuppression, and recent or ongoing antibiotic exposure or hospitalization.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136362378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Compounds from Balanites aegyptiaca against Squalene Epoxidase of Micropsorum gypseum—In Vitro and In Silico Studies 埃及巴兰类化合物对石膏微孢菌角鲨烯环氧化酶的体外和硅质研究

IF 1.5

Microbiology Research Pub Date : 2023-09-04 DOI: 10.3390/microbiolres14030085

Mohamed Hussain Syed Abuthakir, V. Hemamalini, Reham M. Alahmadi, Anis Ahamed, A. A. Hatamleh, Razack Abdullah, Jeyam Muthusamy

{"title":"Evaluation of Compounds from Balanites aegyptiaca against Squalene Epoxidase of Micropsorum gypseum—In Vitro and In Silico Studies","authors":"Mohamed Hussain Syed Abuthakir, V. Hemamalini, Reham M. Alahmadi, Anis Ahamed, A. A. Hatamleh, Razack Abdullah, Jeyam Muthusamy","doi":"10.3390/microbiolres14030085","DOIUrl":"https://doi.org/10.3390/microbiolres14030085","url":null,"abstract":"Microsporum gypseum is a dermatophyte with a geophilic nature that is found all over the globe. It mainly causes tinea in the scalp, arms, and legs in humans. Squalene epoxidase (SE) is a crucial enzyme in M. gypseum for the biosynthesis of ergosterol. The medicinal plant Balanites aegyptiaca is an abundant supply of secondary constituents with great therapeutic values. In this research, the fruit epicarp portion was used to inhibit M. gypseum using experimental and computational techniques. The anti-dermatophytic activity of epicarp extracts on M. gypseum was evaluated using the poison plate method at five different concentrations. At 3 mg/mL, the M. gypseum was completely controlled by the fractioned chloroform extract of epicarp. The compounds from previous research were utilized for docking studies (Abuthakir et al., 2022). The ideal compounds and the drug terbinafine were then docked using Schrödinger’s Glide module. It demonstrates that (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside outperforms other substances and the drug terbinafine in docking analysis. Desmond, Schrödinger Molecular Dynamics simulations were also performed for (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside-squalene epoxidase complexes. The complex appears to be more stable, according to the MD simulation research. This study indicates that (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside could be used as a potential inhibitor of M. gypseum growth, and it could be studied further.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"55 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86766247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular Mycorrhizal Fungus Funneliformis mosseae Improves Soybean Growth Even in Soils with Good Nutrition 丛枝菌根真菌即使在营养良好的土壤中也能促进大豆的生长

IF 1.5

Microbiology Research Pub Date : 2023-08-31 DOI: 10.3390/microbiolres14030084

V. Řezáčová, Ema Némethová, I. Stehlíková, A. Czakó, M. Gryndler

{"title":"Arbuscular Mycorrhizal Fungus Funneliformis mosseae Improves Soybean Growth Even in Soils with Good Nutrition","authors":"V. Řezáčová, Ema Némethová, I. Stehlíková, A. Czakó, M. Gryndler","doi":"10.3390/microbiolres14030084","DOIUrl":"https://doi.org/10.3390/microbiolres14030084","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) improve plant growth and may be useful in maintaining and even restoring soil. However, data on the latter function are sparse and only indirect, which is especially true for conventional management conditions with adequate nutrient availability. Our study focused on utilizing the prevalent AMF species, Funneliformis mosseae, to enhance Glycine max production, while also exploring its partly explored impact on soil aggregation. Working in greenhouse conditions, we examined whether, in a nutrient-sufficient environment, AMF would improve crop biomass accumulation and nutrition, as well as the stability of soil aggregates (SAS). We also looked for a synergistic effect of dual inoculation using AMF and symbiotic rhizobium. Plants were or were not inoculated with AMF or Bradyrhizobium japonicum in a two-factorial design. AMF inoculation increased soybean biomass, but AMF inoculation had no impact on P and N input to the shoots. Mycorrhiza did not affect either glomalin abundance or SAS. All the impacts were, however, independent from rhizobial inoculation, which was ineffective in this nutrient-available environment. Our assay suggests that arbuscular mycorrhiza may have a positive effect on soybean growth even under conventional management with adequate nutrition. The positive effects of AMF on soybean growth, together with the fact that AMF generally do not thrive in good nutrient availability, should be taken into account when planning mineral fertilization levels.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"5 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78943224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Assessment of MCR-1 Gene among Pandrug-Resistant Acinetobacter baumannii 泛耐药鲍曼不动杆菌MCR-1基因的分子鉴定

IF 1.5

Microbiology Research Pub Date : 2023-08-30 DOI: 10.3390/microbiolres14030083

Shaimaa S. Sobieh, S. A. Mohamed, Manal A. El-Sayed, S. Abdallah

{"title":"Molecular Assessment of MCR-1 Gene among Pandrug-Resistant Acinetobacter baumannii","authors":"Shaimaa S. Sobieh, S. A. Mohamed, Manal A. El-Sayed, S. Abdallah","doi":"10.3390/microbiolres14030083","DOIUrl":"https://doi.org/10.3390/microbiolres14030083","url":null,"abstract":"Background Antimicrobial resistance has become one of the most widespread threats to humans. Acinetobacter baumannii is one of the pathogens responsible for healthcare-associated infections (nosocomial). Colistin is considered the last resort antibiotic against infections with pandrug-resistant (PDR) pathogens. Results: Eleven isolates were detected phenotypically as PDR A. baumannii and were confirmed molecularly using 16S rDNA. The MCR-1 gene was not detected within the chromosomal DNA of the selected isolates. Plasmid bearing the MCR-1 gene was identified in 10 selected isolates of A. baumannii that had not been previously observed to carry the MCR-1 gene. Moreover, the use of colistin in combination with anionic antibiotics or natural compound pterostilbene poses a viable therapeutic alternative for PDR and revives colistin’s bactericidal effects on MCR-1-positive A. baumannii. Finally, the transmission electron microscopy studies proved the synergistic effect of these combinations and revealed the disruption of resistant A. baumannii’s outer membrane and alteration of the permeability properties that allowed overcoming the resistance of the isolates to colistin. Conclusions: Antimicrobial resistance of A. baumannii is related to the presence of the transferable plasmid-bearing MCR-1 gene. This study proved the ability of the combinations of colistin with anionic antibiotics and/or natural compound pterostilbene to restore the bactericidal effect of colistin. Overall, these combinations could be novel promising clinical alternatives against the increasing threat of the widespread multidrug-resistant A. baumannii.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83825520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Paclobutrazol and Rhizobacterium Variovorax sp. YNA59 Effects on Mitigating Drought Stress in Chinese Cabbage (Brassica rapa ssp. Pekinensis) 多效唑与变ovorax根杆菌YNA59对大白菜抗旱效果的比较评价。学报)

IF 1.5

Microbiology Research Pub Date : 2023-08-24 DOI: 10.3390/microbiolres14030081

Sang-mo Kang, A. Adhikari, Yu-na Kim, In-Jung Lee

{"title":"Comparative Evaluation of Paclobutrazol and Rhizobacterium Variovorax sp. YNA59 Effects on Mitigating Drought Stress in Chinese Cabbage (Brassica rapa ssp. Pekinensis)","authors":"Sang-mo Kang, A. Adhikari, Yu-na Kim, In-Jung Lee","doi":"10.3390/microbiolres14030081","DOIUrl":"https://doi.org/10.3390/microbiolres14030081","url":null,"abstract":"Drought has severely impacted worldwide agricultural land, resulting in substantial yield loss and abiotic pressures in crops such as Chinese cabbage. Paclobutrazol (PBZ) and plant growth-stimulating bacteria have been extensively recommended to reduce a variety of stresses in crops. Considering these facts, we used PBZ and rhizobacterium Variovorax sp. YNA59, a microbe with potential plant growth-stimulating qualities, in Chinese cabbage under drought stress and non-stressed conditions, and we observed their impacts on morphological and physiological attributes. Our findings revealed a considerable improvement in total plant biomass growth after treatment with YNA59 (28%) and PBZ (8%) under drought conditions. In contrast, negative results of PBZ were observed under normal conditions, suppressing plant growth and reducing plant biomass. The increase in physiological parameters, such as chlorophyll content, relative water content, and photochemical efficiencies of PS II, were observed in YNA59-treated plants, followed by PBZ, especially under drought. Furthermore, drought stress significantly increased the endogenous phytohormone abscisic acid level, whereas PBZ and YNA59 inoculations significantly decreased it. Antioxidant analysis revealed that PBZ and YNA increased glutathione and catalase and decreased polyphenol oxidase and peroxidase levels. These findings suggested that rhizobacterium YNA59 could be more significant for conferring water stress in the Chinese cabbage plant than PBZ.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"3 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81793473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacilli Profile in Faecal Samples of Tunisian Children Diagnosed with Autism Spectrum Disorder 诊断为自闭症谱系障碍的突尼斯儿童粪便样本中的乳酸杆菌谱

IF 1.5

Microbiology Research Pub Date : 2023-08-24 DOI: 10.3390/microbiolres14030082

Mariem Chamtouri, A. Merghni, N. Gaddour, M. Mastouri, S. Arboleya, C. G. de los Reyes-Gavilán

{"title":"Lactobacilli Profile in Faecal Samples of Tunisian Children Diagnosed with Autism Spectrum Disorder","authors":"Mariem Chamtouri, A. Merghni, N. Gaddour, M. Mastouri, S. Arboleya, C. G. de los Reyes-Gavilán","doi":"10.3390/microbiolres14030082","DOIUrl":"https://doi.org/10.3390/microbiolres14030082","url":null,"abstract":"Alterations in faecal lactobacilli in autistic children have been reported, but little is known related to age and disorder severity. We used a culture-based method and partial 16S rRNA gene sequencing to isolate and identify lactobacilli strains from faeces of Tunisian autistic children (ASD group) and compared them with strains isolated from siblings (SIB) and children from the general population (GP). The ASD group displayed an increased number of different species compared to SIB and GP. Differences in species abundance with age accounted for a significant decrease in the abundance of Lactiplantibacillus plantarum/Lactiplantibacillus pentosus isolates in the GP at the age of 8–10 years compared to the age of 4–7 years, and to a significantly lower abundance of Lacticaseibacillus rhamnosus in the ASD group with respect to SIB and the GP at the age of 8–10 years. Simpson’s and Shannon–Wiener indices showed a more pronounced species diversity increase with age in the GP group compared to the ASD and SIB groups. Minor differences were found in lactobacilli prevalence and in species diversity between children with severe and mild-to-moderate ASD. Overall, we found substantial differences in the profile of faecal lactobacilli species in the ASD and GP groups at the age of 8–10 years.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"94 1 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83503320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Production of Glutathione by Recombinant Escherichia coli K-12 重组大肠杆菌K-12细胞外生产谷胱甘肽的研究

IF 1.5

Microbiology Research Pub Date : 2023-08-23 DOI: 10.3390/microbiolres14030080

Hideyuki Suzuki, Kazuki Nishida, Tatsuya Nakamura

{"title":"Extracellular Production of Glutathione by Recombinant Escherichia coli K-12","authors":"Hideyuki Suzuki, Kazuki Nishida, Tatsuya Nakamura","doi":"10.3390/microbiolres14030080","DOIUrl":"https://doi.org/10.3390/microbiolres14030080","url":null,"abstract":"The goal of this study was to produce a sufficient amount of glutathione in the fermentation medium without the addition of cysteine. This would simplify and reduce the cost of its purification. In addition to reducing the cost of cysteine, it also avoids the inhibition of bacterial growth by cysteine. The gshA, gshB, and cysE genes of Escherichia coli were cloned under the control of the strong T5 promoter of the pQE-80L plasmid and introduced into an E. coli strain knocked out for the genes encoding γ-glutamyltranspeptidase and the GsiABCD glutathione transporter, which are responsible for the recycling of excreted glutathione. The overexpression of the gshA and gshB genes, genes for γ-glutamylcysteine synthetase and glutathione synthetase, and the cysEV95R D96P gene, a gene for serine acetyltransferase with the V95R D96P mutation that makes it insensitive to cysteine, were effective on glutathione production. Na2S2O3 was a good sulfur source for glutathione production, while the addition of Na2SO4 did not affect the glutathione production. With the addition of 50 mM glutamic acid and 75 mM glycine, but without the addition of cysteine, to the simplified SM1 medium, 4.6 mM and 0.56 mM of the reduced and oxidized glutathione, respectively, were accumulated in the extracellular space after 36 h of batch culture. This can eliminate the need to extract glutathione from the bacterial cells for purification.","PeriodicalId":43788,"journal":{"name":"Microbiology Research","volume":"18 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88596655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Recombinant Escherichia coli with a High L-Phenylalanine Production Yield from Glucose 葡萄糖高产l -苯丙氨酸重组大肠杆菌的构建

IF 1.5

Microbiology Research Pub Date : 2023-08-21 DOI: 10.3390/microbiolres14030079

Pengfei Gu, Shuo Zhao, Chengwei Li, Shuixing Jiang, Hao Zhou, Qiang Li

引用次数: 0