The Molecular Epidemiology of Enterovirus in a Birth Cohort in Nepal

IF 2.1 Q3 MICROBIOLOGY

Microbiology Research Pub Date : 2023-07-20 DOI:10.3390/microbiolres14030063

S. Shrestha, J. Shrestha, T. Strand, S. Numanovic, A. Andreassen, J. Dembinski, R. Vikse, S. Dudman

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Abstract

Acute gastroenteritis (AGE) has a major impact on morbidity and mortality worldwide. The viral aetiology of diarrhoeal diseases may remain unknown due to limited diagnostic facilities. Non-polio enteroviruses (NPEVs) are the third most frequent pathogen detected in stool specimens from AGE cases, yet their potential role in AGE is uncertain. In Nepal, limited data are available on NPEVs, due to both the lack of an adequate surveillance program and the availability of tests. The global polio eradication initiative effort of the WHO has eradicated the incidence of poliomyelitis and acute flaccid paralysis (AFP) from many parts of the world, including Nepal. However, cases of AFP associated with NPEVs have been reported in different countries, including the neighbouring India. This study aims to investigate the diarrhoeal stool samples from a birth cohort until the age of 36 months for NPEVs and the genotype diversity of NPEV in community children with diarrhoea. A total of 280 longitudinal diarrhoeal stool samples that were negative for other enteric pathogens were tested using RT-PCRs. NPEVs was detected in 97 stool specimens (34.6%) and were significantly more frequent in infants up to one year of age. This study identified 17 various NPEV types, with the dominating species being Enterovirus B (EV-B). Ten different types of echoviruses were recorded in this study, with the two rare NPEVs B74 and A120. Based on prevalence, seasonality, and diversity, further studies are warranted to investigate the role of enterovirus in diarrhoeal disease.

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尼泊尔出生队列肠病毒的分子流行病学研究

急性胃肠炎(AGE)在世界范围内对发病率和死亡率有重大影响。由于诊断设施有限，腹泻病的病毒病因可能仍然未知。非脊髓灰质炎肠道病毒(npev)是AGE病例粪便标本中检测到的第三大常见病原体，但其在AGE中的潜在作用尚不确定。在尼泊尔，由于缺乏适当的监测规划和可获得的检测，关于非机动车的数据有限。世界卫生组织的全球根除脊髓灰质炎行动努力已经在世界许多地方，包括尼泊尔，消除了脊髓灰质炎和急性弛缓性麻痹的发病率。然而，AFP与npev相关的病例已在不同国家报告，包括邻国印度。本研究旨在调查来自出生队列的腹泻粪便样本，直到36个月的NPEV和社区腹泻儿童NPEV的基因型多样性。使用rt - pcr检测了280份其他肠道病原体呈阴性的纵向腹泻粪便样本。在97份粪便标本中检测到npev(34.6%)，并且在一岁以下的婴儿中更为常见。本研究确定了17种不同的NPEV类型，主要种是肠病毒B (EV-B)。本研究记录了10种不同类型的埃可病毒，其中包括两种罕见的npev B74和A120。基于患病率、季节性和多样性，有必要进一步研究肠道病毒在腹泻病中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.