Journal of medical and bioengineering最新文献

{"title":"Development of Hairy Root Culture of Andrographis Paniculata for in Vitro Adrographollide Production","authors":"Erly Marwani, D. Pratiwi, Karlina Wardhani, R. Esyanti","doi":"10.12720/jomb.4.6.446-450","DOIUrl":"https://doi.org/10.12720/jomb.4.6.446-450","url":null,"abstract":"Development the hairy root culture of A. paniculata was conducted for growing the hairy roots and production of andrographollide. Different strains of Agrobacterium rhizogenes (R-1000, A4, ATCC 15834), different types of explants (cotyledons, hypocotyls, and leaves) and different infection time of A. rhizogenes (1, 2, 3 days) were tested to induce hairy roots of A. paniculata. The results indicated that the best strain, type of explants and infection time for hairy roots induction were found in strain ATCC 15834, the explants of cotyledon and the 2 days of infection, respectively. The best medium for growing the hairy roots was liquid half strength MS medium with the addition of 5.0 μM IBA. The highest amount of andrographollide was observed in the medium with the addition of 5.0 μM IBA on the week of two, as much as 0.54%. Integration of T-DNA of A. rhizogenes in hairy roots was confirmed by polymerase chain reaction (PCR) analysis with specific primer for rolA and rolC genes of the plasmid. Visualization of the PCR products on agarose gel electrophoresis showed two fragments with lengths of 248 bp and 490 bp which corresponds to rolA and rolC genes from Ri plasmids of ATCC 15834. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"306 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121647906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Actigraphic Detection of REM Sleep Based on Respiratory Rate Estimation","authors":"Kawamoto Ken, H. Kuriyama, Seiki Tajima","doi":"10.12720/JOMB.2.1.20-25","DOIUrl":"https://doi.org/10.12720/JOMB.2.1.20-25","url":null,"abstract":"Abstract— The use of wrist actigraphy in sleep research has for long been limited to the classification of sleep/wake; little progress has been made in the evaluation of the sleep stages. We propose and evaluate two novel algorithms: a method for respiratory rate estimation based on spectral analysis of actigraphic data, and a method for estimating REM sleep based on the detected respiratory rates. Using simultaneous recordings of polysomnography and actigraphy data acquired from 34 subjects, we found that our proposed method successfully estimated respiratory rate with low mean absolute error (0.52 counts/min), and REM sleep with high positive predictive value (64.5%), but low sensitivity (11.0%). While the low sensitivity hinders the immediate clinical use of our algorithms, our findings are important in indicating for the first time that actigraphs have the potential to detect REM sleep.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122565398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prioritizing Drivers of Sustainable Competitive Advantages in Green Supply Chain Management Based on Fuzzy AHP","authors":"N. Somsuk","doi":"10.12720/JOMB.3.4.259-266","DOIUrl":"https://doi.org/10.12720/JOMB.3.4.259-266","url":null,"abstract":"Successful implementation of green supply chain management (GSCM) can create a sustainable competitive advantage. A firm’s specific internal and relational resources can be considered as drivers of sustainable competitive advantages in GSCM. To extend the scope of the limited research on the identification and prioritization of resource-related drivers having a strong influence on a firm’s sustainable performance, this study was to explore the priority of these drivers based on the Triple Bottom Line. Fuzzy analytic hierarchy process (AHP) approach was applied to prioritize these drivers from experts’ point of view — the view from the electronics industry in Thailand. Resource-based and relational views were then used to determine the drivers and develop an AHP model for prioritizing such drivers. The model based on our previous research. Finally, managerial and policy recommendations for more effective strategic management tailored to the context of the electronics industry in Thailand were provided.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123742746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Optimised High-Salt CTAB Protocol for Both DNA and RNA Isolation from Succulent Stems of Hylocereus sp.","authors":"Lilian Wong, S. Silvaraj, L. Phoon","doi":"10.12720/jomb.3.4.236-240","DOIUrl":"https://doi.org/10.12720/jomb.3.4.236-240","url":null,"abstract":"The isolation of high quality DNA and RNA from plant species harboring high levels of polysaccharides and secondary metabolites are typically problematic, especially those in cactus. These compounds often co-precipitate with DNA and RNA thus causes low recovery and quality of the nucleic acids. Six DNA extraction protocols were tested on the sample of Hylocereus spp. of which the results were compared and analyzed. For comparison, three manufacturer’s protocols from different commercial kits and another three conventional DNA extraction protocols were compared. It was found that conventional method generally produces consistent and higher yield. Among the conventional protocols itself, each has their pros and cons. Therefore, a modified protocol which is concise, quick and simple was developed for Hylocereus spp. which is beneficial for further molecular work. This method was proven to be reliable in generating a good quality of DNA from these particular genera. Similarly for RNA extraction, four different extraction protocols were tested on the same sample. The results were analyzed and a modified protocol was developed to obtain a higher quality and yield of RNA for further downstream investigations. The extraction buffer from our modified DNA extraction protocol was tested on the RNA extraction and shows a good yield.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128496999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of Palm Oil Mill Effluent in Microbial Fuel Cell Using Polyacrylonitrile Carbon Felt as Electrode","authors":"M. R. Khan, E. Baranitharan, R. PrasadD.M.","doi":"10.12720/JOMB.2.4.252-256","DOIUrl":"https://doi.org/10.12720/JOMB.2.4.252-256","url":null,"abstract":"Palm oil mill effluent (POME) is an organic waste \u0000material produced at the oil palm mills. It is highly polluting due to its high content of biological and chemical oxygen demand. In the present paper, POME was treated using double chamber microbial fuel cell with simultaneous generation of electricity. Polyacrylonitrile carbon felt (PACF) was used as electrode and anaerobic sludge was used as inoculum throughout the MFC experiments. \u0000Various dilutions of raw POME were used to analyze the \u0000MFC power generation, COD removal efficiency and coulombic efficiency. Among the raw POME and different concentrations of POME used, the PACF with raw POME showed the maximum power density and volumetric power density of about 45mW/m2 \u0000and 304mW/m3 respectively but it showed low coulombic efficiency and low COD removal efficiency of about 0.8% and 45% respectively while PACF with 1:50 dilution showed higher COD removal efficiency and coulombic efficiency of about 70% and 24% but showed low power density and low volumetric power density of about 22mW/m2 and 149mW/m3 \u0000respectively. The results show that MFC possesses great potential for the simultaneous treatment of POME and power generation using PACF as electrode.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126967963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity of the Sea Bird Nest (Eucheuma Cottonii) and Its Radical Scavenging Effect on Human Keratinocytes","authors":"C. Lim, R. Koh, T. Haw, Laura A. Boudville","doi":"10.12720/JOMB.4.6.461-465","DOIUrl":"https://doi.org/10.12720/JOMB.4.6.461-465","url":null,"abstract":"The potential of Eucheuma cottonii (EC) to be a novel source of antioxidants and protection against photoageing is increasingly evident but largely unexplored. This study aimed to evaluate the antioxidant activity and radical scavenging capacity of EC extracts on human keratinocytes. Aqueous and methanol extracts from EC were evaluated in a series of in vitro assays on the HaCaT keratinocyte cell line. Antioxidant activity was determined via the DPPH assay, while MTT was used to evaluate the cytotoxicity of EC extracts up to 72h exposure. Quantitative and qualitative DCFH-DA fluorescence assays assessed intracellular reactive oxygen species (ROS) levels in UVirradiated cells. EC extracts at concentrations from 10 μg/ml were found to possess significant antioxidant activity (p<0.05). Interestingly, the aqueous extract compromised cell viability at high concentrations, while the methanol extract was relatively non-toxic. Intracellular ROS levels significantly decreased with increasing concentration of EC extract treatment (p<0.05). In conclusion, EC extracts demonstrated antioxidant activity and protective effects against UV-induced ROS degeneration in keratinocytes, thus underlining its potential in nutraceutical research to promote skin rejuvenation. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134353658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivity, Qualitative and Quantitative Components of Alstonia Boonei Leaf Extracts on Anopheles Mosquito Larvae in Nigeria","authors":"F. Omoya, Kazeem Oladipupo, A. Abe, O. Udensi","doi":"10.12720/JOMB.1.1.39-41","DOIUrl":"https://doi.org/10.12720/JOMB.1.1.39-41","url":null,"abstract":"Malaria the most common disease in Africa is transmitted by the bites of female Anopheles mosquito. Different control measures have been applied to combat the spread of this infection including biological control measures. Our research focused on the use of water, ethanol and methanol extracts of a local medicinal plant; Alstonia boonei for 24 hours in vitro on different instars of mosquito larvae. The qualitative and quantitative components of these plants were also determined. The highest mortality was recorded in the ethanol treated larvae. Lethal concentration (LC) at LC50 and LC75 varied with the different extracts and instars. Phytochemical screening showed the presence of some chemicals that are insecticidal such as tannins, saponin and alkaloids. The quantitative components of these chemicals were also seen to vary significantly. Conclusively, our study revealed that the use of Alstonia boonei as vector control agents can reduce the spread of malaria infection.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134383557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Image Analysis to Quantify the Temporo-Spacial Expression of Cellular Receptors","authors":"Sarmed Al-Samerria, G. Almahbobi","doi":"10.12720/JOMB.3.3.179-182","DOIUrl":"https://doi.org/10.12720/JOMB.3.3.179-182","url":null,"abstract":"� Abstract≤O varian folliculogenesis is primarily controlled by the action of gonadotropins namely follicle stimulating hormone (FSH) and luteinizing hormone (L H). Several reports indicated that the process of initial recruitment of primordial follicles to the growing follicles is not gonadotropin-dependent but Bone morphogenetic protein (B M P)-dependent. However, this has not been unequivocally confirmed. T he aim of this study was to investigate the temporo-spacial protein expression of the B M P receptors 1B (B M PR1b), FSH R and L H R in several stages of follicle development. W hile the localization of all receptors was found in granulosa cell membrane of the follicles the temporal expression was varied. B M PR1b was expressed in all follicle stages, FSH R was detected in primary follicles onward and L H R was absent in both primordial and primary follicles but appeared in later stages. Q uantitative analysis based on the intensity of fluorescent signals showed that the expression of B M PR1b, FSH R and L H R significantly (p< 0.001 p< 0.0001 p< 0.0001 respectively) increased with follicular development. W e have concluded that the combination of sensitive immunofluorescence labeling and computerized 3D image analysis proves efficient tools for in situ detection and quantification of the expression of small amount of protein in a complex tissue structure.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134212410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic Hydrolysis of Rice Straw: Process Optimization","authors":"L. Ong, C. Chan, A. Chew","doi":"10.12720/JOMB.1.1.14-16","DOIUrl":"https://doi.org/10.12720/JOMB.1.1.14-16","url":null,"abstract":"Rice straw consists of cellulose, hemicellulose and lignin. Cellulose is the most abundant component that present in the rice straw. Through enzymatic hydrolysis of cellulose, fermentable sugars are produced in the present of cellulases. In this study, untreated rice straw that is abundance in Malaysia was used as feedstock for exoglucanase production and enzymatic hydrolysis. Under solid substrate fermentation, exoglucanase was produced by locally isolated Aspergillus niger using untreated rice straw as sole carbon source. Subsequently, the untreated rice straw was hydrolyzed by the extracted exoglucanase, which obtained from solid substrate fermentation (SSF) to optimize the production of fermentable sugar based on three different factors, i.e. initial pH, substrate concentration, and enzyme concentration. An exoglucanase with a maximum activity of 46.45 FPU/g of rice straw was obtained from SSF produced by locally isolated A. niger. The optimum parameters obtained for the hydrolysis were at pH 6.0, 12% (w/v) of substrate concentration and with enzyme activity of 10 U/g of rice straw. The fermentable sugar concentration obtained with these parameters was 3.62 g/L.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"39 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123286677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hospital Satisfaction Survey Report of Taiwan’s Current Implemented Electronic Medical Records (EMRs)","authors":"James Andrew Worley, Chien-Yeh Hsu, Cheng-Mei Chen","doi":"10.12720/JOMB.2.1.75-79","DOIUrl":"https://doi.org/10.12720/JOMB.2.1.75-79","url":null,"abstract":"This article provides empirical data and findings in-regards to Taiwan’s overall hospital EMRs and its EMR systems. In doing so, it aims to document information about the current status of the EMRs systems which have already been implemented and are currently in use in Taiwan. Objectively, this study contains breakthrough information for the international biomedical informatics community because it has been authorized to use the unpublished data and findings from one survey conducted during a major national Taiwanese project conducted by the National Taiwan Department of Health Committee. The title of this project is, “An evaluation on expediting the processing of Taiwan’s heath care plans; which ultimately produced a final report indexed as RDEC-MIS-100-010”. The methodology of this paper and study uses several statistical methods to precisely deduce knowledge about the overall user satisfaction of the hospital participants whom were polled during the above mentioned project.Furthermore, the research and raw data of this paper was then sought to further elaborate on the issue of Taiwan’s EMRs systems by developing this paper. Specifically, this paperhas revealed several insightful findings and statistical results based on analytical scientific methodology performed by using a 21 question hospital satisfaction survey which polled 137 public and private hospitals in Taiwan. Through this collaboration, a total of 782 completed surveys were tallied and provided us an ample amount of data to produce several statistical figures and models to support or coinciding discussion and result portion of this paper. In addition to statistical results about Taiwan’s overall hospital EMRs and EMR systems based on the resulting data, all coming from a 21 question satisfaction survey, this paper also consists of a comparative analysis between both public Taiwanese hospitals vs. private Taiwanese hospitals. Finally, based on the data analysis of this paper, we provide some the possible implications for some of the high and low variance trends observed after processing this information. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"225 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122349222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}