Journal of medical and bioengineering最新文献_第8页

Effects of Different Initial pH of Modified Zarrok Medium on Large-scale Spirulina Maxima Culture 不同初始pH改性Zarrok培养基对螺旋藻大规模培养的影响

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.4.266-269

P. Sornchai, S. Iamtham

{"title":"Effects of Different Initial pH of Modified Zarrok Medium on Large-scale Spirulina Maxima Culture","authors":"P. Sornchai, S. Iamtham","doi":"10.12720/JOMB.2.4.266-269","DOIUrl":"https://doi.org/10.12720/JOMB.2.4.266-269","url":null,"abstract":"The effects of Modified Zarrouk Medium (MZM) with different pH on Spirulina maxima culture were determined using five parameters: the growth rate, lag phase duration, nutrients (protein, carbohydrate and lipid), pigments (chlorophyll-a, betacarotene and phycocyanin), and morphology of the algae. The experiment was divided into 4 treatments. All treatment contained the same MZM; however, different initial pH of 9, 9.5, 10 and 10.5 were applied to the experimental treatment I, II, III and IV, respectively. The parameters of these treatments were compared to those of the positive control (the Zarrouk medium pH 9). The results showed that the algae grown in media treatment I could reduce the duration of lag phase and gave the highest specific growth rate (4.12 0.005 g/l), while the algae grown in media treatment IV provided the lowest specific growth rate (1.09 0.006 g/l). Regarding nutrients, the algae grown in media treatment I provided highest protein content of 71.0±0.06 % and the algae grown in media treatment III provided the highest carbohydrate content of 17.07±0.02 %. It was found that algae grown in media treatment I yielded the best in pigment contents, which were the chlorophyll-a (15.2±0.16 mg/g), betacarotene (3.16±0.32 mg/g) and phycocyanin (46.37±0.20 mg/g). The Morphological studies of algae using microscope SEM and TEM revealed that most trichome of algae grown in media treatment III and group IV were changed from helicoidal to straight.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"87 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125365991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of Partially Purified Peroxidase from Fingerroot (Boesenbergia Rotunda (L.) Mansf.) 手指根(Boesenbergia Rotunda)部分纯化过氧化物酶的研究Mansf)。

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/jomb.4.3.170-177

L. Shank, Pairoje Kijjanapanich, S. Phutrakul, Nattapong Fongbua

{"title":"Characterization of Partially Purified Peroxidase from Fingerroot (Boesenbergia Rotunda (L.) Mansf.)","authors":"L. Shank, Pairoje Kijjanapanich, S. Phutrakul, Nattapong Fongbua","doi":"10.12720/jomb.4.3.170-177","DOIUrl":"https://doi.org/10.12720/jomb.4.3.170-177","url":null,"abstract":"Peroxidase from fingerroot was partially purified and characterized for potential use in analytical applications. The procedure began with crude extract preparation, followed by ammonium sulfate fractionation and concanavalin A-sepharose 4B affinity chromatography. The fraction of protein precipitated at 20-40% saturation of ammonium sulfate possessed the highest specific activity of 7.74 units/ mg. This fraction was subsequently purified further using affinity binding of peroxidase, a glycosylated enzyme, to Concanavalin A-sepharose 4B column. The chromatographic step produced peroxidase with specific activity of 55.33 units/ mg and resulted in 19.34 fold of purification. Invetigation on optimal conditions revealed pH optimum to be at 6 and temperature optimum to be at 40 °C. After 5 hour incubation fingerroot peroxidase retained 60% of activity at pH 6 and 40 °C. Activity of the enzyme rapidly dropped at pH 2, while temperature at 70 °C and above inactivated the enzyme within the first hour. At concentration of 5 mM CaCl2, MgCl2, MnCl2, NaCl and ZnCl2 did not show notable effect on peroxidase activity, whereas CuCl2 and FeCl2 moderately inhibited the activity of peroxidase. AlCl3 and FeCl3 at 5 mM highly inhibited the activity of the enzyme up to 70%. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122863307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Salivary Antioxidative Index in Newborns at Risk of Sepsis as Novel Parameter for Early-Onset Neonatal Sepsis 新生儿脓毒症风险的唾液抗氧化指数作为早发型新生儿脓毒症的新参数

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.1.63-66

A. Yunanto, Rizky Taufan Firdaus, T. Triawanti, E. Suhartono

引用次数: 6

Discrimination of Oral Mucosal Disease Inspired by Diagnostic Process of Specialist 专家诊断过程对口腔黏膜疾病鉴别的启示

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.1.57-61

Yusaku Nishi, K. Horio, Kentaro Saito, M. Habu, K. Tominaga

引用次数: 10

Dual Carbon Fermentation for the Production of Inducible Cellobiohydrolase by Recombinant Aspergillus Niger 重组黑曲霉双碳发酵生产诱导型纤维素生物水解酶的研究

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.2.93-97

N. Othman, J. Jahim, A. Murad, F. Bakar

{"title":"Dual Carbon Fermentation for the Production of Inducible Cellobiohydrolase by Recombinant Aspergillus Niger","authors":"N. Othman, J. Jahim, A. Murad, F. Bakar","doi":"10.12720/JOMB.3.2.93-97","DOIUrl":"https://doi.org/10.12720/JOMB.3.2.93-97","url":null,"abstract":" Abstract—This study investigates the use of two carbon sources in fermentation for the production of cellobiohydrolase (CBH), an adaptive or inducible enzyme. Selection of carbon source was first done using single substrate cultivation between glucose, maltose and lactose using recombinant A. niger PY11. This recombinant microorganism will produce CBH in high quantity, with maltose acting as its main inducer. Glucose and maltose were selected in dual carbon fermentation, as glucose produce highest biomass 0.32g/ml at day 4 th and maltose produce 0.42g/ml biomass at day 9 th . Cultivation of A. niger was done for dual carbon containing sugar glucose and maltose with concentration ratio 1:1. Cultivation with medium containing maltose was done for comparison between single carbon and dual carbon. For dual carbon cultivation, highest biomass form at day 7 th at 0.44g/ml while specific enzyme activity reached 10.54 U/g at day 8 th . For single substrate, highest biomass production was recorded at day 9 th with 0.41g/ml.while specific enzyme activity at day 7 th at 4.02 U/g. Index Terms—cellobiohydrolase (CBH), dual carbon cultivation, recombinant Aspergillus niger. static surface liquid culture. (SSCL)","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123534964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Remote Homology Detection by Combining Profile-based Protein Representation with Local Alignment Kernel 基于蛋白质谱表示与局部比对核相结合的蛋白质远程同源性检测

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.1.17-22

Bin Liu, Xiaolong Wang, Ruifeng Xu, Buzhou Tang

引用次数: 2

Vasa Vasorum Angiogenesis through Increased Levels of H2O2, HIF-1α, NF-κB and INOS: In Vivo Study of Atherosclerosis 通过H2O2、HIF-1α、NF-κB和INOS水平升高的血管新生:动脉粥样硬化的体内研究

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/jomb.4.5.342-349

T. A. Wihastuti, D. Sargowo, Setyawati Soeharto, T. Heriansyah, Grace R. Widyasih

引用次数: 3

Ammoniacal Nitrogen Removal in Semiconductor Wastewater by Sequence Batch Reactor Using Bacteria Inoculum from WormTea 利用虫茶菌接种物在序批式反应器中去除半导体废水中的氨态氮

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.4.241-244

Farehah Z. A. Nur, I. Norli, Norfariha M. N Siti, A. I. Siti, R. Renuka

引用次数: 4

Incidence of Breast Cancer in a Primary Hospital in Relation to ABO Blood Groups System 某基层医院乳腺癌发病率与ABO血型系统的关系

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.1.74-77

S. Abobaker, M. Kamil

引用次数: 2

Describing Wing Shape among Culex Quinquefasciatus Say (Diptera: Culicidae) Detected Positive and Negative for Filaria Using Relative Warp Analysis 用相对翘曲分析方法描述致倦库蚊翅型(双翅目:库蚊科)丝虫检测阳性和阴性

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.1.50-54

J. P. Sendaydiego, J. Gorospe, A. Torres, R. Amparado, C. Demayo

{"title":"Describing Wing Shape among Culex Quinquefasciatus Say (Diptera: Culicidae) Detected Positive and Negative for Filaria Using Relative Warp Analysis","authors":"J. P. Sendaydiego, J. Gorospe, A. Torres, R. Amparado, C. Demayo","doi":"10.12720/JOMB.3.1.50-54","DOIUrl":"https://doi.org/10.12720/JOMB.3.1.50-54","url":null,"abstract":"This study examined populations of Culex quinquefasciatus in filariasis-endemic communities of Misamis Oriental, Philippines. C. Quinquefasciatus, has been of interest to public health since it was identified as a vector of filariasis in the Philippines in 1995. In surveillance and control programs, problems in identification is encountered as it requires some specialized skill and a well preserved set of specimen which is sometimes difficult to acquire. It is for these reasons that alternative method of identification and discrimination of Culex individuals has been desired to resolve problems related with vector species diversification and identification. In this study, geometric morphometric approach was used to test the hypothesis that there exists some variation in the wing shape pattern of C. Quinquefasciatus identified as positive and negative of filaria based on the landmarks' position on the wings. To illustrate ordination of the shapes' consensus, the mean shape of the two populations was measured by a relative warp ordination plot using the software tpsRelw version 1.46. Results of the relative warp analysis showed significant variation between the two populations. The four extracted significant relative warps account for a total of 68.54% variation in wing geometry pattern. Variations were observed in the wing apex and base. These variations may have genetic basis or maybe mere reflections of phenotypic plasticity brought about by the changing environmental conditions. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127842427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2