Protein Remote Homology Detection by Combining Profile-based Protein Representation with Local Alignment Kernel

Abstract

Protein remote homology detection has attracted a great deal of interest as it is one of the most important problems in bioinformatics. Profile-based methods recently achieve the state-of-the-art performance. A key step to improve the performance of these methods is to find a suitable approach to use the evolutionary information in the profiles. In this study, we propose the profile-based protein representation to extract the evolutionary information from frequency profiles. In this approach, the frequency profiles calculated from the multiple sequence alignments outputted by PSI-BLAST are converted into several profile-based proteins and then the local alignment kernel (LA) is combined with these profile-based proteins for the prediction. Our experiments on a well-known benchmark show that the proposed approach can significantly improve the predictive performance.