Journal of Reproductive Healthcare and Medicine最新文献

{"title":"Sperm microencapsulation in bovine: An overview","authors":"N. Paul, T. Talluri, Arumugam Kumaresan","doi":"10.25259/jrhm_14_2024","DOIUrl":"https://doi.org/10.25259/jrhm_14_2024","url":null,"abstract":"Artificial insemination is so far the most successful and widely adopted assisted reproductive technique for genetic improvement in bovines. Despite their widespread adoption, the field conception rate using frozen semen straws remain low. Prediction of ovulation time, the short lifespan of frozen-thawed sperm, and retrograde backflow of semen following insemination result in fewer sperm available in the sperm reservoir in the oviduct and thus lessen the chances of fertilization. To tackle this, the idea of sustained release of spermatozoa inside the oviduct to maximize the odds of fertilizing the descendent ova is generated. Cell microencapsulation is a strategy that allows the implantation of cells, keeping the cells isolated from the host immune response by semipermeable membrane permitting the selective diffusion of gases, nutrients, and therapeutics but not of host immune cells. At present, few studies have been conducted where spermatozoa were encapsulated in a polymer shell/bead and successfully cryopreserved. The frozen-thawed encapsulated spermatozoa had shown comparable results in terms of in vitro functional assessment as well as in achieving pregnancy as compared to conventional semen dose. The purity of encapsulating material is very crucial to nullify maternal immune response as well as to achieve higher biocompatibility. In this manuscript, an overview of sperm encapsulation has been compiled, with more stress on types of encapsulating materials, their characterization, purity, and prospects of this method to come up as a robust method for achieving higher success following artificial insemination.","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"11 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141920617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control of sperm quality in the epididymis by disintegration and removal – A transmission electron microscopy study of abnormal sperm of aflatoxin-treated rat","authors":"K. Faisal, M. A. Akbarsha","doi":"10.25259/jrhm_23_2023","DOIUrl":"https://doi.org/10.25259/jrhm_23_2023","url":null,"abstract":"\u0000\u0000The mammalian epididymis is a dynamic organ endowed with diverse roles, including sperm quality control, which appears to occur during the epididymal processing and storage to prevent the misshapen spermatozoa from being present in the ejaculate in large numbers. This quality control mechanism is capable of identifying and removing dead/defective spermatozoa. In this respect, the role of a dense matrix arising from epididymal secretory aposomes/epididymosomes and associated disintegration/dissolution of dead/defective spermatozoa has been reported. This study was conceived to find if the mechanistic details of the disintegration of the parts of the spermatozoa along the length encompass the same or different patterns.\u0000\u0000\u0000\u0000We used photomicrographs from the experimental rat model induced into defective/unviable spermatozoa by aflatoxin B1 treatment.\u0000\u0000\u0000\u0000A meticulous screening of the matrix-embedded spermatozoa reflected different courses of disintegration of the major parts, namely, head, mid-piece, and flagellum. The disintegration of the head began with swelling of the acrosome and its lysis, followed by the gradual disintegration of the nucleus. In the mid-piece, the plasma membrane underwent disintegration first, and this was followed by disorganization and disintegration of the mitochondrial sheath and, subsequently, the outer dense fibers (ODFs). In the flagellum, neither the plasma membrane nor the fibrous sheath (FS) indicated any trace of disintegration initially. Rather, the FS and the plasma membrane were lifted off from the ODF-axoneme complex and, subsequently, with the FS remaining intact, the ODFs and the axonemal doublets on one side disintegrated, followed by disintegration of the remaining ODFs and the axonemal doublets.\u0000\u0000\u0000\u0000In the disintegration process, the spermatozoa’s head, mid-piece, and flagellum follow different courses, reflecting on the respective structural compositions.\u0000","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"15 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autologous platelet-rich plasma as a potential new approach in the endometrial response during in vitro fertilization cycle: A pilot study","authors":"Rita Bakshi, Upendra Kumar, Biva Prasad, S. Gautam, Lipi Singh","doi":"10.25259/jrhm_5_2024","DOIUrl":"https://doi.org/10.25259/jrhm_5_2024","url":null,"abstract":"\u0000\u0000Infertility is a global health concern, affecting ~13% of couples. Despite assisted reproductive technology (ART) attempts, implantation failure occurs due to inadequate growth of the endometrium. Increasing endometrial thickness (ET) can be increased to improve the rate of pregnancy; implantation necessitates a minimum thickness of 7 mm. Platelet-rich plasma (PRP) is a recommended treatment approach for endometrium and ovarian infertility. This approach minimizes the potential of immunogenic reactions and disease transmission because PRP comes from an autologous source. The purpose of this study was to evaluate the efficacy of PRP intrauterine infusions during the in vitro fertilization (IVF) cycle in patients with thin endometrium.\u0000\u0000\u0000\u0000Ten patients with primary infertility (age: 28–40 years) were chosen for intrauterine PRP infusion at the RiSSA IVF Center, Delhi. This study was carried out between June 2020 and January 2022 over a span of 18-month period. Intrauterine infusion of PRP was an additional procedure to hormone replacement therapy (HRT) treatment cycle. PRP was prepared by centrifugation process from autologous blood. On the 10th day of HRT cycle, 2 mL of PRP was infused into the uterine cavity. In each cycle, PRP infusion was administered 1–3 times if there was no increase in ET 72 hours later. Out of ten patients, four patients received a single infusion, two patients received two infusions, and four patients received three infusions. The embryos were transferred when the ET reached ≥7 mm. ET was measured at the uterine longitudinal axis at the thickest point. To determine ET, three measurements were made, and the average of those measures was noted. The primary outcome measure was ET, determined by transvaginal sonography, and the secondary outcome measure was clinical pregnancy following embryo transfer.\u0000\u0000\u0000\u0000The mean increase in ET was 1.8–2.25 mm. In 8 (80%) patients, there was an increase of 7–7.5 mm in ET. ET thickness did not improve in 2 patients (20%), and it was <6 mm after three infusions of PRP. Further, of the eight patients who had embryo transfer and became pregnant, six patients had a clinical pregnancy with visible cardiac activity at 6 weeks, while two patients had a missed abortion in the first trimester. Six patients had a successful delivery.\u0000\u0000\u0000\u0000This study revealed that PRP could support endometrial growth, improving pregnancy outcomes in patients who have thin endometrium. PRP is a novel treatment option for endometrial thinning and poor response to IVF. The findings of the current pilot study support the need for large-scale, randomized, controlled trials in this field.\u0000","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"36 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140979747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics-based cutting-edge technologies for identifying predictive biomarkers to measure the impact of air borne particulate matter exposure on male reproductive health","authors":"Roshani Kumari, Prasan Kaur, Sandeep Kumar Verma, Pooja Ratre, P. K. Mishra","doi":"10.25259/jrhm_25_2023","DOIUrl":"https://doi.org/10.25259/jrhm_25_2023","url":null,"abstract":"The reproductive lifespan of an individual is a critical determinant of their health, population dynamics, and aging. Research has established a clear association between environmental air pollution, particulate matter (PM), and reproductive health. Recent studies have focused on the impact of air pollution on male reproductive health. Chronic or acute exposure to airborne PM0.1, PM2.5, and PM10 has been found to trigger mitochondrial oxidative stress, double-strand DNA breaks, epigenetic modifications, and endocrine disruption in male reproductive tract functions. Consequently, identifying and validating PM-associated predictive biomarkers, including genes, transcripts, epimutations, proteins, and metabolites, hold promise for improving male reproductive efficiency. Omics-based techniques, such as next-generation sequencing, comparative genomic hybridization, genome-wide association studies, single-cell RNA sequencing, microarray analysis, mass spectroscopy, 2D gel electrophoresis, Raman spectroscopy, near-infrared spectroscopy, and nuclear magnetic resonance, have provided crucial insights into the pathological mechanisms underlying air pollution-related male reproductive health issues. This article presents a comprehensive assessment of existing evidence in this field, offering a methodical examination of findings that hold immense potential for addressing the adverse effects of air pollution on male reproductive health.","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcome of intrauterine insemination in couples with unexplained or male factor infertility at National Referral Hospital of Bhutan","authors":"Gyem Dorji, Kanokwan Wetasin, Nidup Gyeltshen, Minjur Dorji","doi":"10.25259/jrhm_24_2023","DOIUrl":"https://doi.org/10.25259/jrhm_24_2023","url":null,"abstract":"\u0000\u0000Jigme Dorji Wangchuck National Referral Hospital (JDWNRH) is the only facility offering intrauterine insemination (IUI) services in the country. In this study, we aim to study the success rate of IUI for the year 2022.\u0000\u0000\u0000\u0000This retrospective study utilized secondary data extracted from the medical records of a cohort of 57 patients who underwent IUI procedures at the infertility clinic between January and December 2022. Ethical clearance and site approval were obtained from the Institutional Review Board at Khesar Gyalpo University of Medical Sciences of Bhutan and JDWNRH, respectively. The Statistical Package for the Social Sciences version 26.0 by IBM Corp in Armonk, NY, was used to calculate frequencies, percentages and create statistical diagrams.\u0000\u0000\u0000\u0000In the year 2022, the overall success rate of IUI was 5.3%. A majority of infertile men, representing 78.9%, fell within the age range of 30–39 years, and oligozoospermia emerged as the most common semen abnormality, affecting 45.6% of the infertile men in this study.\u0000\u0000\u0000\u0000Our study revealed that the IUI success rate in the country was 5.3%. This success rate of IUI in the country was found to be low compared to the success rate in neighboring countries. This is important information for patients to make informed decisions, aids fertility specialists in devising treatment plans, directs the allocation of resources, drives research and development initiatives, and informs policy and strategic adjustments at the national level.\u0000","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"29 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140411746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of fibroblast growth factor 8 in different cancers","authors":"Swati Ranjan, A. Saini, S. Yadav","doi":"10.25259/jrhm_10_2023","DOIUrl":"https://doi.org/10.25259/jrhm_10_2023","url":null,"abstract":"Fibroblast growth factor 8 (FGF8), a secretory protein of the FGF family, is highly expressed during early developmental stages. The early-stage expression of FGF8 and its isoforms is crucial for the normal development of organisms, while their expressions in adulthood are limited to the steroid hormone-targeting tissues. Interestingly, differential expression of FGF8 has been associated with the progression of various cancer types including breast, prostate, and ovarian cancers. Specifically, in gynecological cancers, the expression of FGF8 is regulated by steroid hormones. FGF8 isoforms, that is, FGF8a, FGF8b, FGF8e, and FGF8f act through different fibroblast growth factor receptors in different cancers through three main signaling pathways – MAP/RAS kinase, AKT/PI3, and PCLγ. This short review article discusses the structure and functions of FGF-8, along with its role in different cancers.","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129192914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bulk stiffness and recovery time – A measure of development of mouse embryos","authors":"K. D. Patil, Praveen Kumar, Kishor K. S. Bharadwaj, S. Hegde, Sai Abhishek Dharamkar, Binal Gupta, T. J. R. Babu, D. B. S. Bhargav","doi":"10.25259/jrhm_26_2022","DOIUrl":"https://doi.org/10.25259/jrhm_26_2022","url":null,"abstract":"\u0000\u0000Understanding the onset of embryonic arrest by studying the morphology of the embryos at different stages of their development is a relatively well-understood subject. The qualitative nature of the study makes it difficult to determine and quantify the causes behind the arrest. For successful embryonic development, it is essential to know, non-destructively, and the developmental viability of the embryos at a particular stage.\u0000\u0000\u0000\u0000We investigate the bulk stiffness and recovery time as the viability quantifiers for grading or classifying embryos at the two-cell stage or after the formation of the first cleavage. A simple cantilever beam-based experimental procedure is established to investigate two-cell mouse embryos in two distinct orientations.\u0000\u0000\u0000\u0000Bulk stiffness measurement varies with the embryo’s orientation and deformation. A good quality two-cell embryo shows a significant decrease in stiffness ratio with the deformation. Also, the embryo’s recovery time after releasing deformation may indicate a quality-dependent correlation.\u0000\u0000\u0000\u0000Our study concludes that the bulk stiffness measurements in the two principal orientations, their ratio, and recovery time correlate with the underlying morphology of two-cell stage mouse embryos.\u0000","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123948997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An N-terminal peptide of Tar DNA binding Protein 43 lacking nuclear localization signal translocates to the nucleus of GC-1 spermatogonial cells","authors":"D. S. Varghese, G. Vysakh, Pradeep G. Kumar","doi":"10.25259/jrhm_10_2022","DOIUrl":"https://doi.org/10.25259/jrhm_10_2022","url":null,"abstract":"\u0000\u0000TAR DNA-binding protein of 43 kDa (TDP-43) is an RNA/DNA binding protein expressed in the brain and the testis. Mutations in TDP-43 lead to mislocalization and cytoplasmic aggregation of this protein causing neurodegenerative diseases such as amyotrophic lateral sclerosis and frontotemporal dementia. TDP-43 has also been implicated in maintaining spermatogenesis. While homodimerization of TDP-43 is critical for its physiological functions, higher-order aggregation of this protein impairs its functions. This study was aimed to map the critical amino acids of the N-terminus of this protein in mediating its homodimerization.\u0000\u0000\u0000\u0000We generated deletion constructs of Tdp-43 containing NRRM1 domain alone (TDP-43∆3-183) and N-terminal peptide of TDP-43 which lacks the nuclear localization signal (NLS) (TDP-43∆1-50) with fluorescent reporters having non-overlapping emission properties. These constructs were co-transfected into a mouse spermatogonial cell line to examine their dimerization and nuclear translocation capabilities in vitro.\u0000\u0000\u0000\u0000We found that TDP-43∆3-183 alone was not capable of homodimerization. On the other hand, TDP-43∆1-50 when co-transfected into GC1-spg cells along with full length TDP-43 translocated to the nucleus oligomerized with the latter and translocated to the nucleus, indicating the importance of amino acids 1-50 of TDP-43 in dimerization.\u0000\u0000\u0000\u0000The N-terminal segment of TDP-43 spanning amino acids 1-50 is responsible for dimerization, while that spanning amino acids 51-183 directs it to the nucleus.The physiological and pathological implications of this finding need to be examined.\u0000","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130303144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 pandemic on maternal and neonatal outcomes: A narrative review and evidence from the PregCovid registry","authors":"Sarika Zala, Hrishikesh Munshi, N. Mahajan, Suchitra Surve, R. Gajbhiye","doi":"10.25259/jrhm_11_2022","DOIUrl":"https://doi.org/10.25259/jrhm_11_2022","url":null,"abstract":"More than 533 million Coronavirus Disease 2019 (COVID-19) cases and associated 6 million fatalities were reported globally whereas 43 million cases and 0.5 million deaths in India were reported till June 2022. Maharashtra state alone reported about one-third of the total cases of COVID-19 in India in the early period of COVID-19 pandemic. The lack of epidemiological, demographic, and impact data of COVID-19 on pregnant women and newborns, advocated the need for recording and documenting population specific data for clinical management and policy decisions in India. The PregCovid registry was launched in April 2020 by the Indian Council of Medical Research-National Institute for Research in Reproductive and Child Health, Mumbai, in collaboration with the Medical Education and Drugs Department, Government of Maharashtra, and Topiwala National Medical College and Bai Yamunabai Laxman Nair Charitable Hospital to gain insights into clinical and socio-epidemiological characteristics of pregnant women with lab confirmed COVID-19 and assess its impact on maternal and neonatal outcomes. The registry has collected data of 8428 pregnant and postpartum women with COVID-19 from 19 participating centers across Maharashtra in a near real-time manner. The registry first reported that the delta variant (B.1.617.2) dominant second wave was more lethal (case fatality rate 5.7%) to pregnant and postpartum women than the alpha (0.7%) dominant wave of COVID-19 in India. There was also a higher incidence of moderate to severe cases, intensive care admissions and maternal complications including pre-eclampsia, low birth weight and preterm deliveries during the delta wave. The omicron dominant third wave of the COVID-19 pandemic exhibited a higher transmission rate compared to the previous two waves, causing a surge in cases but minimally impacting adverse outcomes. The registry further analyzed and reported the impact of COVID-19 on pregnant and postpartum women with comorbidities, coinfections, twin pregnancies, and neonatal outcomes, while providing crucial policy inputs to improve maternal and newborn health during the pandemic. The registry model can be replicated at tertiary care hospitals across India to understand various maternal-neonatal outcomes. The evidence generated from PregCovid registry was useful for improved clinical management and also contributed to a policy decision on COVID-19 vaccination in pregnant women in India. The registry envisions a collaboration with similar regional, national and international registries to form an international consortium for data sharing and reporting to promote global policy level interventions and advocates a sustainable and collective response to improve the COVID-19 global vaccination coverage.","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126192963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the pharmacological potential of Indian spices in polycystic ovarian syndrome","authors":"Neelam Sharma, Vishal Kumar, Urvashi Langeh, Charan Singh, Arti Singh","doi":"10.25259/jrhm_21_2021","DOIUrl":"https://doi.org/10.25259/jrhm_21_2021","url":null,"abstract":"\u0000\u0000India has a distinct position in history for using spices from cooking to prevent and cure a variety of illnesses. Numerous studies have been conducted to evaluate the potential of these spices for a variety of conditions such as diabetes, metabolic syndrome, and polycystic ovarian syndrome (PCOS). PCOS is a condition in which women of reproductive age experience common health difficulties such as genetic, metabolic, and endocrine abnormalities. Notably, this may be distinguished by symptoms such as hirsutism, acne, and obesity.\u0000\u0000\u0000\u0000In this review, we looked at numerous Indian spices that may help to manage PCOS and its symptoms.\u0000\u0000\u0000\u0000All data were gathered from the literature using several databases such as PubMed and Europe PMC, publishers such as Springer, Wiley, Elsevier, and Taylor and Francis, and academic libraries such as Google scholar and Sci-Hub.\u0000\u0000\u0000\u0000This study focuses on natural spices and their chemical constituents that have varied therapeutic effects such as anti-inflammatory, anti-oxidant, anti-androgenic, anti-estrogenic, anti-thrombolytic, and anti-diabetic for the treatment of PCOS in women.\u0000\u0000\u0000\u0000In this review, we looked at the effects of several Indian spices on the management of PCOS.\u0000","PeriodicalId":434467,"journal":{"name":"Journal of Reproductive Healthcare and Medicine","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133543880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}