Veterinary Biomedical and Clinical Journal最新文献

{"title":"Prevalence and Predisposing factors of Subclinical Mastitis in Dairy Cows in Bakir, Sukomulyo Village, Batu Regency","authors":"M. Fatmawati, A. Setianingrum, A. Haskito, F. Dameanti","doi":"10.21776/UB.VETBIOCLINJ.2019.001.02.5","DOIUrl":"https://doi.org/10.21776/UB.VETBIOCLINJ.2019.001.02.5","url":null,"abstract":"Udder inflammation in dairy cows known as mastitis is still a major problem in dairy industry. Economic loss caused by mastitis burden on farmers due to decrease on milk yield and decrease on the milk quality. Most of traditional farmers recognize clinical mastitis as the symptoms was obvious, while subclinical mastitis were not easy to detect and can be potential source of infection for the herd. Detection on subclinical mastitis were using indirect test california mastitis test (CMT) and direct test with Somatic Cell Count (SCC). This research was a cross sectional study using udder quarters milk sample from traditional dairy farms in Bakir, Sukomulyo village, Batu regency. Field test conduct uding CMT and SCC counting in laboratorium. Data collection using Questionnaire were conduct to asses predisposing variables on farmers. Result shows prevalence of subclinical mastitis based on SCC was 60,22% and based on CMT 18,20% of quarter sampels. Predisposing factors that have effect on mastitis were the sanitation of the cow and the farm before and after the milking process.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133834786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE STUDY OF ORGANOPHOSPHATE (DIAZINON) TOXICITY TOWARD LIVER HISTOPATHOLOGY AND MALONDIALDEHYDE (MDA) SERUM LEVELS ON RATS (Rattus norvegicus)","authors":"D. A. Pratama, Zulfa Aulia, A. Aulanni’am, F. S. Permata","doi":"10.21776/UB.VETBIOCLINJ.2019.001.02.3","DOIUrl":"https://doi.org/10.21776/UB.VETBIOCLINJ.2019.001.02.3","url":null,"abstract":"Diazinon is an insecticide that has a higher toxicity than other insecticides. Normally, insecticides are detoxified by liver, but this process produced free radicals which causes cell damage. Free radicals also increase lipid peroxidation which directly increase malondialdehyde levels. This research was aimed to determine the influence of diazinon toxicity to the liver histopathology and levels of malondialdehyde (MDA) in the serum of rats (Rattus norvegicus). The rats were divided into 4 groups which consist of negative control group and three experimental groups which were given diazinon 20 mg/kgBW (P1), 40 mg/ kgBW (P2), and 60 mg/ kgBW (P3). The rats were given diazinon for 8 weeks orally. The parameters used in this research was liver histopathology with hematoxilin eosin stain and Malondialdehyde levels with Thiobarbituric test. Liver histopathology was analyzed in descriptive qualitative and the level of MDA was analyzed quantitatively using ANOVA and Tukey's exact test with α = 0.05. The result of this research showed the influence of organophosphate (diazinon) on liver histolopathology was shown by inflammatory cells infiltration in hepatic parenchyma, sinusoidal congestion, and cytoplasmic vacuolation of the hepatocytes. Statistical analysis proved that the diazinon was able to increase the level of MDA serum significantly (p<0,05) up to 71% in experimental group P1 (dose 20 mg/kgBW), which the increase level of MDA serum depend on dose of diazinon. This research concluded that diazinon is one of the organophosphate pesticide that toxic based on liver histopathology and MDA serum levels.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"109 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131389406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Classical Swine Fever in Papua, Indonesia: Demography of Pig Farming and Vaccination Rate","authors":"Widi Nugroho","doi":"10.21776/ub.vetbioclinj.2019.001.02.6","DOIUrl":"https://doi.org/10.21776/ub.vetbioclinj.2019.001.02.6","url":null,"abstract":"Classical Swine Fever (CSF) is an important disease in the pig industry. In the end of 2003, CSF caused a mortality outbreak involved thousands of pigs in Timika, Papua. Control of CSF in Timika has been through vaccination program using strain C vaccine. However, demographic data of pig farming in Timika was unavailable, made the assessment of the vaccination rate impossible. The research aimed to comprehend the demography of pig farming dan the rate of vaccination impossible. The research aimed to comprehend the demography of pig farming and the rate of vaccination against CSF in five districts in Timika. The study was conducted in 25 of 40 villages in five districts. Data of the demography was collected from all families in sample villages. Data of vaccine delivery during 2018 was collected in village level. Data of demography and vaccination rate was analysed descriptively, correlations between vaccination rate and the pig population, the number of farms the size of farmsat village level were analysed at 0.05, using Excel 2010 (Microsoft Excel, USA). Results showed that pig population was 16.846 heads, farmers were 2.560 families, human population was 133.186 persons, farm size was 6.6 heads per farm, ratio of pigs to humans was 0.13 pigs per-person. Only four villages obtained vaccination rates of above 70% of their respective pig populations. In seven other villages vaccination covered less than 30% of pig populations, while other 14 villages did not obtain CSF vaccine delivery. No correlation appeared between thevaccination rate in a village with the size of its pig population, the number off arms in it or the average size of farm in it (P>0.05). This study could become a reference point for designing CSF vaccination program in Timika in the future.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":" 15","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113950167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Plant Leaves Variants from The Philippines on Infective Oesophagustonum dentatum Larvae","authors":"M. A. G. Beltran, A. Robertson, R. Martin","doi":"10.21776/ub.vetbioclinj.2019.001.01.2","DOIUrl":"https://doi.org/10.21776/ub.vetbioclinj.2019.001.01.2","url":null,"abstract":"Nine aqueous extracts of plant leaves from the Philippines were evaluated against Oesaphagustomum dentatum sensitive (ODSEns) larvae for their anthelmintic effects. The plant species evaluated were Ananas comosus, Chrysophyllum cainito,Gliricidia sepium, Annona squamosa, Moringa oleifera, Tinospora rumphii Boerl,Azadirachta indica, Trichantera gigantea and Leuacaena leucocephala. The leaves of the plants gathered from Camiling, Tarlac, Philippines, were dried under room temperature for 30 days and then ground. Aqueous extracts of these plant leaves were prepared using migration buffer. In vitro migration and inhibition assays were used to evaluate the anthelmintic activity against exsheathed third stage larvae of Oesophagustomum dentatum. The experiments were put in 24-well plates at room temperature with four replications per treatment using different concentrations of 1, 2.5, 5 and 10 mg/ml. About 120 larvae per well were deposited on a larval migration apparatus consisting of 20 μm nylon mesh filters and incubated 20 hours under different treatments and another 2 hours to allow the migration of active motile larvae. The evaluation criteria were based on the average numbers of inhibited larvae under the different treatments. The percentage inhibition were statistically analyzed using Two-Way ANOVA (P=≤.0001) of the Graph Pad Prizm software (V5 San Diego, Ca). The most effective leaves with their highest inhibition level were from Ananas comosus (pineapple, 86.50%), Gliricidia sepium (madre de cacao, 84.50%), Chrysophyllum cainito (star apple, 83.75%), Moringa oleifera (horseradish,58.75%), Annona squamosa (sweet sop, 56.00%), Tinospora rumphii Boerl ( Makabuhay, 55.75%), and Azadirachta indica (Neem, 51.50%), whereby extremely significant (P˂0.001) effect against O. dentatum larvae. Two leaves extracts, from Trichantera gigantea and Leuacaena leucocephala have no significant effect on O. dentatum larvae. The larval migration assay allows initial studies on the anthelmintic effects of the herbs.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124739610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Staphylococcus aureus Biofilm from Subclinical Mastitis Milk","authors":"M. A. Lesmana, Dahliatul Qosimah, Sri Murwani","doi":"10.21776/ub.vetbioclinj.2019.001.01.3","DOIUrl":"https://doi.org/10.21776/ub.vetbioclinj.2019.001.01.3","url":null,"abstract":"One of S.aureus's virulence factors is biofilm formation. When biofilms are formed, the bacteria will undergo phenotypic changes that require higher concentrations of antibiotics to inhibit proliferation. Phenotypic changes will lead to increase the production of extracellular matrix and multilayered colonies as well as decrease of metabolic rates, multiplication and polymicrobial colonization resulting in recurrent infection in the host and difficulty being treated with antibiotics due to resistance. The aim of this research was to know the formation of bacterial biofim by slime and quantitative by microplate titer method. The research method was qualitative descriptive using 27 samples of Staphylococcus aureus with characterized from mastitis infected milk. The bacteria were grown on CRA (Congo Red Agar) media to observe the slime biofilm through bacteria black colony followed by MicrotiterPlate method with 570nm wave lenght. The results showed that 27 samples of Staphylococcus aureus which positive to form slime biofilm were 10 samples and continued to microtiter plate showed 3 positive samples of biofilm. The conclusions of this study, Staphylococcus aureus in subclinical mastitis milk samples were positive to form biofilms.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131106417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity of Goat Milk Yogurt Casein on Dioxin Intoxicated Rat Liver Function","authors":"A. Setianingrum, M. Padaga","doi":"10.21776/ub.vetbioclinj.2019.001.01.4","DOIUrl":"https://doi.org/10.21776/ub.vetbioclinj.2019.001.01.4","url":null,"abstract":"Dioxin is toxic contaminant which persistent in the environment. This compound bio-accumulate through food chain and generate adverse effect in human health. Oxidative stress due to dioxin exposure can interfere metabolism process and leads to the development of hepatotoxicity.The aim of this study was to asses the role of casein derived from goat milk yogurt in dioxin-intoxicated rat based on liver function parameters. Substances used were: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) diluted in corn oil and casein from goat milk yogurt. The study included 24 Wistar rats divided into 6 groups: (1) control group, (2) placebo group which given casein 600 mg/kg BW, (3) TCDD group given with TCDD 100 ng/kg BW, (4) group P1 given casein 300 mg/kg BW and TCDD, (5) group P2 given casein 600 mg/kg BW and TCDD, (6) group P3 given casein 900 mg/kg BW and TCDD. The respective dose were given orally for 21 days. Blood serum was collected for transaminases (ALT, AST) and γ-glutamyl transferase (GGT) analysis. Based on serum transaminases activity, there were no significant change on AST and ALT level. Casein yogurt supplementation has significant effect on serum GGT level (p<0,05) and optimum dose was 600 mg/kg BW/day","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"170 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131244831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Triponyl Sulphate on Fetuses Development and Placental Abnormalities in Inducing Preeclampsia of Rattus norvegicus animal model","authors":"W. Purwatiningsih, D. E. Aryani, D. Vidiastuti, Y. Oktanella, A. Firmawati","doi":"10.21776/UB.VETBIOCLINJ.2019.001.01.6","DOIUrl":"https://doi.org/10.21776/UB.VETBIOCLINJ.2019.001.01.6","url":null,"abstract":"Preeclampsia is one of the obstetrical problems that can cause maternal and fetal morbidity and mortality. Preeclampsia causes the fetus born prematurely and low fetal weight. This is caused by high blood pressure which causes decrease of blood delivery to the placenta, so the supply of oxygen and food to the fetus decreases. As a result, fetal development inhibits and trigger born prematurely. More fatal, this disease cause the release of placental tissue from the uterus prematurely. The aim of this study was to determine the effect of administration of triponyl sulfate as induction of increased blood pressure in preeclampsia animal models, fetal development with alizarin red staining and placental abnormalities. The experimental animals were rats Rattus norvegicus mated with male rats monomating , 4 months old and 250-300 grams body weigh. Pregnant female rats were induced by triponyl sulfate 70 mg / kg BW (k +) and without induced by triponyl sulfate (k-). The results of the study showed that there were formation of the sternal bone in k- and malformation of the sternum bone at k +. Placental abnormalities occured in k +, it could be seen in the presence of ghos villi in blood vessel abnormalities in the preeclampsia placenta caused by there was no invation of trophoblast cells in the whole or partial spiral arteries and the mean of blood pressure increased.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"62-63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131019085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Hydroureter in Rat After Medial and Distal Unilateral Ureteral Obstruction Using Ultrasound Frequency 10 MHz","authors":"Nofan Rickyawan, Dinar Arifianto, Dhirgo Adji","doi":"10.21776/UB.VETBIOCLINJ.2019.001.01.5","DOIUrl":"https://doi.org/10.21776/UB.VETBIOCLINJ.2019.001.01.5","url":null,"abstract":"Unilateral ureteral obstruction can occur in all animals and it is located proximal, medial and distal. Obstruction causes abnormalities in ipsilateral ureters in the form of hydrouterers. The aim of this study was to detect hydroureters in post-unilateral medial and distal ureteric rats using a 10 MHz ultrasonography frequency. Thirty-six female Sprague Dawley rats aged 3 months were divided into 3 treatment groups. Each group consisted of 12 individuals. Group I as a control received laparotomy treatment. Group II laparotomy with medial right-hand ureteral ligation and group III with right distal ureteral ligation. Three rats taken randomly from each group at weeks 1, 2, 3 and 4 post-ligation is then performed ultrasonography. The results of ultrasonography were analyzed descriptively, comparatively but ureteral diameter were analyzed using statistical of 3x4 factorial patterns. Ultrasound examination showed that ligation in one ureter caused rat undergoing unilateral ureteral obstruction and hidroureter. The diameter of the ureter with a hydroureter has increased from the 1st to 4th week. The increase occurs because the urine in the lumen cannot be excreted. The contralateral ureter remains normal and cannot be observed. The conclusion of the study is that hydroureter in rats can be detected using the frequency of 10 MHz ultrasonography which is characterized by anekoic tubular formation posterior to the right kidney. The unilateral ureteral obstruction has no effect on the contralateral ureter because it is still able to compensate for the performance of the ipsilateral ureter that has a hydroureter. The difference in the location of ligation and time of examination affect the increase in ipsilateral ureteral diameter.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"3271 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127487994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potency of Pluchea indica Less as Preventive Effort to Lead (Pb) Pertubation on Rats (Rattus norvegicus) Organs","authors":"A. Aulanni’am, Andri Julianto, Made Artari Dewi, Tri Cahyo Dirgahariyawan, C. Mahdi, D. Wuragil, Herawati Herawati","doi":"10.21776/ub.vetbioclinj.2019.001.01.1","DOIUrl":"https://doi.org/10.21776/ub.vetbioclinj.2019.001.01.1","url":null,"abstract":"Lead (Pb) is one of the heavy m metals polluting the environment which is harmful to t the organism. Pb accumulation triggers free radic cals and disrupts the body's metabolism. Pluche indic ca leaf extract is reported contain flavonoids whiich act as antioxidants and can counteract free radiccal attacks. The purpose of this study was to de etermine the role of Pluchea indica leaf extract preve entive therapy in counteracting free radicals due too exposure to Pb in rats (Rattus norvegicus). Wistar mmale rats aged of 8-10 weeks with a body weight of about 200-250 grams. Rats were divided into 5 tr reatment groups, were group A as negative contr rol, group B as positive control induced by lead (Pb)) 10 mg/rat/day, groups C,D,E given with ethano olic extract of Pluche indica leavesof 200 mg/kgBW, , 400 mg/kgBW, and 600 mg/kgBW and lead (Pb b) exposure of 10mg/ rat/day. Kidney, liver and jejunaal histopathology using HE staining was observed microscopically. The results showed that the administtration of Pluche indica leaves extract could preve ent tissue damage to the kidney, liver and jejunum in rats exposured to Pb. Dose of 600 mg/kgBW show wed histopathological improvement in kidney, liver annd jejunum. The greater the dose, the better prev ention of tissue damage as indicated by tissue condition resembling those of control rats. The conclusion of this study, the Pluchea indica leaf extract has the potential to prevent organ damaged exposed to lead.","PeriodicalId":432813,"journal":{"name":"Veterinary Biomedical and Clinical Journal","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125605165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}