The Effects of Plant Leaves Variants from The Philippines on Infective Oesophagustonum dentatum Larvae

M. A. G. Beltran, A. Robertson, R. Martin
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引用次数: 1

Abstract

Nine aqueous extracts of plant leaves from the Philippines were evaluated against Oesaphagustomum dentatum sensitive (ODSEns) larvae for their anthelmintic effects. The plant species evaluated were Ananas comosus, Chrysophyllum cainito,Gliricidia sepium, Annona squamosa, Moringa oleifera, Tinospora rumphii Boerl,Azadirachta indica, Trichantera gigantea and Leuacaena leucocephala. The leaves of the plants gathered from Camiling, Tarlac, Philippines, were dried under room temperature for 30 days and then ground. Aqueous extracts of these plant leaves were prepared using migration buffer. In vitro migration and inhibition assays were used to evaluate the anthelmintic activity against exsheathed third stage larvae of Oesophagustomum dentatum. The experiments were put in 24-well plates at room temperature with four replications per treatment using different concentrations of 1, 2.5, 5 and 10 mg/ml. About 120 larvae per well were deposited on a larval migration apparatus consisting of 20 μm nylon mesh filters and incubated 20 hours under different treatments and another 2 hours to allow the migration of active motile larvae. The evaluation criteria were based on the average numbers of inhibited larvae under the different treatments. The percentage inhibition were statistically analyzed using Two-Way ANOVA (P=≤.0001) of the Graph Pad Prizm software (V5 San Diego, Ca). The most effective leaves with their highest inhibition level were from Ananas comosus (pineapple, 86.50%), Gliricidia sepium (madre de cacao, 84.50%), Chrysophyllum cainito (star apple, 83.75%), Moringa oleifera (horseradish,58.75%), Annona squamosa (sweet sop, 56.00%), Tinospora rumphii Boerl ( \Makabuhay, 55.75%), and Azadirachta indica (Neem, 51.50%), whereby extremely significant (P˂0.001) effect against O. dentatum larvae. Two leaves extracts, from Trichantera gigantea and Leuacaena leucocephala have no significant effect on O. dentatum larvae. The larval migration assay allows initial studies on the anthelmintic effects of the herbs.
菲律宾植物叶片变异对侵染齿状食道虫幼虫的影响
研究了菲律宾9种植物叶片水提物对齿状齿蛾敏感幼虫的驱虫效果。评价的植物种类有:菖蒲、金菊、金菖蒲、菖蒲、辣木、金针菇、印楝、大毛翅、白头菖蒲。从菲律宾塔拉克的卡米林采集的植物叶子在室温下干燥30天,然后研磨。用迁移缓冲液制备了这些植物叶片的水提液。采用体外迁移和抑制实验,评价了其对齿状食道三龄幼虫的驱虫活性。实验置于24孔板中,室温下,每个处理4个重复,分别使用不同浓度的1、2.5、5和10 mg/ml。在20 μm尼龙网过滤器组成的幼虫迁移装置上,每孔约有120只幼虫,在不同处理下孵育20小时,并再孵育2小时,以使活跃的运动幼虫迁移。评价标准以不同处理下被抑制幼虫的平均数量为依据。使用graphpad Prizm软件(V5 San Diego, Ca)的双向方差分析(P=≤0.0001)对百分比抑制进行统计学分析。抑制效果最好的是凤梨叶(凤梨,86.50%)、黄樟叶(可可树,84.50%)、金菊叶(星苹果,83.75%)、辣木叶(辣根,58.75%)、番荔枝叶(甜sop, 56.00%)、褐皮虫叶(\Makabuhay, 55.75%)和印楝叶(印楝,51.50%),其中对齿状蛾幼虫的抑制效果极显著(P小于0.001)。两种叶提取物对齿状夜蛾幼虫均无显著影响。幼虫迁移试验允许对草药的驱虫药作用进行初步研究。
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