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Ancient Biomolecules最新文献

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Ancient DNA Evidence for the Transition from Wild to Domestic Status in Neolithic Goats: A Case Study from the Site of Abu Gosh, Israel 新石器时代山羊从野生状态向驯养状态过渡的古代DNA证据:来自以色列Abu Gosh遗址的案例研究
Ancient Biomolecules Pub Date : 1900-01-01 DOI: 10.1080/13586120290018482
G. K. Bar-Gal, Hamoudi Khalaily, O. Mader, Pierre Ducos, L. Horwitz
{"title":"Ancient DNA Evidence for the Transition from Wild to Domestic Status in Neolithic Goats: A Case Study from the Site of Abu Gosh, Israel","authors":"G. K. Bar-Gal, Hamoudi Khalaily, O. Mader, Pierre Ducos, L. Horwitz","doi":"10.1080/13586120290018482","DOIUrl":"https://doi.org/10.1080/13586120290018482","url":null,"abstract":"In order to determine whether the morphometric criteria used for separating wild and domestic caprines are expressed at the genetic level, Capra bones from the Neolithic site of Abu Gosh, located 12 km west of Jerusalem, Israel were examined. Archaeological excavations at the site revealed the presence of two occupations; the Pre-Pottery Neolithic B (PPNB) dated to circa 9500-8000 bp , and the Pottery Neolithic (PN) dated to circa 7500-5500 bp . The quantity of goat remains in the PPNB assemblage and their morphometric characteristics suggest that they represent wild animals undergoing an early phase of domestication. In contrast, the faunal remains from the PN levels are predominantly those of domestic goats ( Capra hircus ). For the purpose of examining the relationship between morphometry and genetics, mitochondrial DNA (mtDNA) was extracted and cytochrome b and D-loop sequences run on a small sample of caprine bones from both the PPNB and PN levels at the site. Overall, the ancient DNA findings agreed...","PeriodicalId":428796,"journal":{"name":"Ancient Biomolecules","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124773240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
Preservation of nuclear but not chloroplast DNA in archaeological assemblages of charred wheat grains 烧焦小麦籽粒考古组合中细胞核而非叶绿体DNA的保存
Ancient Biomolecules Pub Date : 1900-01-01 DOI: 10.1080/1358612021000010659
M. Banerjee, T. Brown
{"title":"Preservation of nuclear but not chloroplast DNA in archaeological assemblages of charred wheat grains","authors":"M. Banerjee, T. Brown","doi":"10.1080/1358612021000010659","DOIUrl":"https://doi.org/10.1080/1358612021000010659","url":null,"abstract":"We prepared extracts from a sample of charred wheat grains, 2000 years in age, using a standard DNA preparation method in combination with various post-purification procedures designed to remove inhibitors of the polymerase chain reaction (PCR). The resulting extracts gave amplification products when PCRs were directed at the Glu genes, which are located on the wheat nuclear genome. However, repeated attempts to amplify the chloroplast rbcL locus were unsuccessful with these extracts, even though the rbcL PCR system gave positive results with as little as 8.3 pg of modern DNA, compared with a detection limit of 53 pg for the Glu PCRs. We conclude that there is a differential preservation of nuclear and chloroplast DNA in charred wheat grains.","PeriodicalId":428796,"journal":{"name":"Ancient Biomolecules","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116572891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
DNA Diagenesis: Effect of Environment and Time on Human Bone DNA成岩作用:环境和时间对人骨的影响
Ancient Biomolecules Pub Date : 1900-01-01 DOI: 10.1080/13586120290018473
F. Rollo, M. Ubaldi, I. Marota, S. Luciani, L. Ermini
{"title":"DNA Diagenesis: Effect of Environment and Time on Human Bone","authors":"F. Rollo, M. Ubaldi, I. Marota, S. Luciani, L. Ermini","doi":"10.1080/13586120290018473","DOIUrl":"https://doi.org/10.1080/13586120290018473","url":null,"abstract":"We are currently investigating the phenomenon of DNA decay in human bone with the aim of understanding the precise role played by environmental factors such as the action of soil bacteria, fungi, and other microorganisms. With this in view, we sampled a group of 32 human femurs dating back to 1800 ad and submitted them to a series of analyses to check the following parameters: state of preservation of collagen; racemization of aspartic acid; state of preservation of mitochondrial DNA; presence of bacterial DNA; presence of the DNA of lower eukaryotes. The results are consistent with the hypothesis that most of the endogenous human DNA vanishes long before the bone structure undergoes extensive diagenesis and soil microorganisms penetrate in a massive way.","PeriodicalId":428796,"journal":{"name":"Ancient Biomolecules","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130657945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
T1Hydropyrolysis of the Organic Macromolecular Material in the Murchison Meteorite t1 Murchison陨石中有机大分子物质的加氢热解
Ancient Biomolecules Pub Date : 1900-01-01 DOI: 10.1080/1358612021000040421
J. Watson, M. Sephton, G. Love, A. Verchovsky, C. Snape, I. Gilmour
{"title":"T1Hydropyrolysis of the Organic Macromolecular Material in the Murchison Meteorite","authors":"J. Watson, M. Sephton, G. Love, A. Verchovsky, C. Snape, I. Gilmour","doi":"10.1080/1358612021000040421","DOIUrl":"https://doi.org/10.1080/1358612021000040421","url":null,"abstract":"macromolecular material in the Murchison meteorite J.S. WATSON, M.A. SEPHTON, G.D. LOVE, A.B. VERCHOVSKY, C.E. SNAPE AND I. GILMOUR 1 Planetary and Space Sciences Research Institute, Open University, Milton Keynes, Buckinghamshire, MK7 6AA, United Kingdom (j.watson@open.ac.uk) 2 Fossil Fuels and Environmental Geochemistry, NRG, Drummond Building, University of Newcastle, Newcastle upon Tyne, NE1 7RU, United Kingdom 3 School of Chemical, Environmental and Mining Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom","PeriodicalId":428796,"journal":{"name":"Ancient Biomolecules","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130498808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Little Evidence for the Preservation of a Single-Copy Gene in Charred Archaeological Wheat 烧焦的考古小麦中保存单拷贝基因的证据很少
Ancient Biomolecules Pub Date : 1900-01-01 DOI: 10.1080/1358612021000010677
R. Blatter, S. Jacomet, A. Schlumbaum
{"title":"Little Evidence for the Preservation of a Single-Copy Gene in Charred Archaeological Wheat","authors":"R. Blatter, S. Jacomet, A. Schlumbaum","doi":"10.1080/1358612021000010677","DOIUrl":"https://doi.org/10.1080/1358612021000010677","url":null,"abstract":"We investigated 31 samples of charred archaeological cereal grains from the Northern Alpine region, dating from Neolithic to medieval times. One hundred and twenty five DNA extracts from 23 extraction series were screened for the presence of authentic DNA by PCR amplification of a 240 bp fragment from the high molecular weight glutenin subunit gene promoter (HGP) region. Criteria of authenticity adjusted to the features of charred cereal remains were applied. No PCR products were amplified in most extracts. Extraction series with positive results were further analysed with additional primer sets directed at the single-copy HGP region and with primers for the ribosomal (ITS2) or the chloroplast ( rbcL ) DNA. Most positive results of the HGP regions were subsequently considered not authentic, mainly because of molecular inconsistency. In four extracts the successful amplification of the rbcL region, the ITS2 region, or the HGP and the ITS2 region suggested the presence of authentic DNA but further and indep...","PeriodicalId":428796,"journal":{"name":"Ancient Biomolecules","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125477549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Recovery and Analysis of Mitochondrial DNA from Ancient Bones of Common Bream, Abramis brama L. 古鲷骨线粒体DNA的恢复与分析。
Ancient Biomolecules Pub Date : 1900-01-01 DOI: 10.1080/13586120290018527
S. Ciesielski, P. Brzuzan, M. Luczyński
{"title":"Recovery and Analysis of Mitochondrial DNA from Ancient Bones of Common Bream, Abramis brama L.","authors":"S. Ciesielski, P. Brzuzan, M. Luczyński","doi":"10.1080/13586120290018527","DOIUrl":"https://doi.org/10.1080/13586120290018527","url":null,"abstract":"Cyprinid remains were collected from an archaeological site at Wolin, Poland. DNA has been extracted from a 1000 years old bone of common bream, and a 172 bp fragment of the cytochrome b gene of the mitochondrial DNA (mtDNA) successfully amplified by the polymerase chain reaction. The obtained fragment has been sequenced, and showed one point mutation (G ! A) at the position 123 of the gene.","PeriodicalId":428796,"journal":{"name":"Ancient Biomolecules","volume":"288 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133514296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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