古鲷骨线粒体DNA的恢复与分析。

Ancient Biomolecules Pub Date : 1900-01-01 DOI:10.1080/13586120290018527

S. Ciesielski, P. Brzuzan, M. Luczyński

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引用次数: 6

摘要

塞浦路斯人的遗骸是从波兰沃林的一处考古遗址收集来的。从一具1000年前的鲷骨中提取了DNA，并通过聚合酶链反应成功扩增出线粒体DNA (mtDNA)中一个172 bp的细胞色素b基因片段。获得的片段已测序，并显示一个点突变(G !A)基因的123号位置。

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Recovery and Analysis of Mitochondrial DNA from Ancient Bones of Common Bream, Abramis brama L.

Cyprinid remains were collected from an archaeological site at Wolin, Poland. DNA has been extracted from a 1000 years old bone of common bream, and a 172 bp fragment of the cytochrome b gene of the mitochondrial DNA (mtDNA) successfully amplified by the polymerase chain reaction. The obtained fragment has been sequenced, and showed one point mutation (G ! A) at the position 123 of the gene.

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Ancient Biomolecules

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