Recovery and Analysis of Mitochondrial DNA from Ancient Bones of Common Bream, Abramis brama L.

Abstract

Cyprinid remains were collected from an archaeological site at Wolin, Poland. DNA has been extracted from a 1000 years old bone of common bream, and a 172 bp fragment of the cytochrome b gene of the mitochondrial DNA (mtDNA) successfully amplified by the polymerase chain reaction. The obtained fragment has been sequenced, and showed one point mutation (G ! A) at the position 123 of the gene.