Journal of Contemporary Medical Sciences最新文献

{"title":"Effect of Some Metals Ions on Hemolysin Production from Clinical Isolates of Escherichia Coli","authors":"Eman Khadum Juda, K. J. Khalaf","doi":"10.22317/jcms.v10i1.1450","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1450","url":null,"abstract":"Objective:This study aimed to determine effect of some ions metals (Ca+2, Mn+2, Mg+2, Fe+2, K+, Na+, Zn+2) on hemolysin production was performed.\u0000Methods: Eighty Three (83) E. coliisolates collected from different clinical sources from Baghdad Hospitals, performed by using cultural traits, morphological features and biochemical tests, confirmation of identification was done by Vitek 2 system.And it was detected on ability these isolates to production hemolysin by two methods liquid media and agar media.\u0000Results: The results showed that: In agar medium revealed that 6 (7.23 %) isolates of E. coli had the ability for producing this enzyme and 77 (92.77%) did not produce hemolysin. The same results were found in liquid medium. Used the microtitration plate method to study the effect these ions metals on hemolysin production.According to the effect of ions metals, the results also showed that MIC value of ions metals was 500μg/mL, hemolysin production was increased after addition each of(Ca+2,Mn+2, Mg+2,Fe+2,K+,Na+)  the percentage of hemolysis (96.0 %, 93.4 %,  93.2 %, 93.3%,94.2%, 93.7%) respectively while hemolysin production decreased after addition of Zn+2 with the percentage of hemolysis 0.3%.\u0000Conclusion: Based on the influence of metal ions, the findings indicated that hemolysin production exhibited an increase following the addition of each of Ca2+, Mn2+, Mg2+, Fe2+, K+, and Na+ when compared to the control group. Conversely, the introduction of Zn2+ led to a decrease in hemolysin production.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140431405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Genetic Polymorphisms in (STIM1 and ORAI1) on Erythropoietin Resistance in Patients with Chronic Renal Failure on Hemodialysis in Iraq","authors":"Farah f. Alsabbagh, Amal Umran Mosa, Abo Almaali, Hasanain Salah Jafer","doi":"10.22317/jcms.v10i1.1495","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1495","url":null,"abstract":"Objective: This study aims to investigate the correlation between genetic polymorphisms in store-operated calcium channels (SOCs) signaling and resistance to erythropoietin treatment in patients with chronic renal failure (CRF). Anemia is a significant complication of CRF, and understanding the genetic factors contributing to resistance could provide insights into potential mechanisms influencing treatment outcomes. Specifically, the focus is on exploring the relationship between SOCs, a pathway activated by erythropoietin, and resistance to address the challenges associated with anemia in CRF patients. \u0000Methods: In this study, a single nucleotide polymorphism in each STIM1 and ORAI1 gene was selected. Genotyping was done by using allele specific polymerase chain reaction technique and the data was analyzed through the Statistical Package for the Social Sciences. 112 patients with CRF on hemodialysis were enrolled 58.9% of them were men and the mean age was 50.94±13.42. \u0000Results: We found that CC genotype of ORAI1 gene has a higher hemoglobin level, AA genotype of STIM1 has a higher EPO level, the interaction of the two genes showed that CCGG group has a higher Hb level and is considered good responder but represents only 4.5% of the patients \u0000Conclusion: After all the genetic polymorphisms of SOCs genes ORAI1 and STIM1 have no significant impact on erythropoietin resistance in patients with CRF on hemodialysis in Iraq.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140431177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Collagen/PLA-based Nanofibrous Scaffold to Support PC12 Cells and Neural Repair","authors":"Muhammad Aseer, Mohammad Taleb, Zohreh Arabpour, Qaisar Abbas Bhatti, Hossein Ghanbari","doi":"10.22317/jcms.v10i1.1493","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1493","url":null,"abstract":"Objective: This study attempts to modify Polylactic Acid (PLA) with the natural polymer Collagen (Coll), to develop materials such as an electrospun scaffold that have better mechanical stability and biocompatibility. Retinoic acid (RA), a bioactive material that promotes nerve growth, is to be added to the nanofiber scaffolds as part of this project. \u0000Methods: One of the most important methods we employed in this work to create nanofibrous scaffolds was electrospinning. \u0000Results:  The synthesized nanofiber scaffold exhibited a diameter of 255±40 nm and a tensile strength of 175±10.4 N, providing sufficient support for native peripheral nerve repair. The inclusion of Coll enhanced the scaffold's hydrophilic behavior (contact angle: 56±4°), ensuring stability in aqueous solutions. In addition, cell adhesion and proliferation are demonstrated to be improved by PLA composite nanofibers based on Collagen, while PC12 cell adhesion and proliferation are further improved by RA. \u0000Conclusion: Based on their biodegradability, robust mechanical properties, and porous structure, these scaffolds are excellent choices for nerve tissue engineering, according to our findings. The significant increase in PC12 cells' adhesion and proliferation upon the addition of RA demonstrates the cells' potential for nerve repair.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluate The Effect of Coenzyme Q10 on The Prevention of Doxorubicin-Induced Acute Cardiotoxicity in Rats.","authors":"Ola Sadeq AL-Shimaysawee, Fadhil A. Rizij, Rafid Mohammed Ali, Hassan Wasfi","doi":"10.22317/jcms.v10i1.1483","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1483","url":null,"abstract":"Objective: This study will evaluate the potential protective effects of coenzyme Q10 on DOX-induced cardiotoxicity in female rats, manifested by changes in biochemical parameters in tissue and serum samples histopathological differences, and compare their changes.\u0000Methods:24 female rats were divided into three groups based on weight. The first group received saline, the second group received a cumulative dose of 15 mg/kg of DOX via IP injection, and the third group was pre-treated with CoQ10 before receiving DOX. The data were analysed using a one-way ANOVA test with a Bonferroni post hoc test to compare the markers and histopathological changes in different groups. The GraphPad Prism version 8.1 was used to do the statistical analysis.\u0000Results: As indicated by a statistically significant increase (P<0.0001) in TNFα and ICAM-1 level, doxorubicin-induced cardiotoxicity. Additionally, the level of GSH, SOD, and caspase-3 did not differ significantly between the DOX+CoQ10 group and the control group. Furthermore, lesions and histological alterations were induced by the substance. Significant reductions in cardiotoxicity were observed with the administration of coenzyme Q10, as indicated by notable increases (P<0.0001) in SOD, GSH, and TNFα and significant decreases (P<0.0001) in caspase 3 when compared to the DOX group. Also, the CMYO score and lesions exhibited a substantial improvement.\u0000Conclusion: In this investigation, coenzyme Q10 exhibited cardioprotective effects against DOX-induced damage to the mouse heart. This phenomenon potentially pertains to inhibiting and safeguarding against oxidative stress, the pathway of apoptosis, and the inflammatory response.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review: Hospital Acquired Infection in Some Gram Negative Pathogenic Bacterial Strains","authors":"H.S. Alkhowaiter, Abdullah AL-Jaddawi, Mohamed Abu-Zeid, Salah E. M. Abo-Aba","doi":"10.22317/jcms.v10i1.1475","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1475","url":null,"abstract":"Objective: This review aims to update healthcare workers on the current scientific understanding of hospital-acquired infections, witha focus on describing the pathophysiology and patterns of antimicrobial resistance, particularly concerning Pseudomonas aeruginosa,Acinetobacter baumannii, and Klebsiella pneumoniae.\u0000Methods: Data on hospital-acquired infections were collected globally, with a specific emphasis on the Eastern Mediterranean, South-East Asia, Europe, and the Western Pacific regions. Infection rates, predominant pathogens, and antimicrobial resistance patterns were analyzed to provide insights into the current landscape of nosocomial infections.\u0000Results: Hospitals in the Eastern Mediterranean and South-East Asia regions reported the highest rates of nosocomial infections (11.8%and 10.0%, respectively), while rates in Europe and the Western Pacific were 7.7% and 9.0%, respectively. Infections typically arise frominvasive medical equipment and surgical operations, with lower respiratory tract and bloodstream infections being particularly hazardous. Gram-negative bacterial infections, notably, exhibit worrisome antibiotic resistance patterns, potentially developing multiple mechanisms against various antibiotics.\u0000Conclusion: The emergence of antimicrobial resistance presents a significant threat to patient safety, compounded by challenges indiscovering new antibiotics. Factors such as high costs and lengthy drug development processes contribute to this concern. Healthcareworkers must remain abreast of evolving antimicrobial resistance patterns, especially concerning pathogens like Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae, to implement effective infection control measures and preserve the efficacy of existing antimicrobial agents.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Analysis of Medical Students’ Learning Experiences in Small Group-Based Teaching in Kurdistan Region-Iraq","authors":"Mahmoud Ali Abdi, Dilveen Mikhail, Basheer Abdi, Dastan Mustafa, Sawen Ibrahim","doi":"10.22317/jcms.v10i1.1418","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1418","url":null,"abstract":"Objective: This study aims to evaluate the learning styles, activities, and experiences of medical students within the context of small groupbased teaching in the Kurdistan Region of Iraq.\u0000Methods: A cross-sectional study was conducted between February and May 2022 at Zakho College of Medicine, involving students from various medical colleges in the region. A pre-designed questionnaire was administered both in-person and online. Comprising two main parts in addition to demographic information, the questionnaire assessed students' learning styles based on O'Brien's modality learning channel preference and their preferences and experiences in small-group settings.\u0000Results: The sample included students from the 1st to the 5th stage, totaling around 400 participants. The cohort was predominantly female (60.05%) and largely from the 2nd stage of medical education (55.03%). Visual Learning was the most common learning style, particularly among female students. Significant variations in learning styles were noted across different academic stages and universities. Small-group activities and formative assessments were generally favored. While most students were either \"satisfied\" or found it \"natural\" concerning group size, dissatisfaction was prominent regarding available teaching resources. Statistical analyses confirmed significant differences in learning styles, preferred teaching methods, and feelings about the learning environment.\u0000Conclusion: The study offers critical insights into the diverse learning preferences and experiences of medical students in small-group settings. Despite a general preference for Visual Learning and small-group activities, significant variations exist, warranting a nuanced approach to educational strategy. Given the diverse learning preferences and significant dissatisfaction with available resources, a multifaceted educational approach is recommended, including diverse teaching and assessment methods and increased investment in teaching resources.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140431186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Validity of Computed Tomography Guided Lung Tru- cut Biopsies in Achieving Accurate Tissue Results","authors":"B. M. Khashman, K. Mohson, Omar Abdul Jabbar Abdul Qader, Kifah H. Abdul Ghafour","doi":"10.22317/jcms.v10i1.1499","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1499","url":null,"abstract":"Objective: This study aims to assess the efficacy of CT-guided true-cut biopsy as a less invasive and cost-effective diagnostic technique for peripherally placed lung lesions. \u0000  \u0000Methods: fourty patients with solitary lung nodule were involved in this study, true cut biopsies under Ct guide was taken then processed for routine H&E staining. \u0000  \u0000Results: different pathological features can be identified with different pathological features giving primary diagnostic screening for lung cancer \u0000  \u0000Conclusion: CT guided thoracic lesion biopsy is very efficient, cost-effective and less invasive technique when compared with the thoracic surgery","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140429212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship between Vitamin D, Body Mass Index and some Dietary Products with Acne Vulgaris; A Case-Control study in Baghdad/Iraq","authors":"Sarah Haider Zalzala, Besmah Mohammad Ali, Zahraa Saeed Fahad","doi":"10.22317/jcms.v10i1.1459","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1459","url":null,"abstract":"Objective: To investigate the association of vitamin D level and moderate to severe acne as a primary outcome, and its association with BMI, dairy products, and chocolate consumption.\u0000Subjects and Methods: A case-control study was conducted in a dermatology outpatient clinic. Patients, diagnosed with moderate to severe acne vulgaris that warrant treatment with oral antibiotics, and had global acne grading system (GAGS) score of >=19 were eligible to be selected as cases. Aged matched, with no or mild cases of acne were eligible to be selected as controls. The participant height, weight, BMI, and serum circulating 25-hydroxyvitamin D level were measured. A modified food frequency questionnaire was used for dietary history.\u0000Results: Comparing Cases (N = 101) and Control (N = 134), the mean of vitamin D level in cases were lower than that in the controls, however no statistically significant difference is observed. There were statistically insignificant differences between the studied groups regarding milk (whole, low fat, skimmed and any type of milk), cheese, yogurt, other dairy products, and chocolate consumption. However, a statistically significant disparity in weight is noted, but not in BMI.\u0000Conclusion: The results did not reveal any statistically significant associations between vitamin D level, BMI, dairy and chocolate consumption, and moderate to severe acne. However, it is important to note that this does not definitively rule out the possibility of any potential relationships or effects.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Pregnant Knowledge and Adherence Regarding Supplemental Iron, Folic acid, Calcium, and Vitamins During Pregnancy","authors":"Walaa A. Karim, I. S. Rabea, H. H. Abdulwahid","doi":"10.22317/jcms.v10i1.1492","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1492","url":null,"abstract":"Objectives:  The aim of the present study is to evaluate the knowledge as well as the  rate of adherence to supplement medications. \u0000Methods: The cross-sectional study was carried out at a teaching hospital for obstetrics and gynecology in Karbala with 100 pregnant women at the second trimester, data were collected using two questionnaires, one about knowledge and other about adherence to micronutrient supplementation, laboratory tests were used to determine the levels of Hemoglobin and plasma level of calcium, and vitamin D. \u0000Results: the overall adherence rate to supplement medication among pregnant women was low in spite of good knowledge score. Biochemical laboratory results showed more than half of participants were found to have haemoglobin     \u0000Less than normal level. Most of participant confirmed to have deficiency of plasma 25-Hydroxy-vitaminD, and more than two third of them have normal calcium level. \u0000Conclusion: the present study revealed that most pregnant women have a poor adherence to micronutrient supplementation, therefore there is a need for interventional strategies to optimize adherence.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140428194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moexipril Improves Renal Ischemia/Reperfusion Injury in Adult Male Rats","authors":"Esraa H. Alsaaty, Ali M. Janabi","doi":"10.22317/jcms.v10i1.1477","DOIUrl":"https://doi.org/10.22317/jcms.v10i1.1477","url":null,"abstract":"Objective: The goal of this study is to see if moexipril can protect rats against renal ischemia/reperfusion injury.\u0000Methods: Overall twenty-eight males of rats were divided randomly into four groups (7 rat each group). Sham group: Except for ischemiainduction, these rats underwent IP anesthesia and surgery. Induced group: This group rats were anesthetized and given a midlinelaparotomy to induce bilateral renal ischemia for 30 min and 2 hours of reperfusion. DMSO group: Rats received DMSO IP injection 30 minbefore ischemia and subjected to 30 min bilateral ischemia and reperfusion for 2 hours, DMSO is a vehicle of moexipril and considered ascontrol. Moexipril group (pretreated group): moexipril was given in a dose 0.3 mg/kg I.P. injection 30 min before ischemia.\u0000Results: Renal IRI as indicated by a significant increase (P < 0.05) in urea, creatinine, NF-KB P65, IL-1β, and caspase-3 level, while GSH,SOD, and Bcl-2 levels significantly (P < 0.05) reduced in Renal tissues of rats in the induced group compared to sham group. Moexiprilpretreatment significantly (P < 0.05) ameliorate RIRI as suggested from significant lowering in urea, creatinine, and inflammatory markers(NF-KB P65 and IL-1β). The renal level of oxidative marker (SOD and GSH) and anti-apoptotic marker Bcl-2 were significant decreased(P < 0.05) and also significantly increase (P < 0.05) in caspase-3 level with moexipril group in comparison to induced group.\u0000Conclusion: By inhibiting oxidative stress, inflammation, and the apoptotic pathway, moexipril significantly protect from renal ischemiareperfusion in rats.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}