Journal of Contemporary Medical Sciences最新文献

{"title":"MOR/ERK signalling pathway promotes the malignant progression of residual hepatocellular carcinoma cells after insufficient radiofrequency ablation","authors":"Qianxi Yin, Fengjuan Li, Feiyan Gao, Xiaoying He, Ying Liu, Yang Liu, Jiwei Liu, Shujun Liu, Guoqun Jia, Libing Ma","doi":"10.22317/jcms.v11i2.1691","DOIUrl":"https://doi.org/10.22317/jcms.v11i2.1691","url":null,"abstract":"Objective: Radiofrequency ablation (RFA) is the first-line of treatment for unresectable early hepatocellular carcinoma (HCC). However, the accelerated malignancy progression of residual HCC cells post-RFA pose a major obstacle to its clinical application. The mechanisms underlying the increased malignancy of residual HCC cells following RFA require further investigation. Methods: In the present study, HepG2 cells, an established human HCC cell line, were subjected to repeated heat treatment to simulate residual HCC cells after RFA, surviving cells were designated as HepG2-H cells. Malignancy parameters and μ-opioid receptor (MOR) expression levels were compared between HepG2 and HepG2-H cells. RNA sequencing was subsequently performed to identify differentially expressed genes (DEGs) between the two groups of cells, and pathways enrichment analysis was conducted to investigate signaling pathways potentially driving HepG2-H cells malignancy. Results: HepG2-H cells exhibited significantly elevated malignancy compared to HepG2 cells, as evidenced by enhanced proliferation, migration, and colony formation. MOR expression was significantly upregulated in HepG2-H cells. RNA sequencing revealed that DEGs between the two groups were predominantly enriched in cancer pathways. Furthermore, phosphorylated ERK1/2 (P-ERK1/2) levels were 1.18-fold higher in HepG2-H cells, and the inhibition of MOR activity could reduce both the level of P-ERK1/2 and the malignancy of HepG2-H cells. Conclusion: Our findings suggest that the activation of MOR/ERK signaling pathway contributes to the malignant progression of residual HCC cells post-RFA. Targeting MOR may offer a novel therapeutic strategy to mitigate tumor recurrence following RFA.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"11 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exopolysaccharides from Rhizopus nigricans inhibit proliferation and induce apoptosis in human lung adenocarcinoma A549 cells","authors":"Mengmeng Li, Jinhui Wu, Xiuwen Ni, Chunyan Liu, Jun Xu, Hongyun Wang, Zhang Li","doi":"10.22317/jcms.v11i2.1726","DOIUrl":"https://doi.org/10.22317/jcms.v11i2.1726","url":null,"abstract":"Objective: To analyse the effects of an exopolysaccharide (EPS2-1) obtained from the fermentation broth of Rhizopus nigricans on the viability and apoptosis of human lung adenocarcinoma (A549) cells. Methods: Lung cancer cells were cultured in vitro and divided into the control and exopolysaccharide-treated groups. CCK-8 and colony formation assays were performed to measure the proliferation of A549 cells. Caspase activity was measured using a colorimetric assay. The apoptosis rate, reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) were evaluated using flow cytometry. Results: EPS2-1 effectively suppressed A549 cell proliferation (p < 0.05). At the same time, EPS2-1 significantly increased the enzyme activity of caspase-8 and caspase-3 compared with that in the control group (p < 0.05) and facilitated the apoptosis of A549 cells. Furthermore, EPS2-1 increased the accumulation of intracellular ROS and induced a reduction in MMP in A549 cells. These findings suggest that EPS2-1 initiated apoptosis in A549 cells via the mitochondrial pathway. Conclusion: EPS2-1 significantly inhibited A549 cell proliferation and induced apoptosis via ROS accumulation and mitochondrial dysfunction, suggesting its potential as an anti-cancer agent. This study provides the first evidence that Rhizopus nigricans-derived polysaccharides exhibit induce-apoptosis activity on A549 cells through ROS-mediated mitochondrial dysfunction, establishing a novel pharmacological foundation for fungal polysaccharide research and development of natural antitumor agents.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"11 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of New Thiourea Derivatives as Potential SIRT Inhibitors","authors":"Mohammed A. Abbas, Ayad A. Al-Hamashi","doi":"10.22317/jcms.v10i5.1627","DOIUrl":"https://doi.org/10.22317/jcms.v10i5.1627","url":null,"abstract":"Objective: This study aimed to design and synthesize novel thiourea-based compounds targeting Sirtuins (SIRTs), a class of histone deacetylase enzymes implicated in various cellular processes, including cancer, and to evaluate their cytotoxic potential against colon cancer cells. Methods: Virtual modeling and molecular docking studies were conducted to design the compounds and assess their interaction with SIRT enzymes. Compounds showing acceptable docking scores were selected for organic synthesis. The final compounds (MA-1 to MA-6) were synthesized and characterized using FTIR, ¹H-NMR, and ¹³C-NMR spectroscopy. Cytotoxicity studies were performed on SW480 colon cancer cells to evaluate the biological activity of the synthesized compounds. Results: All synthesized compounds exhibited promising cytotoxicity against SW480 colon cancer cells, with MA-2 demonstrating a particularly strong effect. The half-maximal inhibition concentration (IC50) of the compounds was in the single-digit micromolar range, comparable to established SIRT inhibitors Selisistat and AGK2. Conclusion: The thiourea-based compounds synthesized in this study, especially MA-2, showed significant potential as SIRT inhibitors with cytotoxic activity against colon cancer cells. These results warrant further investigation into their therapeutic potential.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"10 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147921390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of CYP2D6 polymorphisms on the efficacy of tamoxifen in Iraqi women with breast cancer","authors":"Sura Sagban, Ahmed S. Sahib, Ahmed S. Abdulamir, Haitham M. Kadhim","doi":"10.22317/jcms.v9i6.1396","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1396","url":null,"abstract":"Background: Tamoxifen remains the primary therapeutic option for breast cancer treatment and for the primary prevention of metastatic disease. Understanding the variability in tamoxifen response may provide clues to help personalize cancer treatment as well as maximize the therapeutic efficacy. One area of particular interest that merits attention in hormonal therapy is the impact of the CYP2D6 genetic polymorphisms on tamoxifen metabolism and consequently, on patient The aim of the present study was to investigate the impact of the CYP2D6 genetic polymorphism on clinical outcome in Iraqi breast cancer patients who were candidates for tamoxifen therapy. Methods: Comprehensive CYP2D6 genotyping was performed in 140 Iraqi women with breast cancer who were women on adjuvant treatment with tamoxifen. Breast cancer patients recruited into the study were divided into two groups: seventy breast cancer women who had no history of recurrence at the time of sampling and had a long time on tamoxifen without recurrence and seventy breast cancer women who had recurrence at the time of sampling after one year of treatment with tamoxifen therapy. Recurrence free survival (RFS) was determined in the recruited patients.  Results: Multiple genetic variants of the gene encoding the CYP2D6 enzyme were detected with significant differences in their frequencies and percentages in both recurrent and non-recurrent groups of breast cancer patients. The findings of this study suggest that interindividual variation in clinical outcome may be related to genetic variation in CYP2D6 enzyme, which is characterized by variable RFS periods. Conclusions: This study revealed that the CYP2D6 enzyme of breast cancer patients who participated in this study is highly polymorphic. The CYP2D6 gene of study participants exhibited different allelic combinations with variable frequencies. The multiple genetic variants (alleles) of the gene encoding the CYP2D6 enzyme exhibited significant differences in their frequencies and percentages in both recurrent and non-recurrent groups of breast cancer patients. .The study revealed variable Recurrence free survival (RFS) with highly polymorphic gene. Our study quotes the presence of increased CYP2D6 enzyme polymorphism is associated with variable clinical response.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"3 6","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of COVID-19 on the quality of life among University students with obsessive-compulsive disorder","authors":"Reem Almustafa, Abdulaziz Alkhoshi, Jumana Alshaikh, Reema Ayoub, Zainalabden Aljifri, Sulhi A. Alfakeh","doi":"10.22317/jcms.v9i6.1474","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1474","url":null,"abstract":"Background: The coronavirus disease 2019 (COVID-19) pandemic has necessitated precautionary measures to prevent the transmission of the virus, affecting individuals with obsessive-compulsive disorder (OCD). We investigated the impact of the COVID-19 pandemic on the quality of life of university students with OCD. Methods: Students with validated confirmed diagnosis of OCD at King Abdulaziz University, Jeddah, received two online surveys: the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) to approve the diagnosis and the 36-Item Short-Form Health Survey (SF-36) to assess symptom severity and overall quality of life during the pandemic. Students with psychotic disorders were excluded from this study. Results: The majority were females (62.5%), with an average age of 23.13 years. The participants had mild to moderate of OCD symptoms. The SF-36 role limitation due to emotional problems domain was significantly associated with the level of OCD symptoms (p=0.023), with higher scores reported for those with mild symptoms. Age was positively correlated with social functioning and pain domains but negatively correlated with Y-BOCS scores. The general health domain was significantly associated with current OCD symptoms and family history of OCD diagnosis. Conclusions: The self-reported decline in the severity of OCD symptoms in adult students indicates that the participants in our study with diagnosable OCD perceive an improvement in their symptoms from the previous year.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"13 22","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Talh Honey, Whey Protein, and Collagen Based Novel Formulation Against Wound-Associated Skin Microbiota","authors":"Hanaa Abbas Yamani, Muna Mordi Al-Zahrani","doi":"10.22317/jcms.v9i6.1454","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1454","url":null,"abstract":"Diabetes, immunosuppressive treatments, and obesity could severely impair the complicated wound-healing process. The skin serves as a dynamic and complex ecosystem for fungi, archaea, viruses, and bacteria, which collectively represent the skin microbiota. These microbes infect the underlying tissues through the injured skin. Delayed wound healing could make the injured site more prone to microbial infection. Several cultures have utilized honey as a traditional medicine for centuries. It was primarily used to treat wounds (infected and chronic) even before the discovery of bacteria. Honey is known for its antimicrobial properties. Whey protein is rich in growth factors, which promote cell proliferation and differentiation. They also facilitate tissue repair and can effectively stimulate the wound-healing process. Therefore, this study evaluates a novel wound-healing formulation consisting of honey, whey protein, and collagen against the skin microbiota associated with delayed wound healing. A rat excisional model was followed to test the formulation through pathogen contraction rate on different days after the onset of wounds. The tested formulation effectively promoted the wound-healing process in rats.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"15 11","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The DNA methylation of the interleukin6 as a biomarker for the early detection of colorectal cancer","authors":"Sakar Ahmed Abdullah, Dlnya Assad Mohamad","doi":"10.22317/jcms.v9i6.1440","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1440","url":null,"abstract":"Objectives: To identify the DNA methylation pattern of the interleukin6 promoter region in colorectal cancer patients and colorectal polys patients that could be a biomarker for early detection of colorectal cancer. Methods: For this purpose, we examined the DNA methylation pattern of the promoter region of the IL6 specifically 358 bp (-292 to +67) including 7 CpG sites (-228, -213, -163, -119, +10, +19, +25) in a total of ninety samples with thirty samples each for controls, cancer patients, and colorectal polyps using the bisulfite conversion sequencing method. Result: Our results indicate that CpG +25 and CpG -119 can serve as biomarkers for early detection of colon cancer, showing a significant difference in P-values of 0.001 at CpG +25 and 0.004 at CpG-119. The hypomethylation of CpG -119 in cancer groups facilitates the binding of the methyl-sensitive transcription factor Sp1. It enhances the overexpression of IL6 besides hypermethylation of CpG +25 that prevents binding of the methyl-sensitive insulator CTF, unbinding the insulator to the promoter region of the cancer samples makes the promoter region open access to the TFs that enhances overexpression. Furthermore, a remarkable non-recorded SNP at CpG -228 was observed in 98.6% of the enrolled groups. Conclusion: In the state of DNA methylation, IL 6 could contribute to the onset of colorectal polyps and colorectal polyps cancer due to a significant level of methylation in CpG +25 and CpG -119.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"21 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid cancer, risk factors, clinical features, prognosis, and its incidence preference in Saudi populations: review","authors":"A. Alhozali","doi":"10.22317/jcms.v9i6.1476","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1476","url":null,"abstract":"Within the diverse cancers reported, the epidemiology of thyroid cancer increased 3-fold in the latest years which may be due lifestyle changes, lack of cancer awareness, lack of screening, and early cancer investigations and detection programs in addition to genetics and deficiency of iodine and vitamin D.  The aim of this review was to discuss Thyroid cancer incidence, risk factors, and clinical features and prognosis. The cause of thyroid cancer is unknown, but because the thyroid gland is very sensitive to radiation, exposure to radiation can cause precancerous changes. For unclear reasons, thyroid cancer like almost all diseases of the thyroid, occurs about 3 times more often in women than in men at any age, but the risk peaks earlier for women at 40 to 50 years. Several inherited conditions have been linked to different types of thyroid cancer but some patients with thyroid cancer do not have inherited conditions or a family history of the disease. Four general types of thyroid cancer were explained, Papillary, Follicular, Medullary, and Anaplastic. Over the last decade, the incidence of Papillary and Follicular thyroid cancers increased at the same time.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"24 17","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apelin as a potential marker in Iraqi children with Type 1 Diabetes Mellitus","authors":"Noor Thair Tahir, Israa Qusay Falih, Mithal R. Alkubaisi, Abdilya Riyadh Alabdaly","doi":"10.22317/jcms.v9i6.1425","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1425","url":null,"abstract":"Apelin is a peptide has receptors in several locations, involved in regulating cardiac homeostasis, angiogenesis and metabolic regulation. It is also consider as adipokine secretion by adipocytes. This study aimed to evaluate apelin levels and other biochemical parameters in Iraqi pre-pubertal and pubertal T1DM. Ninety children participated in current study, divided into three groups: 30 pre-pubertal patients with type 1 diabetes, 30 at pubertal patients with type 1 diabetes, and 30 healthy subjects. Measurements of fasting blood glucose (FBS), random blood sugar (RBS), glycosylated hemoglobin (HbA1c), apelin, lipid profile, thyroid hormones, Blood urea, and serum creatinine were perform for the comparison groups.Apelin has a positive correlation coefficient (P< 0.01) with FSB, TG and (P <0.05) with BMI, HbA1c, TC, TSH also has a negative correlation coefficient with HDL-C, in pubertal and pre-pubertal respectively.  The relationship among levels of apelin, glucose, and insulin sensitivity shows the participation of apelin in the pathogenesis of diabetes and its rise in adulthood is an indicator to beginning diabetes complications, Aside from the potential use of apelin as a diagnostic marker in early discrimination of cardiovascular disease.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"17 4","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Nurse’s job Satisfaction upon Quality of Nursing Care for Patients Undergoing Hemodialysis","authors":"Luay A. Mansoor, Tahseen Rajab Muhammad","doi":"10.22317/jcms.v9i6.1458","DOIUrl":"https://doi.org/10.22317/jcms.v9i6.1458","url":null,"abstract":"Introduction: Job satisfaction among nurses is a global concern for its crucial impact on nursing shortage and quality of care Objectives: This study aimed to asses’ nurses' job satisfaction levels at hemodialysis units and to find out the relationship between nurses' job satisfaction and quality of nursing care. Methods: A descriptive cross-sectional design study was carried out among nursing working in hemodialysis units at Baquba Teaching Hospital and Baladrooz general Hospital, study starting from 14th December 2022 to 10th March 2023.A non-probability (purposive) sample of (80) nurses working in hemodialysis units in Baquba and Baladrooz Hospital . The study instruments consist of three parts; Part one is involved the demographic information of nurses (8) items; the second part is involved Job Satisfaction Scale for Clinical Nurses (JSS-CN), it is comprised of (38) items and observational checklist for nurses practices of hemodialysis procedures consist from (29) items  .Content validity of the questionnaire was determine through a panel of (12) experts .Reliability of the instrument scales is determined by using Pearson Correlations )0.87,084) for of job satisfaction scale and observational checklist for nurses practices respectively . The collected data will be categorized, scored, tabulated, and analyzed by computer using statistical package for social science (SPSS) version 25. Descriptive statistics will be used in the form of frequency distribution and percentages. Pearson correlation coefficient statistical tests were applied for data analysis. Results: Findings of the current study showed that (75%,65%), of the studied nurses offered moderate job satisfaction level and good quality of nursing care respectively. There was highly statistically significant relation between job satisfaction and quality of nursing care offered to patient undergoing haemodialysis. Conclusion: This study concluded that, nearly three quarter of the nurses were coping with their jobs and they are satisfied to certain extent. However, nearly two third of them offered good quality of nursing care to patient undergoing hemodialysis","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"14 15","pages":""},"PeriodicalIF":0.2,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}