Microbiologia Medica最新文献_第8页

Colistin-resistant microorganisms and cystic fibrosis: microbiological surveillance in an Italian Children’s Hospital 粘菌素耐药微生物和囊性纤维化:意大利儿童医院的微生物监测

Microbiologia Medica Pub Date : 2019-05-22 DOI: 10.4081/MM.2019.8163

A. Montemari, V. T. G. Assanti, G. Linardos, G. Bonaventura, G. Ricciotti, E. Fiscarelli

{"title":"Colistin-resistant microorganisms and cystic fibrosis: microbiological surveillance in an Italian Children’s Hospital","authors":"A. Montemari, V. T. G. Assanti, G. Linardos, G. Bonaventura, G. Ricciotti, E. Fiscarelli","doi":"10.4081/MM.2019.8163","DOIUrl":"https://doi.org/10.4081/MM.2019.8163","url":null,"abstract":"Several advances in the medical field are often dependent on the ability to fight infections with the use of antibiotics, including joint replacements, organ transplants, and cancer therapy. The capacity of the bacteria to adapt to and escape from the mechanisms of action of antibiotics makes the antimicrobial resistance a serious public health problem worldwide. Polymyxin E colistin has rarely been used because of its nephrotoxicity and neurotoxicity. More recently, the emergence of multi-drug resistant bacteria as carbapenem-resistant Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa and the re-evaluation of its pharmacokinetic properties have led to a resurgence of colistin as a treatment option, contributing to select resistant strains. Investigating the phenomenon of colistin-resistance in gram-negative bacteria, especially P. aeruginosa, is now mandatory, particularly after identification of a plasmid-mediated mechanism for the resistance to colistin (mcr) in Enterobacteriaceae strains, a mechanism transferable to other species. In this study, we investigated colistin-resistance in gram-negative bacteria isolated from respiratory secretions of cystic fibrosis patients in follow-up at Children’s Hospital Bambino Gesù of Rome.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122114577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recurrence of measles in central Italy: the experience of a hospital in Rome 意大利中部麻疹复发:罗马一家医院的经验

Microbiologia Medica Pub Date : 2019-05-22 DOI: 10.4081/MM.2019.7999

A. Petrucca, A. Alari, S. Papadopoulou, C. Petrucci, I. Santino

{"title":"Recurrence of measles in central Italy: the experience of a hospital in Rome","authors":"A. Petrucca, A. Alari, S. Papadopoulou, C. Petrucci, I. Santino","doi":"10.4081/MM.2019.7999","DOIUrl":"https://doi.org/10.4081/MM.2019.7999","url":null,"abstract":"Measles continue to be a major public health issue worldwide with high morbidity and mortality rates. The disease is still endemic in Europe and during 2017 a vast outbreak was described in Italy, Romania and Hungary, which led to thousands of new cases and several deaths. In Italy, 3931 confirmed cases of measles were reported to the Italian national surveillance system from many Italian administrative regions; Lazio, in central Italy, exhibited the highest number of infected patients 1322 (33.63%) and as well as the highest incidence. In this study, we describe the results of a retrospective analysis, carried out during 2016 and 2017, concerning the measles antibody prevalence in patients and healthcare workers attending the Sant’Andrea Hospital of Rome (Lazio). A total of 94 patients (median 30 years of age) were screened in 2016, and 316 (median 40 years of age) during 2017, with an increase of 236% compared to previous year. During 2017, 41 confirmed cases of measles were reported while none in 2016 (P<0.007), and we found a suboptimal immunization coverage in our cohort of patients. Furthermore, measles surveillance of Sant’Andrea healthcare workers during the study period involved 208 personnel units (median >47 years of age) and only one confirmed measles infection was recorded in 2017. These results suggest that there is still an unvaccinated portion of the adult population, who sustain the endemic circulation of measles in Italy. In addition to reach herd immunization on children of 2 years old, catch-up vaccination campaign targeting adult population in Italy and other European countries needs to be implemented to prevent future measles outbreak.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123574190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency and antibiotic susceptibility pattern of uropathogenic agents of urinary tract infections among asymptomatic diabetic patients in Okada community, southern Nigeria 尼日利亚南部Okada社区无症状糖尿病患者尿路感染尿路病原体的频率和抗生素敏感性

Microbiologia Medica Pub Date : 2019-05-22 DOI: 10.4081/MM.2019.8077

Ruqayyah B. Adegbite, H. Ojokuku, Kamoru A. Adedokun, M. Oyenike, Ramat T. Kamorudeen

{"title":"Frequency and antibiotic susceptibility pattern of uropathogenic agents of urinary tract infections among asymptomatic diabetic patients in Okada community, southern Nigeria","authors":"Ruqayyah B. Adegbite, H. Ojokuku, Kamoru A. Adedokun, M. Oyenike, Ramat T. Kamorudeen","doi":"10.4081/MM.2019.8077","DOIUrl":"https://doi.org/10.4081/MM.2019.8077","url":null,"abstract":"Massive evidence showed that patients with diabetes have a high risk of urinary tract infections. We studied the frequency of potential urinary uropathogens among diabetic patients and identified their antimicrobial susceptibility patterns. This was a prospective hospital-based study conducted at the Department of Medical Laboratory Science, Igbinedion University Teaching Hospital, Southern Nigeria, between January 2014 and May, 2014. We included 240 previously confirmed diabetic patients (women, n=70 and men, n=170) who were regularly followed up without prior treatment with any antimicrobial therapy and within the age range of 26-75 years. Patient personal history data and midstream urine samples were collected. Urine samples were processed in the laboratory following a Standard Laboratory Protocol. Escherichia coli, Staphylococcus aureus, Klebsiella spp. and Candida spp. were isolated in this study. A significant bacteriuria count was estimated in 12.5% of the sampled population, while 17.1 and 10.6% were estimated in females and males, respectively. Similarly, candiduria was found in female, male and total sample in 12.9%, 2.9%, and 5.8%, in that order. According to antimicrobial sensitivity testing, the Gram-negative bacilli isolated were highly sensitive to nitrofurantoin followed by ofloxacin, gentamycin and least sensitive to cefuroxime. Estimation of potential uropathogens among asymptomatic diabetic populations may avert possible urinary tract infections and their possible complications ultimately and thus prevent possible advanced renal diseases.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133525021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular epidemiology of non-viral sexually transmitted infections in the central Alpine province of Bolzano, northern Italy from April 2016 to March 2017 2016年4月至2017年3月意大利北部阿尔卑斯中部博尔扎诺省非病毒性性传播感染的分子流行病学研究

Microbiologia Medica Pub Date : 2018-12-19 DOI: 10.4081/MM.2018.7852

R. Aschbacher, F. Romagnoli, E. Masi, V. Pasquetto, F. Perino, K. Eisendle, M. Braghetto, S. Messini, S. Delbue, E. Pagani

{"title":"Molecular epidemiology of non-viral sexually transmitted infections in the central Alpine province of Bolzano, northern Italy from April 2016 to March 2017","authors":"R. Aschbacher, F. Romagnoli, E. Masi, V. Pasquetto, F. Perino, K. Eisendle, M. Braghetto, S. Messini, S. Delbue, E. Pagani","doi":"10.4081/MM.2018.7852","DOIUrl":"https://doi.org/10.4081/MM.2018.7852","url":null,"abstract":"Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium are established or presumed as (??) STI pathogens. The present study aims at ng describing the one-year molecular epidemiology of these seven pathogens in the Province of Bolzano, Northern Italy. From April 2016 to March 2017, a total of 2,949 patients, mainly females, were enrolled and 3,427 urine, vaginal, endocervical and/or urethral samples were subjected to simultaneous analysis of the seven pathogens by means of Real Time Polymerase Chain Reaction (AnyplexTM II STI-7 Detection Kit Seegene, Seoul, Korea). At least one of the seven microorganisms was detected in 40.7% of patients, with an uneven distribution: 43.1% in females (F) and 29.8% (p<0.001) in males (M). The prevalence of microorganisms was as follows: 30.3% U. parvum (F: 35.6%, M: 8.3%), 6.9% U. urealyticum (F: 6.8%, M: 7.0%), 4.9% M. hominis (F: 5.4%, M: 2.3%), 4.9% C. trachomatis (F: 3.4%, M: 11.4%), 1.1% M. genitalium (F: 1.0%, M: 1.2%), 1.2% N. gonorrhoeae (F: 0.17%, M: 5.6%) and 0.40% T. vaginalis (F: 0.38%, M: 0.53%). Mixed infections were detected in 7.4% of patients. The highest prevalence was observed for U. parvum, followed by U. urealyticum and M. hominis and a significant presence of multi-pathogen infections was registered.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122316364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic susceptibility profile of Streptococcus pneumoniae isolated from acute respiratory infection in Dakar: a cross sectional study 达喀尔急性呼吸道感染中分离的肺炎链球菌的抗生素敏感性分析:一项横断面研究

Microbiologia Medica Pub Date : 2018-12-19 DOI: 10.4081/MM.2018.7862

A. Diop, M. Camara, A. Seck, A. Dieng, A. Diop, Amary Fall, C. Diop, D. Boiro, J. Diouf, I. Sene, M. Guéye, M. Niang, C. Boye

{"title":"Antibiotic susceptibility profile of Streptococcus pneumoniae isolated from acute respiratory infection in Dakar: a cross sectional study","authors":"A. Diop, M. Camara, A. Seck, A. Dieng, A. Diop, Amary Fall, C. Diop, D. Boiro, J. Diouf, I. Sene, M. Guéye, M. Niang, C. Boye","doi":"10.4081/MM.2018.7862","DOIUrl":"https://doi.org/10.4081/MM.2018.7862","url":null,"abstract":"Streptococcus pneumoniae is a pathogen causing pneumonia, meningitis, otitis and bacteraemia. Nowadays, S. pneumoniae is developing antibacterial resistance, particularly for those with reduced susceptibility to penicillin. The objective of this study was to assess the susceptibility profile of S. pneumoniae strains isolated from acute respiratory infections (ARIs) in children younger than 5 years of age in Dakar, Senegal. S. pneumoniae strains were isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and middle ear secretion from children in the Paediatric Department of Abass Ndao University Teaching Hospital and Paediatric Department of Roi Baudouin Hospital in Dakar, Senegal. The strains were cultivated on Columbia agar supplemented with 5% of horse blood and gentamicin (6 mg/L). Antibiotic susceptibility testing was performed using E-test method. A total of 34 strains of S. pneumoniae were isolated and identified in this study, among them 7 strains (20.58%) showed penicillin-resistance. Antibiotics such as amoxicillin/clavulanic acid (MIC90=0.036 μg/mL), cefuroxim (MIC90=0.38 μg/mL), cefixim (MIC90=1.5 μg/mL), as well as macrolides (azithromycin MIC90=1.5 μg/mL, clarithromycin MIC90=0.125 μg/mL) and fluoroquinolone (levofloxacin MIC90=1 μg/mL, ofloxacin MIC90=2 μg/mL) were mostly active. However, all S. pneumoniae strains were resistant to sulfamethoxazole/trimethoprim (MIC90: 32 μg/mL). Except of S. pneumoniae strains penicillin-resistance or reduced susceptibility, most strains were susceptible to β-lactams antibiotics commonly used in ARI treatment. Continuous surveillance of antimicrobial resistance patterns of pneumococcus strains is still crucial for effective control of ARIs in children.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114587395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

La rivista che vorrei 我想要的杂志

Microbiologia Medica Pub Date : 2018-12-19 DOI: 10.4081/MM.2018.7997

E. Fiscarelli

引用次数: 0

Microbial biofilm: a “sticky” problem 微生物生物膜:一个“棘手”的问题

Microbiologia Medica Pub Date : 2018-12-19 DOI: 10.4081/mm.2018.7851

A. Pompilio, G. Bonaventura

引用次数: 3

Globalization effects on the reports of non-endemic parasitosis in Italy 全球化对意大利非地方性寄生虫病报告的影响

Microbiologia Medica Pub Date : 2018-08-19 DOI: 10.4081/MM.2018.7932

G. Bracaglia, L. Mancinelli, S. Ranno, F. Del Chierico, Hyppolite K. Tchidjou, O. Porzio, A. Onetti Muda, L. Putignani

{"title":"Globalization effects on the reports of non-endemic parasitosis in Italy","authors":"G. Bracaglia, L. Mancinelli, S. Ranno, F. Del Chierico, Hyppolite K. Tchidjou, O. Porzio, A. Onetti Muda, L. Putignani","doi":"10.4081/MM.2018.7932","DOIUrl":"https://doi.org/10.4081/MM.2018.7932","url":null,"abstract":"Protozoa and helminths are responsible for several intestinal parasite infections (IPIs). Generally, helminth infections are very unsafe but scarcely reported in high-income countries, while protozoa and helminth co-infections are usually reported in children living in inadequate hygienic-sanitary conditions and in rural areas. However, the impact of growing globalization, intense travelling, international adoptions and high levels of immigrants and refugees has significantly incremented the incidence of orofecal parasitosis in non-endemic areas. Although most IPs clear without treatment when population, even children, emigrate from endemic to different geographical areas, some IPIs such as strongyloidiasis may persist for decades as subclinical infections or as low-grade disease with nonspecific clinical manifestations, unless to reappear under impairment conditions. Herein we report an unusual case of Giardia lamblia and Trichuris spp. chronic asymptomatic co-infection in a healthy adopted Romanian child, living in a Central Italy rural area, and a hidden case of Strongyloides stercoralis in an adopted Burundian child, resident in South Italy, long misdiagnosed as a recurrent undefined dermatitis. Our report suggests the need to review primary care practitioner guidelines and children’s hospital procedures for appropriate IPIs screening and follow-up, hence providing new screening and prevention strategies, in agreement with international guidelines.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121852251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The colours of bacteria and fungi 细菌和真菌的颜色

Microbiologia Medica Pub Date : 1900-01-01 DOI: 10.4081/mm.2019.8631

E. Fiscarelli

引用次数: 0

Assessment of serum Aspergillus galactomannan expression and associated risk factors among patients living with HIV at Ilorin, Nigeria 尼日利亚伊洛林艾滋病毒感染者血清半乳甘露聚糖曲霉表达及相关危险因素评估

Microbiologia Medica Pub Date : 1900-01-01 DOI: 10.4081/mm.2019.8158

H. Adekola, O. Agbede, I. Abdullahi, L. Olayemi, A. Emeribe, A. Dangana, T. Yunusa

{"title":"Assessment of serum Aspergillus galactomannan expression and associated risk factors among patients living with HIV at Ilorin, Nigeria","authors":"H. Adekola, O. Agbede, I. Abdullahi, L. Olayemi, A. Emeribe, A. Dangana, T. Yunusa","doi":"10.4081/mm.2019.8158","DOIUrl":"https://doi.org/10.4081/mm.2019.8158","url":null,"abstract":"Invasive pulmonary aspergillosis still constitutes a diagnostic challenge for clinicians due to its non-specific symptoms. HIV infection represents one of the major risk factors due to the progressive failure of the immune system. In this study, Aspergillus serum galactomannan enzyme linked immunosorbent assay was used for the diagnosis of invasive pulmonary aspergillosis in patients living with HIV/AIDS attending University of Ilorin Teaching Hospital Antiretroviral Therapy Clinic, Nigeria. Blood samples were collected from 97 patients affected by HIV/AIDS. Age range of the study population was between 0 and 79 years. Length of the study: 3 weeks. Three out of the 97 samples were positive for the galactomannan assay with an optical density index of 1.1, 0.9 and 1.1 using the 0.5 cut-off optical density value; the concentration level of galactomannan antigen was therefore 2.20 μg/L, 1 μg/L and 2.19 μg/L, respectively. A prevalence of 3.1 % (3/97) was detected; all positive participants have been on ART for more than 3 years (P<0.0001), and 2 out of the 3 had CD4 count higher than 200 cells/mm3 (P<0.0001). Serum Aspergillus galactomannan assay is less invasive for patients and diagnosis can be made in a relatively shorter time compared to other techniques used in diagnosing invasive pulmonary aspergillosis.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128420920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1