{"title":"At “half mask” or “nose commando:” A note demonstrating nasal breaths can spread microorganisms when improperly wearing a mask during COVID-19","authors":"Robert J. Wolff","doi":"10.20944/preprints202008.0663.v1","DOIUrl":"https://doi.org/10.20944/preprints202008.0663.v1","url":null,"abstract":"Many individuals are wearing face masks improperly at ‘half mask’ and potentially breathing out microbes that can contaminate the air as well as anything below the nose. This note provides the first report that bacteria and fungi breathed out during nasal air exhalation are able to be cultured after landing on blood agar plates. The CFU’s are higher after both 10 breaths and extremely significant for 20 breaths compared to the control plates exposed to the air. Implications of this finding are that going ‘nose commando’ may be able to continue the spread of respiratory diseases such as COVID-19. Minute bioaerosols carrying bacteria may be designated as microsnot.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122271313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phages and phage therapy: past, present and future","authors":"N. Essa, Martina Rossitto, E. Fiscarelli","doi":"10.4081/mm.2020.8709","DOIUrl":"https://doi.org/10.4081/mm.2020.8709","url":null,"abstract":"Bacteriophages are viruses that infect bacteria. Since their discovery in the last century, they have proved their effectiveness in biocontrol of bacteria. In this mini-review, we provide a brief history of bacteriophages, their life cycle and classification. We also discuss the potential use of bacteriophage in clinical therapy as an alternative to antibiotics, comparing their advantages and disadvantages.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114210723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prospective nucleotide sequence analysis of methicillin resistant Staphylococcus aureus isolates from Sokoto state","authors":"S. Adeiza, J. Onaolapo, B. Olayinka","doi":"10.4081/mm.2020.8496","DOIUrl":"https://doi.org/10.4081/mm.2020.8496","url":null,"abstract":"The epidemiology of MRSA has evolved considerably. In the past, MRSA strains could be traced back to a hospital or clinic source, but over the years there appear to be a decrease in hospital acquired MRSA and an upsurge in the occurrence in community acquired strains. Even-though in Nigeria there are studies that have reported on the molecular characterization of MRSA, Sadly, not much effort has been put into analysis and submission of sequenced data to Gene repositories. Based on information available to us, this is the first study to have successfully deposited nucleotide sequences of MRSA strains from Sokoto state owned Hospital into the Genbank database. Sequence reads of Molecular characterization should be deposited into Gene repositories database by researchers to further populate the database and improve the efficiency of local phylogenetic analysis to foster better bioinformatics as paucity of local data exists in these databases.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129975458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. D'Aleo, V. Gatto, Mattia Chisari, D. Marcuccio, M. Cuzzola, M. Conte, C. Farina
{"title":"First utilization of BioFire FilmArray® Respiratory Panel 2 Plus in post-mortem diagnosis of Influenza A H1N1","authors":"F. D'Aleo, V. Gatto, Mattia Chisari, D. Marcuccio, M. Cuzzola, M. Conte, C. Farina","doi":"10.4081/mm.2019.8311","DOIUrl":"https://doi.org/10.4081/mm.2019.8311","url":null,"abstract":"Post-mortem microbiology is an important tool in legal medicine and forensic pathology; this case report shows the importance of microbiological testing to establish the cause of death in infection suspected death. We refer a case of fatal Influenza A (H1N1) diagnosed only after dead with post-mortem microbiology tool.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116967875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First case of fungal rhinosinusitis due to Aspergillus nomius in a child with aplastic anaemia","authors":"Y. Oz, Müge Aslan, Nuray Gundogdu, Özcan Bör","doi":"10.4081/mm.2019.8148","DOIUrl":"https://doi.org/10.4081/mm.2019.8148","url":null,"abstract":"Recently, infections caused by Aspergillus species have increased dramatically. Invasive aspergillosis (IA) is one of the most important causes of morbidity and mortality in immunocompromised patients, such as those with haematological malignancies who undergo chemotherapy, bone marrow and solid organ transplant recipients, and patients with other immunodeficiency. The most common species causing invasive infections include Aspergillus fumigatus, followed by Aspergillus flavus. Aspergillus nomius is an anamorphic species belonging to Aspergillus section Flavi, which currently include 22 species that can be grouped into seven clades (Aspergillus flavus, Aspergillus tamarii, Aspergillus nomius, Petromyces alliaceus, Aspergillus togoensis, Aspergillus leporis and Aspergillus avenaceus) based on morphological characters, sequence data, and extrolite profiles. These species may also produce toxic and carcinogenic aflatoxins. However, Aspergillus nomius is an emerging pathogen as a cause of IA; we found only two reported cases of invasive infection caused by this fungus in literature up till now. We reported a case of fungal rhinosinusitis caused by Aspergillus nomius in a child with aplastic anaemia and to our knowledge, it is the first case as an agent of rhinosinusitis. The isolate was identified by sequencing based methods.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128367788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reduction of response time for the assessment of invasive bacterial disease in bacteriology by using h24 molecular tests at low operational impact","authors":"N. Corbo, C. Comi","doi":"10.4081/mm.2019.8363","DOIUrl":"https://doi.org/10.4081/mm.2019.8363","url":null,"abstract":"In February 2017, the Lombardy Region (Italy) provided some indications for the surveillance of invasive bacterial diseases, including the need to perform molecular tests together with culture and other diagnostic tests. Starting from September 2017, the Microbiology and Virology Unit of ASST Lecco has acquired the FilmArray system (BioMérieux, Marcy-l’Étoile, France) which includes the Meningitis/Encephalitis (ME) panel, able to identify 14 common pathogens responsible for community acquired meningitis or encephalitis including virus, bacteria and yeast with molecular technology directly from cerebrospinal fluid (CFS). In 2018, the organizational model implemented has provided to use of the FilmArray system by the TSLB dedicated to microbiology during routine hours (8.00-16.30) and by the TSLB of Clinical Pathology on active guard from 16.30 to 8.00 with validation of the analysis by the Manager available. The new organization, which involved the use of the FilmArray ME h 24 panel by the TSLB who works belonging to Clinical Pathology and Microbiology and Virology, has allowed a considerable reduction of response times in case of suspected meningitis.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131734955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Granero, Daniela Dompé, E. Peyronel, C. Crocillà, M. Cavallo
{"title":"An unexpected case report of Ascaris lumbricoides in pregnant woman","authors":"V. Granero, Daniela Dompé, E. Peyronel, C. Crocillà, M. Cavallo","doi":"10.4081/MM.2019.7585","DOIUrl":"https://doi.org/10.4081/MM.2019.7585","url":null,"abstract":"Soil-transmitted helminths, primarily Ascaris, Trichuris and hookworm, infect more than 760 million people worldwide. Parasitic diseases represent a social and economic problem in developing countries. Herein, we present a case of ascariasis in a pregnant woman, who was treated with mebendazole for three days without teratogenic effects. It is emphasized the need not to underestimate helminth infections in developed countries and how rapid notification is needed in order to quickly establish a guided therapy especially in pregnant women.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117063367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The global Mycobacterium chimaera outbreak","authors":"E. Tortoli","doi":"10.4081/MM.2019.8037","DOIUrl":"https://doi.org/10.4081/MM.2019.8037","url":null,"abstract":"Infections due to Mycobacterium chimaera associated with a heater-cooler device used during the open-heart surgery have been reported worldwide. These cases represent the first outbreak caused by a non-tuberculous mycobacterium at a global level. Salient features of the infection are long latency and high mortality. Different aspects of the M. chimaera outbreak, from the first detected cases to the present burden are reviewed in this study. Although the source has been found and thwarted, new cases continue to be detected due to the late manifestation of symptomatology. The treatment remains poorly effective and mortality is around 50%.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114952011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The journal I would like","authors":"E. Fiscarelli","doi":"10.4081/MM.2019.8203","DOIUrl":"https://doi.org/10.4081/MM.2019.8203","url":null,"abstract":"Not available","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"228 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126568652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Mariano, A. Salzo, V. Felice, Massimiliano Scutellà
{"title":"Atopobium vaginae: a literature review, on findings and potential clinical implications","authors":"A. Mariano, A. Salzo, V. Felice, Massimiliano Scutellà","doi":"10.4081/MM.2019.8014","DOIUrl":"https://doi.org/10.4081/MM.2019.8014","url":null,"abstract":"Atopobium vaginaeis an anaerobic Gram-positive bacterium. It is recognized as a causal agent of bacterial vaginosis onset and occasionally associated to gynaecologic-obstetric complications (including pelvic inflammatory disease, endometritis, tubo-ovarian abscesses and preterm delivery with neonatal consequences). Additionally, bacteraemia and invasive infections have rarely been reported. The scientific and technological progress allowed an accurate and rapid identification of Atopobium vaginae supporting diagnosis and clinic interventions. In this article, literature has been deeply examined to report the state-of-the-art on Atopobium vaginae, which might be useful in clinical scenarios.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"207 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122357599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}