Microbiologia Medica最新文献

A rare case of pneumococcal keratitis in a patient with Herpes Zoster Ophthalmicus and compromised immune status 带状疱疹眼炎患者和免疫力低下者患肺炎球菌角膜炎的罕见病例

Microbiologia Medica Pub Date : 2024-05-17 DOI: 10.4081/mm.2024.11676

Dibyashree Dibyashree, N. Goyal, Rekha Yadav, Narendra Pal Singh, Kalyani Swain, Vikas Manchanda, Poonam Singh

{"title":"A rare case of pneumococcal keratitis in a patient with Herpes Zoster Ophthalmicus and compromised immune status","authors":"Dibyashree Dibyashree, N. Goyal, Rekha Yadav, Narendra Pal Singh, Kalyani Swain, Vikas Manchanda, Poonam Singh","doi":"10.4081/mm.2024.11676","DOIUrl":"https://doi.org/10.4081/mm.2024.11676","url":null,"abstract":"Infectious keratitis is commonly encountered in the ophthalmology emergency department. Streptococcus pneumoniae causing bacterial keratitis is a cause of concern in developing countries, owing to its complication of irreversible corneal scarring. This is a rare case report of a 25-year-old immunocompromised patient who presented to the Ophthalmology Department in a tertiary care hospital in India with complaints of diminution of vision in the left eye with pain, redness and watering, along with a history of vesicular painful rashes involving the left side of the forehead, the tip of the nose and the supraciliary area. Gram-positive lanceolate diplococci and plenty of pus cells were seen on the Gram staining of the corneal abscess scrape. The clinical presentation of the patient led to a provisional clinical diagnosis of secondary bacterial infection in Herpes Zoster Opthalmicus (HZO), corneal abscess with herpetic keratitis. Quellung reaction yielded an 18C serotype of the isolated pneumococci. Prompt treatment was required to prevent rapidly progressive complications.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"56 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140965488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Brucella (Ochrobactrum) anthropi by MALDI-TOF MS from blood: a case report 通过 MALDI-TOF MS 从血液中鉴定炭疽布鲁氏菌：一份病例报告

Microbiologia Medica Pub Date : 2024-03-12 DOI: 10.4081/mm.2024.11885

M. T. Della Rocca, Filomena Merola, Adriana Durante, V. Panetta, Stefano Labella, Giuseppina Tucci, Antonio Marino, Giusy Natale, Francesco Pardo, Catia Sabbato, Rita Greco

引用次数: 0

First reported case of Phoma multirostrata from central India 印度中部首次报告的多层噬菌体病例

Microbiologia Medica Pub Date : 2024-03-11 DOI: 10.4081/mm.2024.12199

Ravita Sharma, N. Channe, Shilpa Pandhare, Varsha Wanjare, Dr. Neha Sharma, Sunanda Shrikhande

{"title":"First reported case of Phoma multirostrata from central India","authors":"Ravita Sharma, N. Channe, Shilpa Pandhare, Varsha Wanjare, Dr. Neha Sharma, Sunanda Shrikhande","doi":"10.4081/mm.2024.12199","DOIUrl":"https://doi.org/10.4081/mm.2024.12199","url":null,"abstract":"A 50-year-old male patient, resident of Gondia, Maharashtra, India, presented with chief complaints of swelling and raw areas over left foot for 3 months. Patient was apparently alright 3 months prior when he had a traumatic injury with a piece of wood which went across his whole foot, which was removed by patient himself, and then he visited a private hospital where he was given various medications, which gave him partial relief. After a few days, the patient developed a painful swelling over the left foot, up to the ankle, which was associated with difficulty in walking and local rise of temperature, and not with fever or other symptoms. Following this, after a month, the patient developed a single raised lesion, which progressed to involve the whole left foot over a period of 5 days. The lesions then burst spontaneously, which was associated with bloody discharge. For these complaints, the patient visited a nearby hospital, where he was admitted and given various medications, including tablet amoxycillinpotassium clavulanate 625 mg, tablet pantoprazole 40 mg, tablet paracetamol 500 mg and fusidic acid cream application, which gave him partial relief. After a few days, the patient developed a burning sensation over these lesions, for which he was then referred to GMC, Nagpur, Maharashtra, India, from same hospital.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140251316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative evaluation of colistin Minimum Inhibitory Concentration determination by reference broth microdilution with other commonly used phenotypic methods in Multidrug-Resistant Gram-negative bacilli 用肉汤微量稀释法测定耐多药革兰氏阴性杆菌中可乐定最低抑制浓度与其他常用表型法的比较评估

Microbiologia Medica Pub Date : 2023-12-07 DOI: 10.4081/mm.2023.11573

N. Goyal, Seema Gangar, Malika Grover, Narendra Pal Singh, Aditya Nath Dwivedi, Ananya Varshney, Deeksha Chaudhary, Neelam Arya

{"title":"A comparative evaluation of colistin Minimum Inhibitory Concentration determination by reference broth microdilution with other commonly used phenotypic methods in Multidrug-Resistant Gram-negative bacilli","authors":"N. Goyal, Seema Gangar, Malika Grover, Narendra Pal Singh, Aditya Nath Dwivedi, Ananya Varshney, Deeksha Chaudhary, Neelam Arya","doi":"10.4081/mm.2023.11573","DOIUrl":"https://doi.org/10.4081/mm.2023.11573","url":null,"abstract":"Background: over the past decade, the dependency on colistin as a last resort antibiotic has driven the global emergence of colistin resistance among many bacterial species. This study comparatively evaluated the colistin Minimum Inhibitory Concentration (MIC) by various phenotypic methods, including the reference method of Broth Microdilution (BMD), other approved methods of Colistin Broth Disk Elution (CBDE), and Colistin Agar Test (CAT) and widely available method of Epsilometer Test (E-test) among Multidrug Resistant (MDR) Gram-negative bacteria. Methods: ninety Gram-negative bacterial isolates that were resistant to three or more classes of drugs (MDR) were included in the study. All the MDR isolates were subjected to colistin susceptibility determination by BMD, CBDE, CAT, and E-test. Results: amongst 1118 samples, 90 (8.05%) samples yielded MDR Gram-negative bacilli. All the MDR Gram-negative isolates were colistin intermediate by all four methods of phenotypic colistin susceptibility. Three Acinetobacter baumannii and two Klebsiella pneumoniae isolates that had MIC of 2 μg/mL by BMD, displayed MIC of <1 μg/mL by CBDE and CAT. Three isolates (2 Citrobacter koseri and 1 Enterobacter spp.) showed higher MIC by the E-strip method in comparison to BMD. Conclusions: our study holds significance, as there is a paucity of data comparing the four phenotypic methods for colistin MIC determination; BMD is the most reliable, gold standard method, but it is labor-intensive and requires technical expertise. In the present study, CBDE and CAT methods showed good concordance with BMD, and are easy to perform with limited logistics. Thus, they can be used as an alternative to BMD. We found that even though the Etest method was less accurate, it can still be used with caution to exclude the possibility of colistin resistance.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"32 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138592260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patterns of Mannose-Binding Lectin (MBL) responses to Plasmodium falciparum infections in hyperendemic settings 高流行病环境中恶性疟原虫感染的甘露糖结合凝集素（MBL）反应模式

Microbiologia Medica Pub Date : 2023-12-07 DOI: 10.4081/mm.2023.11517

Dia Aldeen Alfaki, M. Hussein, Amanda Elgoraish, M. M. Elbasheir

{"title":"Patterns of Mannose-Binding Lectin (MBL) responses to Plasmodium falciparum infections in hyperendemic settings","authors":"Dia Aldeen Alfaki, M. Hussein, Amanda Elgoraish, M. M. Elbasheir","doi":"10.4081/mm.2023.11517","DOIUrl":"https://doi.org/10.4081/mm.2023.11517","url":null,"abstract":"Background: malaria caused by Plasmodium falciparum remains a significant and major public health concern in Africa, particularly in hyperendemic regions. Recurrent fevers and high quantities of inflammatory mediators in the circulation define the disease's blood stages. By binding to non-self-pathogen oligosaccharide surfaces, Mannose-Binding Lectin (MBL) and lectin complement pathways trigger innate immune processes and contribute to the formation of adaptive immune responses. Methods: in Sudan, we investigated the varied immune response levels of MBL to the different phases of P. falciparum infection in White Nile and Blue Nile states endemic to malaria. And we looked at the interaction of regulatory Interleukin 6 (IL- 6) cytokines on MBL during infection. Our study was based on a total of 108 cases, in which 86 patients (62.0%) were uncomplicated and (17.6%) were severe, all met the diagnostic criteria and were clinically admitted for malaria infections. For the determination of serum MBL and IL-6 levels, a commercial ELISA kit was employed. Results: the analysis of the results revealed significantly elevated levels of MBL and IL-6 in both severe and uncomplicated cases (p<0.001). And MBL average in contrast to residents, Blue Nile patients had high parasitemia (599.9 ng/mL) and this difference was statistically significant (p-value<0.05). The remarkable positive correlation of IL-6 serum levels with MBL among malaria patients and healthy controls (r=0.399, p<0.001) was noted too. Conclusions: according to the findings of this study, patients living in hyperendemic areas exhibit a different MBL response rate and appear to be more homogeneous in proportion to the density of P. falciparum due to parasitemia. In addition, it is also dependent on the regulatory immune mediator IL-6.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"41 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138594062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria 选定消毒剂对尼日利亚拉各斯州金黄色葡萄球菌生物膜形成临床分离物的影响

Microbiologia Medica Pub Date : 2023-12-04 DOI: 10.4081/mm.2023.11445

U. Essiet, A. Ajayi, A. Adeleye, S. I. Smith

{"title":"Effect of selected disinfectants on biofilm-forming clinical isolates of Staphylococcus aureus in Lagos State, Nigeria","authors":"U. Essiet, A. Ajayi, A. Adeleye, S. I. Smith","doi":"10.4081/mm.2023.11445","DOIUrl":"https://doi.org/10.4081/mm.2023.11445","url":null,"abstract":"Background and Aims: Staphylococcus aureus is one of the most important pathogens of public health concern and a leading cause of nosocomial infections. In this study, we evaluated the effect of routinely used disinfectants in hospitals for surface decontamination on biofilm-forming S. aureus.\u0000Materials and Methods: forty-eight S. aureus isolates were phenotypically evaluated for biofilm formation using the Tissue Culture Plate (TCP) technique. Effect of disinfectants (Dettol®, Izal®, Jik® and Savlon®) on biofilm was tested and time-kill kinetics evaluated. PCR was used to confirm the identity of S. aureus using species-specific primers.\u0000Results: biofilm formation assay revealed that 15 (31.2%) of the isolates formed biofilm with 7 (14.5%) and 8 (16.6%) considered as strong and moderate biofilm formers, respectively. Biofilm formation was time-dependent (p<0.0001). Jik® was significantly effective (p<0.0001) as it disrupted biofilm formed in all 15 (100%) isolates, followed by Izal® 13 (86.6%), Savlon® 11 (73.3%) and Dettol® 9 (60%). Time-kill kinetics of the four disinfectants revealed Dettol®, Jik® and Savlon® achieved total (100%), (7 log10) lethality against isolates within 1 h contact time while Izal® attained complete lethality at 6 h contact time.\u0000Conclusions: of the four disinfectants evaluated Jik®, a chlorine- based formulation, was more effective in destroying biofilmforming S. aureus. The need to use effective disinfectants in sanitization is imperative to facilitate the control and prevention of hospital and community-acquired infections.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"12 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138604069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Covid causes of acute undifferentiated febrile illness during the Covid pandemic: an etiological analysis from Uttar Pradesh, India Covid大流行期间急性无分化发热性疾病的非Covid病因:来自印度北方邦的病因学分析

Microbiologia Medica Pub Date : 2023-08-22 DOI: 10.4081/mm.2023.11092

Prof Vikas Kumar, S. Saraswat, Pranay Singh, A. Goyal, Sanjeev Kumar, Madhav Agrawal, H. Kumar

{"title":"Non-Covid causes of acute undifferentiated febrile illness during the Covid pandemic: an etiological analysis from Uttar Pradesh, India","authors":"Prof Vikas Kumar, S. Saraswat, Pranay Singh, A. Goyal, Sanjeev Kumar, Madhav Agrawal, H. Kumar","doi":"10.4081/mm.2023.11092","DOIUrl":"https://doi.org/10.4081/mm.2023.11092","url":null,"abstract":"Background and Aims: febrile illnesses are one of the leading causes of morbidity and mortality in India, which are very common in the monsoon and post-monsoon season in tropical countries. Acute Undifferentiated Febrile Illness (AUFI) is a term usually used to refer to such conditions until diagnosed. This study was conducted to understand the prevalence of mixed infections, and the etiology and seasonal distribution of AUFI cases during the Corona Virus Disease (COVID) pandemic. Materials and Methods: this study was a hospital-based crosssectional study of six months (August 2021 to January 2022). Samples were collected by random sampling method from SN Medical College, Agra, and Mathura District. The diagnosis was made by Rapid Diagnostic Test for Malaria, and ELISA for Dengue, Chikungunya, Leptospira, and Scrub typhus. Results: a total of 9016 non-repetitive serum samples were collected, from males (4657) and females (4359), with a mean age of 42 years. The most common infections were: dengue (26.5%), malaria (0.85%), leptospira (0.54%), scrub typhus (0.32%), and Chikungunya (0.14%). The commonest co-infection was dengue with scrub typhus. Triple infections were also observed. Conclusions: the diversity of clinical presentations and etiological agents with limited diagnostic facilities demonstrates the complexity of AUFI. The knowledge of the local and seasonal distribution of acute febrile illnesses is thus very useful to formulate clinical, diagnostic, and management algorithms for positive outcomes, reducing hospital costs, and burden on healthcare facilities. Further upliftment of health services at the root level is still a long way to go.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121797708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterococcus casseliflavus bacteremic infection of a right atrial thrombotic mass: first reported case 右心房血栓性肿块的干酪黄肠球菌菌血症感染:首例报道

Microbiologia Medica Pub Date : 2023-08-11 DOI: 10.4081/mm.2023.10982

A. Mastroianni, S. Greco, V. Vangeli, M. Mauro, Francesca Greco, R. Manfredi

引用次数: 0

Comparison of the Etest and Sensititre methods for anaerobe susceptibility testing 厌氧药敏试验的est和Sensititre方法的比较

Microbiologia Medica Pub Date : 2023-07-28 DOI: 10.4081/mm.2023.11056

K. Puechler, Katia Scalzo, Maira Nicoletti, S. Wieser, R. Aschbacher, E. Pagani

{"title":"Comparison of the Etest and Sensititre methods for anaerobe susceptibility testing","authors":"K. Puechler, Katia Scalzo, Maira Nicoletti, S. Wieser, R. Aschbacher, E. Pagani","doi":"10.4081/mm.2023.11056","DOIUrl":"https://doi.org/10.4081/mm.2023.11056","url":null,"abstract":"Background and Aims: Antimicrobial susceptibility testing of anaerobic clinical isolates is of paramount importance for patient therapy and resistance monitoring. In our laboratory the MIC gradient Etest method and broth microdilution with Sensititre trays are used for susceptibility testing of anaerobes and the aim of this study was to compare the two methods on a panel of anaerobes routinely isolated from patients in the province of Bolzano, Italy. Materials and Methods: Totally, 74 non-repetitive Gram-positive and Gram-negative patient isolates were tested with Etest strips on Fastidious Anaerobe Agar (F.A.A.) and with Sensititre trays, according to manufacturer’s instructions. Interpretation of MICs was by EUCAST or CLSI criteria, resistance percentages were calculated and Categorical Agreement (CA) and Essential Agreement (EA) between the two methods were determined. Results: Of the 74 isolates, 68 (91.9%) grew on both systems and agreement for these was compared in the study. CA for all isolates was ≥90% for all tested antibiotics except moxifloxacin, whereas EA was generally lower. Resistance was generally low, except for clindamycin in all isolates and tigecycline in Gram-negatives. In our study Etest was a superior and more handy method. Conclusions: To conclude, we believe the Etest method is more suitable for routine diagnostic laboratory usage. Nevertheless, multicenter studies are required to evaluate the two methods for anaerobic susceptibility testing.","PeriodicalId":422034,"journal":{"name":"Microbiologia Medica","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127415687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Klebsiella pneumoniae ipervirulenta: una nuova emergenza? L’esperienza della Fondazione IRCCS Policlinico San Matteo di Pavia 高毒性肺炎:新的紧急情况?IRCCS polilinico San Matteo di Pavia基金会的经验

Microbiologia Medica Pub Date : 2023-04-11 DOI: 10.4081/mm.2023.11375

引用次数: 0