Science BulletinPub Date : 2025-04-28DOI: 10.1016/j.scib.2025.04.053
Lina Du, Rigui Ye, Xin Liu, Qianqian He, Jiyue Qiao, Laurence Charrier, Markus Geisler, Zhenyu Gao, Qian Qian, Yanhua Qi
{"title":"The OsbHLH166-OsABCB4 module regulates grain length and weight via altering auxin efflux.","authors":"Lina Du, Rigui Ye, Xin Liu, Qianqian He, Jiyue Qiao, Laurence Charrier, Markus Geisler, Zhenyu Gao, Qian Qian, Yanhua Qi","doi":"10.1016/j.scib.2025.04.053","DOIUrl":"https://doi.org/10.1016/j.scib.2025.04.053","url":null,"abstract":"<p><p>Grain size is one of the key factors determining grain weight and yield. However, it remains largely unknown how the auxin signal regulates grain size in rice. Here, a quantitative trait locus qGL1 for grain length was identified with recombinant inbred lines 9311 and Nipponbare (NIP), and fine-mapped to the OsABCB4 gene, which encodes a member of the ATP Binding Cassette B (ABCB) subfamily. Compared to NIP, loss of OsABCB4 function leads to longer and heavier grains, while over-expression of OsABCB4 causes shorter and lighter grains, demonstrating a negative control of grain length and weight in rice. Haplotype analyses using 3024 rice-sequenced genomes indicated that SNP<sub>25</sub> and SNP<sub>55</sub> in OsABCB4 are correlated with grain length in rice. OsbHLH166, a basic helix-loop-helix transcription factor, was screened from a yeast one-hybrid library and confirmed to directly bind the promoter of OsABCB4 by electrophoretic mobility shift assay and yeast one hybridization validation and to activate the expression of OsABCB4 by dual-luciferase reporter assay. And OsbHLH166 expressions matching OsABCB4 were all higher expressed in stems and glumes. OsABCB4 was subcellularly localized on the plasma membrane, which was verified as an auxin efflux transporter. Histological analysis, IAA content measurement and DR5:GUS activity analyses demonstrated that OsbHLH166 and OsABCB4 control grain length by regulating cell expansion and auxin contents in glume cells and altering the expression of GL7, GS2, TGW3 and GS3 genes during rice grain development. This newly identified OsbHLH166-OsABCB4 module sheds light on our understanding of molecular mechanisms underlying the regulation of grain size and weight via auxin and provides a new gene resource for molecular design breeding of grain type.</p>","PeriodicalId":421,"journal":{"name":"Science Bulletin","volume":" ","pages":""},"PeriodicalIF":18.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science BulletinPub Date : 2025-04-24DOI: 10.1016/j.scib.2025.04.047
Huiru Zhong, Lu Shen, Meng Qu, Yang Ou, Yuzhong Zhang, Huiqin Mao
{"title":"Quantifying methane emission baselines with high-resolution satellite data to support China's emission control.","authors":"Huiru Zhong, Lu Shen, Meng Qu, Yang Ou, Yuzhong Zhang, Huiqin Mao","doi":"10.1016/j.scib.2025.04.047","DOIUrl":"https://doi.org/10.1016/j.scib.2025.04.047","url":null,"abstract":"","PeriodicalId":421,"journal":{"name":"Science Bulletin","volume":" ","pages":""},"PeriodicalIF":18.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science BulletinPub Date : 2025-04-24DOI: 10.1016/j.scib.2025.04.050
Yong Ge, Li Zhang, Yi Han, Die Zhang, Wenjing Yang, Lian Feng, Jinwei Dong, Shuping Wang, Simin Peng, Chaoyang Fang
{"title":"Enhancing water security in lake basins through geographic intelligence technology.","authors":"Yong Ge, Li Zhang, Yi Han, Die Zhang, Wenjing Yang, Lian Feng, Jinwei Dong, Shuping Wang, Simin Peng, Chaoyang Fang","doi":"10.1016/j.scib.2025.04.050","DOIUrl":"https://doi.org/10.1016/j.scib.2025.04.050","url":null,"abstract":"","PeriodicalId":421,"journal":{"name":"Science Bulletin","volume":" ","pages":""},"PeriodicalIF":18.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Science BulletinPub Date : 2025-04-21DOI: 10.1016/j.scib.2025.04.039
Wanguo Gao, Zhe Lu, Yecheng Leng, Chengyang Wu, Yingfang Yao, Xi Zhu, Junchuan Sun, Xiwen Yu, Cheng Wang, Congping Wu, Wenlei Wu, Bing Wang, Lu Wang, Zhigang Zou
{"title":"Phonon resonance enabled Cu(I) valence pinning in hydroxyapatite for photothermal CO<sub>2</sub> hydrogenation.","authors":"Wanguo Gao, Zhe Lu, Yecheng Leng, Chengyang Wu, Yingfang Yao, Xi Zhu, Junchuan Sun, Xiwen Yu, Cheng Wang, Congping Wu, Wenlei Wu, Bing Wang, Lu Wang, Zhigang Zou","doi":"10.1016/j.scib.2025.04.039","DOIUrl":"https://doi.org/10.1016/j.scib.2025.04.039","url":null,"abstract":"<p><p>Cu-based catalysts attract considerable attention because of their exceptional CO<sub>2</sub> photo-/electro-/thermo-reduction capacities, where Cu(I) is generally treated as the active species. However, a significant problem hindering the large-scale applications of Cu-based catalysts is the inactivation of Cu(I) via irreversible redox to Cu(II)/Cu(0). This study proposes a Cu(I) valence pinning method based on hydroxyapatite (HAP). Experimental and theoretical studies demonstrate that the phonon resonance among the Cu(I) ions, their adjacent heteroatoms, and the intermediates adsorbed at the Cu(I) sites yields Cu(I) valence electrons in their lowest energy states. Thus, Cu(I) is stabilized, stable, and efficient photothermal CO<sub>2</sub> hydrogenation is promoted. However, because of the change of Cu(I) coordination environment during the CO<sub>2</sub> hydrogenation reaction, Cu(I) ions migrate into the bulk phase, leading to activity attenuation. Nevertheless, Cu(I) ions can be pulled out to the surface of HAP under the oxidative humid air condition, and the catalytic activity can be easily recovered. Thus, we propose a simple cyclic reaction/regeneration process. This enables the Ca<sub>5</sub>(FeCuCe)<sub>5</sub>-HAP catalyst to achieve the CO yield of 402.8 mmol g<sup>-1</sup> h<sup>-1</sup> and a CO<sub>2</sub> conversion rate of 27.7%, which is close to the thermodynamic equilibrium. This catalyst also displays a selectivity of approximately 100% and cycle stability of 156 h at 500 °C under a pressure of 1 atmospheric. Our study provides a viable method for the scale applications of Cu(I) based catalysts in the \"negative carbon\" industries.</p>","PeriodicalId":421,"journal":{"name":"Science Bulletin","volume":" ","pages":""},"PeriodicalIF":18.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}