The Journal of Reproduction and Development最新文献_第5页

Cellular localization, expression and functional implications of the utero-placental endothelin system during maintenance and termination of canine gestation 犬妊娠维持和终止过程中子宫胎盘内皮素系统的细胞定位、表达和功能意义

The Journal of Reproduction and Development Pub Date : 2017-02-20 DOI: 10.1262/jrd.2016-165

A. Gram, A. Boos, M. Kowalewski

{"title":"Cellular localization, expression and functional implications of the utero-placental endothelin system during maintenance and termination of canine gestation","authors":"A. Gram, A. Boos, M. Kowalewski","doi":"10.1262/jrd.2016-165","DOIUrl":"https://doi.org/10.1262/jrd.2016-165","url":null,"abstract":"Utero-placental (Ut-Pl) angiogenesis and blood flow are fundamental for successful outcome of pregnancy. They are controlled by numerous vasodilator and vasoconstrictor systems such as endothelins (EDNs) and the renin angiotensin system. Dogs possess an invasive type of placentation, classified as endotheliochorial. Despite increasing knowledge regarding canine Ut-Pl function, little information exists on uterine and placental vascular activity during initiation, maintenance and termination of pregnancy in this species. The current study investigated expression of EDNs and their receptors (EDNRA and EDNRB) in the pre-implantation uterus and Ut-Pl compartments during gestation and at normal parturition, as well as in mid-pregnant dogs treated with the antigestagen aglepristone. The Ut-Pl mRNA expression of EDN1 and EDNRA was constant until mid-gestation and increased significantly during prepartum luteolysis. In contrast, EDN2 was highest pre-implantation and decreased following placentation, remaining low thereafter. Expression of the EDN-activating enzyme ECE1 and mRNA of EDNRB increased towards mid-gestation and was further elevated at prepartum luteolysis. Antigestagen treatment resulted in increased levels of EDN1 and EDNRA. At the cellular level, the uterine expression of EDN1, ECE1 and EDNRB was found predominantly in the endometrial surface and glandular epithelial cells; uterine signals for EDNRA were weak. In Ut-Pl all targets were mainly localized in the placenta fetalis, with syncytiotrophoblast staining stronger for ECE1 and EDNRB. In contrast, EDNRA stained strongly at the base of the placental labyrinth. Expression and localization of EDNs (EDN1, -2), EDN receptors and ECE1 in the placenta fetalis suggests their involvement in the trophoblast invasion and proliferation.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126225989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Decrease in body surface temperature before parturition in ewes 母羊出生前体表温度下降

The Journal of Reproduction and Development Pub Date : 2017-02-05 DOI: 10.1262/jrd.2016-097

H. Nabenishi, A. Yamazaki

{"title":"Decrease in body surface temperature before parturition in ewes","authors":"H. Nabenishi, A. Yamazaki","doi":"10.1262/jrd.2016-097","DOIUrl":"https://doi.org/10.1262/jrd.2016-097","url":null,"abstract":"This study investigated the correlation between the body surface temperature (BST) and core body temperature of ewes and changes in BST during the prepartum stage in pregnant ewes. Four non-pregnant adult ewes were used in the first experiment. The BST of the upper neck, vaginal temperature (VT), and ambient temperature (AT) were measured every 10 min for seven days and analyzed for correlations. The mean (± SD) BST and VT of ewes during the study period were 35.4 ± 1.7°C and 39.1 ± 0.4°C, respectively, with a correlation of r = 0.62, P < 0.001. This finding suggested that the BST was associated with core body temperature in ewes. In the subsequent experiment, seven pregnant ewes in their third trimester were used to evaluate changes in BST measured at the upper neck 72 h before parturition. The mean BST at –24–0 h (0 h = time of parturition) was significantly lower than that at –72– –48 h and –48– –24 h (P < 0.05). The BST tended to decrease toward parturition; all BST measurements at –16– –3 h were significantly lower than those at –72 h (P < 0.05). A clear circadian rhythm in the BST was observed at two days and the day before parturition and an unclear circadian rhythm was observed on the day of parturition. Therefore, these findings indicate that the BST also decreases before parturition, as do vaginal and rectal temperatures.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117313901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Significant heparin effect on bovine embryo development during sexed in vitro fertilization 肝素对牛体外受精胚胎发育的影响

The Journal of Reproduction and Development Pub Date : 2017-02-05 DOI: 10.1262/jrd.2016-142

L. An, S. Chaubal, Yanhong Liu, Yexiang Chen, T. Nedambale, Jie Xu, F. Xue, J. Moreno, Shenghong Tao, G. Presicce, F. Du

{"title":"Significant heparin effect on bovine embryo development during sexed in vitro fertilization","authors":"L. An, S. Chaubal, Yanhong Liu, Yexiang Chen, T. Nedambale, Jie Xu, F. Xue, J. Moreno, Shenghong Tao, G. Presicce, F. Du","doi":"10.1262/jrd.2016-142","DOIUrl":"https://doi.org/10.1262/jrd.2016-142","url":null,"abstract":"The aim of this study was to investigate the effect of different heparin concentrations in the course of sexed in vitro fertilization (IVF), on bovine embryonic development and development to term following embryo transfer (ET). With a total of 9156 oocytes for IVF, sorted as well as unsorted sperm from four bulls had different heparin requirements for achieving the highest rate of development in vitro. However, when optimal heparin concentrations were used (40 to 80 µg/ml), the performance of X-sorted sperm (0.3 × 106/ml/IVF droplet) from all four bulls, as judged by blastocyst development (Bulls A, B, C, and D: 25.2, 19.7, 25.1, and 9.8%, respectively), was significantly increased, and the blastocyst rate was comparable to that observed with unsorted sperm at certain heparin concentrations within the four bulls. We determined that near-optimal blastocyst development was possible with sorted sperm from all four bulls, when a heparin concentration of 40 µg/ml was used. Pregnancy rates at d 70 post ET ranged from 39.1 to 40.3% (P > 0.05), and the calving rates ranged from 34.4 to 35.1% (P > 0.05), when heparin was used at a concentration of 10 μg/ml (n = 236), 20 μg/ml (n = 189), and 40 μg/ml (n = 305), respectively. Our study demonstrates that, although the sorted sperm of different bulls performed optimally over a range of heparin concentrations, a generally accepted heparin concentration of 40 µg/ml can be set for sexed IVF. This improvement is beneficial when sexed embryo production by ovum pickup and IVF is an essential component of genetic breeding programs.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121861767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Effects of exosome-like vesicles on cumulus expansion in pigs in vitro 外泌体样囊泡对猪体外积云扩张的影响

The Journal of Reproduction and Development Pub Date : 2017-02-01 DOI: 10.1262/jrd.2016-124

Yuta Matsuno, Asuka Onuma, Yoshie A Fujioka, K. Yasuhara, W. Fujii, K. Naito, K. Sugiura

{"title":"Effects of exosome-like vesicles on cumulus expansion in pigs in vitro","authors":"Yuta Matsuno, Asuka Onuma, Yoshie A Fujioka, K. Yasuhara, W. Fujii, K. Naito, K. Sugiura","doi":"10.1262/jrd.2016-124","DOIUrl":"https://doi.org/10.1262/jrd.2016-124","url":null,"abstract":"Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"64 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130937444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Positive correlations of age and parity with plasma anti-Müllerian hormone concentrations in Japanese Black cows 日本黑牛年龄和胎次与血浆抗<s:1>勒氏杆菌激素浓度呈正相关

The Journal of Reproduction and Development Pub Date : 2017-01-27 DOI: 10.1262/jrd.2016-088

Motoya Koizumi, H. Kadokawa

引用次数: 16

Establishment of DNA methylation patterns of the Fibrillin1 (FBN1) gene in porcine embryos and tissues 猪胚胎和组织中纤原蛋白1 (FBN1)基因DNA甲基化模式的建立

The Journal of Reproduction and Development Pub Date : 2017-01-22 DOI: 10.1262/jrd.2016-158

Yoshikazu Arai, K. Umeyama, Kenta Takeuchi, Natsumi Okazaki, Naomi Hichiwa, S. Yashima, K. Nakano, H. Nagashima, J. Ohgane

{"title":"Establishment of DNA methylation patterns of the Fibrillin1 (FBN1) gene in porcine embryos and tissues","authors":"Yoshikazu Arai, K. Umeyama, Kenta Takeuchi, Natsumi Okazaki, Naomi Hichiwa, S. Yashima, K. Nakano, H. Nagashima, J. Ohgane","doi":"10.1262/jrd.2016-158","DOIUrl":"https://doi.org/10.1262/jrd.2016-158","url":null,"abstract":"DNA methylation in transcriptional regulatory regions is crucial for gene expression. The DNA methylation status of the edges of CpG islands, called CpG island shore, is involved in tissue/cell-type-specific gene expression. Haploinsufficiency diseases are caused by inheritance of one mutated null allele and are classified as autosomal dominant. However, in the same pedigree, phenotypic variances are observed despite the inheritance of the identical mutated null allele, including Fibrillin1 (FBN1), which is responsible for development of the haploinsufficient Marfan disease. In this study, we examined the relationship between gene expression and DNA methylation patterns of the FBN1 CpG island shore focusing on transcriptionally active hypomethylated alleles (Hypo-alleles). No difference in the DNA methylation level of FBN1 CpG island shore was observed in porcine fetal fibroblast (PFF) and the liver, whereas FBN1 expression was higher in PFF than in the liver. However, Hypo-allele ratio of the FBN1 CpG island shore in PFF was higher than that in the liver, indicating that Hypo-allele ratio of the FBN1 CpG island shore likely correlated with FBN1 expression level. In addition, oocyte-derived DNA hypermethylation in preimplantation embryos was erased until the blastocyst stage, and re-methylation of the FBN1 CpG island shore was observed with prolonged in vitro culture of blastocysts. These results suggest that the establishment of the DNA methylation pattern within the FBN1 CpG island shore occurs after the blastocyst stage, likely during organogenesis. In conclusion, Hypo-allele ratios of the FBN1 CpG island shore correlated with FBN1 expression levels in porcine tissues.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116262458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase 利用高斯荧光素酶检测l - β t2细胞激素分泌

The Journal of Reproduction and Development Pub Date : 2017-01-15 DOI: 10.1262/jrd.2016-174

K. Satou, Y. Mochimaru, T. Nakakura, T. Kusada, Jun Negishi, Shiori Musha, N. Yoshimura, Y. Kato, H. Tomura

{"title":"Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase","authors":"K. Satou, Y. Mochimaru, T. Nakakura, T. Kusada, Jun Negishi, Shiori Musha, N. Yoshimura, Y. Kato, H. Tomura","doi":"10.1262/jrd.2016-174","DOIUrl":"https://doi.org/10.1262/jrd.2016-174","url":null,"abstract":"Reproduction is regulated by gonadotropins secreted from gonadotrophs. The production and secretion of gonadotropins are mainly regulated by gonadotropin-releasing hormone (GnRH). Agonists or antagonists that influence GnRH action on gonadotrophs are important to regulate reproduction; however, these factors have not been fully characterized due to the lack of simple and easy-to-use techniques to detect gonadotropin secretion from gonadotropin-producing cells. In the present study, we found that Gaussia luciferase (Gluc), which was expressed in LβT2 cells, can be secreted like a luteinizing-hormone (LH) upon stimulation with GnRH. The Gluc secreted into the medium was easily monitored as luminescence signals. The detection range of the GnRH-induced Gluc activity was comparable to that of the enzyme-linked immunosorbent assay for LH. In addition, when the Gluc was expressed in AtT20 cells, which produce adrenocorticotropic hormone (ACTH), the Gluc activity in the medium increased in parallel with the ACTH secretion upon stimulation with corticotropin-releasing hormone. Thus, the Gluc assay in the present study can be easily used for high-throughput screening of factors that influence LH or ACTH secretion from LβT2 or AtT20 cells, respectively.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128524589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Superovulatory responses in cynomolgus monkeys (Macaca fascicularis) depend on the interaction between donor status and superovulation method used 食蟹猴(Macaca fascularis)的超排卵反应取决于供体状态和使用的超排卵方法之间的相互作用

The Journal of Reproduction and Development Pub Date : 2017-01-09 DOI: 10.1262/jrd.2016-074

Ji-Su Kim, Seung-Bin Yoon, K. Jeong, Bo-Woong Sim, Seon-A Choi, Sangil Lee, Y. Jin, B. Song, Sang-Rae Lee, Sun-Uk Kim, K. Chang

{"title":"Superovulatory responses in cynomolgus monkeys (Macaca fascicularis) depend on the interaction between donor status and superovulation method used","authors":"Ji-Su Kim, Seung-Bin Yoon, K. Jeong, Bo-Woong Sim, Seon-A Choi, Sangil Lee, Y. Jin, B. Song, Sang-Rae Lee, Sun-Uk Kim, K. Chang","doi":"10.1262/jrd.2016-074","DOIUrl":"https://doi.org/10.1262/jrd.2016-074","url":null,"abstract":"The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3 ×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases in ovary size and the mean number of MII oocytes retrieved in age- and body-weight-dependent manner; in contrast, both the parameters were similar in Method B [recombinant gonadotrophin (60 IU, twice daily, 6 days), recombinant gonadotropin and recombinant human luteinizing hormone (rhLH) (60 IU, twice daily, 3 days), and hCG]. Importantly, Method A showed maximal MII oocyte recovery rate in > 60-month-old or 4.5–5.0-kg female monkeys, whereas Method B was equally effective regardless of the donor age and body weight. These results indicate that superovulatory responses depend on the interaction between oocyte donor status and the superovulation method used in cynomolgus monkeys.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117186029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Involvement of calcium channels and intracellular calcium in bull sperm thermotaxis 牛精子热致性中钙通道和细胞内钙的参与

The Journal of Reproduction and Development Pub Date : 2016-12-31 DOI: 10.1262/jrd.2016-107

Md Anisuzzaman Mondal, Y. Takagi, S. Baba, K. Hamano

{"title":"Involvement of calcium channels and intracellular calcium in bull sperm thermotaxis","authors":"Md Anisuzzaman Mondal, Y. Takagi, S. Baba, K. Hamano","doi":"10.1262/jrd.2016-107","DOIUrl":"https://doi.org/10.1262/jrd.2016-107","url":null,"abstract":"Thermotaxis that sperm migrate to higher temperature area has been confirmed in rabbit and human. In this study, we examined the migration ability of bull sperm in a temperature gradient to confirm thermotaxis and elucidate the involvement of calcium in such thermotaxis, as well as the relation between sperm capacitation and bull fertility. Thermotaxis was evaluated in a temperature gradient of 34–42ºC using a cross-type column 22-mm long, 40-mm wide, and 100-μm deep. Significantly more sperm migrated to the high-temperature area of 39ºC in a 2ºC temperature gradient, and to 40ºC in a 1ºC temperature gradient. In calcium-free, BAPTA containing medium, and EGTA containing medium, the migrated sperm ratio in the two temperature areas was almost the same. In media containing lanthanum, ruthenium red, and 2APB, we could not confirm thermotaxis. Pre- and post-capacitated sperm migrated to the high-temperature area, expressing thermotaxis. The sperm from high-fertility bulls showed clear thermotaxis. Based on these results, thermotaxis of bull sperm was confirmed and the involvement of both calcium channels and intracellular stored calcium in thermotaxis was suggested. Although the sample size of bulls was quite small, the difference in thermotaxis may have been associated with bull fertility. Sperm thermotaxis evaluation has potential as a predictor of bull fertility.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115788531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares 母马妊娠早中期子宫脂载素2及其受体的表达

The Journal of Reproduction and Development Pub Date : 2016-12-16 DOI: 10.1262/jrd.2016-096

S. Haneda, K. Nagaoka, Y. Nambo, Masato Kikuchi, Y. Nakano, Junyou Li, M. Matsui, Y. Miyake, K. Imakawa

{"title":"Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares","authors":"S. Haneda, K. Nagaoka, Y. Nambo, Masato Kikuchi, Y. Nakano, Junyou Li, M. Matsui, Y. Miyake, K. Imakawa","doi":"10.1262/jrd.2016-096","DOIUrl":"https://doi.org/10.1262/jrd.2016-096","url":null,"abstract":"From previous cDNA subtraction studies analyzing gene expression in equine endometrium, high lipocalin 2 (LCN2) mRNA expression was found in the gravid endometrium. In the uterus, LCN2 may transport hydrophobic molecules and siderophores with iron, or may form a complex with another protein, however, the expression of uterine LCN2 beyond day 20 of equine pregnancy and its receptor has not been characterized. To study the expression and potential roles of uterine LCN2 from pre-implantation to mid-gestation period, stage-specific endometrial samples were obtained from day 13 (day 0 = ovulation) cyclic and days 13, 19, 25, and 60 to 131 pregnant mares. Expression of LCN2 mRNA increased in day 19 gravid endometrium and was abundant from day 60 onward. The expression of LCN2 mRNA was localized to the glandular epithelium. LCN2 protein was detected in day 25 gravid endometrium and luminal fluid, and the protein was localized to the glandular epithelium and luminal cavity, whereas LCN2 receptor expression was found in luminal and glandular epithelium and trophectoderm throughout the experimental period. The presence of matrix metalloproteinase-9 (MMP9) was also examined because MMP9 is known to form a complex with LCN2. Although MMP9 and LCN2 were both found in luminal fluid from day 25 pregnant uterus, the complex of these proteins was not detected. Localization of the receptor in the trophectoderm suggests that endometrial LCN2 could play a role in carrying small substances from the mother to fetus in the equine species.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132519386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5