The Journal of Reproduction and Development最新文献_第4页

Human chorionic gonadotropin (hCG)-induced ovulation occurs later but with equal occurrence in lactating dairy cows: comparing hCG and gonadotropin-releasing hormone protocols 人绒毛膜促性腺激素(hCG)诱导的排卵发生较晚，但在泌乳奶牛中发生率相同:比较hCG和促性腺激素释放激素方案

The Journal of Reproduction and Development Pub Date : 2019-10-17 DOI: 10.1262/jrd.2019-037

Tsung-Ching Liu, Chia-Tang Ho, Kuang-Po Li, Chao-Chin Chang, Jacky Peng-Wen Chan

{"title":"Human chorionic gonadotropin (hCG)-induced ovulation occurs later but with equal occurrence in lactating dairy cows: comparing hCG and gonadotropin-releasing hormone protocols","authors":"Tsung-Ching Liu, Chia-Tang Ho, Kuang-Po Li, Chao-Chin Chang, Jacky Peng-Wen Chan","doi":"10.1262/jrd.2019-037","DOIUrl":"https://doi.org/10.1262/jrd.2019-037","url":null,"abstract":"This study assessed the effects of two hormones, human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH), on ovulatory responses during different diestrous stages in lactating dairy cows. Estrous cycles of 21 cows were synchronized and were enrolled in stage 1 of the experiment. The cows were treated with a prostaglandin (PG) F2α analog either 9 to 10 days [mid-diestrus (MD) group] or 5.5 to 6.5 days [early-diestrus (ED) group] after synchronized ovulation (day 0 = first PGF2α administration). On day 2, the cows were administrated 250 μg GnRH or 3000 IU hCG. Ovulation was determined every 2 h from 24 to 36 h after GnRH or hCG administration, and then every 4 h up to 72 h until ovulation. Cows in stage 2 were administered these treatments in the reverse order. The results indicated that average ovulation times in cows treated with GnRH in the MD group (GnRH-MD group) and cows treated with GnRH in the ED group (GnRH-ED group) were 30.0 ± 1.0 h and 28.8 ± 0.4 h, respectively. However, ovulation times for cows treated with hCG in the MD group (hCG-MD group) and cows treated with hCG in the ED group (hCG-ED group) were 35.8 ± 4.6 h and 32.8 ± 2.2 h, respectively, and ovulation occurred significantly later in the hCG-treated groups than in the GnRH-treated groups. In summary, we found that hCG-induced ovulation occurred later than GnRH-induced ovulation regardless of different diestrous peroids; however, the two treatments did not differ in terms of percentage of ovulation.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122857163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Xanthan gum and Locust bean gum gel supports in vitro development of porcine oocytes derived from early antral follicles 黄原胶和刺槐豆胶凝胶支持猪早期窦卵泡卵母细胞的体外发育

The Journal of Reproduction and Development Pub Date : 2019-10-06 DOI: 10.1262/jrd.2019-074

Y. Munakata, Ayaka Sugimoto, K. Shirasuna, T. Kuwayama, H. Iwata

引用次数: 3

Prepartum change in ventral tail base surface temperature in beef cattle: comparison with vaginal temperature and behavior indices, and effect of ambient temperature 肉牛腹尾基表面温度的预备变化:与阴道温度和行为指标的比较及环境温度的影响

The Journal of Reproduction and Development Pub Date : 2019-10-06 DOI: 10.1262/jrd.2019-087

M. Miwa, S. Matsuyama, Sho Nakamura, Kohei Noda, M. Sakatani

{"title":"Prepartum change in ventral tail base surface temperature in beef cattle: comparison with vaginal temperature and behavior indices, and effect of ambient temperature","authors":"M. Miwa, S. Matsuyama, Sho Nakamura, Kohei Noda, M. Sakatani","doi":"10.1262/jrd.2019-087","DOIUrl":"https://doi.org/10.1262/jrd.2019-087","url":null,"abstract":"Prediction of parturition is essential for sustainable production in beef and dairy cattle, yet the present methods are limited by their high invasiveness and low utility. Here we compared prepartum changes in ventral tail base surface temperature (ST) with changes in vaginal temperature (VT) and behavioral indices. We analyzed 22 parturitions from 22 beef cows. Changes in daily values of ST, VT, and behavioral indices over the 7 days before parturition were investigated. Hourly values were calculated as the actual values minus the mean values for the same hour over a 3-day period, and the changes in hourly values over the 48 h before parturition were investigated. To test the effect of ambient temperature, tested cows were assigned to two season-groups based on the ambient temperature to which they were exposed (warm: n = 13; cool: n = 9), and the daily and hourly values of the indices were compared between seasons. A decrease in ST occurred approximately 30 h before parturition, which was similar to the time of the decrease in VT and earlier than the increase of behavioral indices. In addition, a unique fluctuation of ST observed in the last few hours before parturition indicates that ST could provide a sign for parturition not only in the long-term like VT, but also in the short-term like behavioral indices. Although ST was more sensitive to ambient temperature than VT or the behavioral indices, the day of parturition could be predicted from ST in both the warm and cool seasons.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126966328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Functional consequences of mitochondrial mismatch in reconstituted embryos and offspring 重组胚胎和后代线粒体失配的功能后果

The Journal of Reproduction and Development Pub Date : 2019-08-29 DOI: 10.1262/jrd.2019-089

K. Takeda

引用次数: 11

Exploring disease-specific methylated CpGs in human male genital abnormalities by using methylated-site display-amplified fragment length polymorphism (MSD-AFLP) 利用甲基化位点显示扩增片段长度多态性(MSD-AFLP)研究人类男性生殖器异常中疾病特异性甲基化CpGs

The Journal of Reproduction and Development Pub Date : 2019-08-29 DOI: 10.1262/jrd.2019-069

Toshiki Aiba, Toshiyuki Saito, A. Hayashi, Shinji Sato, H. Yunokawa, M. Fukami, Y. Hayashi, K. Mizuno, Yuichi Sato, Y. Kojima, S. Ohsako

{"title":"Exploring disease-specific methylated CpGs in human male genital abnormalities by using methylated-site display-amplified fragment length polymorphism (MSD-AFLP)","authors":"Toshiki Aiba, Toshiyuki Saito, A. Hayashi, Shinji Sato, H. Yunokawa, M. Fukami, Y. Hayashi, K. Mizuno, Yuichi Sato, Y. Kojima, S. Ohsako","doi":"10.1262/jrd.2019-069","DOIUrl":"https://doi.org/10.1262/jrd.2019-069","url":null,"abstract":"The incidence of male reproductive system disorders, especially hypospadias, has been increasing in developed countries since the latter half of the 20th century. Endocrine-disrupting chemicals from the environment are considered to be involved in hypospadias onset through epigenetic alterations. This pilot study aimed to explore disease-specific methylated CpGs in human patient samples using the methylated-site display-amplified fragment length polymorphism (MSD-AFLP) technique developed by our research group [1]. We compared clinical samples from hypospadias and phimosis patients. Foreskin and blood samples were collected from one- to two-year-old patients with hypospadias (N = 3) and phimosis (N = 3) during surgical treatment. MSD-AFLP analysis showed significantly decreased CpG-methylation levels of genes such as MYH11 and increased CpG-methylation levels of genes such as PLA2G15 in hypospadias patients. Hierarchical clustering analysis showed that genes with significantly altered CpG levels were more markedly altered in DNA from blood than from foreskin. Because of the small number of samples, further investigation is necessary to elucidate the association between variations in CpG levels in foreskin and blood DNA and male genital abnormalities. However, our MSD-AFLP method appears to be a useful tool for exploring disease-specific methylated-CpGs in human epidemiological studies.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127979360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Early production of offspring by in vitro fertilization using first-wave spermatozoa from prepubertal male mice 利用青春期前雄性小鼠的第一波精子进行体外受精的早期后代生产

The Journal of Reproduction and Development Pub Date : 2019-08-26 DOI: 10.1262/jrd.2019-042

K. Mochida, A. Hasegawa, N. Ogonuki, K. Inoue, A. Ogura

{"title":"Early production of offspring by in vitro fertilization using first-wave spermatozoa from prepubertal male mice","authors":"K. Mochida, A. Hasegawa, N. Ogonuki, K. Inoue, A. Ogura","doi":"10.1262/jrd.2019-042","DOIUrl":"https://doi.org/10.1262/jrd.2019-042","url":null,"abstract":"Mature male mice (aged 10–12 weeks or older) are conventionally used for in vitro fertilization (IVF) in order to achieve high fertilization rates (e.g., > 70%). Here, we sought to determine the earliest age at which male mice (C57BL/6J strain) can be used efficiently for producing offspring via IVF. Because we noted that the addition of reduced glutathione (GSH) to the IVF medium significantly increased the fertilizing ability of spermatozoa from prepubertal males, we used this IVF protocol for all experiments. Spermatozoa first reached the caudal region of the epididymides at day 35; however, they were unable to fertilize oocytes. Caudal epididymal spermatozoa first became competent for oocyte fertilization at day 37, albeit at a low rate (2.9%). A high fertilization rate (72.0%) was obtained at day 40, and 52.4% of the embryos thus obtained developed into offspring after embryo transfer. Moreover, we found that corpus epididymal spermatozoa in prepubertal mice could fertilize oocytes; however, the fertilization rates were always < 50%, regardless of the age of the males. Caput epididymal spermatozoa failed to fertilize oocytes irrespective of the age of the males. Therefore, we propose that caudal epididymal spermatozoa from male mice aged 40 days can be efficiently used for IVF, to obtain offspring in the shortest attainable time. This protocol will reduce the turnover time required for the generation of mice by ~1 month compared with that of the conventional IVF protocol.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125489291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming 解剖生发囊核膜和可溶性物质在体细胞重构和重编程过程中的作用

The Journal of Reproduction and Development Pub Date : 2019-08-18 DOI: 10.1262/jrd.2019-017

H. Fulka, A. Ogura, P. Loi, J. Fulka

{"title":"Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming","authors":"H. Fulka, A. Ogura, P. Loi, J. Fulka","doi":"10.1262/jrd.2019-017","DOIUrl":"https://doi.org/10.1262/jrd.2019-017","url":null,"abstract":"Differentiated nuclei can be reprogrammed/remodelled to totipotency after their transfer to enucleated metaphase II (MII) oocytes. The process of reprogramming/remodelling is, however, only partially characterized. It has been shown that the oocyte nucleus (germinal vesicle – GV) components are essential for a successful remodelling of the transferred nucleus by providing the materials for pseudo-nucleus formation. However, the nucleus is a complex structure and exactly what nuclear components are required for a successful nucleus remodelling and reprogramming is unknown. Till date, the only nuclear sub-structure experimentally demonstrated to be essential is the oocyte nucleolus (nucleolus-like body, NLB). In this study, we investigated what other GV components might be necessary for the formation of normal-sized pseudo-pronuclei (PNs). Our results showed that the removal of the GV nuclear envelope with attached chromatin and chromatin-bound factors does not substantially influence the size of the remodelled nuclei in reconstructed cells and that their nuclear envelopes seem to have normal parameters. Rather than the insoluble nuclear lamina, the GV content, which is dissolved in the cytoplasm with the onset of oocyte maturation, influences the characteristics and size of transferred nuclei.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131588515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The efficacy of a newly developed neurokinin 3 receptor agonist B21-750 on luteinizing hormone secretion in cycling goats 新研制的神经激肽3受体激动剂B21-750对循环山羊黄体生成素分泌的影响

The Journal of Reproduction and Development Pub Date : 2019-08-12 DOI: 10.1262/jrd.2019-038

L. P. Rahayu, N. Endo, Shinji Kuwai, S. Oishi, Tomomi Tanaka

引用次数: 0

Role of endoplasmic reticulum stress in lipopolysaccharide-inhibited mouse granulosa cell estradiol production 内质网应激在脂多糖抑制小鼠颗粒细胞雌二醇产生中的作用

The Journal of Reproduction and Development Pub Date : 2019-08-11 DOI: 10.1262/jrd.2019-052

L. Lei, Junbang Ge, Hui Zhao, Xiangguo Wang, Lei Yang

{"title":"Role of endoplasmic reticulum stress in lipopolysaccharide-inhibited mouse granulosa cell estradiol production","authors":"L. Lei, Junbang Ge, Hui Zhao, Xiangguo Wang, Lei Yang","doi":"10.1262/jrd.2019-052","DOIUrl":"https://doi.org/10.1262/jrd.2019-052","url":null,"abstract":"The decrease in the level of estradiol (E2) in granulosa cells caused by lipopolysaccharide (LPS) is one of the major causes of infertility underlying postpartum uterine infections; the precise molecular mechanism of which remains elusive. This study investigated the role of endoplasmic reticulum (ER) stress in LPS-induced E2 decrease in mouse granulosa cells. Our results showed that LPS increased the pro-inflammatory cytokines [(interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α)], activated ER stress marker protein expression [(glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP)], and decreased cytochrome P450 family 19 subfamily A member 1 (Cyp19a1) expression and E2 production. Moreover, inhibition of ER stress by 4-phenylbutyrate (4-PBA) attenuated thapsigargin-(TG, ER stress agonist) or LPS-induced reduction of Cyp19a1 and E2, pro-inflammatory cytokines expression (IL-1β, IL-6, IL-8, and TNF-α), and the expression of CHOP and GRP78. Additionally, inhibition of toll-like receptor 4 (TLR4) by resatorvid (TAK-242) reversed the inhibitory effects of LPS on Cyp19a1 expression and E2 production, activation of GRP78 and CHOP, and expression of IL-1β, IL-6, IL-8, and TNF-α. In summary, our study suggests that ER stress is involved in LPS-inhibited E2 production in mouse granulosa cells.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130335352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2α and Ovsynch-CIDR estrus synchronization treatments CIDR-PGF2α和ovsync - cidr发情同步处理下埃及水牛卵巢卵泡的变化和血流动力学

The Journal of Reproduction and Development Pub Date : 2019-08-10 DOI: 10.1262/jrd.2019-035

H. Samir, M. Kandiel, A. A. El-Maaty, Manila Sediqyar, K. Sasaki, G. Watanabe

{"title":"Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2α and Ovsynch-CIDR estrus synchronization treatments","authors":"H. Samir, M. Kandiel, A. A. El-Maaty, Manila Sediqyar, K. Sasaki, G. Watanabe","doi":"10.1262/jrd.2019-035","DOIUrl":"https://doi.org/10.1262/jrd.2019-035","url":null,"abstract":"This study aimed at investigating the efficacy of two protocols of estrous synchronization on follicular changes and hemodynamics. Pluriparous Egyptian buffaloes (n = 36) were synchronized either with controlled internal drug release (CIDR)-PGF2α (7-days CIDR insert with PGF2α injected on the 6th day; n = 18) or Ovsynch-CIDR (Ovsynch protocol concurrent with 7-days CIDR insert; n = 18). Blood sampling and ovarian ultrasound examinations (Grayscale, color and power Doppler modes) were conducted on the Day of CIDR removal, estrus, and luteal phase. Mean follicle diameter (MFD), first (1st-LF) and second (2nd-LF) largest follicle diameters, and E2 levels significantly increased in the CIDR-PGF2α group at CIDR withdrawal. Ovsynch-CIDR markedly fortified higher follicle population, MFD, and 1st-LF diameter at estrus and corpus luteum (CL) volume at the luteal phase in concomitant with increases (P < 0.05) in E2 (at estrus) and P4 (at luteal phase). At CIDR removal, the blue pixels in the dominant follicle (DF) were higher (1.5 times; P = 0.054) in the Ovsynch-CIDR than in the CIDR-PGF2α. At estrus, total blood flow (TBF) and power Doppler pixels (PDP) to DF(s) were noticeably higher (seven and 1.6 times; respectively) in the Ovsynch-CIDR than in CIDR-PGF2α (5906 ± 237 vs. 830 ± 60 pixels, P < 0.01 and 5479 ± 322 vs. 3377 ± 19 pixels, P < 0.05; respectively). At the luteal phase, TBF and PDP to the CL increased in the Ovsynch-CIDR group than in the CIDR-PGF2α group (11060 ± 965 vs. 7963 ± 480 pixels, 1.4 times, P = 0.05 and 18900 ± 1350 vs. 13220 ± 568 pixels, 1.1 times, P = 0.005; respectively). In conclusion, based on the improvement in synchronized follicular activity and hemodynamics, the Ovsynch-CIDR regimen is persuaded in Egyptian buffaloes.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133624167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14