The Journal of Reproduction and Development最新文献_第3页

Expression of C–C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition C-C基序趋化因子及其受体在牛自然分娩和诱导分娩中的表达

The Journal of Reproduction and Development Pub Date : 2019-11-22 DOI: 10.1262/jrd.2019-113

Hiroki Hirayama, R. Sakumoto, K. Koyama, Taichi Yasuhara, Taito Hasegawa, Ryosuke Inaba, T. Fujii, A. Naito, S. Moriyasu, S. Kageyama

{"title":"Expression of C–C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition","authors":"Hiroki Hirayama, R. Sakumoto, K. Koyama, Taichi Yasuhara, Taito Hasegawa, Ryosuke Inaba, T. Fujii, A. Naito, S. Moriyasu, S. Kageyama","doi":"10.1262/jrd.2019-113","DOIUrl":"https://doi.org/10.1262/jrd.2019-113","url":null,"abstract":"In bovine placentomes, the inflammatory response is considered important for the detachment of the fetal membrane from the caruncle after parturition. Glucocorticoids, a trigger of the onset of parturition, facilitate functional maturation of placentomes via prostaglandin (PG) and estrogen production in cattle. This study investigated how exogeneous glucocorticoids, which exert immunosuppressive effects, affect placental inflammation at parturition. Placentomes were collected immediately after spontaneous or induced parturition. Parturition was conventionally induced using PGF2α or dexamethasone or with a combination of triamcinolone acetonide and high-dose betamethasone (TABET treatment). Polymerase chain reaction (PCR) array analysis indicated that 9/13 C–C motif chemokine ligands (CCLs) were upregulated > two-fold in spontaneous parturition, with CCL2 and CCL8 being highly expressed. The expressions of CCL2, CCL8, C–C motif chemokine receptor 1 (CCR1), and CCR5 in caruncles were significantly higher in spontaneous parturition than in induced parturition. Although the clinical dose of dexamethasone did not influence the expression of these CCLs and CCRs, TABET treatment increased CCR1 expression. CCL8, CCR1, CCR2, and CCR5 were localized in the caruncular epithelial cells. CCR2 was also localized in the epithelial cells of the cotyledonary villi. This study is the first report to reveal the disruption in CCL and CCR expression in bovine placentomes at induced parturition. Enhanced glucocorticoid exposure for the induction of parturition may upregulate CCR1 expression in placentomes, but the treatment does not adequately promote CCL expression. Additionally, immunohistochemistry suggested that the CCL–CCR system is involved in the functional regulation of maternal and fetal epithelial cells in placentomes at parturition.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129176705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Improved embryo survival following follicular drainage of subordinate follicles for twin pregnancy prevention in bi-ovular dairy cows 辅助卵泡引流预防双卵奶牛双胎妊娠提高胚胎存活率

The Journal of Reproduction and Development Pub Date : 2019-11-16 DOI: 10.1262/jrd.2019-065

I. Garcia-Ispierto, F. López-Gatius

引用次数: 6

Melatonin protects against Epirubicin-induced ovarian damage 褪黑素可以防止表柔比星引起的卵巢损伤

The Journal of Reproduction and Development Pub Date : 2019-11-15 DOI: 10.1262/jrd.2019-085

Naiqiang Wang, Hua Li, Yunqing Zhu, Na Li, Zi-jiang Chen, Cong Zhang

{"title":"Melatonin protects against Epirubicin-induced ovarian damage","authors":"Naiqiang Wang, Hua Li, Yunqing Zhu, Na Li, Zi-jiang Chen, Cong Zhang","doi":"10.1262/jrd.2019-085","DOIUrl":"https://doi.org/10.1262/jrd.2019-085","url":null,"abstract":"One major side effect of chemotherapy that young women with cancer suffer from is ovarian damage. Therefore, it is necessary to study the pathogenesis of chemotherapeutic drugs in order to develop pharmaceutical agents to preserve fertility. Epirubicin is one of the commonly used chemotherapy drugs for breast cancer patients. This research explored the side effects of epirubicin in mice. We found that epirubicin significantly reduced the body weight, the weight of the ovaries and uteri, and the pups’ number, while melatonin, which is extremely resistant to oxidation, significantly reduced these damages. Moreover, co-treatment with melatonin prevented epirubicin-induced decrease in E2 and progesterone, and the loss of follicles. Mechanism study showed that melatonin significantly reduced the levels of proapoptotic genes p53, Caspase3, and Caspase9 while it upregulated antiapoptotic factors Bcl-2 and Bcl2l1, and antioxidant genes superoxide dismutase 1 and catalase compared with the epirubicin group. In addition, melatonin markedly reduced reactive oxygen species (ROS) and the transcription of Caspase12 and Chop, which is vital in endoplasmic reticulum stress (ERS)-mediated apoptosis. These results indicate melatonin protects against epirubicin-induced ovarian damage by reducing ROS-induced ERS. Therefore, melatonin has a therapeutic potential for the protection of ovarian function and preservation of fertility during chemotherapy.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131208102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Effects of 5-hydroxytryptamine on spermatozoal hyperactivation and in vitro fertilization in mice 5-羟色胺对小鼠精子过度活化及体外受精的影响

The Journal of Reproduction and Development Pub Date : 2019-11-06 DOI: 10.1262/jrd.2019-082

Yukiko Sugiyama, M. Fujinoki, H. Shibahara

{"title":"Effects of 5-hydroxytryptamine on spermatozoal hyperactivation and in vitro fertilization in mice","authors":"Yukiko Sugiyama, M. Fujinoki, H. Shibahara","doi":"10.1262/jrd.2019-082","DOIUrl":"https://doi.org/10.1262/jrd.2019-082","url":null,"abstract":"In this study, we examined the effects of 5-hydroxytryptamine (5-HT) on the motility and hyperactivation of mouse spermatozoa. In addition, we examined whether 5-HT increases the success of in vitro fertilization (IVF) in mice. Interestingly, 5-HT and agonists of the 5-HT2, 5-HT3, 5-HT4, and 5-HT7 receptors significantly increased the percentage of hyperactivated spermatozoa but did not affect the percentage of motile spermatozoa. Moreover, agonists of the 5-HT2, 5-HT3, and 5-HT4 receptors significantly affected the velocities, linearity, straightness, wobbler coefficient, amplitude and/or frequency of spermatozoa. In particular, the improvement of hyperactivation by 5-HT was strongly inhibited by antagonists of the receptors 5-HT4 and 5-HT7 and was completely inhibited by a mixture of the four 5-HT-receptor antagonists. The increase in hyperactivation by the agonists was significantly inhibited by the corresponding 5-HT-receptor antagonist. Moreover, 5-HT significantly increased the percentage of two-cell embryos. The increase in the IVF success rate by 5-HT was significantly inhibited by a 5-HT4-receptor antagonist. These results suggest that 5-HT increased hyperactivation through the 5-HT receptors and increased the success of IVF in mice.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"511 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127603953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles 自噬诱导剂和自噬抑制剂对猪中小卵泡卵母细胞的减数分裂和发育能力有不同的影响

The Journal of Reproduction and Development Pub Date : 2019-11-03 DOI: 10.1262/jrd.2019-112

Chiyuki Kohata-Ono, T. Wakai, H. Funahashi

{"title":"The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles","authors":"Chiyuki Kohata-Ono, T. Wakai, H. Funahashi","doi":"10.1262/jrd.2019-112","DOIUrl":"https://doi.org/10.1262/jrd.2019-112","url":null,"abstract":"This study aimed to examine the effect of rapamycin (autophagy inducer) and 3-methyladenine (3-MA, autophagy inhibitor) on the meiotic and developmental competencies of porcine oocytes derived from medium follicles (MF, 3–6 mm in diameter) and small follicles (SF, 1–2 mm in diameter) during in vitro maturation (IVM) process. The presence of 1 nM but not 10 nM rapamycin significantly increased the maturation rate of MF-derived oocytes (P < 0.05). However, the maturation rate of SF-derived oocytes was not affected by rapamycin at both concentrations (1 nM and 10 nM). The maturation rate of MF-derived oocytes decreased significantly (P < 0.05) in the presence of 0.2 mM but not 2 mM 3-MA than non-supplemented control. In contrast, in SF-derived oocytes, 3-MA at both 0.2 and 2 mM concentrations did not affect the maturation rates. The presence of 1 nM rapamycin significantly increased the blastocyst formation rate of MF-derived mature oocytes following parthenogenetic activation (P < 0.05). However, the blastocyst formation rate of SF-derived mature oocytes was not affected by the presence of rapamycin. The presence of 3-MA significantly reduced the blastocyst formation rate of MF-derived mature oocytes but did not change that of SF-derived oocytes. In conclusion, our study results show differences in activity of the autophagy inducer and inhibitor on the meiotic and developmental competencies of MF- and SF-derived porcine oocytes.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129338419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos 溶酶体组织蛋白酶在牛卵母细胞和着床前胚胎中的动态变化

The Journal of Reproduction and Development Pub Date : 2019-11-03 DOI: 10.1262/jrd.2019-115

Jianye Li, Mana Maeji, A. Balboula, Mansour Aboelenain, T. Fujii, S. Moriyasu, H. Bai, M. Kawahara, Masashi Takahashi

{"title":"Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos","authors":"Jianye Li, Mana Maeji, A. Balboula, Mansour Aboelenain, T. Fujii, S. Moriyasu, H. Bai, M. Kawahara, Masashi Takahashi","doi":"10.1262/jrd.2019-115","DOIUrl":"https://doi.org/10.1262/jrd.2019-115","url":null,"abstract":"Lysosomal cathepsin, in particular cathepsin B (CTSB), plays an important role in implantation, pregnancy, and embryonic development. However, little is known about the mechanism related to the dynamic status of lysosomal cathepsins in bovine oocytes and preimplantation embryos. In the present study, we investigated the dynamics of gene expression, activity, and immunolocalization of CTSB, as well as the activities of lysosome, in bovine oocytes and preimplantation embryos. After gene expression analysis of several cathepsin-related genes, transcript levels of CTSB, CTSD and CTSZ were highest in Metaphase II (MII) oocytes followed by a significant decrease from the 8-cell embryo stage. Activity of CTSB showed a significant increase in 1-cell and morula stage embryos. Lysosomal activity was also significant higher in 1-cell and morula stages, which was consistent with CTSB activities. However, immunolocalization of CTSB did not show the similar pattern of CTSB and lysosomal activities. We also found significantly higher expression levels of CTSB transcript in the trophectoderm (TE) compared to inner cell mass (ICM), whereas activity and immunolocalization of CTSB showed an opposite pattern, i.e. significantly higher in ICM than TE. These patterns were confirmed by the same analysis using separated ICM and TE. Our results suggest that lysosomal CTSB has a pivotal role during embryonic development and differentiation, especially fertilization and the differentiation period.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132351129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Improvement of developmental competence of cloned male pig embryos by short hairpin ribonucleic acid (shRNA) vector-based but not small interfering RNA (siRNA)-mediated RNA interference (RNAi) of Xist expression 基于短发夹核糖核酸(shRNA)载体而非小干扰RNA (siRNA)介导的Xist表达的RNA干扰(RNAi)提高克隆雄性猪胚胎发育能力

The Journal of Reproduction and Development Pub Date : 2019-10-21 DOI: 10.1262/jrd.2019-070

X. Yang, Xiao Wu, Yang Yang, Ting Gu, L. Hong, E. Zheng, Zheng Xu, fang zeng, Junsong Shi, Rong Zhou, Gengyuan Cai, Zhenfang Wu, Zicong Li

{"title":"Improvement of developmental competence of cloned male pig embryos by short hairpin ribonucleic acid (shRNA) vector-based but not small interfering RNA (siRNA)-mediated RNA interference (RNAi) of Xist expression","authors":"X. Yang, Xiao Wu, Yang Yang, Ting Gu, L. Hong, E. Zheng, Zheng Xu, fang zeng, Junsong Shi, Rong Zhou, Gengyuan Cai, Zhenfang Wu, Zicong Li","doi":"10.1262/jrd.2019-070","DOIUrl":"https://doi.org/10.1262/jrd.2019-070","url":null,"abstract":"Xist is an X-linked ribonucleic acid (RNA) gene responsible for the cis induction of X chromosome inactivation (XCI). In cloned mammalian embryos, Xist is ectopically activated at the morula to blastocyst stage on the X chromosome that is supposed to be active, thus resulting in abnormal XCI. Suppression of erroneous Xist expression by injecting small interfering RNA (siRNA) remarkably increased the developmental efficiency of cloned male mouse embryos by approximately 10-fold. However, injection of anti-Xist siRNA resulted in only a slight increase in the developmental ability of injected cloned male pig embryos because the blocking effect of the injected siRNA was not maintained beyond the morula stage, which is 5 days post-activation. To develop a more effective approach for suppressing the ectopic expression of Xist in cloned pig embryos, we compared the silencing effect of short hairpin RNA (shRNA) and siRNA on Xist expression and the effects of these two Xist knockdown methods on the developmental competence of cloned male pig embryos. Results indicated that an shRNA-based RNA interference (RNAi) has a longer blocking effect on Xist expression than an siRNA-mediated RNAi. Injection of anti-Xist shRNA plasmid into two-cell-stage cloned male pig embryos effectively suppressed Xist expression, rescued XCI at the blastocyst stage, and improved the in vitro developmental ability of injected cloned embryos. These positive effects, however, were not observed in cloned male pig embryos injected with anti-Xist siRNA. This study demonstrates that vector-based rather than siRNA-mediated RNAi of Xist expression can be employed to improve pig cloning efficiency.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126325640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Human chorionic gonadotropin (hCG)-induced ovulation occurs later but with equal occurrence in lactating dairy cows: comparing hCG and gonadotropin-releasing hormone protocols 人绒毛膜促性腺激素(hCG)诱导的排卵发生较晚，但在泌乳奶牛中发生率相同:比较hCG和促性腺激素释放激素方案

The Journal of Reproduction and Development Pub Date : 2019-10-17 DOI: 10.1262/jrd.2019-037

Tsung-Ching Liu, Chia-Tang Ho, Kuang-Po Li, Chao-Chin Chang, Jacky Peng-Wen Chan

{"title":"Human chorionic gonadotropin (hCG)-induced ovulation occurs later but with equal occurrence in lactating dairy cows: comparing hCG and gonadotropin-releasing hormone protocols","authors":"Tsung-Ching Liu, Chia-Tang Ho, Kuang-Po Li, Chao-Chin Chang, Jacky Peng-Wen Chan","doi":"10.1262/jrd.2019-037","DOIUrl":"https://doi.org/10.1262/jrd.2019-037","url":null,"abstract":"This study assessed the effects of two hormones, human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH), on ovulatory responses during different diestrous stages in lactating dairy cows. Estrous cycles of 21 cows were synchronized and were enrolled in stage 1 of the experiment. The cows were treated with a prostaglandin (PG) F2α analog either 9 to 10 days [mid-diestrus (MD) group] or 5.5 to 6.5 days [early-diestrus (ED) group] after synchronized ovulation (day 0 = first PGF2α administration). On day 2, the cows were administrated 250 μg GnRH or 3000 IU hCG. Ovulation was determined every 2 h from 24 to 36 h after GnRH or hCG administration, and then every 4 h up to 72 h until ovulation. Cows in stage 2 were administered these treatments in the reverse order. The results indicated that average ovulation times in cows treated with GnRH in the MD group (GnRH-MD group) and cows treated with GnRH in the ED group (GnRH-ED group) were 30.0 ± 1.0 h and 28.8 ± 0.4 h, respectively. However, ovulation times for cows treated with hCG in the MD group (hCG-MD group) and cows treated with hCG in the ED group (hCG-ED group) were 35.8 ± 4.6 h and 32.8 ± 2.2 h, respectively, and ovulation occurred significantly later in the hCG-treated groups than in the GnRH-treated groups. In summary, we found that hCG-induced ovulation occurred later than GnRH-induced ovulation regardless of different diestrous peroids; however, the two treatments did not differ in terms of percentage of ovulation.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122857163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Xanthan gum and Locust bean gum gel supports in vitro development of porcine oocytes derived from early antral follicles 黄原胶和刺槐豆胶凝胶支持猪早期窦卵泡卵母细胞的体外发育

The Journal of Reproduction and Development Pub Date : 2019-10-06 DOI: 10.1262/jrd.2019-074

Y. Munakata, Ayaka Sugimoto, K. Shirasuna, T. Kuwayama, H. Iwata

引用次数: 3

Prepartum change in ventral tail base surface temperature in beef cattle: comparison with vaginal temperature and behavior indices, and effect of ambient temperature 肉牛腹尾基表面温度的预备变化:与阴道温度和行为指标的比较及环境温度的影响

The Journal of Reproduction and Development Pub Date : 2019-10-06 DOI: 10.1262/jrd.2019-087

M. Miwa, S. Matsuyama, Sho Nakamura, Kohei Noda, M. Sakatani

{"title":"Prepartum change in ventral tail base surface temperature in beef cattle: comparison with vaginal temperature and behavior indices, and effect of ambient temperature","authors":"M. Miwa, S. Matsuyama, Sho Nakamura, Kohei Noda, M. Sakatani","doi":"10.1262/jrd.2019-087","DOIUrl":"https://doi.org/10.1262/jrd.2019-087","url":null,"abstract":"Prediction of parturition is essential for sustainable production in beef and dairy cattle, yet the present methods are limited by their high invasiveness and low utility. Here we compared prepartum changes in ventral tail base surface temperature (ST) with changes in vaginal temperature (VT) and behavioral indices. We analyzed 22 parturitions from 22 beef cows. Changes in daily values of ST, VT, and behavioral indices over the 7 days before parturition were investigated. Hourly values were calculated as the actual values minus the mean values for the same hour over a 3-day period, and the changes in hourly values over the 48 h before parturition were investigated. To test the effect of ambient temperature, tested cows were assigned to two season-groups based on the ambient temperature to which they were exposed (warm: n = 13; cool: n = 9), and the daily and hourly values of the indices were compared between seasons. A decrease in ST occurred approximately 30 h before parturition, which was similar to the time of the decrease in VT and earlier than the increase of behavioral indices. In addition, a unique fluctuation of ST observed in the last few hours before parturition indicates that ST could provide a sign for parturition not only in the long-term like VT, but also in the short-term like behavioral indices. Although ST was more sensitive to ambient temperature than VT or the behavioral indices, the day of parturition could be predicted from ST in both the warm and cool seasons.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126966328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11