The Journal of Reproduction and Development最新文献_第10页

Liver condition of Holstein cows affects mitochondrial function and fertilization ability of oocytes 荷斯坦奶牛肝脏状况影响线粒体功能和卵母细胞受精能力

The Journal of Reproduction and Development Pub Date : 2016-01-30 DOI: 10.1262/jrd.2015-143

Hiroshi Tanaka, S. Takeo, T. Abe, A. Kin, K. Shirasuna, T. Kuwayama, H. Iwata

{"title":"Liver condition of Holstein cows affects mitochondrial function and fertilization ability of oocytes","authors":"Hiroshi Tanaka, S. Takeo, T. Abe, A. Kin, K. Shirasuna, T. Kuwayama, H. Iwata","doi":"10.1262/jrd.2015-143","DOIUrl":"https://doi.org/10.1262/jrd.2015-143","url":null,"abstract":"The aim of the present study was to examine the fertilization ability and mitochondrial function of oocytes derived from cows with or without liver damage. Oocytes were collected from the ovaries of cows with damaged livers (DL) and those of cows with healthy livers (HL), subjected to in vitro maturation, and fertilized in vitro. A significantly high abnormal fertilization rate was observed for oocytes from DL cows compared to oocytes from HL cows. The time to dissolve the zona pellucida by protease before fertilization was similar between the two liver conditions, whereas after fertilization treatment this time was shorter for DL cows than for HL cows. The percentage of oocytes with equivalent cortical granule distributions underneath the membrane was greater for in vitro matured oocytes from HL cows, whereas an immature distribution pattern was observed for oocytes from DL cows. In addition, a greater percentage of oocytes derived from HL cows released cortical granules following fertilization compared with oocytes from DL cows. Mitochondrial function determined by ATP content and membrane potential were similar at the germinal vesicle stage, but post-in vitro maturation, the oocytes derived from HL cows showed higher values than DL cows. The mitochondrial DNA copy number in oocytes was similar between the two liver conditions for both the germinal vesicle and post-in vitro maturation oocytes. In conclusion, liver damage induces low fertilization, likely because of incomplete cortical granule distribution and release, and the maturation of oocytes from DL cows contain low-functioning mitochondria compared to their HL counterparts.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"206 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131942658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells 从三原核受精卵中提取的三倍体人胚胎干细胞表现出与二倍体人胚胎干细胞相似的多能性和滋养细胞分化能力

The Journal of Reproduction and Development Pub Date : 2016-01-28 DOI: 10.1262/jrd.2015-113

R. Rungsiwiwut, P. Numchaisrika, V. Ahnonkitpanit, P. Virutamasen, K. Pruksananonda

{"title":"Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells","authors":"R. Rungsiwiwut, P. Numchaisrika, V. Ahnonkitpanit, P. Virutamasen, K. Pruksananonda","doi":"10.1262/jrd.2015-113","DOIUrl":"https://doi.org/10.1262/jrd.2015-113","url":null,"abstract":"Because the diploid human embryonic stem cells (hESCs) can be successfully derived from tripronuclear zygotes thus, they can serve as an alternative source of derivation of normal karyotype hESC lines. The aim of the present study was to compare the pluripotency and trophoblast differentiation ability of hESCs derived from tripronuclear zygotes and diploid hESCs. In the present study, a total of 20 tripronuclear zygotes were cultured; 8 zygotes developed to the blastocyst stage and 1 hESC line was generated. Unlike the previous studies, chromosomal correction of tripronuclear zygotes during derivation of hESCs did not occur. The established line carries 3 sets of chromosomes and showed a numerical aberration. Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both in vitro and in vivo. The expression of X inactive specific transcript (XIST) in mid-passage (passage 42) of undifferentiated triploid hESCs was detected, indicating X chromosome inactivation of the cell line. Moreover, when this cell line was induced to differentiate toward the trophoblast lineage, morphological and functional trophoblast cells were observed, similar to the diploid hESC line.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115136760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer 体外受精和体细胞核移植获得的猪胚胎干细胞的超微结构比较

The Journal of Reproduction and Development Pub Date : 2016-01-28 DOI: 10.1262/jrd.2015-124

Hyunju Yoo, Eunhye Kim, Seon-Ung Hwang, J. Yoon, Y. Jeon, Kyu-Mi Park, Kyu-Jun Kim, Minghui Jin, Chang-Kyu Lee, Eunsong Lee, Hyunggee Kim, Gonhyung Kim, S. Hyun

{"title":"Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer","authors":"Hyunju Yoo, Eunhye Kim, Seon-Ung Hwang, J. Yoon, Y. Jeon, Kyu-Mi Park, Kyu-Jun Kim, Minghui Jin, Chang-Kyu Lee, Eunsong Lee, Hyunggee Kim, Gonhyung Kim, S. Hyun","doi":"10.1262/jrd.2015-124","DOIUrl":"https://doi.org/10.1262/jrd.2015-124","url":null,"abstract":"The ultrastructure of porcine putative embryonic stem cells and porcine fetal fibroblasts (PFFs) was analyzed by transmission electron microscopy. The aim of this study was to compare the features of organelles in in vitro fertilization (IVF) derived porcine embryonic stem cells (IVF-pESCs) and somatic cell nuclear transfer (SCNT) derived pESCs (SCNT-pESCs). Also, the features of organelles in high-passage IVF-pESCs were compared with those in low-passage cells. The ultrastructure of PFFs showed rare microvilli on the cell surfaces, polygonal or irregular nuclei with one to two reticular-shaped nucleoli and euchromatin, low cytoplasm-to-nucleus ratios, rare ribosomes, rare rough endoplasmic reticulum, elongated mitochondria, rich lysosomes and rich phagocytic vacuoles. IVF-pESCs showed rare microvilli on the cell surfaces, round or irregular nuclei with one to two reticular-shaped nucleoli and euchromatin, low cytoplasm-to-nucleus ratios, rich ribosomes, long stacks of rough endoplasmic reticulum, elongated mitochondria, rare lysosomes and rare autophagic vacuoles. By contrast, SCNT-pESCs showed rich microvilli with various lengths and frequencies on the cell surfaces, polygonal nuclei with one reticular shaped nucleoli and heterochromatin, high cytoplasm-to-nucleus ratios, rare ribosomes, rare rough endoplasmic reticulum, round mitochondria, rich lysosomes and rich phagocytic vacuoles with clear intercellular junctions. Furthermore, high-passage IVF-pESCs showed irregularly shaped colonies, pyknosis and numerous lysosomes associated with autophagic vacuoles showing signs of apoptosis. In conclusion, this study confirms that the ultrastructural characteristics of pESCs differ depending on their origin. These ultrastructural characteristics might be useful in biomedical research using pESCs, leading to new insights regarding regenerative medicine and tissue repair.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114692299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos 延时监测显示，玻璃化增加了小鼠胚胎在孵化前阶段收缩的频率

The Journal of Reproduction and Development Pub Date : 2016-01-25 DOI: 10.1262/jrd.2015-150

Yuki Shimoda, J. Kumagai, Mibuki Anzai, K. Kabashima, K. Togashi, Yasuko Miura, H. Shirasawa, W. Sato, Y. Kumazawa, Y. Terada

{"title":"Time-lapse monitoring reveals that vitrification increases the frequency of contraction during the pre-hatching stage in mouse embryos","authors":"Yuki Shimoda, J. Kumagai, Mibuki Anzai, K. Kabashima, K. Togashi, Yasuko Miura, H. Shirasawa, W. Sato, Y. Kumazawa, Y. Terada","doi":"10.1262/jrd.2015-150","DOIUrl":"https://doi.org/10.1262/jrd.2015-150","url":null,"abstract":"Contraction during the blastocyst stage is observed during embryonic development of various mammals, including humans, but the physiological role of this process is not well understood. Using time-lapse monitoring (TLM), we studied the influence of vitrification and contractions on embryonic development in mice. Mouse embryos were cultured at the 2-cell stage. At the 8-cell stage, embryos were randomly divided into a fresh group (FG) and vitrified group (VG) and observed for up to 144 h. Strong contractions (i.e., contractions causing a decrease in volume of more than 20% and expansion of the perivitelline space) occurred significantly more often in unhatched embryos than hatching embryos in both groups. Regarding hatching embryos, contractions in the pre-hatching stage were significantly more frequent in the VG than the FG. Furthermore, mRNA expression levels of genes related to contractions were determined at three time points, the 8-cell stage, early blastocyst stage, and 20 h after blastocoel formation, with quantitative reverse transcription-polymerase chain reaction. There was no significant difference in Hspa1a expression between the FG and VG, but Hspa1a overexpression was observed just after thawing and tended to decrease gradually thereafter in some blastocysts. Furthermore, in the VG, Atp1a1 tended to show higher expression in the strong contraction group than in the weak contraction group. Overall, vitrification is an excellent method for cryopreservation but could increase contractions in the pre-hatching stage and may increase energy demands of the embryo. Observation of contraction by TLM may improve the evaluation of embryo quality.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121271673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: A physiological implication in bovine oviduct immunity 内皮素-1下调中性粒细胞体外精子吞噬:牛输卵管免疫的生理意义

The Journal of Reproduction and Development Pub Date : 2016-01-18 DOI: 10.1262/jrd.2015-112

M. Marey, M. Yousef, Jing-hui Liu, Kazuhiro Morita, M. Sasaki, H. Hayakawa, Takashi Shimizu, Ibrahim I. Elshahawy, A. Miyamoto

{"title":"Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: A physiological implication in bovine oviduct immunity","authors":"M. Marey, M. Yousef, Jing-hui Liu, Kazuhiro Morita, M. Sasaki, H. Hayakawa, Takashi Shimizu, Ibrahim I. Elshahawy, A. Miyamoto","doi":"10.1262/jrd.2015-112","DOIUrl":"https://doi.org/10.1262/jrd.2015-112","url":null,"abstract":"The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as determined by EIA. Phagocytosis and superoxide production were assayed by co-incubation of sperm pretreated to induce capacitation with PMNs exposed to EDN-1 (0, 10–11, 10–10, 10–9, and 10–8 M) for 2 h. EDN-1 suppressed dose dependently (10–11 to 10–8 M) the phagocytic activity for sperm and superoxide production of PMNs in response to capacitated sperm. Moreover, this suppression was eliminated by an ETB receptor antagonist (BQ-788). EDN-1 suppressed mRNA expression of EDN-1 and ETB but not ETA receptors in PMNs, suggesting the ETB receptor-mediated pathway. Scanning electron microscopic observation revealed that incubation of PMNs with EDN-1 (10–9 M) completely suppressed the formation of DNA-based neutrophil extracellular traps for sperm entanglement. The results provide evidence indicating that EDN-1 may be involved in the protection of sperm from phagocytosis by PMNs in the bovine oviduct, supporting sperm survival until fertilization.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114788472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Herp depletion arrests the S phase of the cell cycle and increases estradiol synthesis in mouse granulosa cells 在小鼠颗粒细胞中，疱疹耗竭抑制细胞周期的S期并增加雌二醇的合成

The Journal of Reproduction and Development Pub Date : 2016-01-15 DOI: 10.1262/jrd.2015-120

Fenglei Chen, Nan Wang, Diqi Yang, Xin Wen, T. N. Mahmoud, Dong Zhou, Keqiong Tang, P. Lin, Aihua Wang, Yaping Jin

{"title":"Herp depletion arrests the S phase of the cell cycle and increases estradiol synthesis in mouse granulosa cells","authors":"Fenglei Chen, Nan Wang, Diqi Yang, Xin Wen, T. N. Mahmoud, Dong Zhou, Keqiong Tang, P. Lin, Aihua Wang, Yaping Jin","doi":"10.1262/jrd.2015-120","DOIUrl":"https://doi.org/10.1262/jrd.2015-120","url":null,"abstract":"The endoplasmic reticulum (ER) stress response has been implicated in the development, atresia and luteinization of ovarian follicles. However, there have been few reports concerning the role of Herp, an ER stress-induced protein, in follicular development. The present study aims to detect the distribution and cyclic variations of Herp during the estrous cycle and to reveal the roles of Herp in regulating the cell cycle, apoptosis and steroid hormone biosynthesis in mouse granulosa cells. In this study, immunohistochemistry staining showed that Herp expression was primarily in the granulosa cells and oocytes. Furthermore, we constructed recombinant lentiviral vectors for Herp short hairpin interfering RNA (shRNA) expression; immunofluorescence staining, real-time quantitative PCR (RT-qPCR) and western blot analysis revealed that Herp was successfully knocked down. Flow cytometry showed that knockdown of Herp arrested granulosa cells at the S phase of the cell cycle. More importantly, ELISA analysis revealed that Herp knockdown significantly upregulated the concentration of estradiol (E2) in the culture supernatants. RT-qPCR was performed to determine the regulatory mechanism of Herp knockdown in the cell cycle, and in steroid synthesis, RT-qPCR analysis revealed that Herp knockdown upregulated the mRNA expression of steroidogenic enzymes (Cyp19a1) and downregulated metabolic enzymes (Cyp1b1) and cell cycle factors (cyclin A1, cyclin B1 and cyclin D2). These results suggest that Herp may regulate the cell cycle and hormone secretions in mouse granulosa cells. The present study helps to elucidate the physiological functions of Herp as they relate to reproduction.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"331 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129459059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2 鸡干细胞因子与成纤维细胞生长因子2协同促进原始生殖细胞增殖

The Journal of Reproduction and Development Pub Date : 2015-12-28 DOI: 10.1262/jrd.2015-128

Daichi Miyahara, Isao Oishi, Ryuichi Makino, Nozomi Kurumisawa, Ryuma Nakaya, T. Ono, H. Kagami, T. Tagami

{"title":"Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2","authors":"Daichi Miyahara, Isao Oishi, Ryuichi Makino, Nozomi Kurumisawa, Ryuma Nakaya, T. Ono, H. Kagami, T. Tagami","doi":"10.1262/jrd.2015-128","DOIUrl":"https://doi.org/10.1262/jrd.2015-128","url":null,"abstract":"An in vitro culture system of chicken primordial germ cells (PGCs) has been recently developed, but the growth factor involved in the proliferation of PGCs is largely unknown. In the present study, we investigated the growth effects of chicken stem cell factor (chSCF) on the in vitro proliferation of chicken PGCs. We established two feeder cell lines (buffalo rat liver cells; BRL cells) that stably express the putative secreted form of chSCF (chSCF1-BRL) and membrane bound form of chSCF (chSCF2-BRL). Cultured PGC lines were incubated on chSCF1 or chSCF2-BRL feeder cells with fibroblast growth factor 2 (FGF2), and growth effects of each chSCF isoform were investigated. The in vitro proliferation rate of the PGCs cultured on chSCF2-BRL at 20 days of culture was more than threefold higher than those cultured on chSCF1-BRL cells and more than fivefold higher than those cultured on normal BRL cells. Thus, use of chSCF2-BRL feeder layer was effective for in vitro proliferation of chicken PGCs. However, the acceleration of PGC proliferation on chSCF2-BRL was not observed without FGF2, suggesting that chSCF2 would act as a proliferation co-factor of FGF2. We transferred the PGCs cultured on chSCF2-BRL cells to recipient embryos, generated germline chimeric chickens and assessed the germline competency of cultured PGCs by progeny test. Donor-derived progenies were obtained, and the frequency of germline transmission was 3.39%. The results of this study demonstrate that chSCF2 induces hyperproliferation of chicken PGCs retaining germline competency in vitro in cooperation with FGF2.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133311612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Developmental competence of oocytes grown in vitro: Has it peaked already? 体外培养卵母细胞的发育能力:已经达到顶峰了吗?

The Journal of Reproduction and Development Pub Date : 2015-12-19 DOI: 10.1262/jrd.2015-148

Kanako Morohaku, Y. Hirao, Y. Obata

引用次数: 17

Challenges to increasing targeting efficiency in genome engineering 提高基因组工程靶向效率的挑战

The Journal of Reproduction and Development Pub Date : 2015-12-18 DOI: 10.1262/jrd.2015-151

T. Horii, I. Hatada

引用次数: 16

MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells MAPK3/1有利于促黄体生成素介导的牛颗粒细胞c型利钠肽的减少

The Journal of Reproduction and Development Pub Date : 2015-12-10 DOI: 10.1262/jrd.2015-135

Lei Yang, Qiang Wei, Junbang Ge, Xiaoe Zhao, B. Ma

引用次数: 8