The Journal of Reproduction and Development最新文献_第9页

Influence of hepatic load from far-off dry period to early postpartum period on the first postpartum ovulation and accompanying subsequent fertility in dairy cows 远干期至产后早期肝负荷对奶牛产后首次排卵及随后的生育能力的影响

The Journal of Reproduction and Development Pub Date : 2016-03-03 DOI: 10.1262/jrd.2015-141

C. Kawashima, Nozomi Ito, S. Nagashima, M. Matsui, K. Sawada, F. Schweigert, A. Miyamoto, K. Kida

{"title":"Influence of hepatic load from far-off dry period to early postpartum period on the first postpartum ovulation and accompanying subsequent fertility in dairy cows","authors":"C. Kawashima, Nozomi Ito, S. Nagashima, M. Matsui, K. Sawada, F. Schweigert, A. Miyamoto, K. Kida","doi":"10.1262/jrd.2015-141","DOIUrl":"https://doi.org/10.1262/jrd.2015-141","url":null,"abstract":"The aim of the present study was to investigate nutritional and metabolic parameters during the dry and early postpartum periods of ovulatory and anovulatory cows, as well as their postpartum reproductive performance. Blood samples from 20 multiparous Holstein cows were collected once a week from the far-off dry period to 3 weeks postpartum. Early postpartum (0–3 weeks) ovulation was confirmed using plasma progesterone concentration profiles, and cows were considered ovulatory if they had resumed luteal activity by this point (n = 9), whereas cows that had not were considered anovulatory (n = 11). Data from the ovulatory and anovulatory cows were analyzed separately for the far-off dry period (7–4 weeks prepartum), the close-up dry period (3–1 weeks prepartum), and the early postpartum period (0–3 weeks). Serum gamma-glutamyl transpeptidase activity (far-off, P = 0.065; close-up, P = 0.051; and early postpartum, P = 0.030) and aspartate aminotransferase (close-up, P = 0.050 and early postpartum, P = 0.087) activities were higher in anovulatory than in ovulatory cows. The days open period was longer (P = 0.019) in anovulatory than in ovulatory cows, and the number of artificial inseminations per conception (P = 0.025) was greater. In conclusion, we found that continuously high gamma-glutamyl transpeptidase activities in serum, which may be induced by liver disorders, prevent subsequent ovulation and affect subsequent fertility, even if cows obtain sufficient ovulation-related energy and β-carotene.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129090448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Application of hollow fiber vitrification for cryopreservation of bovine early cleavage stage embryos and porcine morula-blastomeres 中空纤维玻璃化法在牛卵裂早期胚胎和猪桑葚胚-卵裂球冷冻保存中的应用

The Journal of Reproduction and Development Pub Date : 2016-02-13 DOI: 10.1262/jrd.2015-162

A. Uchikura, H. Matsunari, K. Nakano, Shota Hatae, H. Nagashima

引用次数: 7

N, N-Dimethylglycine decreases oxidative stress and improves in vitro development of bovine embryos N, N-二甲基甘氨酸降低氧化应激，促进牛胚胎体外发育

The Journal of Reproduction and Development Pub Date : 2016-02-11 DOI: 10.1262/jrd.2015-149

Toshikiyo Takahashi, K. Sasaki, T. Somfai, T. Nagai, N. Manabe, K. Edashige

引用次数: 21

Ganglioside GD1a promotes oocyte maturation, furthers preimplantation development, and increases blastocyst quality in pigs 神经节苷脂GD1a促进猪卵母细胞成熟，进一步促进着床前发育，提高囊胚质量

The Journal of Reproduction and Development Pub Date : 2016-02-09 DOI: 10.1262/jrd.2015-083

Jin-Woo Kim, Hyo-Jin Park, Sung-Kyu Chae, Jae-Hyun Ahn, Geon-Yeop Do, Y. Choo, Joung Jun Park, Baedong Jung, Sun-Uk Kim, K. Chang, D. Koo

{"title":"Ganglioside GD1a promotes oocyte maturation, furthers preimplantation development, and increases blastocyst quality in pigs","authors":"Jin-Woo Kim, Hyo-Jin Park, Sung-Kyu Chae, Jae-Hyun Ahn, Geon-Yeop Do, Y. Choo, Joung Jun Park, Baedong Jung, Sun-Uk Kim, K. Chang, D. Koo","doi":"10.1262/jrd.2015-083","DOIUrl":"https://doi.org/10.1262/jrd.2015-083","url":null,"abstract":"Gangliosides are key lipid molecules required for the regulation of cellular processes such as proliferation, differentiation, and cell signaling, including signaling of epidermal growth factor receptor (EGFR). Epidermal growth factor (EGF) has long been considered a potential regulator of meiotic and cytoplasmic maturation in mammalian oocytes. However, there is no report on the direct effect of ganglioside GD1a in porcine oocyte maturation. In this study, we first investigated a functional link between GD1a and meiotic maturation during in vitro maturation (IVM) of porcine embryos. Moreover, we confirmed the effect of exogenous GD1a treatment on blastocyst development, quality, and fertilization rate in early embryonic development. First, we observed that the protein level of ST3GAL2, a GD1a synthesizing enzyme, significantly increased (P < 0.01) in cumulus-oocyte-complexes (COCs) during IVM progress. The proportion of arrested germinal vesicles (GV) increased in oocytes treated with EGF+GD1a (41.6 ± 1.5%) at the IVM I stage. Upon completion of meiotic maturation, the proportion of metaphase II (M II) was significantly higher (P < 0.05) in the EGF+GD1a (89.9 ± 3.6%) treated group. After IVF, the percentage of penetrated oocytes was significantly higher (P < 0.05) in the EGF+GD1a (89.1 ± 2.3%) treated group than in the control group. Furthermore, exogenous GD1a treatment improved the developmental competence and quality of blastocysts during preimplantation embryo development stage. These results suggest that ganglioside GD1a may play an important role in IVM mechanisms of porcine maturation capacity. Furthermore, our findings will be helpful for better promoting the embryo development and blastocyst quality in pigs.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131203724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows 日本黑牛年龄和胎次与血浆巨噬细胞迁移抑制因子浓度呈正相关

The Journal of Reproduction and Development Pub Date : 2016-02-08 DOI: 10.1262/jrd.2015-144

Motoya Koizumi, Asrafun Nahar, R. Yamabe, H. Kadokawa

{"title":"Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows","authors":"Motoya Koizumi, Asrafun Nahar, R. Yamabe, H. Kadokawa","doi":"10.1262/jrd.2015-144","DOIUrl":"https://doi.org/10.1262/jrd.2015-144","url":null,"abstract":"Plasma Macrophage migration inhibitory factor (MIF) concentration correlates positively with age, and negatively with self-rated health in women, and optimal MIF concentration may promote proper reproductive function. This study was conducted to evaluate the hypotheses that plasma MIF concentration changes with parturition or postpartum first ovulation, and that age in months and parity correlate with plasma MIF concentration in Japanese black cows. Western blotting utilizing an anti-MIF mouse monoclonal antibody of various tissues and plasma from females indicated that MIF expression was stronger in the anterior pituitary than in other tissues. We developed a competitive EIA utilizing the same anti-MIF mouse monoclonal antibody with sufficient sensitivity and reliable performance for measuring bovine plasma samples. We then measured MIF concentrations in bovine plasma collected from 4 weeks before parturition to 4 weeks after postpartum first ovulation. There was no significant difference in plasma MIF concentration pre- and post-parturition, or before and after the postpartum first ovulation. Plasma MIF concentrations were positively correlated (P < 0.01) with parity (r = 0.703), age in months on the day of parturition (r = 0.647), and age in months on the day of the postpartum first ovulation (r = 0.553) when we used almost all data, except for that from a third-parity cow with an abnormally high plasma MIF concentration. We therefore concluded that plasma MIF concentrations may increase with age in months and parity, but do not change either before and after parturition or before and after postpartum first ovulation in Japanese black cows.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129678116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An improved method for isolation of epithelial and stromal cells from the human endometrium 一种从人子宫内膜分离上皮细胞和基质细胞的改进方法

The Journal of Reproduction and Development Pub Date : 2016-02-08 DOI: 10.1262/jrd.2015-137

Ayako Masuda, Noriko Katoh, K. Nakabayashi, Kiyoko Kato, K. Sonoda, Mari Kitade, S. Takeda, K. Hata, Junko Tomikawa

{"title":"An improved method for isolation of epithelial and stromal cells from the human endometrium","authors":"Ayako Masuda, Noriko Katoh, K. Nakabayashi, Kiyoko Kato, K. Sonoda, Mari Kitade, S. Takeda, K. Hata, Junko Tomikawa","doi":"10.1262/jrd.2015-137","DOIUrl":"https://doi.org/10.1262/jrd.2015-137","url":null,"abstract":"We aimed to improve the efficiency of isolating endometrial epithelial and stromal cells (EMECs and EMSCs) from the human endometrium. We revealed by immunohistochemical staining that the large tissue fragments remaining after collagenase treatment, which are usually discarded after the first filtration in the conventional protocol, consisted of glandular epithelial and stromal cells. Therefore, we established protease treatment and cell suspension conditions to dissociate single cells from the tissue fragments and isolated epithelial (EPCAM-positive) and stromal (CD13-positive) cells by fluorescence-activated cell sorting. Four independent experiments showed that, on average, 1.2 × 106 of EMECs and 2.8 × 106 EMSCs were isolated from one hysterectomy specimen. We confirmed that the isolated cells presented transcriptomic features highly similar to those of epithelial and stromal cells obtained by the conventional method. Our improved protocol facilitates future studies to better understand the molecular mechanisms underlying the dynamic changes of the endometrium during the menstrual cycle.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131787608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

The efficiency of vaginal temperature measurement for detection of estrus in Japanese Black cows 阴道温度测量法检测日本黑牛发情的有效性

The Journal of Reproduction and Development Pub Date : 2016-02-08 DOI: 10.1262/jrd.2015-095

M. Sakatani, Masashi Takahashi, N. Takenouchi

{"title":"The efficiency of vaginal temperature measurement for detection of estrus in Japanese Black cows","authors":"M. Sakatani, Masashi Takahashi, N. Takenouchi","doi":"10.1262/jrd.2015-095","DOIUrl":"https://doi.org/10.1262/jrd.2015-095","url":null,"abstract":"Recently, weak estrous behavior was assumed to be the cause of a decline in breeding efficiency in cattle. The present study investigated the effect of measuring the vaginal temperature on the detection of estrus in Japanese Black cows. First, the effect of hormone administration to cows with a functional corpus luteum on the vaginal temperature was evaluated by continuous measurement using a temperature data logger. After 24 h of cloprostenol (PG) treatment, the vaginal temperature was significantly lower than on day 7 after estrus, and the low values were maintained until the beginning of estrus (P < 0.05). The cows that received PG and exogenous progesterone (CIDR) did not show a temperature decrease until the CIDR was removed. This finding suggested that the vaginal temperature change reflected the progesterone concentration. The rate of detection of natural estrus was lower for a pedometer than for the vaginal temperature (P < 0.05); synchronization of estrus resulted in a high estrus detection rate regardless of the detection method. In a subsequent experiment, the effect of vaginal temperature measurement and the use of a pedometer on estrus detection was evaluated in the cool and hot seasons. The average activities during non-estrus and the activity increase ratio (estrus/non-estrus) changed according to season (P < 0.01, P < 0.05). However, the average vaginal temperatures during estrus and non-estrus were not affected by season. The estrus detection rate of the pedometer was lower in summer and lower than that obtained using the vaginal temperature. These results indicated that vaginal temperature measurement might be effective for detecting estrus regardless of estrous behavior.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114845710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Selection of accurate reference genes in mouse trophoblast stem cells for reverse transcription-quantitative polymerase chain reaction 小鼠滋养细胞干细胞逆转录-定量聚合酶链反应准确内参基因的选择

The Journal of Reproduction and Development Pub Date : 2016-02-06 DOI: 10.1262/jrd.2015-170

Kaori Motomura, K. Inoue, A. Ogura

{"title":"Selection of accurate reference genes in mouse trophoblast stem cells for reverse transcription-quantitative polymerase chain reaction","authors":"Kaori Motomura, K. Inoue, A. Ogura","doi":"10.1262/jrd.2015-170","DOIUrl":"https://doi.org/10.1262/jrd.2015-170","url":null,"abstract":"Mouse trophoblast stem cells (TSCs) form colonies of different sizes and morphologies, which might reflect their degrees of differentiation. Therefore, each colony type can have a characteristic gene expression profile; however, the expression levels of internal reference genes may also change, causing fluctuations in their estimated gene expression levels. In this study, we validated seven housekeeping genes by using a geometric averaging method and identified Gapdh as the most stable gene across different colony types. Indeed, when Gapdh was used as the reference, expression levels of Elf5, a TSC marker gene, stringently classified TSC colonies into two groups: a high expression groups consisting of type 1 and 2 colonies, and a lower expression group consisting of type 3 and 4 colonies. This clustering was consistent with our putative classification of undifferentiated/differentiated colonies based on their time-dependent colony transitions. By contrast, use of an unstable reference gene (Rn18s) allowed no such clear classification. Cdx2, another TSC marker, did not show any significant colony type-specific expression pattern irrespective of the reference gene. Selection of stable reference genes for quantitative gene expression analysis might be critical, especially when cell lines consisting of heterogeneous cell populations are used.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133140418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

MicroRNA expression and its association with DNA repair in preimplantation embryos 植入前胚胎中MicroRNA的表达及其与DNA修复的关系

The Journal of Reproduction and Development Pub Date : 2016-02-05 DOI: 10.1262/jrd.2015-167

P. Tulay, S. SenGupta

引用次数: 16

Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone β-subunit gene 长链不饱和脂肪酸降低大鼠促卵泡激素β-亚基基因的转录活性

The Journal of Reproduction and Development Pub Date : 2016-02-05 DOI: 10.1262/jrd.2015-138

R. Moriyama, Tsubasa Yamazaki, T. Kato, Y. Kato

{"title":"Long-chain unsaturated fatty acids reduce the transcriptional activity of the rat follicle-stimulating hormone β-subunit gene","authors":"R. Moriyama, Tsubasa Yamazaki, T. Kato, Y. Kato","doi":"10.1262/jrd.2015-138","DOIUrl":"https://doi.org/10.1262/jrd.2015-138","url":null,"abstract":"Here, we assessed the effects of long-chain fatty acids (LCFAs) and the LCFA receptor agonist GW9508 on the transcription of the gonadotropin subunit genes Cga, Lhb and Fshb because LCFA receptor GPR120 was observed in mouse gonadotropes in our recent study. A transcription assay using LβT2 cells demonstrated that LCFAs, oleic acid, α-linolenic acid, docosahexaenoic acid and palmitate, repressed the expression of Cga, Lhb, and Fshb at concentrations between 50 and 100 µM. On the other hand, treatment with 10 µM unsaturated LCFAs, oleic acid, α-linolenic acid and docosahexaenoic acid, repressed only Fshb expression, while the same dose of a saturated LCFA, palmitate, had no effect on the expression of gonadotropin subunit genes. Furthermore, GW9508 did not affect promoter activity. Next, we examined deletion mutants of the upstream region of Fshb and found that the upstream regulatory region (-2824 to -2343 bp) of Fshb was responsible for the notable repression by 10 µM unsaturated LCFAs. Our results suggest that the upstream region of Fshb is susceptible to unsaturated LCFAs. In addition, unsaturated LCFAs play a role in repressing Fshb expression through the distal -2824 to -2343 bp region, which might be independent of the LCFA receptor GPR120 pathway.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"155 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125905760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15