The Journal of Reproduction and Development最新文献_第8页

Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs 在猪卵浆内单精子注射后，缺乏钙振荡导致卵母细胞活化失败

The Journal of Reproduction and Development Pub Date : 2016-09-30 DOI: 10.1262/jrd.2016-113

M. Nakai, J. Ito, Shun'ichi Suzuki, D. Fuchimoto, S. Sembon, Misae Suzuki, J. Noguchi, H. Kaneko, A. Onishi, N. Kashiwazaki, K. Kikuchi

{"title":"Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs","authors":"M. Nakai, J. Ito, Shun'ichi Suzuki, D. Fuchimoto, S. Sembon, Misae Suzuki, J. Noguchi, H. Kaneko, A. Onishi, N. Kashiwazaki, K. Kikuchi","doi":"10.1262/jrd.2016-113","DOIUrl":"https://doi.org/10.1262/jrd.2016-113","url":null,"abstract":"In pigs, the efficiency of embryo production after intracytoplasmic sperm injection (ICSI) is still low because of frequent failure of normal fertilization, which involves formation of two polar bodies and two pronuclei. To clarify the reasons for this, we hypothesized that ICSI does not properly trigger sperm-induced fertilization events, especially intracellular Ca2+ signaling, also known as Ca2+ oscillation. We also suspected that the use of in vitro-matured oocytes might negatively affect fertilization events and embryonic development of sperm-injected oocytes. Therefore, we compared the patterns of Ca2+ oscillation, the efficiency of oocyte activation and normal fertilization, and embryo development to the blastocyst stage among in vivo- or in vitro-matured oocytes after ICSI or in vitro fertilization (IVF). Unexpectedly, we found that the pattern of Ca2+ oscillation, such as the frequency and amplitude of Ca2+ rises, in oocytes after ICSI was similar to that in oocytes after IVF, irrespective of the oocyte source. However, half of the oocytes failed to become activated after ICSI and showed no Ca2+ oscillation. Moreover, the embryonic development of normal fertilized oocytes was reduced when in vitro-matured oocytes were used, irrespective of the fertilization method employed. These findings suggest that low embryo production efficiency after ICSI is attributable mainly to poor developmental ability of in vitro-matured oocytes and a lack of Ca2+ oscillation, rather than the pattern of oscillation.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124895946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes 小鼠精母细胞突触复合体中减数分裂内聚蛋白亚基RAD21L和REC8分别位于外侧元件和横向细丝之间的不同区域

The Journal of Reproduction and Development Pub Date : 2016-09-26 DOI: 10.1262/jrd.2016-127

M. Rong, A. Matsuda, Y. Hiraoka, Jibak Lee

{"title":"Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes","authors":"M. Rong, A. Matsuda, Y. Hiraoka, Jibak Lee","doi":"10.1262/jrd.2016-127","DOIUrl":"https://doi.org/10.1262/jrd.2016-127","url":null,"abstract":"Cohesins containing a meiosis-specific α-kleisin subunit, RAD21L or REC8, play roles in diverse aspects of meiotic chromosome dynamics including formation of axial elements (AEs), assembly of the synaptonemal complex (SC), recombination of homologous chromosomes (homologs), and cohesion of sister chromatids. However, the exact functions of individual α-kleisins remain to be elucidated. Here, we examined the localization of RAD21L and REC8 within the SC by super-resolution microscopy, 3D-SIM. We found that both RAD21L and REC8 were localized at the connection sites between lateral elements (LEs) and transverse filaments (TFs) of pachynema with RAD21L locating interior to REC8 sites. RAD21L and REC8 were not symmetrical in terms of synaptic homologs, suggesting that the arrangement of different cohesins is not strictly fixed along all chromosome axes. Intriguingly, some RAD21L signals, but not REC8 signals, were observed between unsynapsed regions of AEs of zygonema as if they formed a bridge between homologs. Furthermore, the signals of recombination intermediates overlapped with those of RAD21L to a greater degree than with those of REC8. These results highlight the different properties of two meiotic α-kleisins, and strongly support the previous proposition that RAD21L is an atypical cohesin that establishes the association between homologs rather than sister chromatids.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125186461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Adenylation by testis-specific cytoplasmic poly(A) polymerase, PAPOLB/TPAP, is essential for spermatogenesis 睾丸特异性细胞质聚(A)聚合酶(PAPOLB/TPAP)的腺苷化对精子发生至关重要

The Journal of Reproduction and Development Pub Date : 2016-09-18 DOI: 10.1262/jrd.2016-116

S. Kashiwabara, Satsuki Tsuruta, Keitaro Okada, Yutaro Yamaoka, T. Baba

引用次数: 11

Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms 密度梯度离心与游动法相结合，可有效减少形态异常精子

The Journal of Reproduction and Development Pub Date : 2016-09-11 DOI: 10.1262/jrd.2016-112

M. Yamanaka, K. Tomita, S. Hashimoto, Hiroshi Matsumoto, M. Satoh, H. Kato, Y. Hosoi, M. Inoue, Y. Nakaoka, Y. Morimoto

{"title":"Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms","authors":"M. Yamanaka, K. Tomita, S. Hashimoto, Hiroshi Matsumoto, M. Satoh, H. Kato, Y. Hosoi, M. Inoue, Y. Nakaoka, Y. Morimoto","doi":"10.1262/jrd.2016-112","DOIUrl":"https://doi.org/10.1262/jrd.2016-112","url":null,"abstract":"Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fractionated by swim-up following DGC. Ultrastructural abnormalities of sperms obtained from original semen, lower layer of swim-up following DGC, and upper layer of swim-up following DGC were analyzed by transmission electron microscopy. The correlation among ultrastructural head abnormality in sperms from the upper layer of swim-up, fertilization in in vitro fertilization, and pregnancy after embryo transfer was also investigated. Furthermore, sperms with DNA fragmentation in the samples processed via a combination of DGC and swim-up was assessed in a sperm chromatin structure assay. Ultrastructural abnormalities in sperm heads and tails in the upper layer after swim-up following DGC was the lowest among the three groups. Sperms with nuclear vacuoles were the most difficult to eliminate using a combination of DGC and swim-up in all types of head abnormalities. A negative correlation was confirmed between the fertilization rates of intracytoplasmic sperm injection and head abnormality of sperms obtained from the upper layer of the swim-up following DGC. Sperms with DNA fragmentation were effectively decreased using the combination of two techniques. In conclusion, the combination of DGC and swim-up effectively decreased the number of sperms with ultrastructural abnormalities both in the head and in the tail. However, sperms with ultrastructural abnormalities that cannot be completely decreased using a combination of DGC and swim-up may impair fertilization in some cases of intracytoplasmic sperm injection.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132264415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Roles of the reproductive tract in modifications of the sperm membrane surface 生殖道在精子膜表面修饰中的作用

The Journal of Reproduction and Development Pub Date : 2016-03-24 DOI: 10.1262/jrd.2016-028

Yu-Wen Kuo, Sheng-Hsiang Li, K. Maeda, B. Gadella, P. Tsai

{"title":"Roles of the reproductive tract in modifications of the sperm membrane surface","authors":"Yu-Wen Kuo, Sheng-Hsiang Li, K. Maeda, B. Gadella, P. Tsai","doi":"10.1262/jrd.2016-028","DOIUrl":"https://doi.org/10.1262/jrd.2016-028","url":null,"abstract":"Successful fertilization requires viable and functional spermatozoa to recognize and fuse with the oocyte. In most mammalian species, mature spermatozoa are not capable of fertilizing the oocytes immediately after ejaculation. However, unlike somatic cells, spermatozoa, after leaving the testis, are transcriptionally and translationally silent; therefore, upon completion of spermiogenesis, spermatozoa carry only a minimal amount of essential proteins on their membranes as well as within their restricted volume of cytoplasm. To develop into a fully functional and competent sperm that is capable of successful fertilization, modifications of the sperm membrane surface during its transit in the reproductive tracts is critical. These post-spermatogenesis modifications advance the maturation of epididymal spermatozoa. In addition, components secreted into the lumen of the reproductive tracts that are later added onto the sperm membrane surface also regulate (inhibit or activate) the functions of the spermatozoa. This acquisition of additional proteins from the reproductive tracts may compensate for the inactivity of morphologically mature spermatozoa. In this review, we discuss the contributions of the male and female genital tracts to modifications of the sperm membrane surface at different stages of fertilization.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116719158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Functional compensation for the loss of testis-specific poly(A)-binding protein, PABPC2, during mouse spermatogenesis 小鼠精子发生过程中睾丸特异性聚(A)结合蛋白(PABPC2)缺失的功能补偿

The Journal of Reproduction and Development Pub Date : 2016-03-13 DOI: 10.1262/jrd.2016-023

S. Kashiwabara, Satsuki Tsuruta, Keitaro Okada, Ayaka Saegusa, Yu Miyagaki, T. Baba

引用次数: 12

Liver receptor homolog 1 influences blastocyst hatching in pigs 肝受体同源物1影响猪囊胚孵化

The Journal of Reproduction and Development Pub Date : 2016-03-13 DOI: 10.1262/jrd.2015-159

Jing Guo, Ming-hui Zhao, S. Liang, Jeong-woo Choi, Nam-Hyung Kim, X. Cui

{"title":"Liver receptor homolog 1 influences blastocyst hatching in pigs","authors":"Jing Guo, Ming-hui Zhao, S. Liang, Jeong-woo Choi, Nam-Hyung Kim, X. Cui","doi":"10.1262/jrd.2015-159","DOIUrl":"https://doi.org/10.1262/jrd.2015-159","url":null,"abstract":"Liver receptor homolog 1 (Lrh1, also known as Nr5a2) belongs to the orphan nuclear receptor superfamily and has diverse functions in development, metabolism, and cell differentiation and death. Lrh1 regulates the expression of Oct4, which is a key factor of early embryonic differentiation. However, the role of Lrh1 in early development of mammalian embryo is unknown. In the present study, the localization, Lrh1 mRNA expression, and LRH1 protein levels in porcine early parthenotes were examined by immunofluorescence and real-time reverse-transcription polymerase chain reaction. To determine the role of Lrh1 in porcine early embryo development, the parthenotes were treated with the specific LRH1 antagonist 505601. The immunofluorescence signal for LRH1 was only observed in the nucleus of blastocysts. The blastocyst developmental rate in the presence of 50 and 100 μM 505601 was significantly lower than that in the control group. The blastocyst hatching rate was also reduced in the presence of 50 and 100 μM 505601 than that under control conditions. The latter effect was possibly due to the decreased expression of hatching-related genes such as Fn1, Itgα5, and Cox2 upon the inhibition of Lrh1. Incubation with the LRH1 antagonist also increased the number of apoptotic cells among the blastocysts. Moreover, LRH1 inhibition enhanced the expression of the pro-apoptotic genes Bax and Casp3, and reduced the expression of the anti-apoptotic gene Bcl2. Lrh1 inhibition also led to significant decrease in the expression levels of Oct4 mRNA and octamer-binding transcription factor 4 (OCT4) protein in the blastocysts. In conclusion, Lrh1 affects blastocyst formation and hatching in porcine embryonic development through the regulation of OCT4 expression and cell apoptosis.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133651525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro 生长因子在体外诱导大鼠子宫内膜上皮细胞增殖、迁移和管腔形成

The Journal of Reproduction and Development Pub Date : 2016-03-04 DOI: 10.1262/jrd.2015-158

M. Islam, Kazuki Yamagami, Yuka Yoshii, N. Yamauchi

{"title":"Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro","authors":"M. Islam, Kazuki Yamagami, Yuka Yoshii, N. Yamauchi","doi":"10.1262/jrd.2015-158","DOIUrl":"https://doi.org/10.1262/jrd.2015-158","url":null,"abstract":"Endometrial modulation is essential for the preservation of normal uterine physiology, and this modulation is driven by a number of growth factors. The present study investigated the mitogenic, motogenic, and morphogenic effects of epidermal growth factor (EGF) and hepatocyte growth factor (HGF) on rat endometrial epithelial (REE) cells. The REE cells were isolated and cultured and then characterized based on their morphology and their expression of epithelial cell markers. The MTT assay revealed that EGF and HGF induce proliferation of REE cells. Consistent with increased proliferation, we found that the cell cycle regulatory factor Cyclin D1 was also upregulated upon EGF and HGF addition. REE cell migration was prompted by EGF, as observed with the Oris Cell Migration Assay. The morphogenic impact of growth factors on REE cells was studied in a three-dimensional BD Matrigel cell culture system, wherein these growth factors also increased the frequency of lumen formation. In summary, we show that EGF and HGF have a stimulatory effect on REE cells, promoting proliferation, cell migration, and lumen formation. Our findings provide important insights that further the understanding of endometrial regeneration and its regulation.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122990330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Characterization and comparative analyses of transcriptomes for in vivo and in vitro produced peri-implantation conceptuses and endometria from sheep 绵羊着床期胚胎和子宫内膜的体内和体外转录组的特征和比较分析

The Journal of Reproduction and Development Pub Date : 2016-03-04 DOI: 10.1262/jrd.2015-064

Xia Wei, Xiaoling Zhang, M. Kai, Wang Rui, Xu Jing, Guo Min, Zhonghong Wu, Jianhui Tian, Z. Xinyu, An Lei

{"title":"Characterization and comparative analyses of transcriptomes for in vivo and in vitro produced peri-implantation conceptuses and endometria from sheep","authors":"Xia Wei, Xiaoling Zhang, M. Kai, Wang Rui, Xu Jing, Guo Min, Zhonghong Wu, Jianhui Tian, Z. Xinyu, An Lei","doi":"10.1262/jrd.2015-064","DOIUrl":"https://doi.org/10.1262/jrd.2015-064","url":null,"abstract":"An increasing number of reports indicate that in vitro fertilization (IVF) is highly associated with long‑term side effects on embryonic and postnatal development, and can sometimes result in embryonic implant failure. While high‑throughput gene expression analysis has been used to explore the mechanisms underlying IVF-induced side effects on embryonic development, little is known about the effects of IVF on conceptus–endometrial interactions during the peri-implantation period. Using sheep as a model, we performed a comparative transcriptome analysis between in vivo (IVO; in vivo fertilized followed by further development in the uterus) and in vitro produced (IVP; IVF with further culture in the incubator) conceptuses, and the caruncular and intercaruncular areas of the ovine endometrium. We identified several genes that were differentially expressed between the IVO and IVP groups on day 17, when adhesion between the trophoblast and the uterine luminal epithelium begins in sheep. By performing Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, we found that, in the conceptus, differentially expressed genes (DEGs) were associated mainly with functions relating to cell binding and the cell cycle. In the endometrial caruncular area, DEGs were involved in cell adhesion/migration and apoptosis, and in the intercaruncular area, they were significantly enriched in pathways of signal transduction and transport. Thus, these DEGs are potential candidates for further exploring the mechanism underlying IVF/IVP-induced embryonic implant failure that occurs due to a loss of interaction between the conceptus and endometrium during the peri-implantation period.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129076333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Is passive transmission of non-viral vectors through artificial insemination of sperm-DNA mixtures sufficient for chicken transgenesis? 通过人工授精精子- dna混合物的非病毒载体的被动传播是否足以实现鸡的转基因?

The Journal of Reproduction and Development Pub Date : 2016-03-03 DOI: 10.1262/jrd.2015-176

Shahram Chaparian, Ahad Abdulahnejad, Farzad Rashidi, M. Toghyani, A. Gheisari, S. Eghbalsaied

{"title":"Is passive transmission of non-viral vectors through artificial insemination of sperm-DNA mixtures sufficient for chicken transgenesis?","authors":"Shahram Chaparian, Ahad Abdulahnejad, Farzad Rashidi, M. Toghyani, A. Gheisari, S. Eghbalsaied","doi":"10.1262/jrd.2015-176","DOIUrl":"https://doi.org/10.1262/jrd.2015-176","url":null,"abstract":"DNA uptake in the post-acrosomal region of the spermatozoa takes place exclusively in immotile spermatozoa that are naturally unable to fertilize eggs. The present study aimed to assess whether passive transmission of non-viral vectors to the surrounding areas of chicken embryos could be an alternate mechanism in chicken sperm-mediated gene transfer. First, the presence of nucleases in rooster seminal plasma was evaluated. Semen ejaculates from five roosters were centrifuged and the supernatant was incubated with pBL2 for 1 h. A robust nuclease cocktail was detected in the rooster semen. To overcome these nucleases, plasmid-TransIT combinations were incubated with semen for 1 h. Incubation of exogenous DNA in the lipoplex structure could considerably bypass the semen nuclease effect. Then, intravaginal insemination of 1 × 109 sperm mixed with lipoplexes (40 µg pBL2:40 µl TransIT) was carried out in 15 virgin hens. Neither the epithelial tissue from the inseminated female reproductive tracts nor the produced embryos following artificial insemination showed the transgene. To remove any bias in the transgene transmission possibility, the plasmid-TransIT admixture was directly injected in close vicinity of the embryos in newly laid eggs. Nonetheless, none of the produced fetuses or chicks carried the transgene. In conclusion, the results of the present study revealed a nuclease admixture in rooster seminal plasma, and passive/active transmission of the non-viral vector into close vicinity of the chicken embryo was inefficient for producing transgenic chicks.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"208 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134523139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7