The Journal of Reproduction and Development最新文献_第6页

Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase 利用高斯荧光素酶检测l - β t2细胞激素分泌

The Journal of Reproduction and Development Pub Date : 2017-01-15 DOI: 10.1262/jrd.2016-174

K. Satou, Y. Mochimaru, T. Nakakura, T. Kusada, Jun Negishi, Shiori Musha, N. Yoshimura, Y. Kato, H. Tomura

{"title":"Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase","authors":"K. Satou, Y. Mochimaru, T. Nakakura, T. Kusada, Jun Negishi, Shiori Musha, N. Yoshimura, Y. Kato, H. Tomura","doi":"10.1262/jrd.2016-174","DOIUrl":"https://doi.org/10.1262/jrd.2016-174","url":null,"abstract":"Reproduction is regulated by gonadotropins secreted from gonadotrophs. The production and secretion of gonadotropins are mainly regulated by gonadotropin-releasing hormone (GnRH). Agonists or antagonists that influence GnRH action on gonadotrophs are important to regulate reproduction; however, these factors have not been fully characterized due to the lack of simple and easy-to-use techniques to detect gonadotropin secretion from gonadotropin-producing cells. In the present study, we found that Gaussia luciferase (Gluc), which was expressed in LβT2 cells, can be secreted like a luteinizing-hormone (LH) upon stimulation with GnRH. The Gluc secreted into the medium was easily monitored as luminescence signals. The detection range of the GnRH-induced Gluc activity was comparable to that of the enzyme-linked immunosorbent assay for LH. In addition, when the Gluc was expressed in AtT20 cells, which produce adrenocorticotropic hormone (ACTH), the Gluc activity in the medium increased in parallel with the ACTH secretion upon stimulation with corticotropin-releasing hormone. Thus, the Gluc assay in the present study can be easily used for high-throughput screening of factors that influence LH or ACTH secretion from LβT2 or AtT20 cells, respectively.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128524589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Superovulatory responses in cynomolgus monkeys (Macaca fascicularis) depend on the interaction between donor status and superovulation method used 食蟹猴(Macaca fascularis)的超排卵反应取决于供体状态和使用的超排卵方法之间的相互作用

The Journal of Reproduction and Development Pub Date : 2017-01-09 DOI: 10.1262/jrd.2016-074

Ji-Su Kim, Seung-Bin Yoon, K. Jeong, Bo-Woong Sim, Seon-A Choi, Sangil Lee, Y. Jin, B. Song, Sang-Rae Lee, Sun-Uk Kim, K. Chang

{"title":"Superovulatory responses in cynomolgus monkeys (Macaca fascicularis) depend on the interaction between donor status and superovulation method used","authors":"Ji-Su Kim, Seung-Bin Yoon, K. Jeong, Bo-Woong Sim, Seon-A Choi, Sangil Lee, Y. Jin, B. Song, Sang-Rae Lee, Sun-Uk Kim, K. Chang","doi":"10.1262/jrd.2016-074","DOIUrl":"https://doi.org/10.1262/jrd.2016-074","url":null,"abstract":"The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3 ×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases in ovary size and the mean number of MII oocytes retrieved in age- and body-weight-dependent manner; in contrast, both the parameters were similar in Method B [recombinant gonadotrophin (60 IU, twice daily, 6 days), recombinant gonadotropin and recombinant human luteinizing hormone (rhLH) (60 IU, twice daily, 3 days), and hCG]. Importantly, Method A showed maximal MII oocyte recovery rate in > 60-month-old or 4.5–5.0-kg female monkeys, whereas Method B was equally effective regardless of the donor age and body weight. These results indicate that superovulatory responses depend on the interaction between oocyte donor status and the superovulation method used in cynomolgus monkeys.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117186029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Involvement of calcium channels and intracellular calcium in bull sperm thermotaxis 牛精子热致性中钙通道和细胞内钙的参与

The Journal of Reproduction and Development Pub Date : 2016-12-31 DOI: 10.1262/jrd.2016-107

Md Anisuzzaman Mondal, Y. Takagi, S. Baba, K. Hamano

{"title":"Involvement of calcium channels and intracellular calcium in bull sperm thermotaxis","authors":"Md Anisuzzaman Mondal, Y. Takagi, S. Baba, K. Hamano","doi":"10.1262/jrd.2016-107","DOIUrl":"https://doi.org/10.1262/jrd.2016-107","url":null,"abstract":"Thermotaxis that sperm migrate to higher temperature area has been confirmed in rabbit and human. In this study, we examined the migration ability of bull sperm in a temperature gradient to confirm thermotaxis and elucidate the involvement of calcium in such thermotaxis, as well as the relation between sperm capacitation and bull fertility. Thermotaxis was evaluated in a temperature gradient of 34–42ºC using a cross-type column 22-mm long, 40-mm wide, and 100-μm deep. Significantly more sperm migrated to the high-temperature area of 39ºC in a 2ºC temperature gradient, and to 40ºC in a 1ºC temperature gradient. In calcium-free, BAPTA containing medium, and EGTA containing medium, the migrated sperm ratio in the two temperature areas was almost the same. In media containing lanthanum, ruthenium red, and 2APB, we could not confirm thermotaxis. Pre- and post-capacitated sperm migrated to the high-temperature area, expressing thermotaxis. The sperm from high-fertility bulls showed clear thermotaxis. Based on these results, thermotaxis of bull sperm was confirmed and the involvement of both calcium channels and intracellular stored calcium in thermotaxis was suggested. Although the sample size of bulls was quite small, the difference in thermotaxis may have been associated with bull fertility. Sperm thermotaxis evaluation has potential as a predictor of bull fertility.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115788531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares 母马妊娠早中期子宫脂载素2及其受体的表达

The Journal of Reproduction and Development Pub Date : 2016-12-16 DOI: 10.1262/jrd.2016-096

S. Haneda, K. Nagaoka, Y. Nambo, Masato Kikuchi, Y. Nakano, Junyou Li, M. Matsui, Y. Miyake, K. Imakawa

{"title":"Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares","authors":"S. Haneda, K. Nagaoka, Y. Nambo, Masato Kikuchi, Y. Nakano, Junyou Li, M. Matsui, Y. Miyake, K. Imakawa","doi":"10.1262/jrd.2016-096","DOIUrl":"https://doi.org/10.1262/jrd.2016-096","url":null,"abstract":"From previous cDNA subtraction studies analyzing gene expression in equine endometrium, high lipocalin 2 (LCN2) mRNA expression was found in the gravid endometrium. In the uterus, LCN2 may transport hydrophobic molecules and siderophores with iron, or may form a complex with another protein, however, the expression of uterine LCN2 beyond day 20 of equine pregnancy and its receptor has not been characterized. To study the expression and potential roles of uterine LCN2 from pre-implantation to mid-gestation period, stage-specific endometrial samples were obtained from day 13 (day 0 = ovulation) cyclic and days 13, 19, 25, and 60 to 131 pregnant mares. Expression of LCN2 mRNA increased in day 19 gravid endometrium and was abundant from day 60 onward. The expression of LCN2 mRNA was localized to the glandular epithelium. LCN2 protein was detected in day 25 gravid endometrium and luminal fluid, and the protein was localized to the glandular epithelium and luminal cavity, whereas LCN2 receptor expression was found in luminal and glandular epithelium and trophectoderm throughout the experimental period. The presence of matrix metalloproteinase-9 (MMP9) was also examined because MMP9 is known to form a complex with LCN2. Although MMP9 and LCN2 were both found in luminal fluid from day 25 pregnant uterus, the complex of these proteins was not detected. Localization of the receptor in the trophectoderm suggests that endometrial LCN2 could play a role in carrying small substances from the mother to fetus in the equine species.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132519386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Prediction of blastocyst development and implantation potential in utero based on the third cleavage and compaction times in mouse pre-implantation embryos 基于小鼠着床前胚胎第三次卵裂和压实次数的子宫内囊胚发育和着床潜力预测

The Journal of Reproduction and Development Pub Date : 2016-12-16 DOI: 10.1262/jrd.2016-129

Jihyun Kim, S. Kim, J. Jun

{"title":"Prediction of blastocyst development and implantation potential in utero based on the third cleavage and compaction times in mouse pre-implantation embryos","authors":"Jihyun Kim, S. Kim, J. Jun","doi":"10.1262/jrd.2016-129","DOIUrl":"https://doi.org/10.1262/jrd.2016-129","url":null,"abstract":"Cytokinesis and cell division during pre-implantation embryonic development occur as an orchestrated spatiotemporal program. Cleavage, compaction, and blastulation in pre-implantation embryos are essential for successful implantation and pregnancy. Their alteration is associated with chromosomal imbalance and loss of developmental competence. In this study, we evaluated the time of cleavage and compaction as predictors for in vitro pre- and peri-implantation development and in utero implantation potential by time-lapse monitoring. Mouse 2-cell embryos were collected on 1.5 days post coitum (dpc) and were individually cultured to the outgrowth (OG) stage (7.5 dpc). Developmental stages were classified as 3-cell, 4-cell, 8-cell, morula, blastocyst, and OG. Cut-off times for successful blastocyst development were determined by receiver operating characteristic curve analysis. When cut-off times were set as 9 h for the third cleavage from the 2- to 4-cell stage, and 40 h for compaction from the 2-cell to morula stage, blastocyst and OG development rates, respectively, were significantly higher (P < 0.0001). Embryos were grouped according to the above cut-off time and transferred to the contralateral uterine horn on 3.5 dpc. Implantation rates in utero on 5.5 dpc were significantly higher in early third cleaved (≤ 9 h from 2- to 4-cell) and early compacted embryos (≤ 40 h from 2-cell to morula) than those in delayed embryos (P < 0.05). Therefore, the time of the third cleavage from 2- to the 4-cell stage and compaction from 2-cell to morula stage may be a useful morphokinetic parameter for predicting developmental potential, including successful implantation and pregnancy in human in vitro fertilization-embryo transfer programs.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134376060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice IG-DMR中Dlk1-Gtl2位点的保守序列缺失提示它们参与了小鼠父系表达基因的表达

The Journal of Reproduction and Development Pub Date : 2016-12-01 DOI: 10.1262/jrd.2016-135

Takeshi Saito, Satoshi Hara, Moe Tamano, H. Asahara, S. Takada

引用次数: 4

Male infertility-related molecules involved in sperm-oocyte fusion 参与精子-卵细胞融合的男性不育相关分子

The Journal of Reproduction and Development Pub Date : 2016-12-01 DOI: 10.1262/jrd.2016-108

Lisha Mou, Ni Xie

{"title":"Male infertility-related molecules involved in sperm-oocyte fusion","authors":"Lisha Mou, Ni Xie","doi":"10.1262/jrd.2016-108","DOIUrl":"https://doi.org/10.1262/jrd.2016-108","url":null,"abstract":"Male infertility has become a very serious problem in the human reproduction system, but the molecular mechanism of infertility remains largely unknown. Fertilization is the phenomenon in which a sperm and oocyte find each other, interact, and fuse. Sperm-oocyte fusion-related factors on the sperm side play crucial roles in male infertility. For example, IZUMO1 is well-known as a sperm protein essential for fusion of a sperm and oocyte, but its dysfunction or mutation can result in male infertility. Recent studies showed a novel sperm protein named Bactericidal/permeability-increasing protein (BPI), which takes part in the sperm-oocyte fusion process. The complexity and expected redundancy of the factors involved makes the process intricate, with a still poorly understood mechanism, which is difficult to comprehend in full detail. This review summarizes the known molecules involved in the process of sperm-oocyte fusion, mainly focusing on the relevant factors on the sperm side, whose dysregulation may potentially be associated with male infertility. New insights may come from these molecules in this review, can facilitate the development of new treatments of male infertility, and may have a diagnostic value in infertility.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123719146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Overabundance of sika deer and immunocontraception 梅花鹿过多与免疫避孕

The Journal of Reproduction and Development Pub Date : 2016-11-28 DOI: 10.1262/jrd.2016-132

J. Noguchi

引用次数: 4

Development of a lipopolysaccharide (LPS)-supplemented adjuvant and its effects on cell-mediated and humoral immune responses in male rats immunized against sperm 脂多糖(LPS)补充佐剂的研制及其对精子免疫雄性大鼠细胞介导和体液免疫反应的影响

The Journal of Reproduction and Development Pub Date : 2016-11-28 DOI: 10.1262/jrd.2016-144

J. Noguchi, S. Watanabe, T. Q. D. Nguyen, K. Kikuchi, H. Kaneko

{"title":"Development of a lipopolysaccharide (LPS)-supplemented adjuvant and its effects on cell-mediated and humoral immune responses in male rats immunized against sperm","authors":"J. Noguchi, S. Watanabe, T. Q. D. Nguyen, K. Kikuchi, H. Kaneko","doi":"10.1262/jrd.2016-144","DOIUrl":"https://doi.org/10.1262/jrd.2016-144","url":null,"abstract":"Supplementation with lipopolysaccharide (LPS) from non-pathogenic Escherichia coli was found to enhance the adjuvant effects of a veterinary vaccine adjuvant (ISA 71VG®). Sperm immunization using 71VG as an adjuvant in the immature period induced infertility in 25% of male rats, whereas this increased to 62.5% after immunization with 71VG + LPS or Freund′s complete adjuvant (FCA). Mean testicular weight of non-sterile males in the 71VG + LPS group was significantly lower than that in the 71VG or FCA group. Histological examination of testicular tissue from sterile males demonstrated severe impairment of spermatogenesis due to experimental autoimmune orchitis, a cell-mediated autoimmune condition. The serum anti-sperm titer was elevated in the three sperm-immunized groups relative to male rats treated with adjuvant alone, but the titer was higher in the 71VG + LPS and FCA groups than in the 71VG group. We consider that this LPS-supplemented adjuvant stimulates both humoral and cell-mediated immune responses to an extent comparable to FCA.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"353 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125638424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Departure from optimal O2 level for mouse trophoblast stem cell proliferation and potency leads to most rapid AMPK activation 偏离小鼠滋养细胞干细胞增殖和效力的最佳氧水平导致最快速的AMPK激活

The Journal of Reproduction and Development Pub Date : 2016-11-18 DOI: 10.1262/jrd.2016-110

Yu Yang, Zhongliang Jiang, A. Bolnick, Jing Dai, E. Puscheck, D. Rappolee

{"title":"Departure from optimal O2 level for mouse trophoblast stem cell proliferation and potency leads to most rapid AMPK activation","authors":"Yu Yang, Zhongliang Jiang, A. Bolnick, Jing Dai, E. Puscheck, D. Rappolee","doi":"10.1262/jrd.2016-110","DOIUrl":"https://doi.org/10.1262/jrd.2016-110","url":null,"abstract":"Previous studies showed that cultured mouse trophoblast stem cells (mTSCs) have the most rapid proliferation, normal maintenance of stemness/potency, the least spontaneous differentiation, and the lowest level of stress-activated protein kinase (SAPK) when incubated at 2% O2 rather than at the traditional 20% O2 or hypoxic (0.5% and 0% O2) conditions. Switching from 2% O2 induced fast SAPK responses. Here we tested the dose response of AMP-activated protein kinase (AMPK) in its active form (pAMPK Thr172P) at O2 levels from 20–0%, and also tested whether pAMPK levels show similar rapid changes when mTSC cultures were switched from the optimal 2% O2 to other O2 conditions. There was a delayed increase in pAMPK levels ~6–8 h after switching conditions from 20% to 2%, 0.5%, or 0% O2. Altering O2 conditions from 2% to either 20%, 0.5%, or 0% led to rapid increase in pAMPK levels within 1 h, similar to the previously reported SAPK response in mTSC cells removed from 2% O2. Twelve hours of 0.5% O2 exposure led to cell program changes in terms of potency loss and suppressed biosynthesis, as indicated by levels of phosphorylated inactive acetyl CoA carboxylase (pACC). Phosphorylation of ACC was inhibited by the AMPK inhibitor Compound C. However, unlike other stressors, AMPK does not mediate hypoxia-induced potency loss in mTSCs. These results suggest an important aspect of stem cell biology, which demands rapid stress enzyme activation to cope with sudden changes in external environment, e.g., from least stressful (2% O2) to more stressful conditions.","PeriodicalId":416064,"journal":{"name":"The Journal of Reproduction and Development","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129309254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12